S142 – Circulating Levels of CD105 and VEGF in Oral Cancer

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P124-P124
Author(s):  
Chih-Yen Chien ◽  
Chao-Cheng Huang
Keyword(s):  
Oral Cancer ◽  
Normal Control ◽  
Tumor Stage ◽  
Normal Control Group ◽  
Nodal Status ◽  
Stage I ◽  
Stage Iii ◽  
Control Group ◽  
The Difference ◽  
Circulating Levels

Objectives To evaluate the circulating levels of CD105 and VEGF in patients with oral cancer and analyze the correlation between these circulating levels and clinicopathological features. Methods Between 2006 January and 2007 December, 28 healthy volunteers and 68 patients with squamous cell carcinoma of oral cavity (OSCC) were enrolled in this study. The serum level of VEGF and plasma level of CD105 were determined by ELISA method, respectively, from the frozen stored blood before treatment. ANOVA analysis was used to evaluate the difference of circulating levels of CD105 and VEGF between normal control group and OSCC group. The difference between nodal status (positive versus negative), tumor stage (T1, T2 versus T3, T4) and TNM stage (stage I, II vs. stage III, IV) were also evaluated. Results The circulating levels of CD105 and VEGF in normal control group were 1.457 ± 0.46 ng/ml and 44.89 ± 20.4 pg/ml, respectively. The circulating levels of CD105 and VEGF in OSCC group were 7.056 ± 3.44 ng/ml and 292.94 ± 117.3 pg/ml, respectively. Both circulating levels of CD105 and VEGF in patients with OSCC were significantly higher than those of normal control group (both p<0.001). In addition, the patients with positive nodal status (p<0.001), advanced tumor stage (T3, T4 versus T1, T2) (p<0.001), and advanced TNM stage (stage III, IV versus stage I, II) (p<0.001) had significantly higher circulating levels of CD105 and VEGF. Conclusions Both circulation levels of CD105 and VEGF are useful markers to evaluate the disease progression of OSCC.

scholarly journals Changes of Peripheral Blood Lymphocyte Subsets and Cytokines in Patients with Multiple Myeloma and Their Clinical Significance

Blood ◽  
2020 ◽  
Vol 136 (Supplement 1) ◽  
pp. 38-38
Author(s):  
Li Fei
Keyword(s):  
T Cells ◽  
Normal Control ◽  
Cd4 T Cells ◽  
Lymphocyte Subsets ◽  
Risk Group ◽  
Normal Control Group ◽  
Control Group ◽  
Newly Diagnosed ◽  
Significant Difference ◽  
The Difference

Objective: Accumulating evidence have indicated that immune response play an essential role in development of multiple myeloma (MM),To evaluate the immune function of patients with MM by detecting the levels of lymphocyte subsets and cytokines, and to analyze the correlation between the immune function and prognosis of patients. Methods: we examined 50 patients with newly diagnosed MM, 20 patients with Relapse / refractory MM and 30 healthy volunteers. the levels of T lymphocyte subsets, activated T cells subtypes, Treg and cytokines in peripheral blood were detected by flow cytometry (FCM).Results: ① compared with healthy control group, CD3 + CD4 + T cells of newly diagnosed MM patients were significantly decreased (P = 0.04), There was no significant difference in CD3+CD8+T and CD4/CD8 of newly diagnosed MM patients with normal controls (P=0.14).The percentage of CD16 + cd56dim NK cells of newly diagnosed MM was significantly lower than that of the normal control group (P = 0.01); There was no significant difference in frequencies of CD3+CD4+T cells, CD4/CD8, CD16+CD56dimNK (P=0.34, P=0.561, P=0.88) between newly treated MM patients and relapsed/refractory MM; HLA-DR-CD8+ activated TS cells of newly diagnosed MM was significantly higher than the normal control group (P = 0.04).② The proportion of CD4 + CD25 + CD127dim T cells(treg) in total CD4 + T cells in of newly diagnosed MM was higher than that in normal control group (P &lt; 0.05). The CD4 + CD25 +CD127dim T cells of MM patients with relapse / refractory MM was higher than that of newly treated patients, but the difference was not statistically significant (P &gt; 0.05). Further analysis showed that CD4 + CD25 + CD127dim T cells in mSMART stratified high-risk group were higher than those in low-risk group (P = 0.03). The number of CD4 + CD25 + CD127dim T cells in patients without PR after 2 courses of PAD was higher than that in patients with≥ PR, but the difference was not statistically significant (P &gt; 0.05). ③ The levels of IL-6, IL-8, TNF - α of newly diagnosed MM were significantly higher than normal control group, and were positively correlated with ISS stage (r=0.61, r=0.67, r=0.59, P均&lt;0.05).④ The levels of IL-6 and IL-8 of newly diagnosed MM with renal injury were significantly higher than those in patients with normal renal function (P &lt; 0.05). Further studies showed that the level of IL-8 was negatively correlated with the proportion of CD16 + CD56dimNK cells (r=-0.65, P&lt;0.05).Conclusion: the abnormal expression of lymphocyte subsets and cytokine levels in MM patients may be related to the tumor load, disease progression and prognosis. Disclosures No relevant conflicts of interest to declare.

