Quorum sensing in the Burkholderia cepacia complex

2004 ◽  
Vol 155 (4) ◽  
pp. 238-244 ◽  
Author(s):  
Vittorio Venturi ◽  
Arianna Friscina ◽  
Iris Bertani ◽  
Giulia Devescovi ◽  
Claudio Aguilar
Keyword(s):  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
Burkholderia Cepacia Complex

scholarly journals Distribution of Quorum-Sensing Genes in the Burkholderia cepacia Complex

2001 ◽  
Vol 69 (7) ◽  
pp. 4661-4666 ◽  
Author(s):  
E. Lutter ◽  
S. Lewenza ◽  
J. J. Dennis ◽  
M. B. Visser ◽  
P. A. Sokol
Keyword(s):  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
Homoserine Lactone ◽  
Burkholderia Cepacia Complex ◽  
Homoserine Lactones

ABSTRACT The distribution of quorum-sensing genes among strains from seven genomovars of the Burkholderia cepaciacomplex was examined by PCR. cepR and cepI were amplified from B. cepacia genomovars I and III, B. stabilis, and B. vietnamiensis. cepR was also amplified from B. multivorans and B. cepacia genomovar VI. bviIR were amplified from B. vietnamiensis. All genomovars producedN-octanoyl-l-homoserine lactone andN-hexanoyl-l-homoserine lactone.B. vietnamiensis and B. cepacia genomovar VII produced additional N-acyl-l-homoserine lactones.

scholarly journals Structural and Functional Characterization of Diffusible Signal Factor Family Quorum-Sensing Signals Produced by Members of the Burkholderia cepacia Complex

2010 ◽  
Vol 76 (14) ◽  
pp. 4675-4683 ◽  
Author(s):  
Yinyue Deng ◽  
Ji'en Wu ◽  
Leo Eberl ◽  
Lian-Hui Zhang
Keyword(s):  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
High Performance ◽  
Xanthomonas Campestris ◽  
Functional Characterization ◽  
Cell Communication ◽  
Burkholderia Cenocepacia ◽  
Burkholderia Cepacia Complex ◽  
Communication Signals ◽  
Diffusible Signal Factor

ABSTRACT Previous work has shown that Burkholderia cenocepacia produces the diffusible signal factor (DSF) family signal cis-2-dodecenoic acid (C12:Δ2, also known as BDSF), which is involved in the regulation of virulence. In this study, we determined whether C12:Δ2 production is conserved in other members of the Burkholderia cepacia complex (Bcc) by using a combination of high-performance liquid chromatography, mass spectrometry, and bioassays. Our results show that five Bcc species are capable of producing C12:Δ2 as a sole DSF family signal, while four species produce not only C12:Δ2 but also a new DSF family signal, which was identified as cis,cis-11-methyldodeca-2,5-dienoic acid (11-Me-C12:Δ2,5). In addition, we demonstrate that the quorum-sensing signal cis-11-methyl-2-dodecenoic acid (11-Me-C12:Δ2), which was originally identified in Xanthomonas campestris supernatants, is produced by Burkholderia multivorans. It is shown that, similar to 11-Me-C12:Δ2 and C12:Δ2, the newly identified molecule 11-Me-C12:Δ2,5 is a potent signal in the regulation of biofilm formation, the production of virulence factors, and the morphological transition of Candida albicans. These data provide evidence that DSF family molecules are highly conserved bacterial cell-cell communication signals that play key roles in the ecology of the organisms that produce them.

scholarly journals Functional Quorum Sensing Systems are Maintained during Chronic Burkholderia cepacia Complex Infections in Patients with Cystic Fibrosis

2011 ◽  
Vol 203 (3) ◽  
pp. 383-392 ◽  
Author(s):  
Suzanne A. McKeon ◽  
David T. Nguyen ◽  
Duber F. Viteri ◽  
James E. A. Zlosnik ◽  
Pamela A. Sokol
Keyword(s):  
Cystic Fibrosis ◽  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
Burkholderia Cepacia Complex ◽  
Sensing Systems

Sulfonamide-based diffusible signal factor analogs interfere with quorum sensing in Stenotrophomonas maltophilia and Burkholderia cepacia

2019 ◽  
Vol 11 (13) ◽  
pp. 1565-1582 ◽  
Author(s):  
Pol Huedo ◽  
Vydyula P Kumar ◽  
Conor Horgan ◽  
Daniel Yero ◽  
Xavier Daura ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
Stenotrophomonas Maltophilia ◽  
Multidrug Resistant ◽  
Burkholderia Cepacia Complex ◽  
Antibiofilm Activity ◽  
Gram Negative ◽  
Alternative Approach ◽  
Diffusible Signal Factor

