Proteomics analysis reveal that Moringa oleifera kills Escherichia coli by altering multiple biological processes

No intuito de diminuir os impactos das atividades humanas sobre o ambiente aquático, ao descarregar determinado efluente em um receptor hídrico garantindo que este esteja dentro dos parâmetros definidos pela legislação vigente, é preciso buscar tecnologias adequadas que demonstrem, principalmente, viabilidade econômica, social e ambiental para seu tratamento. As sementes da Moringa oleifera, contém agente ativo com propriedade coagulante, com capacidade de remoção de bactérias presentes na água na ordem de 90 a 99%. A técnica de usar produtos de origem vegetal permite o desenvolvimento de substâncias de tratamento que diminui ou elimina a utilização de produtos sintéticos e/ou inorgânicos, gerando, assim, menor impacto ao ecossistema e garantindo manejo sustentável do processo produtivo. Desta forma, o objetivo da pesquisa foi verificar o efeito da utilização do extrato das sementes de Moringa oleiferae do óleo essencial do Eucalyptus citriodorus em efluentes, in natura e pós-tratamento, sobre coliformes totais e Escherichia coli, existente nos efluentes de indústria de processamento de mandioca para a fabricação de amido. Utilizando amostras das lagoas de decantação, tratado com extrato aquoso de sementes de Moringa e extrato de folhas de eucaliptos em diferentes concentrações, foram realizadas diversas baterias de análises com a contagem de microrganismos de interesse. Através das análises microbiológicas, foi observado que ocorreram diferenças significativas, indicando resultados promissores, entre os efluentes com e sem tratamento, utilizando-se o extrato de sementes de Moringa oleifera e do Eucalyptus citriodorus com relação ao tratamento convencional.

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Removal of Methylene blue, Escherichia coli and Pseudomonus aeruginosa by Adsorption Process of Activated Carbon Produced from Moringa oleifera Bark

Malaysian Journal of Science Health & Technology ◽

10.33102/mjosht.v7i.105 ◽

2021 ◽

Author(s):

Md. Shahin Azad ◽

Syaza Azhari ◽

Mohd Sukri Hassan

Keyword(s):

Escherichia Coli ◽

Methylene Blue ◽

Activated Carbon ◽

Moringa Oleifera ◽

Adsorption Process ◽

Diffusion Mechanism ◽

Bacterial Load ◽

Batch Adsorption ◽

Maximum Adsorption Capacity ◽

E Coli

The utilization of biopolymer derived from Moringa oleifera bark using ZnCl2 and H2SO4 as activating agents for eliminating Methylene blue, Escherichia coli and Pseudomonas aeruginosa from producing wastewater. In this study, Methylene blue and both bacteria were effectively adsorbed by activated carbon with lowest dosage. The activated carbon was prepared from natural-by product of Moringa oleifera bark by pyrolysis in a furnace at 700°C for 1 h. The characteristics of activated carbon have been determined using Scanning Electron Microscopy (SEM), Brunauer-Emmett-Teller (BET), pHzpc (zero point charge), and FTIR spectroscopy. The obtained result were closely fitted with Freundlich isotherm model and adsorption kinetics follow the pseudo-second order model with the highest value of correlation coefficient (R2~1). Adsorption quantity was dose dependent and bacteria were maximum adsorbed using 10 mg of activated carbon as well as 25mg for methylene blue. The maximum adsorption capacity showed within 1 hour. The bacterial load was reduced by 98% for E. coli, 96% for P. aeruginosa as well as methylene blue reduced 94.2% from aqueous solution using batch adsorption methods. Adsorption process controlled by film diffusion mechanism. These result proposed that the activated carbon of Moringa oleifera can be used as a good adsorbent for the removal of Methylene blue, E. coli and P. aeruginosa.

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Moringa oleifera Seeds Extract Activity on Enteropathogenic Escherichia coli and Aeromonas hydrophyla Cells in Aquatic Microcosm

Journal of Applied Biotechnology ◽

10.5296/jab.v7i2.14917 ◽

2019 ◽

Vol 7 (2) ◽

pp. 13 ◽

Cited By ~ 1

Author(s):

Claire Stephane Metsopkeng ◽

Chretien Lontsi Djimeli ◽

Olive Vivien Noah Ewoti ◽

Lucienne Marlyse Moungang ◽

Paul Alain Nana ◽

...

