Antioxidant properties of Neu2000 on mitochondrial free radicals and oxidative damage

2013 ◽  
Vol 27 (2) ◽  
pp. 788-797 ◽  
Author(s):  
Nishant P. Visavadiya ◽  
Melanie L. McEwen ◽  
Jignesh D. Pandya ◽  
Patrick G. Sullivan ◽  
Byoung Joo Gwag ◽  
...  
Keyword(s):  
Free Radicals ◽  
Oxidative Damage ◽  
Antioxidant Properties

scholarly journals Antioxidant strategies for Alzheimer's disease

2002 ◽  
Vol 61 (2) ◽  
pp. 191-202 ◽  
Author(s):  
Michael Grundman ◽  
Patrick Delaney
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Vitamin E ◽  
Oxidative Damage ◽  
Functional Decline ◽  
Antioxidant Properties ◽  
Epidemiological Studies ◽  
Clinical Value ◽  
Antioxidant Defences ◽  
Ad Prevention

Oxidative damage is present within the brains of patients with Alzheimer's disease (AD), and is observed within every class of biomolecule, including nucleic acids, proteins, lipids and carbohydrates. Oxidative injury may develop secondary to excessive oxidative stress resulting from β-amyloid-induced free radicals, mitochondrial abnormalities, inadequate energy supply, inflammation or altered antioxidant defences. Treatment with antioxidants is a promising approach for slowing disease progression to the extent that oxidative damage may be responsible for the cognitive and functional decline observed in AD. Although not a uniformly consistent observation, a number of epidemiological studies have found a link between antioxidant intake and a reduced incidence of dementia, AD and cognitive decline in elderly populations. In AD clinical trials molecules with antioxidant properties such as vitamin E andGinkgo bilobaextract have shown modest benefit. A clinical trial with vitamin E is currently ongoing to determine if it can delay progression to AD in individuals with mild cognitive impairment. Combinations of antioxidants might be of even greater potential benefit for AD, especially if the agents worked in different cellular compartments or had complementary activity (e.g. vitamins E, C and ubiquinone). Naturally-occurring compounds with antioxidant capacity are available and widely marketed (e.g. vitamin C, ubiquinone, lipoic acid, β-carotene, creatine, melatonin, curcumin) and synthetic compounds are under development by industry. Nevertheless, the clinical value of these agents for AD prevention and treatment is ambiguous, and will remain so until properly designed human trials have been performed.

scholarly journals Ethanol Extract of Maclura tricuspidata Fruit Protects SH-SY5Y Neuroblastoma Cells against H2O2-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling

2021 ◽  
Vol 22 (13) ◽  
pp. 6946
Author(s):  
Weishun Tian ◽  
Suyoung Heo ◽  
Dae-Woon Kim ◽  
In-Shik Kim ◽  
Dongchoon Ahn ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Oxidative Damage ◽  
Cell Damage ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Biologically Active ◽  
Mitogen Activated Protein Kinase ◽  
Protective Effects ◽  
Neuroprotective Effects

Free radical generation and oxidative stress push forward an immense influence on the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. Maclura tricuspidata fruit (MT) contains many biologically active substances, including compounds with antioxidant properties. The current study aimed to investigate the neuroprotective effects of MT fruit on hydrogen peroxide (H2O2)-induced neurotoxicity in SH-SY5Y cells. SH-SY5Y cells were pretreated with MT, and cell damage was induced by H2O2. First, the chemical composition and free radical scavenging properties of MT were analyzed. MT attenuated oxidative stress-induced damage in cells based on the assessment of cell viability. The H2O2-induced toxicity caused by ROS production and lactate dehydrogenase (LDH) release was ameliorated by MT pretreatment. MT also promoted an increase in the expression of genes encoding the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). MT pretreatment was associated with an increase in the expression of neuronal genes downregulated by H2O2. Mechanistically, MT dramatically suppressed H2O2-induced Bcl-2 downregulation, Bax upregulation, apoptotic factor caspase-3 activation, Mitogen-activated protein kinase (MAPK) (JNK, ERK, and p38), and Nuclear factor-κB (NF-κB) activation, thereby preventing H2O2-induced neurotoxicity. These results indicate that MT has protective effects against H2O2-induced oxidative damage in SH-SY5Y cells and can be used to prevent and protect against neurodegeneration.

Comparative Evaluation of Onion Germplasm (Allium Cepa L.) Of Bangladesh for Physical And Antioxidant Properties

2021 ◽  
Vol 19 (1) ◽  
pp. 113-123
Author(s):  
PK Dash ◽  
S Das ◽  
MA Mannan ◽  
M Jahan
Keyword(s):  
Free Radicals ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
Physicochemical Analysis ◽  
The Other ◽  
Antioxidant Compounds ◽  
Visual Methods ◽  
Ic50 Value ◽  
Onion Germplasm