scholarly journals Study on potential differentially expressed genes in stroke by bioinformatics analysis

2021 ◽  
Author(s):  
Xitong Yang ◽  
Pengyu Wang ◽  
Shanquan Yan ◽  
Guangming Wang
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Normal Control ◽  
Enrichment Analysis ◽  
Normal Control Group ◽  
Control Group ◽  
Ppi Network ◽  
Hub Genes ◽  
Pathway Enrichment ◽  
Key Genes

AbstractStroke is a sudden cerebrovascular circulatory disorder with high morbidity, disability, mortality, and recurrence rate, but its pathogenesis and key genes are still unclear. In this study, bioinformatics was used to deeply analyze the pathogenesis of stroke and related key genes, so as to study the potential pathogenesis of stroke and provide guidance for clinical treatment. Gene Expression profiles of GSE58294 and GSE16561 were obtained from Gene Expression Omnibus (GEO), the differentially expressed genes (DEGs) were identified between IS and normal control group. The different expression genes (DEGs) between IS and normal control group were screened with the GEO2R online tool. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the DEGs were performed. Using the Database for Annotation, Visualization and Integrated Discovery (DAVID) and gene set enrichment analysis (GSEA), the function and pathway enrichment analysis of DEGS were performed. Then, a protein–protein interaction (PPI) network was constructed via the Search Tool for the Retrieval of Interacting Genes (STRING) database. Cytoscape with CytoHubba were used to identify the hub genes. Finally, NetworkAnalyst was used to construct the targeted microRNAs (miRNAs) of the hub genes. A total of 85 DEGs were screened out in this study, including 65 upward genes and 20 downward genes. In addition, 3 KEGG pathways, cytokine − cytokine receptor interaction, hematopoietic cell lineage, B cell receptor signaling pathway, were significantly enriched using a database for labeling, visualization, and synthetic discovery. In combination with the results of the PPI network and CytoHubba, 10 hub genes including CEACAM8, CD19, MMP9, ARG1, CKAP4, CCR7, MGAM, CD79A, CD79B, and CLEC4D were selected. Combined with DEG-miRNAs visualization, 5 miRNAs, including hsa-mir-146a-5p, hsa-mir-7-5p, hsa-mir-335-5p, and hsa-mir-27a- 3p, were predicted as possibly the key miRNAs. Our findings will contribute to identification of potential biomarkers and novel strategies for the treatment of ischemic stroke, and provide a new strategy for clinical therapy.