Keyword(s):

Escherichia Coli ◽

Moringa Oleifera ◽

Potable Water ◽

Incubation Temperature ◽

Enteropathogenic Escherichia Coli ◽

Incubation Condition ◽

E Coli ◽

Extract Concentration ◽

Cell Incubation ◽

Potential Exploitation

This study aimed to evaluate in microcosm condition, the survival of Aeromonas hydrophila and Enteropathogenic Escherichia coli (EPEC), in the presence of M. oleifera aqueous seeds extract at concentrations varying from 1 to 40 g/L, and under 4 °C and 23 °C incubation temperature. It has been noted that cell abundances decrease gradually with the increasing in the seeds extract concentration. However, a marked cells regrowth was sometimes noted. In monospecies cell incubation condition, under 4 °C, the EPEC cells inhibition percentages (CIP) values varied from 52.12 to 99.84%. Those of A. hydrophila varied from 13.2 to 96%. The lowest CIPs were noted at the extract concentration 1g/L for EPEC and A. hydrophila. The highest CIP value was registered at 10 and 40 g/L for EPEC and at 15 g/L for A. hydrophila. Under 23 °C incubation, the EPEC CIPs values varied from 74.04 to 99.9% and those of A. hydrophila varied from 21.2 to 97.8%. For E. coli, the lowest and the highest CIP were recorded at the extract concentration 1g/L and 30 g/L, respectively. In bispecies cells incubation condition, the CIPs were relatively different. These results show the potential exploitation of M. oleifera extracts in the microbiological treatment of potable water.

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Soluble Amyloid-Beta Isoforms Predict Downstream Alzheimer’s Disease Pathology

10.21203/rs.3.rs-1032345/v1 ◽

2021 ◽

Author(s):

Guilherme Povala ◽

Bruna Bellaver ◽

Marco Antônio De Bastiani ◽

Wagner S. Brum ◽

Pamela C. L. Ferreira ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Amyloid Beta ◽

Area Under The Curve ◽

Enrichment Analysis ◽

Functional Enrichment ◽

Biological Processes ◽

Proteomics Data ◽

Proteomics Analysis ◽

Significant Enrichment

Abstract Background: Changes in soluble amyloid-beta (Aβ) levels in cerebrospinal fluid (CSF) are detectable at early preclinical stages of Alzheimer's disease (AD). However, whether Aβ levels can predict downstream AD pathological features in cognitively unimpaired (CU) individuals remains unclear. With this in mind, we aimed at investigating whether a combination of soluble Aβ isoforms can predict tau pathology (T+) and neurodegeneration (N+) positivity. Methods: We used CSF measurements of three soluble Aβ peptides (Aβ1‑38, Aβ1‑40 and Aβ1‑42) in CU individuals (n = 318) as input features in machine learning (ML) models aiming at predicting T+ and N+. Input data was used for building 2046 tuned predictive ML models with a nested cross-validation technique. Additionally, proteomics data was employed to investigate the functional enrichment of biological processes altered in T+ and N+ individuals. Results: Our findings indicate that Aβ isoforms can predict T+ and N+ with an area under the curve (AUC) of 0.929 and 0.936, respectively. Additionally, proteomics analysis identified 17 differentially expressed proteins (DEPs) in individuals wrongly classified by our ML algorithm. More specifically, enrichment analysis of gene ontology biological processes revealed an upregulation in myelinization and glucose metabolism-related processes in CU individuals wrongly predicted as T+. A significant enrichment of DEPs in pathways including biosynthesis of amino acids, glycolysis/gluconeogenesis, carbon metabolism, cell adhesion molecules and prion disease was also observed. Conclusions: Our results demonstrate that, by applying a refined ML analysis, a combination of Ab isoforms can predict T+ and N+ with a high AUC. CSF proteomics analysis highlighted a promising group of proteins that can be further explored for improving T+ and N+ prediction.

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ANÁLISE DO PERFIL ANTIBACTERIANO DO EXTRATO ETANOICO DE FLORES DE MORINGA OLEIFERA L.

10.51189/rema/1221 ◽

2021 ◽

Author(s):

Ranielly Ferreira de Sousa ◽

Josefa Rafaeli Ferreira De Sousa Rangel

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Enterococcus Faecalis ◽

Moringa Oleifera ◽

Mueller Hinton

Introdução: Moringa oleífera Lamarck, pertencente à família Moringaceae, é uma planta arbórea nativa do norte da Índia, sendo considerada uma das árvores mais úteis para os seres humanos, visto que uma variedade de aplicações nutricionais e medicinais tem sido atribuída às suas raízes, cascas, folhas, flores, frutos e sementes, dentre estás, destacam-se a sua atividade antimicrobiana. Objetivo: Desta forma, a presente pesquisa teve por objetivo avaliar o perfil antimicrobiano do extrato etanoico o de flores de Moringa oleífera L. na inibição de bactérias gram-positivas e gram-negativas. Material e métodos: Foram coletadas flores de quatro indivíduos distintos, higienizadas e submetidas a secagem em estufa (50°C), logo após ocorreu a elaboração do extrato etanoico na proporção de 100 g de flores secas e trituradas para 600 ml de etanol. Posteriormente, foram preparados quatro suspensão bacterianas com Staphylococcus aureus; Pseudomonas aeruginosa; Enterococcus faecalis e Escherichia coli., as quais, foram inoculadas em placas de Petri contendo meio de cultura ágar Mueller-Hinton. Logo após ocorreu a método de difusão em disco, com o extrato diluído na proporção de 50 mg para 1 ml de etanol. Os discos de papel com extrato foram distribuídos nos inóculos bacterianos, junto com o discos com etanol (controle negativo) e disco antibacterianos (controle positivo), a pesquisa ocorreu com triplicatas. Resultado: Como resultado, constatou que não houve a ocorrência do halo de inibição no controle negativo e no extrato, somente ao redor do disco antibacteriano (controle positivo). Conclusão: Desta forma, conclui que o extrato etanoico de flores de moringa oleífera L., preparados nestas proporções não teve ação antimicrobiana para tais bactérias.