Determination of antioxidant activity is one of the key elements for understanding the biological properties of a widely used spice onion (A. cepa). In contrast, physical properties help understand the textural quality of onion necessary for postharvest operations of sorting and grading in particular. Considering the physicochemical importance of onion, the present study focused on the determination of physical attributes and antioxidant content of the onion germplasm in Bangladesh using visual methods and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The five-onion germplasms (BARI Piaz-2, BARI Piaz-3, BARI Piaz-4, BARI Piaz-5, Faridpuri Vati) collected from selected onion growers of the Department of Agricultural Extension (DAE) of the southwestern part of Bangladesh were used as treatments for physicochemical analysis at the Horticulture laboratory of the Agrotechnology Discipline, Khulna University, Khulna, Bangladesh during the period from February to August 2019. The laboratory study was laid out in Completely Randomized Design (CRD) with three replications. Most of the physical characteristics of onion were highest in germplasm-1 than those of the other germplasm tested. The results showed that the DPPH free radicals were scavenged by all onion germplasm extracts in a concentration-dependent pattern. The highest IC50 value (238.10 ppm) was noticed in germplasm-3 and the lowest in germplasm-5 (161.29 ppm) with no statistical difference from germplasm-4 (172.41 ppm). The lower IC50 value indicating that onion germplasms-5 and -4 extracts were more potent in scavenging free radicals than the other onion germplasms. Thus, the low IC50 value facilitated the germplasm-5 and -4 to be enriched with antioxidant compounds significantly in higher amounts than the others. SAARC J. Agric., 19(1): 113-123 (2021)

scholarly journals Protective Effect of Quercetin against H2O2-Induced Oxidative Damage in PC-12 Cells: Comprehensive Analysis of a lncRNA-Associated ceRNA Network

2020 ◽  
Vol 2020 ◽  
pp. 1-22
Author(s):  
Zheyu Zhang ◽  
Pengji Yi ◽  
Min Yi ◽  
Xiaoliang Tong ◽  
Xin Cheng ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Messenger Rna ◽  
Noncoding Rna ◽  
Expression Profiles ◽  
Damage Model ◽  
Antioxidant Properties ◽  
Pc 12 Cells ◽  
Cerna Network

Quercetin is a bioflavonoid with potential antioxidant properties. However, the mechanisms underlying its effects remain unclear. Herein, we focused on integrating long noncoding RNA (lncRNA), microRNA (miRNA), and messenger RNA (mRNA) sequencing of PC-12 cells treated with quercetin. We treated PC-12 cells with hydrogen peroxide to generate a validated oxidative damage model. We evaluated the effects of quercetin on PC-12 cells and established the lncRNA, miRNA, and mRNA profiles of these cells. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of these RNAs were conducted to identify the key pathways. Quercetin significantly protected PC-12 neuronal cells from hydrogen peroxide-induced death. We identified 297, 194, and 14 significantly dysregulated lncRNAs, miRNAs, and mRNAs, respectively, associated with the antioxidant effect of quercetin. Furthermore, the phosphatidylinositol-3-kinase/protein kinase B pathway was identified as the crucial signalling pathway. Finally, we constructed a lncRNA-associated competing endogenous RNA (ceRNA) network by utilizing oxidative damage mechanism-matched miRNA, lncRNA, and mRNA expression profiles and those changed by quercetin. In conclusion, quercetin exerted a protective effect against oxidative stress-induced damage in PC-12 cells. Our study provides novel insight into ceRNA-mediated gene regulation in the progression of oxidative damage and the action mechanisms of quercetin.

scholarly journals PENENTUAN KADAR VITAMIN C BEBERAPA JENIS CABAI (Capsicum sp.) DENGAN SPEKTROFOTOMETRI UV-VIS

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Lilis Rosmainar Tambunan ◽  
Widia Ningsih ◽  
Ni Putu Ayu ◽  
Haula Nanda
Keyword(s):  
Free Radicals ◽  
Immune System ◽  
Vitamin C ◽  
Oxidative Damage ◽  
Spectrophotometric Method ◽  
Fruits And Vegetables ◽  
Preliminary Test ◽  
The Body ◽  
Red Pepper ◽  
Pepper Sample

Vitamin C is one of the nutrients that act as antioxidants and effectively overcome free radicals that can damage cells or tissues, including protecting the lens from oxidative damage caused by radiation. Vitamin C is widely found in fruits, and vegetables, one of them in chili. Vitamin C in chili has a function as a good antioxidant for the body (able to increase the immune system absorbed by calcium in the body. This study aims to determine the levels of vitamin C contained in some types of chili using UV-Vis spectrophotometry method and conduct a preliminary test. The highest levels of vitamin C obtained by using spectrophotometric method at 200 nm wavelength were on red curly pepper (50 g/100 g) and followed by chili jablay orange-red (38 g/100 g), green cayenne (29 g/100 g), red pepper large (22 g/100 g), and large green chili (9 g/100 g). While based on preliminary test it was found that all chilies contain saponins, as well as only large red pepper (sample 1) and red curly pepper (sample 4) containing flavonoids Keywords: capsicum, chilly, spectrophotometry

scholarly journals Synthesis and DPPH scavenging assay of reserpine analogues, computational studies and in silico docking studies in AChE and BChE responsible for Alzheimer's disease

2015 ◽  
Vol 51 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Muhammad Yar ◽  
Muhammad Arshad ◽  
Ariba Farooq ◽  
Mazhar Amjad Gilani ◽  
Khurshid Ayub ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Free Radicals ◽  
In Silico ◽  
Density Functional ◽  
Neurodegenerative Disorder ◽  
Antioxidant Properties ◽  
Docking Studies ◽  
In Silico Docking ◽  
Ic50 Values

Alzheimer's disease (AD) is a fast growing neurodegenerative disorder of the central nervous system and anti-oxidants can be used to help suppress the oxidative stress caused by the free radicals that are responsible for AD. A series of selected synthetic indole derivatives were biologically evaluated to identify potent new antioxidants. Most of the evaluated compounds showed significant to modest antioxidant properties (IC50 value 399.07 140.0±50 µM). Density Functional Theory (DFT) studies were carried out on the compounds and their corresponding free radicals. Differences in the energy of the parent compounds and their corresponding free radicals provided a good justification for the trend found in their IC50 values. In silico, docking of compounds into the proteins acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), which are well known for contributing in AD disease, was also performed to predict anti-AD potential.

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