Effect of GDM on the Expression of MT1-MMP and u-PA in Glioma Cells

2014 ◽  
Vol 1033-1034 ◽  
pp. 220-223
Author(s):  
Xue Mei Han ◽  
Li Bo Wang ◽  
Ni Ni Li ◽  
Song Yan Liu
Keyword(s):  
Normal Control ◽  
Glioma Cell ◽  
Fetal Bovine Serum ◽  
Glioma Cells ◽  
Normal Control Group ◽  
Control Group ◽  
Rt Pcr ◽  
Glioma Cell Lines ◽  
Fetal Bovine ◽  
Identify Gene Expression

To examine the effect of GDM on the expression of MT1-MMP and u-PA genes in glioma cells. Glioma cell lines U251 and U87 were cultured in DMEM medium supplemented with 10% fetal bovine serum. RT-PCR was used to identify gene expression level. The level of u-PA mRNA was up-regulated significantly in the HGF group compared with the normal control group (P<0.05). The expression of MT1-MMP and u-PA was significantly lower in the GDM group than in the normal control and HGF groups (P<0.05). The expression of u-PA in the HGF+GDM group was down-regulated significantly compared with the normal control and HGF groups (P<0.05).GDM can inhibit expression of both MT1-MMP and u-PA in glioma cells.

Identification of Hub Genes Associated with Development of Lung Adenocarcinoma by Integrated Bioinformatics Analysis

2021 ◽  
Author(s):  
Jinglei Li ◽  
Wei Hou
Keyword(s):  
Gene Expression ◽  
Survival Analysis ◽  
Network Analysis ◽  
Lung Adenocarcinoma ◽  
Normal Control ◽  
Gene Expression Analysis ◽  
Normal Control Group ◽  
Control Group ◽  
Differential Gene Expression Analysis ◽  
Differential Gene

Abstract Purpose: Lung adenocarcinoma (LUAD) has high heterogeneity and poor prognosis, posing a major challenge to human health worldwide. Therefore, it is necessary to improve our understanding of the molecular mechanism of LUAD in order to be able to better predict its prognosis and develop new therapeutic strategies for target genes.Methods: The Cancer Genome Atlas and Gene Expression Omnibus, were selected to comprehensively analyze and explore the differences between LUAD tumors and adjacent normal tissues. Critical gene information was obtained through weighted gene co-expression network analysis (WGCNA), differential gene expression analysis, and survival analysis.Results: Using WGCNA and differential gene expression analysis, 29 differentially expressed genes were screened. The functional annotation analysis showed these genes to be mainly concentrated in heart trabecula formation, regulation of inflammatory response, collagen-containing extracellular matrix, and metalloendopeptidase inhibitor activity. Also, in the protein–protein interaction network analysis, 10 central genes were identified using Cytoscape's CytoHubba plug-in. The expression of CDH5, TEK, TIMP3, EDNRB, EPAS1, MYL9, SPARCL1, KLF4, and TGFBR3 in LUAD tissue was found to be lower than that in the normal control group, while the expression of MMP1 in LUAD tissue was higher than that in the normal control group. According to survival analysis, the low expression of MYL9 and SPARCL1 was correlated with poor overall survival in patients with LUAD. Finally, through the verification of the Oncomine database, it was found that the expression levels of MYL9 and SPARCL1 were consistent with the mRNA levels in LUAD samples, and both were downregulated.Conclusion: Two survival-related genes, MYL9 and SPARCL1, were determined to be highly correlated with the development of LUAD. Both may play an essential role in the development LUAD and may be potential biomarkers for its diagnosis and treatment in the future.

scholarly journals Hepatotoxicity and Histological Evaluation of Aqueous and Methanolic Leaf Extracts of Thaumatococcus Daniellii and Alchornea Cordifolia in Wistar Rat Models

2019 ◽  
Vol 1 (2) ◽  
pp. p42
Author(s):  
Service @ Ideasspread.org ◽  
Okafor I. J. ◽  
Nweke E. O. ◽  
Ewa O.
Keyword(s):  
Normal Control ◽  
Wistar Rat ◽  
Normal Control Group ◽  
Histological Evaluation ◽  
Control Group ◽  
Significant Elevation ◽  
Leaf Extracts ◽  
Group Iii ◽  
Methanolic Extracts ◽  
Group I