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Evaluation of antimicrobial potential and phytochemical screening of Moringa oleifera leave extract: An Emerging folkloric medicinal plant in Maiduguri, Borno state, Nigeria

World Journal of Biology Pharmacy and Health Sciences ◽

10.30574/wjbphs.2020.4.3.0096 ◽

2020 ◽

Vol 4 (3) ◽

pp. 043-047

Author(s):

Fatima Adamu Lawan ◽

Binta Baba Shehu ◽

Ali Abba Gana Benisheikh ◽

Abba Mohammad Dige ◽

Hassan Arabi Jidda ◽

...

Keyword(s):

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Medicinal Plant ◽

Moringa Oleifera ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Moderate Activity ◽

Phytochemical Screening ◽

Antimicrobial Potential ◽

Agar Disc

The aim of this study was to evaluate the antimicrobial potential and phytochemical screening of Moringa oleifera leave extract as folkloric medicinal alternative. The phytochemical screening result revealed the presence of Alkanoids, Saponins, Fats & oil, Terpenoids and Tanin in all the solvents used for the study. Agar-disc diffusion method were used to determine the antimicrobial activities of the Moringa oleifera extracts on microorganisms. Maximum activities of inhibition were recorded on Ethyl acetate (50mg/ml) against Pseudomonas auroginusa, moderarate activities against Escherichia coli. Likewise 50mg/ml of Haxane extract show weak activities on Pseudomonas aeruginosa, moderate in both Staphalococcus aureus and Escherichia coli. Whereas 50mg/ml of Methanol show moderate activity on Pseudomonas aeruginosa, Staphalococcus aureus and weak activities on Escherichia coli respectively. The research revealed that Moringa oleifera bioactive components would be a safer alternative for curative and preventive emerging folkloric medicinal plant for treatment of bacterial ailments in Maiduguri, Borno state, Nigeria.

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Cryo-EM reveals unique structural features of the FhuCDB Escherichia coli ferrichrome importer

Communications Biology ◽

10.1038/s42003-021-02916-2 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Wenxin Hu ◽

Hongjin Zheng

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Molecular Basis ◽

Iron Uptake ◽

Structural Features ◽

Biological Processes ◽

Type Ii ◽

Functional Studies ◽

Cryo Electron Microscopy ◽

Translocation Pathway

AbstractAs one of the most elegant biological processes developed in bacteria, the siderophore-mediated iron uptake demands the action of specific ATP-binding cassette (ABC) importers. Although extensive studies have been done on various ABC importers, the molecular basis of these iron-chelated-siderophore importers are still not fully understood. Here, we report the structure of a ferrichrome importer FhuCDB from Escherichia coli at 3.4 Å resolution determined by cryo electron microscopy. The structure revealed a monomeric membrane subunit of FhuB with a substrate translocation pathway in the middle. In the pathway, there were unique arrangements of residues, especially layers of methionines. Important residues found in the structure were interrogated by mutagenesis and functional studies. Surprisingly, the importer’s ATPase activity was decreased upon FhuD binding, which deviated from the current understanding about bacterial ABC importers. In summary, to the best of our knowledge, these studies not only reveal a new structural twist in the type II ABC importer subfamily, but also provide biological insights in the transport of iron-chelated siderophores.

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Anti-Bacterial from Moringa oleifera Lamk Crude Extracts

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1060.223 ◽

2014 ◽

Vol 1060 ◽

pp. 223-226

Author(s):

Perayot Pamonsinlapatham

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Moringa Oleifera ◽

Soxhlet Extraction ◽

Stem Bark ◽

Bacterial Activity ◽

Diffusion Method ◽

Crude Extracts ◽

Agar Diffusion Method

Moringa oleiferaLamk (Moringaceae) is a worldwide use for food and traditional medicine. In this study, plants were separated into seven parts: 1) Leaf, 2) Drum, 3) Branching, 4) Seed, 5) Outer stem, 6) Stem bark, and 7) Root, respectively. Then, they were extracted by two different methods: methanol soxhlet extraction and ethanol maceration. All crude extracts were tested for anti-bacterial activity by agar diffusion method and colorimetric micro-dilution plate technique. The results showed that 7 parts of Moringa crude extracts had low anti-bacterial activity againstStaphylococcus aureus,Escherichia coli, andPseudomonas aeruginosawhen compared with standard antibiotic (ampicillin or gentamycin). In conclusion, anti-bacterial effects ofM. oleiferacrude extracts had low anti-bacterial activityin vitrotest.

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