Seed-priming with H2O2 alleviates subsequent salt stress by preventing ROS production and amplifying antioxidant defense in cauliflower seeds and seedlings

Currently, seed priming is reported as an efficient and low-cost approach to increase crop yield, which could not only promote seed germination and improve plant growth state but also increase abiotic stress tolerance. Salinity represents one of the most significant abiotic stresses that alters multiple processes in plants. The accumulation of polyamines (PAs) in response to salt stress is one of the most remarkable plant metabolic responses. This paper examined the effect of osmopriming on endogenous polyamine metabolism at the germination and early seedling development of Brassica napus in relation to salinity tolerance. Free, conjugated and bound polyamines were analyzed, and changes in their accumulation were discussed with literature data. The most remarkable differences between the corresponding osmoprimed and unprimed seeds were visible in the free (spermine) and conjugated (putrescine, spermidine) fractions. The arginine decarboxylase pathway seems to be responsible for the accumulation of PAs in primed seeds. The obvious impact of seed priming on tyramine accumulation was also demonstrated. Moreover, the level of ethylene increased considerably in seedlings issued from primed seeds exposed to salt stress. It can be concluded that the polyamines are involved in creating the beneficial effect of osmopriming on germination and early growth of Brassica napus seedlings under saline conditions through moderate changes in their biosynthesis and accumulation.

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The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽

10.1371/journal.pone.0245505 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0245505

Author(s):

Xiaofei Chen ◽

Ruidong Zhang ◽

Yifan Xing ◽

Bing Jiang ◽

Bang Li ◽

...

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Germination Rate ◽

Principal Component ◽

Dry Weight ◽

Seed Priming ◽

Nacl Stress ◽

Future Research ◽

Fresh Weight ◽

Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

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Supplemental Selenium and Boron Mitigate Salt-Induced Oxidative Damages in Glycine max L.

Plants ◽

10.3390/plants10102224 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2224

Author(s):

Mira Rahman ◽

Khussboo Rahman ◽

Khadeja Sultana Sathi ◽

Md. Mahabub Alam ◽

Kamrun Nahar ◽

...

Keyword(s):

Oxidative Stress ◽

Salt Stress ◽

Antioxidant Defense ◽

Foliar Application ◽

Growth Parameters ◽

Monodehydroascorbate Reductase ◽

Ros Scavenging ◽

H2o2 Content ◽

Mda Content ◽

Different Levels

The present investigation was executed with an aim to evaluate the role of exogenous selenium (Se) and boron (B) in mitigating different levels of salt stress by enhancing the reactive oxygen species (ROS) scavenging, antioxidant defense and glyoxalase systems in soybean. Plants were treated with 0, 150, 300 and 450 mM NaCl at 20 days after sowing (DAS). Foliar application of Se (50 µM Na2SeO4) and B (1 mM H3BO3) was accomplished individually and in combined (Se+B) at three-day intervals, at 16, 20, 24 and 28 DAS under non-saline and saline conditions. Salt stress adversely affected the growth parameters. In salt-treated plants, proline content and oxidative stress indicators such as malondialdehyde (MDA) content and hydrogen peroxide (H2O2) content were increased with the increment of salt concentration but the relative water content decreased. Due to salt stress catalase (CAT), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glyoxalase I (Gly I) and glyoxalase II (Gly II) activity decreased. However, the activity of ascorbate peroxidase (APX), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione S-transferase (GST) and peroxidase (POD) increased under salt stress. On the contrary, supplementation of Se, B and Se+B enhanced the activities of APX, MDHAR, DHAR, GR, CAT, GPX, GST, POD, Gly I and Gly II which consequently diminished the H2O2 content and MDA content under salt stress, and also improved the growth parameters. The results reflected that exogenous Se, B and Se+B enhanced the enzymatic activity of the antioxidant defense system as well as the glyoxalase systems under different levels of salt stress, ultimately alleviated the salt-induced oxidative stress, among them Se+B was more effective than a single treatment.

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A rice mutant defective in antioxidant-defense system and sodium homeostasis possesses increased sensitivity to salt stress

Biologia Plantarum ◽

10.1007/s10535-015-0561-7 ◽

2016 ◽

Vol 60 (1) ◽

pp. 86-94 ◽

Cited By ~ 8

Author(s):

K. -C. Lin ◽

W. -S. Jwo ◽

N. N. P. Chandrika ◽

T. -M. Wu ◽

M. -H. Lai ◽

...