This study was carried out to ascertain the hepatotoxic potential of T.daniellii (T.d) and A. cordifolia (A.c). Investigations were conducted using standard methods. Oral administration of 200mg/kg aqueous leaf extracts of T.daniellii caused a non-significant increase in the activity of ALT (5.43±0.60IU/L), AST (16.93±0.26 IU/L) and ALP (160.70±1.04 IU/L) compared to the values recorded on the normal control (group I) ALT (3.84±0.16 IU/L), AST (14.19±0.52 IU/L) and ALP (157.26±0.64 IU/L). Group III administered with 200mg/kg methanolic leaf extract of T. daniellii manifested a significant elevation in the activity of ALT (13.15±0.89 IU/L), AST (22.84±0.38 IU/L) and ALP (170.40±0.44 IU/L) compared to the normal control. Similarly, groups IV and V which were orally administered with 200mg/kg aqueous and methanolic leaf extracts of A. cordifolia showed significant increase in the activity of ALT (6.32±0.33U/L), AST (17.70±0.030U/L) and ALP (161.13±0.09U/L) and ALT (7.55±0.59U/L), AST (19.35±0.26U/L) and ALP (165.38±0.35U/L) respectively compared to the values recorded on the control (group I). In conclusion, drug development protocols involving T. daniellii leaf should preferably use water as an ideal solvent. On the other hand, the hepatotocity associated with both aqueous and methanolic extracts of A. cordifolia could imply the presence of hepatotoxins in the leaf of the said plant.

The Mechanism of Proboscis Movement in Arenicola

1954 ◽  
Vol s3-95 (30) ◽  
pp. 251-270
Author(s):  
G. P. WELLS
Keyword(s):  
Body Fluid ◽  
Body Wall ◽  
The Body ◽  
Stage I ◽  
Stage Iii ◽  
Forward Movement ◽  
Arenicola Marina ◽  
Buccal Mass ◽  
The Difference ◽  
Longitudinal Muscles

The mechanism of proboscis movement is analysed in detail in Arenicola marina L. and A. ecaudata Johnston, and discussed in relation to the properties of the hydrostatic skeleton. Proboscis activity is based on the following cycle of movements in both species. Stage I. The circular muscles of the body-wall and buccal mass contract; the head narrows and lengthens. Stage IIa. The circular muscles of the mouth and buccal mass relax; the gular membrane (or ‘first diaphragm’ of previous authors) contracts; the mouth opens and the buccal mass emerges. Stage IIb. The longitudinal muscles of the buccal mass and body-wall contract; the head shortens and widens and the pharynx emerges. Stage III. As Stage I. The two species differ anatomically and in their hydrostatic relationships. In ecaudata, the forward movement of body-fluid which extrudes and distends the proboscis is largely due to the contraction of the gular membrane and septal pouches. In marina, the essential mechanism is the relaxation of the oral region which allows the general coelomic pressure to extrude the proboscis. The gular membrane of marina contracts as that of ecaudata does, but its anatomy is different and it appears to be a degenerating structure as far as proboscis extrusion is concerned. Withdrawal of the proboscis may occur while the head is still shortening and widening in Stage IIb, or while it is lengthening and narrowing in Stage III. The proboscis is used both in feeding and in burrowing; in the latter case nothing enters through the mouth; the difference is largely caused by variation in the timing of withdrawal relative to the 3-stage cycle.

scholarly journals ACUTE TOXICOLOGICAL INVESTIGATION OF POLYETHYLENE GLYCOL DERIVATIZED FOURTH AND FIFTH GENERATION POLY (PROPYLENEIMINE) DENDRIMERS

2019 ◽  
pp. 222-229 ◽  
Author(s):  
NITIN DWIVEDI ◽  
DUSHYANT KUMAR PARMAR ◽  
PRASHANT KESHARWANI ◽  
JIGNA SHAH
Keyword(s):  
Polyethylene Glycol ◽  
Normal Control ◽  
Normal Control Group ◽  
Control Group ◽  
Severe Toxicity ◽  
Hemoglobin Content ◽  
Dose Administration ◽  
Fifth Generation ◽  
Wbc Count ◽  
Rbc Count