Aim: Stenotrophomonas maltophilia ( Sm) and Burkholderia cepacia complex (BCC) are Gram-negative bacterial pathogens, which are typically multidrug resistant and excellent biofilm producers. These phenotypes are controlled by quorum sensing (QS) systems from the diffusible signal factor (DSF) family. We aim to interfere with this QS system as an alternative approach in combatting such difficult-to-treat infections. Materials & methods: A library of sulfonamide-based DSF bioisosteres was synthesized and tested against the major phenotypes regulated by QS. Results & conclusion: Several analogs display significant antibiofilm activity while the majority increase the action of the last-resort antibiotic colistin against Sm and BCC. Most compounds inhibit DSF synthesis in the Sm K279a strain. Our results support the strategy of interfering with QS communications to combat multidrug resistance.

scholarly journals Potent modulation of the CepR quorum sensing receptor and virulence in a Burkholderia cepacia complex member using non-native lactone ligands

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Betty L. Slinger ◽  
Jacqueline J. Deay ◽  
Josephine R. Chandler ◽  
Helen E. Blackwell
Keyword(s):  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
Homoserine Lactone ◽  
Communication Process ◽  
Burkholderia Cepacia Complex ◽  
Infection Model ◽  
Bacterial Communication ◽  
Human Infections ◽  
Chemical Strategies

Abstract The Burkholderia cepacia complex (Bcc) is a family of closely related bacterial pathogens that are the causative agent of deadly human infections. Virulence in Bcc species has been shown to be controlled by the CepI/CepR quorum sensing (QS) system, which is mediated by an N-acyl L-homoserine lactone (AHL) signal (C8-AHL) and its cognate LuxR-type receptor (CepR). Chemical strategies to block QS in Bcc members would represent an approach to intercept this bacterial communication process and further delineate its role in infection. In the current study, we sought to identify non-native AHLs capable of agonizing or antagonizing CepR, and thereby QS, in a Bcc member. We screened a library of AHL analogs in cell-based reporters for CepR, and identified numerous highly potent CepR agonists and antagonists. These compounds remain active in a Bcc member, B. multivorans, with one agonist 250-fold more potent than the native ligand C8-AHL, and can affect QS-controlled motility. Further, the CepR antagonists prolong C. elegans survival in an infection model. These AHL analogs are the first reported non-native molecules that both directly modulate CepR and impact QS-controlled phenotypes in a Bcc member, and represent valuable chemical tools to assess the role of QS in Bcc infections.

scholarly journals Quorum Sensing as Antivirulence Target in Cystic Fibrosis Pathogens

2019 ◽  
Vol 20 (8) ◽  
pp. 1838 ◽  
Author(s):  
Scoffone ◽  
Trespidi ◽  
Chiarelli ◽  
Barbieri ◽  
Buroni
Keyword(s):  
Cystic Fibrosis ◽  
Quorum Sensing ◽  
Burkholderia Cepacia ◽  
Autosomal Recessive ◽  
Genetic Disorder ◽  
Burkholderia Cepacia Complex ◽  
Mucus Layer ◽  
Emerging Pathogens ◽  
Alternative Pathways ◽  
Lung Infections

Cystic fibrosis (CF) is an autosomal recessive genetic disorder which leads to the secretion of a viscous mucus layer on the respiratory epithelium that facilitates colonization by various bacterial pathogens. The problem of drug resistance has been reported for all the species able to colonize the lung of CF patients, so alternative treatments are urgently needed. In this context, a valid approach is to investigate new natural and synthetic molecules for their ability to counteract alternative pathways, such as virulence regulating quorum sensing (QS). In this review we describe the pathogens most commonly associated with CF lung infections: Staphylococcus aureus, Pseudomonas aeruginosa, species of the Burkholderia cepacia complex and the emerging pathogens Stenotrophomonas maltophilia, Haemophilus influenzae and non-tuberculous Mycobacteria. For each bacterium, the QS system(s) and the molecules targeting the different components of this pathway are described. The amount of investigations published in the last five years clearly indicate the interest and the expectations on antivirulence therapy as an alternative to classical antibiotics.

scholarly journals Presence of the Hmq System and Production of 4-Hydroxy-3-Methyl-2-Alkylquinolines Are Heterogeneously Distributed between Burkholderia cepacia Complex Species and More Prevalent among Environmental than Clinical Isolates