Keyword(s):

Salt Stress ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Defense System ◽

Sodium Homeostasis ◽

Rice Mutant ◽

Increased Sensitivity

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Influence of Salt Stress on Different Pepper Genotypes: Ion Homeostasis, Antioxidant Defense, and Secondary Metabolites

Global Journal Of Botanical Science ◽

10.12974/2311-858x.2021.09.3 ◽

2021 ◽

Vol 9 (1) ◽

pp. 14-20

Author(s):

Sebnem Kusvuran ◽

◽

Sevinc Kiran ◽

Ozlem Altuntas

Keyword(s):

Salt Stress ◽

Secondary Metabolites ◽

Antioxidant Defense ◽

Ion Homeostasis

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Seed priming with cypress leaf extract enhances photosynthesis and antioxidative defense in zucchini seedlings under salt stress

Scientia Horticulturae ◽

10.1016/j.scienta.2021.110707 ◽

2022 ◽

Vol 293 ◽

pp. 110707

Author(s):

Abdelaleim Ismail ElSayed ◽

Mohammed Suhail Rafudeen ◽

Showkat Ahmad Ganie ◽

M Sazzad Hossain ◽

Ayman M. Gomaa

Keyword(s):

Salt Stress ◽

Leaf Extract ◽

Seed Priming ◽

Antioxidative Defense

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Seed Priming and Seedling Pre-treatment Induced Tolerance to Drought and Salt Stress: Recent Advances

Priming and Pretreatment of Seeds and Seedlings ◽

10.1007/978-981-13-8625-1_12 ◽

2019 ◽

pp. 253-263 ◽

Cited By ~ 2

Author(s):

Smita Sahoo ◽

Pankaj Borgohain ◽

Bedabrata Saha ◽

Debojyoti Moulick ◽

Bhaben Tanti ◽

...

Keyword(s):

Salt Stress ◽

Seed Priming ◽

Recent Advances ◽

Drought And Salt Stress ◽

Pre Treatment ◽

Induced Tolerance

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Mechanisms of Seed Priming Involved in Salt Stress Amelioration

Priming and Pretreatment of Seeds and Seedlings ◽

10.1007/978-981-13-8625-1_11 ◽

2019 ◽

pp. 219-251 ◽

Cited By ~ 3

Author(s):

Magdi T. Abdelhamid ◽

Raafat R. El-Masry ◽

Darwish S. Darwish ◽

Mazhar M. F. Abdalla ◽

Shinya Oba ◽

...

Keyword(s):

Salt Stress ◽

Seed Priming ◽

Stress Amelioration

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Seed Priming-Mediated Improvement of Plant Morphophysiology Under Salt Stress

Priming and Pretreatment of Seeds and Seedlings ◽

10.1007/978-981-13-8625-1_10 ◽

2019 ◽

pp. 205-217

Author(s):

Abdul Rehman ◽

Babar Shahzad ◽

Aman Ullah ◽

Faisal Nadeem ◽

Mohsin Tanveer ◽

...

Keyword(s):

Salt Stress ◽

Seed Priming

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Exogenous Tebuconazole and Trifloxystrobin Regulates Reactive Oxygen Species Metabolism Toward Mitigating Salt-Induced Damages in Cucumber Seedling

Plants ◽

10.3390/plants8100428 ◽

2019 ◽

Vol 8 (10) ◽

pp. 428 ◽

Cited By ~ 5

Author(s):

Sayed Mohsin ◽

Mirza Hasanuzzaman ◽

M. Bhuyan ◽

Khursheda Parvin ◽

Masayuki Fujita

Keyword(s):

Reactive Oxygen Species ◽

Salt Stress ◽

Antioxidant Defense ◽

Ion Homeostasis ◽

Reactive Oxygen ◽

Cucumber Plant ◽

Enzymatic Antioxidants ◽

Oxygen Species ◽

Exogenous Application ◽

Antioxidant Defense Systems

The present study investigated the role of tebuconazole (TEB) and trifloxystrobin (TRI) on cucumber plants (Cucumis sativus L. cv. Tokiwa) under salt stress (60 mM NaCl). The cucumber plants were grown semi-hydroponically in a glasshouse. Plants were exposed to two different doses of fungicides (1.375 µM TEB + 0.5 µM TRI and 2.75 µM TEB + 1.0 µM TRI) solely and in combination with NaCl (60 mM) for six days. The application of salt phenotypically deteriorated the cucumber plant growth that caused yellowing of the whole plant and significantly destructed the contents of chlorophyll and carotenoids. The oxidative damage was created under salinity by increasing the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2), and electrolytic leakage (EL) resulting in the disruption of the antioxidant defense system. Furthermore, in the leaves, stems, and roots of cucumber plants increased Na+ content was observed under salt stress, whereas the K+/Na+ ratio and contents of K+, Ca2+, and Mg2+ decreased. In contrast, the exogenous application of TEB and TRI reduced the contents of MDA, H2O2, and EL by improving the activities of enzymatic and non-enzymatic antioxidants. In addition, ion homeostasis was regulated by reducing Na+ uptake and enhanced K+ accumulation and the K+/Na+ ratio after application of TEB and TRI. Therefore, this study indicates that the exogenous application of TEB and TRI enhanced salt tolerance in cucumber plants by regulating reactive oxygen species production and antioxidant defense systems.

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