Objective: The aim of the present study leads a comparative assessment of the toxicological profile of PEGylated fourth and fifth-generation poly (propylene imine) dendrimers (PPI). Methods: 4.0G and 5.0G generations of PPI dendrimer were synthesized and PEGylated with Mono polyethylene glycol 5000 (MPEG-5000). Each PEGylated 4.0G and 5.0G dendrimeric generation were administered in three different doses: 2.5 mg/kg, 25 mg/kg and 250 mg/kg (i.e., low, intermediate and high dose) to wister rats. After the dose administration, the blood and tissue samples of wister rats were collected after 24 h and 15 d after. All the collected samples were proceeded for hematological, biochemical and histopathological studies. Results: After 24 h of (250 mg/kg) dose administration PEGylated 5.0G PPI dendrimer the RBC count, hemoglobin content and WBC count were found 7.873±0.129 mill/cmm, 13.833±0.491g/dl and 9033.33±2384.906 mill/cmm, while PEGylated 4.0G PPI dendrimer indicated RBC count, hemoglobin content and WBC count 8.733±0.239 mill/cmm, 14.033±0.12 g/dl and 9666.667±2567.316 mill/cmm, in blood samples as compare to RBC count 9.346±0.037 mill/cmm, hemoglobin content 15.35±0.15 g/dl and WBC count 8500±286.675 mill/cmm of the animals of normal control group. Thus there are no remarkable changes (p>0.05) in RBC count, hemoglobin content and other hematological profile after 24 h in comparison of normal control group of animals. Similarily insignificant changes (p>0.05) in serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), lactate dehydrogenase (LDH) and sections of different organs indicate inoffensive nature of both generations of PEGylated 4.0G and 5.0 G PPI dendrimers. Conclusion: It can be concluded that fifth-generation PPI dendrimers are more suitable as compared to fourth generation of PPI dendrimer, while both dendrimers are not generating any severe toxicity.

scholarly journals Elevated levels of proinflammatory and anti-inflammatory cytokines in patients with bladder cancer depending on a tumor stage

2020 ◽  
Vol 93 (1) ◽  
Author(s):  
Viktor Dmytryk ◽  
Tetiana Luhovska ◽  
Pavel Yakovlev ◽  
Olexiy Savchuk ◽  
Ludmila Ostapchenko ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Stage Iv ◽  
Tumor Stage ◽  
Stage I ◽  
Stage Ii ◽  
Stage Iii ◽  
Common Disease ◽  
Tnf Α ◽  
Ifn Γ ◽  
Th 1

Bladder Cancer (BC) is a common disease worldwide. Chronic inflammation is one of the key mechanisms for the development of BC. This study enrolled 40 patients. Preoperative plasma levels of IL-1β, IL-4, IL-6, IL-10, IL-12β, TNF-α and IFN-γ were determined by ELISA. In our study, we observed diverse changes in the levels of cytokines in patients with BC Stage I, II, III and IV. The levels of IL-1β was increased for stage I, stage II, and stage III. The level of TNF-α was increased for stage II, stage III, stage IV. The levels of IL-4, IL-6, IL-10 and IL-12β were increased in patients with stage III and IV only. The levels of IFN- γ declined for stage II, stage III and stage IV with the lowest levels in patients with Stage IV. In our study, we investigated alteration in levels of Th-1 and Th-2-like cytokine profile, but some deficiency in Th1- status discovered in patients with BC.

Quality of Parenting in Alcoholics and Narcotic Addicts

1989 ◽  
Vol 154 (5) ◽  
pp. 677-682 ◽  
Author(s):  
Elsa Bernardi ◽  
Michael Jones ◽  
Chris Tennant
Keyword(s):  
Normal Control ◽  
Parental Bonding ◽  
Normal Control Group ◽  
Control Group ◽  
Heroin Addicts ◽  
Parental Bonding Instrument ◽  
Paternal Parenting ◽  
Quality Of Parenting

Alcoholics and heroin addicts were compared with a normal control group to determine whether there were differences in quality of parenting during childhood, assessed using the Parental Bonding Instrument. Maternal and paternal overprotection were reported more commonly by narcotic addicts. Maternal overprotection alone was implicated in alcoholics. Narcotic addicts seem to have more disturbed parenting than alcoholics, especially paternal parenting.

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