scholarly journals Assessing the influence of the carbon source on the abatement of industrial N 2 O emissions coupled with the synthesis of added-value bioproducts

2017 ◽  
Vol 598 ◽  
pp. 765-771 ◽  
Author(s):  
Osvaldo D. Frutos ◽  
Gonzalo Barriguín ◽  
Raquel Lebrero ◽  
Raúl Muñoz
Keyword(s):  
Carbon Source ◽  
Added Value

scholarly journals Production of Omega-3 Oil by Aurantiochytrium mangrovei Using Spent Osmotic Solution from Candied Fruit Industry as Sole Organic Carbon Source

Processes ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1834
Author(s):  
Giovanni L. Russo ◽  
Antonio L. Langellotti ◽  
Thierry Blasco ◽  
Maria Oliviero ◽  
Raffaele Sacchi ◽  
...  
Keyword(s):  
Carbon Source ◽  
Organic Carbon ◽  
Osmotic Dehydration ◽  
Sugar Content ◽  
Scale Up ◽  
Liquid Waste ◽  
Added Value ◽  
Omega 3 ◽  
Osmotic Solution ◽  
Organic Carbon Source

Osmotic dehydration is an important phase in the production of dried products, including most fruits and vegetables, in the food industry. The drying process for candied fruit produces a liquid waste called “spent osmotic solution”, which is characterized by a high content of organic compounds, mostly dissolved sugars. The sugar content of this food by-product could be valorized through the growth of biomass with a high added value. In this study, the spent osmotic solution from the candied fruit industry was used as an organic carbon source for the growth and production of docosahexaenoic acid (DHA) in the cultivation of Aurantiochytrium mangrovei RCC893. The carbon content of the standard media was completely replaced by the sugars present in this food by-product. After that, the growth condition of this strain was optimized through response surface methodologies using a central composite design (CCD), and the optimal combination of the spent osmotic solution and nitrogen was established. Moreover, a scale-up trial was performed using the optimal conditions obtained after CCD to evaluate the scalability of the process.

scholarly journals The production, molecular weight and viscosifying power of alginate produced by Azotobacter vinelandii is affected by the carbon source in submerged cultures

DYNA ◽  
2015 ◽  
Vol 82 (194) ◽  
pp. 21-26 ◽  
Author(s):  
Mauricio A. Trujillo-Roldán ◽  
John Fredy Monsalve-Gil ◽  
Angélica Maria Cuesta-Álvarez ◽  
Norma A. Valdez-Cruz
Keyword(s):  
Molecular Weight ◽  
Carbon Source ◽  
Biomass Concentration ◽  
Sugarcane Juice ◽  
Added Value ◽  
Specific Productivity ◽  
Molecular Characteristics ◽  
Submerged Cultures ◽  
Alginate Production ◽  
The Impact

Alginate is a linear polymer composed of <span style="font-family: symbol;">b</span>-1,4 linked mannuronic acid and its epimer, <span style="font-family: symbol;">a</span>-L-guluronic acid, and frequently extracted from marine algae, as from bacteria such as Azotobacter and Pseudomonas. Here, we show the impact of conventional and unconventional carbon sources on A. vinelandii growth, alginate production, its mean molecular weight (MMW) and its viscosifying power. Starting with 20 g/L of sugars, the highest biomass concentration was obtained using deproteinized and hydrolyzed whey (6.67±0.72 g/L), and sugarcane juice (6.68±0.45 g/L). However, the maximum alginate production was achieved using sucrose (5.11±0.37 g/L), as well the highest alginate yield and specific productivity. Otherwise, the higher alginate MMW was obtained using sugarcane juice (1203±120 kDa), and the higher viscosifying power was obtained using deproteinized/ hydrolyzed whey (23.8±2.6 cps L/galg). This information suggests that it is possible to manipulate the productivity and molecular characteristics of alginates, as a function of the carbon source used. All this, together with the knowledge of the effects of environmental conditions will allow for high yields of high added value biopolymers.

scholarly journals Potential production of bioplastics polyhydroxyalkanoates using residual glycerol

