Development of a demineralized and decellularized human epiphyseal bone scaffold for tissue engineering: A histological study

2018 ◽  
Vol 55 ◽  
pp. 46-52 ◽  
Author(s):  
Elham Abedin ◽  
Roya Lari ◽  
Nasser Mahdavi Shahri ◽  
Masoud Fereidoni
Keyword(s):  
Tissue Engineering ◽  
Histological Study ◽  
Bone Scaffold ◽  
Epiphyseal Bone

Bone Scaffold Fabrication System Study

2007 ◽  
Author(s):  
Lin Lu ◽  
David Wootton ◽  
Peter I. Lelkes ◽  
Jack Zhou
Keyword(s):  
Tissue Engineering ◽  
Flow Rate ◽  
Intelligent System ◽  
Bone Substitutes ◽  
Water Soluble ◽  
Health Concern ◽  
Bone Scaffold ◽  
Systems And Control ◽  
Porous Biomaterials ◽  
And Control

Musculoskeletal conditions are a major health concern in United States because of a large aging population and increased occurrence of sport-related injuries. The need for bone substitutes is especially important. Traditional treatments of bone-defect have many limitations. Bone tissue engineering may offer a less painful alternative to traditional bone grafts with lower risk of infection. This research integrates biomimetic modeling, solid freedom fabrication (SFF), systems and control, and tissue engineering in one intelligent system for structured, highly porous biomaterials, which will be applied to bone scaffolds. Recently a new SFF-based fabrication system has been developed, which uses a pressurized extrusion to print highly biocompatible and water soluble sucrose bone scaffold porogens. The fabrication process for PCL scaffold implemented and tested using the newly developed porogen system. The resultant scaffold demonstrates the defined porous structure designed into the sucrose porogens. The viscosity of sucrose mixture has been tested and analyzed. The flow rate measurements of sucrose machine have been carried out. The input factor, which induced uncertainty in the flow rate of the microprinting system has been analyzed. The result showed that the reservoir pressure was dominant to determine the flow rate. This is very important for improving the quality control of our fabrication system.

Stem cell recruitment based on scaffold features for bone tissue engineering

2021 ◽  
Author(s):  
Bin Xia ◽  
Yaxin Deng ◽  
Yonggang Lv ◽  
Guobao Chen
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Bone Formation ◽  
Chemical Modification ◽  
Bone Tissue Engineering ◽  
Bone Scaffold ◽  
New Bone Formation ◽  
Cell Recruitment ◽  
Physical And Chemical

Proper physical and chemical modification of a bone scaffold can effectively recruit endogenous stem cells to participate in the new bone formation.

Progress in polymer nanocomposites for bone regeneration and engineering

2020 ◽  
pp. 096739112091365 ◽  
Author(s):  
Christopher Igwe Idumah
Keyword(s):  
Tissue Engineering ◽  
Mechanical Behavior ◽  
Bone Regeneration ◽  
Polymer Nanocomposites ◽  
Metallic Nanoparticles ◽  
Bone Repair ◽  
Fiber Composites ◽  
Bone Scaffold ◽  
Medical Field ◽  
The Matrix

The ultimate aim of tissue engineering entails fabrication of functional replacements for damaged organs or tissues. Scaffolds facilitate the proliferation of cells, while also improving their various functions. Scaffolds are 3-D structures capable of imitating mechanical and bioactive behaviors of tissues extracellular matrix, which provides enabling environment for cellular bonding, proliferation, and distinction. Hence, scaffolds are often applied in tissue engineering with the aim of facilitating damaged tissue regeneration which is a very important aspect of bone repair. Polymers are broadly utilized in tissue engineering due to their inherent versatility. However, polymers cannot attain mechanical behavior comparable to the bone. Thus, polymer nanocomposites fabricated through inclusion of fibers/or uniformly distributed ceramic/metallic nanoparticles in the matrix are potential materials for bone scaffold fabrication because inclusion of fiber or nanoparticles enhances composites mechanical behavior, while also improving other properties. Hence, this article elucidates recent trailblazing studies in polymer fiber composites and nanocomposites applied in the medical field especially in tissue engineering and bone regeneration. Also insights into market prospects and forecasts are presented.

Histological study of an experimentally constructed decellularized rat lung as a model of whole organ three-dimensional natural scaffold

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Marium Romany Abdelsayed ◽  
Suzi Sobhy Atalla ◽  
Gehan Khalaf Megahed ◽  
Asmaa Abd El-Monem Abo Zeid
Keyword(s):  
Tissue Engineering ◽  
Histological Study ◽  
Three Dimensional ◽  
Nuclear Material ◽  
Biological Properties ◽  
Basement Membranes ◽  
Elastic Fibers ◽  
Scientific Exploration ◽  
And Migration ◽  
End Stage

Abstract Introduction With the increase of end stage lung diseases and the great problems facing lung transplantation tissue engineering become a promising solution. The first step in lung engineering is to obtain a 3D Extracellular matrix lung scaffold via decellularization. Decellularization aims to remove cells from tissue ultrastructure while preserving the mechanical and biological properties of the tissue. Intact ECM provides critical cues for differentiation and migration of cells that are seeded onto the organ scaffold. Objectives This study aimed to obtain an intact and well-preserved ECM lung scaffold by decellularization of rat lungs. Methods Decellularization of lungs of ten Wistar rats was achieved by perfusing detergents through the pulmonary artery. The resultant scaffolds were fixed and analyzed histologically. Results It was found that the decellularization process effectively removed the cellular and nuclear material while retaining native the 3D ECM of lung tissue. The architecture of the collagen and elastic fibers networks were preserved as comparable to the native lungs. Furthermore, the basement membranes of the bronchiolar and interalveolar septa were intact. Conclusions This methodology is expected to allow decellularization of human lung tissues and permits future scientific exploration in tissue engineering.

Cuttlefish bone scaffold for tissue engineering: a novel hydrothermal transformation, chemical-physical, and biological characterization

2012 ◽  
Vol 2 (10) ◽  
pp. 99-106 ◽  
Author(s):  
Elisa Battistella ◽  
Silvia Mele ◽  
Ismaela Foltran ◽  
Isidoro Giorgio Lesci ◽  
Norberto Roveri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Scaffold ◽  
Biological Characterization ◽  
Hydrothermal Transformation

Estimation of Biomechanical Stimulus in Bone Scaffolds in Vivo: Multi-Scale Finite Element Model

2010 ◽  
Author(s):  
Alireza Roshan-Ghias ◽  
Alexandre Terrier ◽  
Dominique P. Pioletti
Keyword(s):  
Tissue Engineering ◽  
Finite Element ◽  
Bone Formation ◽  
Element Model ◽  
Bone Scaffold ◽  
Synthetic Bone ◽  
Multi Scale ◽  
Bone Scaffolds ◽  
Complex Process

Bone formation inside a scaffold is a complex process involving different phenomena, one of which being the mechanical stimulation (1,2). Our goal in bone tissue engineering is, through mechanical considerations, confer osteoinductivity to a synthetic bone scaffold.

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