Green mode synthesis of silver nanoparticles using Vitis vinifera’s tannin and screening its antimicrobial activity / apoptotic potential versus cancer cells

Silver nanoparticles (SNPs) exhibit tremendous applications in medicine as antimicrobial agent. The use of different parts of plants for the synthesis of nanoparticles is considered as a green technology as it does not involve any harmful chemicals. In the present study, we report a rapid biosynthesis of silver nanoparticles from aqueous leaf extract of medicinal plant Allamanda cathartica. The active phytochemicals present in the plant were responsible for the quick reduction of silver ion to metallic silver nanoparticles. The reduced silver nanoparticles were characterized by using UV-Vis spectrophotometry, Scanning Electron Microscope (SEM), Energy Dispersive Analysis of X-ray (EDAX) and Atomic Force Microscopy (AFM). The spherical shaped silver nanoparticles were observed and it was found to 19-40 nm range of size. These phytosynthesized SNPs were tested for their antimicrobial activity and it analyzed by measuring the inhibitory zone. A. cathartica aqueous leaf extract of SNPs showed highest toxicity to Pseudomonas followed by Klebsiella, Bacillus and E. coli and lowest toxicity towards Proteus. In fungal species, highest inhibition zone was noted against Rhizopus followed by Curvularia, Aspergillus flavus and Aspergillus niger and minimum inhibition zone was observed against Fusarium species. These results suggest a promising potential of Indian plant-based green chemistry for production of SNPs for biomedical and nanotechnology applications.

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Antimicrobial Activity of Silver Nanoparticles Prepared Under an Ultrasonic Field

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2011.4.3.8 ◽

2011 ◽

Vol 4 (3) ◽

pp. 1491-1496

Author(s):

Umadevi M ◽

Rani T ◽

Balakrishnan T ◽

Ramanibai R

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Reduction Method ◽

Therapeutic Potential ◽

Chemical Reduction ◽

Ultrasonic Field ◽

Great Promise ◽

Chemical Reduction Method ◽

E Coli

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms Staphylococcus aureus (MTCC No. 96), Bacillus subtilis (MTCC No. 441), Streptococcus mutans (MTCC No. 497), Escherichia coli (MTCC No. 739) and Pseudomonas aeruginosa (MTCC No. 1934). The results shows that B. subtilis, and E. coli were more sensitive to silver nanoparticles and its size, indicating the superior antimicrobial efficacy of silver nanoparticles.

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Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2012.5.1.7 ◽

2012 ◽

Vol 5 (1) ◽

pp. 1638-1642 ◽

Cited By ~ 2

Author(s):

Vidyasagar G M ◽

Shankaravva B ◽

R Begum ◽

Imrose ◽

Sagar R ◽

...

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Silver Ions ◽

Human Beings ◽

Streptomyces Species ◽

Force Microscopy ◽

E Coli ◽

Extracellular Synthesis ◽

Atomic Force ◽

Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

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Green and Simple Synthesis of Silver Nanoparticles by Aqueous Extract of Perovskia abrotanoides: Characterization, Optimization and Antimicrobial Activity

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190618121218 ◽

2020 ◽

Vol 21 (11) ◽

pp. 1129-1137 ◽

Cited By ~ 1

Author(s):

Somayeh Mirsadeghi ◽

Masoumeh F. Koudehi ◽

Hamid R. Rajabi ◽

Seied M. Pourmortazavi

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Minimum Inhibitory Concentration ◽

Plant Extract ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Extraction Temperature ◽

Silver Particles ◽

Silver Nps ◽

Perovskia Abrotanoides

Background: Herein, we report the biosynthesis procedure to prepare silver nanoparticles as reduction and capping agents with the aqueous plant extract of Perovskia abrotanoides. Methods: The therapeutic application of silver nanoparticles entirely depends on the size and shape of the nanoparticles therefore, their control during the synthesis procedure is so important. The effects of synthesis factors, for example, silver ion concentration, the mass of plant extract, reaction time and extraction temperature, on the size of silver particles were considered and optimized. Several analytical methods were used for the characterization of silver NPs including FT-IR and UV–Vis spectrophotometer, XRD and SEM. Results: The results showed that the mean size of the silver particles was about 51 nm. Moreover, the antibacterial properties of biosynthesized silver NPs were investigated by the minimum inhibitory concentration, minimum bactericidal concentration, and Well-diffusion tests. The minimum inhibitory concentration/ minimum bactericidal concentration values of silver NPs and aqueous plant extract versus Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and Gram-negative bacteria (E. coli) were 3.03/0.00, 1.20/0.01, 3.06/0.00, 0.98/1.04, 1.00/0.05 and 1.30/0.03 (mg/mL), respectively. Conclusion: The antimicrobial activity study displayed that the synthesized silver nanoparticles by plant extract have better antimicrobial properties compared to aqueous plant extract of Perovskia abrotanoides.