2018 ◽  
Vol 3 (1) ◽  
pp. 055-060 ◽  
Author(s):  
Priscilla Barbosa Sales de Albuquerque ◽  
Katarynna Santos de Araújo ◽  
Kelvin Augusto Azevedo da Silva ◽  
Laureen Michelle Houllou ◽  
Gabriel Olivo Locatelli ◽  
...  
Keyword(s):  
Carbon Source ◽  
Shake Flask ◽  
Carbon Sources ◽  
Added Value ◽  
Efficient Production ◽  
Substrate Consumption ◽  
Potential Production ◽  
Alternative Product ◽  
Pha Production ◽  
Biodiesel Industry

Cupriavidus necator was used for polyhydroxyalkanoate (PHA) production with the aim to compare the substrate consumption between pure glycerol (PG) and the glycerol obtained from biodiesel industry (GB). Shake flask experiments were performed with 20 g/L for both the carbon sources, incubated in a shaking incubator at 35 ºC for up to 72 h. At the end, the residual carbon source was analyzed by HPLC, the biomass recuperation for biopolymer extraction and biopolymer characterized by FTIR. The results demonstrated that 35.75 % of PG was consumed during biomass production, while 45.08 % was the value consumed for GB, which can indicate the higher PHA accumulation in GB-sample, as observed by microscopy analyses. Fourier Transform Infrared (FTIR) spectroscopy was performed to confirm the PHA nature of PG and GB samples, and confirmed the establishment of more hydrogen bonds in the PG one, suggesting the surplus of glycerol in the obtained-biopolymer. Since it is interesting the utilization of GB for obtaining added-value products along with biodiesel, this study reported the efficient production of PHA by C. necator using GB as carbon source and its promising use in the bioplastic industry as an alternative product for petrochemical plastics, foreseeing the improvement of a sustainable industry based on biofuels and biopolymers.

scholarly journals Biotransformation of 2,4-dinitrotoluene in a phototrophic co-culture of engineeredSynechococcus elongatusandPseudomonas putida

2018 ◽  
Author(s):  
Derek T. Fedeson ◽  
Pia Saake ◽  
Patricia Calero ◽  
Pablo Iván Nikel ◽  
Daniel C. Ducat
Keyword(s):  
Carbon Source ◽  
Division Of Labor ◽  
Heterotrophic Bacterium ◽  
Bacterial Species ◽  
Agricultural Practices ◽  
Catalytic Efficiency ◽  
Environmental Pollutant ◽  
Added Value ◽  
Production Platform ◽  
Specialist Species

AbstractIn contrast to the current paradigm of using microbial monocultures in most biotechnological applications, increasing efforts are being directed towards engineering mixed-species consortia to perform functions that are difficult to program into individual strains. Additionally, the division of labor between specialist species found in natural consortia can lead to increased catalytic efficiency and stability relative to a monoculture or a community composed of generalists. In this work, we have designed a synthetic co-culture for phototrophic degradation of xenobiotics, composed of a cyanobacterium, (Synechococcus elongatusPCC 7942) and a heterotrophic bacterium (Pseudomonas putidaEM173). Cyanobacteria fix CO2through photosynthetic metabolism and secrete sufficient carbohydrates to support the growth and active metabolism ofP. putida, which has been engineered to consume sucrose as the only carbon source and to degrade the environmental pollutant 2,4-dinitrotoluene (2,4-DNT). The synthetic consortium is able to degrade 2,4-DNT with only light and CO2as inputs for the system, and it was stable over time through repeated backdilutions. Furthermore, cycling this consortium through low nitrogen medium promoted the accumulation of polyhydroxyalkanoate (PHA)–an added-value biopolymer–inP. putida, thus highlighting the versatility of this production platform. Altogether, the synthetic consortium allows for simultaneous bioproduction of PHA and remediation of the industrial pollutant 2,4-DNT, using light and CO2as inputs.ImportanceIn this study, we have created an artificial consortium composed of two bacterial species that enables the degradation of the industrially-produced environmental pollutant 2,4-DNT while simultaneously producing PHA bioplastic. In these co-cultures, the photosynthetic cyanobacteria fuel an engineeredP. putidastrain programmed both to use sucrose as a carbon source and to perform the biotransformation of 2,4-DNT. The division of labor in this synthetic co-culture is reminiscent of that commonly observed in microbial communities and represents a proof-of-principle example of how artificial consortia can be employed for bioremediation purposes. Furthermore, this co-culture system enabled the utilization of freshwater sources that could not be utilized in classical agriculture settings, reducing the potential competition of this alternative method of bioproduction with current agricultural practices, as well as remediation of contaminated water streams.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