2021 ◽  
Author(s):  
Pauline M. L. Coulon ◽  
James E. A. Zlosnik ◽  
Eric Déziel
Keyword(s):  
Quorum Sensing ◽  
Intercellular Communication ◽  
Related Species ◽  
Burkholderia Cepacia ◽  
Clinical Isolates ◽  
Burkholderia Cepacia Complex ◽  
Closely Related Species ◽  
Complex Species ◽  
Content Type ◽  
Environmental Bacteria

Environmental bacteria belonging to the various closely related species forming the Burkholderia cepacia complex (Bcc) can infect plants and animals, including humans. Their pathogenicity is regulated by intercellular communication, or quorum sensing, allowing them to collaborate instead of acting individually.

scholarly journals A Quorum-Quenching Approach To Investigate the Conservation of Quorum-Sensing-Regulated Functions within the Burkholderia cepacia Complex

2006 ◽  
Vol 72 (2) ◽  
pp. 1579-1587 ◽  
Author(s):  
Julia Wopperer ◽  
Silvia T. Cardona ◽  
Birgit Huber ◽  
Christoph A. Jacobi ◽  
Miguel A. Valvano ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Burkholderia Cepacia ◽  
Burkholderia Cepacia Complex ◽  
Phenotypic Characterization ◽  
Signal Molecules ◽  
Broad Host Range ◽  
Dependent Manner ◽  
Swarming Motility ◽  
Complex Species

ABSTRACT Taxonomic studies of the past few years have shown that the Burkholderia cepacia complex, a heterogeneous group of B. cepacia-like organisms, consists of at least nine species. B. cepacia complex strains are ubiquitously distributed in nature and have been used for biocontrol, bioremediation, and plant growth promotion purposes. At the same time, B. cepacia complex strains have emerged as important opportunistic pathogens of humans, particularly those with cystic fibrosis. All B. cepacia complex species investigated thus far use quorum-sensing (QS) systems that rely on N-acylhomoserine lactone (AHL) signal molecules to express certain functions, including the production of extracellular proteases, swarming motility, biofilm formation, and pathogenicity, in a population-density-dependent manner. In this study we constructed a broad-host-range plasmid that allowed the heterologous expression of the Bacillus sp. strain 240B1 AiiA lactonase, which hydrolyzes the lactone ring of various AHL signal molecules, in all described B. cepacia complex species. We show that expression of AiiA abolished or greatly reduced the accumulation of AHL molecules in the culture supernatants of all tested B. cepacia complex strains. Phenotypic characterization of wild-type and transgenic strains revealed that protease production, swarming motility, biofilm formation, and Caenorhabditis elegans killing efficiency was regulated by AHL in the large majority of strains investigated.

scholarly journals Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections

2020 ◽  
Vol 9 (1) ◽  
pp. 51
Author(s):  
Min Yi Wong ◽  
Yuan-Hsi Tseng ◽  
Tsung-Yu Huang ◽  
Bor-Shyh Lin ◽  
Chun-Wu Tung ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vascular Access ◽  
Burkholderia Cepacia ◽  
Bacterial Species ◽  
Diversity Indices ◽  
Burkholderia Cepacia Complex ◽  
Reca Gene ◽  
Clinical Effects ◽  
Environmental Distribution ◽  
Microbiological Characteristics

Burkholderia cepacia complex (BCC) is a group of closely related bacteria with widespread environmental distribution. BCC bacteria are opportunistic pathogens that cause nosocomial infections in patients, especially cystic fibrosis (CF). Multilocus sequence typing (MLST) is used nowadays to differentiate species within the BCC complex. This study collected 41 BCC isolates from vascular access infections (VAIs) and other clinical infections between 2014 and 2020. We preliminarily identified bacterial isolates using standard biochemical procedures and further conducted recA gene sequencing and MLST for species identification. We determined genetic diversity indices using bioinformatics software. We studied 14 isolates retrieved from patients with VAIs and observed that Burkholderia cepacia was the predominant bacterial species, and B. contaminans followed by B. cenocepacia were mainly retrieved from patients with other infections. According to MLST data, we identified that all B. contaminans isolates belonged to ST102, while a wide variety of sequence types (STs) were found in B. cenocepacia isolates. In summary, the high diversity and easy transmission of BCC increase BCC infections, which provides insights into their potential clinical effects in non-CF infections.

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