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Synthesis of Silver Nanoparticles Using Flaxseed Hydroalcoholic Extract and its Antimicrobial Activity

Current Biotechnology ◽

10.2174/22115501113029990007 ◽

2013 ◽

Vol 2 (2) ◽

pp. 162-166 ◽

Cited By ~ 6

Author(s):

Archana Sharbidre ◽

Deepak Kasote

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Hydroalcoholic Extract

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Bio Mediated Synthesis of Silver Nanoparticles using Moringa Oleifera Bark Extract and its Antimicrobial Activity against Human Pathogens

International Journal for Research in Applied Science and Engineering Technology ◽

10.22214/ijraset.2018.4362 ◽

2018 ◽

Vol 6 (4) ◽

pp. 2108-2112

Author(s):

T. Varthini

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Moringa Oleifera ◽

Bark Extract ◽

Human Pathogens

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Anticancer Potential of Biogenic Silver Nanoparticles: A Mechanistic Study

Pharmaceutics ◽

10.3390/pharmaceutics13050707 ◽

2021 ◽

Vol 13 (5) ◽

pp. 707

Author(s):

Mohd Shahnawaz Khan ◽

Alya Alomari ◽

Shams Tabrez ◽

Iftekhar Hassan ◽

Rizwan Wahab ◽

...

Keyword(s):

Silver Nanoparticles ◽

Cancer Cells ◽

Cell Lines ◽

Human Life ◽

Mechanistic Study ◽

Dependent Manner ◽

Rt Pcr ◽

Cell Viability Assay ◽

Hct 116 ◽

Mcf 7

The continuous loss of human life due to the paucity of effective drugs against different forms of cancer demands a better/noble therapeutic approach. One possible way could be the use of nanostructures-based treatment methods. In the current piece of work, we have synthesized silver nanoparticles (AgNPs) using plant (Heliotropiumbacciferum) extract using AgNO3 as starting materials. The size, shape, and structure of synthesized AgNPs were confirmed by various spectroscopy and microscopic techniques. The average size of biosynthesized AgNPs was found to be in the range of 15 nm. The anticancer potential of these AgNPs was evaluated by a battery of tests such as MTT, scratch, and comet assays in breast (MCF-7) and colorectal (HCT-116) cancer models. The toxicity of AgNPs towards cancer cells was confirmed by the expression pattern of apoptotic (p53, Bax, caspase-3) and antiapoptotic (BCl-2) genes by RT-PCR. The cell viability assay showed an IC50 value of 5.44 and 9.54 µg/mL for AgNPs in MCF-7 and HCT-116 cell lines respectively. We also observed cell migration inhibiting potential of AgNPs in a concentration-dependent manner in MCF-7 cell lines. A tremendous rise (150–250%) in the production of ROS was observed as a result of AgNPs treatment compared with control. Moreover, the RT-PCR results indicated the difference in expression levels of pro/antiapoptotic proteins in both cancer cells. All these results indicate that cell death observed by us is mediated by ROS production, which might have altered the cellular redox status. Collectively, we report the antimetastasis potential of biogenic synthesized AgNPs against breast and colorectal cancers. The biogenic synthesis of AgNPs seems to be a promising anticancer therapy with greater efficacy against the studied cell lines.

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Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities

Journal of Marine Science and Engineering ◽

10.3390/jmse9060678 ◽

2021 ◽

Vol 9 (6) ◽

pp. 678

Author(s):

Kaliyamoorthy Kalidasan ◽

Nabikhan Asmathunisha ◽

Venugopal Gomathi ◽

Laurent Dufossé ◽

Kandasamy Kathiresan

Keyword(s):

Antimicrobial Activity ◽

Gold Nanoparticles ◽

Silver Nanoparticles ◽

Biomass Production ◽

Petroleum Ether Extract ◽

Antimicrobial Activities ◽

Culture Conditions ◽

Gold And Silver Nanoparticles ◽

Optimization Of Culture Conditions ◽

Antioxidant And Antimicrobial Activities

This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity.

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