The Added Value of Graphical Input and Display for Electron Lens Design

1990 ◽  
Vol 48 (1) ◽  
pp. 190-191
Author(s):  
B. Lencova ◽  
G. Wisselink
Keyword(s):  
Flux Density ◽  
Numerical Data ◽  
Added Value ◽  
Lens Design ◽  
Step Size ◽  
Magnetic Lens ◽  
Flux Lines ◽  
Fine Mesh ◽  
Electron Lens ◽  
User Friendly

Recent progress in computer technology enables the calculation of lens fields and focal properties on commonly available computers such as IBM ATs. If we add to this the use of graphics, we greatly increase the applicability of design programs for electron lenses. Most programs for field computation are based on the finite element method (FEM). They are written in Fortran 77, so that they are easily transferred from PCs to larger machines.The design process has recently been made significantly more user friendly by adding input programs written in Turbo Pascal, which allows a flexible implementation of computer graphics. The input programs have not only menu driven input and modification of numerical data, but also graphics editing of the data. The input programs create files which are subsequently read by the Fortran programs. From the main menu of our magnetic lens design program, further options are chosen by using function keys or numbers. Some options (lens initialization and setting, fine mesh, current densities, etc.) open other menus where computation parameters can be set or numerical data can be entered with the help of a simple line editor. The "draw lens" option enables graphical editing of the mesh - see fig. I. The geometry of the electron lens is specified in terms of coordinates and indices of a coarse quadrilateral mesh. In this mesh, the fine mesh with smoothly changing step size is calculated by an automeshing procedure. The options shown in fig. 1 allow modification of the number of coarse mesh lines, change of coordinates of mesh points or lines, and specification of lens parts. Interactive and graphical modification of the fine mesh can be called from the fine mesh menu. Finally, the lens computation can be called. Our FEM program allows up to 8000 mesh points on an AT computer. Another menu allows the display of computed results stored in output files and graphical display of axial flux density, flux density in magnetic parts, and the flux lines in magnetic lenses - see fig. 2. A series of several lens excitations with user specified or default magnetization curves can be calculated and displayed in one session.

Preliminary Findings on the Use of Session Questionnaires to Improve Clinician-Client Alliance With Special Consideration to Clinical Education

2015 ◽  
Vol 25 (1) ◽  
pp. 50-60
Author(s):  
Anu Subramanian
Keyword(s):  
Graduate Students ◽  
Clinical Education ◽  
Clinical Decision Making ◽  
Rating Scale ◽  
Treatment Effectiveness ◽  
Clinical Decision ◽  
Added Value ◽  
Therapeutic Outcomes ◽  
Specific Feedback ◽  
Stakeholder Perspective

ASHA's focus on evidence-based practice (EBP) includes the family/stakeholder perspective as an important tenet in clinical decision making. The common factors model for treatment effectiveness postulates that clinician-client alliance positively impacts therapeutic outcomes and may be the most important factor for success. One strategy to improve alliance between a client and clinician is the use of outcome questionnaires. In the current study, eight parents of toddlers who attended therapy sessions at a university clinic responded to a session outcome questionnaire that included both rating scale and descriptive questions. Six graduate students completed a survey that included a question about the utility of the questionnaire. Results indicated that the descriptive questions added value and information compared to using only the rating scale. The students were varied in their responses regarding the effectiveness of the questionnaire to increase their comfort with parents. Information gathered from the questionnaire allowed for specific feedback to graduate students to change behaviors and created opportunities for general discussions regarding effective therapy techniques. In addition, the responses generated conversations between the client and clinician focused on clients' concerns. Involving the stakeholder in identifying both effective and ineffective aspects of therapy has advantages for clinical practice and education.

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