Seed priming with cypress leaf extract enhances photosynthesis and antioxidative defense in zucchini seedlings under salt stress

Currently, seed priming is reported as an efficient and low-cost approach to increase crop yield, which could not only promote seed germination and improve plant growth state but also increase abiotic stress tolerance. Salinity represents one of the most significant abiotic stresses that alters multiple processes in plants. The accumulation of polyamines (PAs) in response to salt stress is one of the most remarkable plant metabolic responses. This paper examined the effect of osmopriming on endogenous polyamine metabolism at the germination and early seedling development of Brassica napus in relation to salinity tolerance. Free, conjugated and bound polyamines were analyzed, and changes in their accumulation were discussed with literature data. The most remarkable differences between the corresponding osmoprimed and unprimed seeds were visible in the free (spermine) and conjugated (putrescine, spermidine) fractions. The arginine decarboxylase pathway seems to be responsible for the accumulation of PAs in primed seeds. The obvious impact of seed priming on tyramine accumulation was also demonstrated. Moreover, the level of ethylene increased considerably in seedlings issued from primed seeds exposed to salt stress. It can be concluded that the polyamines are involved in creating the beneficial effect of osmopriming on germination and early growth of Brassica napus seedlings under saline conditions through moderate changes in their biosynthesis and accumulation.

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The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽

10.1371/journal.pone.0245505 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0245505

Author(s):

Xiaofei Chen ◽

Ruidong Zhang ◽

Yifan Xing ◽

Bing Jiang ◽

Bang Li ◽

...

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Germination Rate ◽

Principal Component ◽

Dry Weight ◽

Seed Priming ◽

Nacl Stress ◽

Future Research ◽

Fresh Weight ◽

Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

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Seed Priming and Seedling Pre-treatment Induced Tolerance to Drought and Salt Stress: Recent Advances

Priming and Pretreatment of Seeds and Seedlings ◽

10.1007/978-981-13-8625-1_12 ◽

2019 ◽

pp. 253-263 ◽

Cited By ~ 2

Author(s):

Smita Sahoo ◽

Pankaj Borgohain ◽

Bedabrata Saha ◽

Debojyoti Moulick ◽

Bhaben Tanti ◽

...

Keyword(s):

Salt Stress ◽

Seed Priming ◽

Recent Advances ◽

Drought And Salt Stress ◽

Pre Treatment ◽

Induced Tolerance

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Mechanisms of Seed Priming Involved in Salt Stress Amelioration

Priming and Pretreatment of Seeds and Seedlings ◽

10.1007/978-981-13-8625-1_11 ◽

2019 ◽

pp. 219-251 ◽

Cited By ~ 3

Author(s):

Magdi T. Abdelhamid ◽

Raafat R. El-Masry ◽

Darwish S. Darwish ◽

Mazhar M. F. Abdalla ◽

Shinya Oba ◽

...

Keyword(s):

Salt Stress ◽

Seed Priming ◽

Stress Amelioration

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Seed Priming-Mediated Improvement of Plant Morphophysiology Under Salt Stress

Priming and Pretreatment of Seeds and Seedlings ◽

10.1007/978-981-13-8625-1_10 ◽

2019 ◽

pp. 205-217

Author(s):

Abdul Rehman ◽

Babar Shahzad ◽

Aman Ullah ◽

Faisal Nadeem ◽

Mohsin Tanveer ◽

...

Keyword(s):

Salt Stress ◽

Seed Priming

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Alleviation of salt stress using gibberellic acid in Chinese cabbage

Acta Agronomica Hungarica ◽

10.1556/aagr.60.2012.4.5 ◽

2012 ◽

Vol 60 (4) ◽

pp. 345-355 ◽

Cited By ~ 2

Author(s):

M. Jamil ◽

M. Ashraf ◽

E. Rha

Keyword(s):

Salt Stress ◽

Gibberellic Acid ◽

Chinese Cabbage ◽

Germination Rate ◽

Harmful Effect ◽

Seed Priming ◽

Nacl Stress ◽

Growth And Yield ◽

Distilled Water ◽

Membrane Injury

Salinity reduces plant growth and yield by affecting morphological and physiological processes. To alleviate the harmful effects of salt stress various approaches involving plant hormones are used. In this study several parameters involving the measurement of cell membrane injury were used to observe whether stress tolerance could be enhanced in Chinese cabbage (B. oleracea capitata L. Chinensis group) by soaking the seeds for 10 h in distilled water (control), or in 100, 150 or 200 mg l−1 gibberellic acid (GA3). The NaCl concentrations were 0 (control), 50, 100 and 150 mM. Seed treated with GA3 showed increased water uptake and decreased electrolyte leakage as compared to that of distilled water-primed seeds even 24 h after soaking under control conditions. Seed priming with GA3 increased the final germination and the germination rate (1/t50, where t50 is the time to 50% germination) under salt stress conditions. Seed priming also alleviated the harmful effect of salt stress on cabbage in terms of fresh and dry weights. Leaf area was higher in plants raised from seeds primed with the higher GA3 concentrations as compared with those raised from seeds treated with distilled water under control conditions (without NaCl) or at 50 mM NaCl stress. The chlorophyll content increased with the NaCl concentration, especially in plants grown from seeds primed with GA3. Plants grown from GA3-primed seeds also suffered lower cellular injury both under control conditions and under NaCl stress.

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Low Doses of Cuscuta reflexa Extract Act as Natural Biostimulants to Improve the Germination Vigor, Growth, and Grain Yield of Wheat Grown under Water Stress: Photosynthetic Pigments, Antioxidative Defense Mechanisms, and Nutrient Acquisition

Biomolecules ◽

10.3390/biom10091212 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1212 ◽

Cited By ~ 2

Author(s):

Qasim Ali ◽

Rashida Perveen ◽

Mohamed A. El-Esawi ◽

Shafaqat Ali ◽

Syed Makhdoom Hussain ◽

...

Keyword(s):

Water Stress ◽

Seed Germination ◽

Seed Yield ◽

Photosynthetic Pigments ◽

Defense Mechanisms ◽

Seed Priming ◽

Low Doses ◽

Antioxidative Defense ◽

Cuscuta Reflexa ◽

Germination Vigor

The present study was conducted to investigate the effects of Cuscuta reflexa extract (CRE) on the activities of germination enzymes, seed germination vigor, biomass production, physio-biochemical attributes, and seed yield of water-stressed wheat plants. Different levels of CRE (0, 10, 20, 30, 40, and 50%), including water soaking, were used as seed priming. Water stress negatively affected the seed germination, germination enzyme activities, growth, yield, and different physio-biochemical attributes of wheat plants. Low doses of CRE (10, 20, and 30%) ameliorated the adverse effects of water stress on seed germination attributes, and activities of germination enzymes, but negative impacts were recorded at higher doses (40 and 50%) of CRE. Water-stressed wheat plants grown from seeds pre-treated with low doses of CRE also showed better growth and yield as compared with non-treated ones, and that was associated with an improvement in water relations, photosynthetic pigments, nutrient acquisition, reduced lipid peroxidation, and better antioxidative defense mechanisms. The maximum increase in seed yield was 14.77 and 12.32%, found in plants grown from seeds treated with 20% and 10% CRE, respectively. In conclusion, it is suggested that using low doses of CRE as seed priming can contribute to better wheat yield under water stress, especially in semi-arid and arid areas.

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