Comparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro tests

Background: Cancer is a disease characterized by the invasion and uncontrolled growth of cells. One of the best ways to minimize the harmful effects of mutagens is through the use of natural antimutagens. In this regard, the search for new antimutagens that act in the chemoprevention could represent a promising field in this area. Objective: In this study biological potential of 11 fractions from Coccoloba uvifera L. leaf hexane extract was evaluated by several in vitro tests. Methods: Leaves were lyophilized and hexane extraction was performed. The extract was fractionated by column chromatography with hexane, ethyl acetate, and methanol. The antimutagenic (Ames test), antiproliferative (MTT test), and antioxidant capacity (DPPH, ABTS, and ferrous ion chelation) of the fractions were evaluated. Results: Fractions 4, 6, 8, and 9 have antimutagenic activity (against sodium azide in strain TA100), fraction 11 showed antiproliferative capacity (IC50 of 24 ± 9 μg/mL in cells of HCT 116). The fractions with the highest activity were analyzed by HPLC-MS and lupeol, acacetin, and β-sitosterol were identified. Conclusion: This study demonstrates, for the first time, the bioactivity of C. uvifera leaf as a new source of high biological value compounds (HBVC), which can be of interest to the food and pharmaceutical industries.

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Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Plants ◽

10.3390/plants10030490 ◽

2021 ◽

Vol 10 (3) ◽

pp. 490

Author(s):

Martin Sák ◽

Ivana Dokupilová ◽

Šarlota Kaňuková ◽

Michaela Mrkvová ◽

Daniel Mihálik ◽

...

Keyword(s):

Cell Wall ◽

Vitis Vinifera ◽

Cell Cultures ◽

Trichoderma Viride ◽

Vitis Vinifera L ◽

Treated Plant ◽

Abiotic Elicitors ◽

In Vitro Cell Cultures ◽

Red Grape

The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

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Effect of in vitro gastrointestinal digestion on phenolic compounds and antioxidant properties of soluble and insoluble dietary fibers derived from hulless barley

Journal of Food Science ◽

10.1111/1750-3841.15592 ◽

2021 ◽

Vol 86 (2) ◽

pp. 628-634

Author(s):

Yulin Zhu ◽

Shihua Yang ◽

Yonghua Huang ◽

Jiaqi Huang ◽

Yongqiang Li

Keyword(s):

Phenolic Compounds ◽

Antioxidant Properties ◽

Dietary Fibers ◽

Gastrointestinal Digestion ◽

Hulless Barley ◽

In Vitro Gastrointestinal Digestion

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Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv. Syrah grape pomace extract

Food & Function ◽

10.1039/c8fo02534g ◽

2019 ◽

Vol 10 (4) ◽

pp. 1856-1869 ◽

Cited By ~ 9

Author(s):

Joana R. Costa ◽

Manuela Amorim ◽

Ana Vilas-Boas ◽

Renata V. Tonon ◽

Lourdes M. C. Cabral ◽

...

Keyword(s):

Chemical Composition ◽

Phenolic Compounds ◽

Vitis Vinifera ◽

Human Health ◽

Grape Pomace ◽

Vitis Vinifera L ◽

Gastrointestinal Digestion ◽

Gastrointestinal Health ◽

Bioactive Properties

Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including in gastrointestinal health.

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In vitro and in vivo antioxidant properties of ferulic acid: A comparative study with other natural oxidation inhibitors

Food Chemistry ◽

10.1016/j.foodchem.2008.09.073 ◽

2009 ◽

Vol 114 (2) ◽

pp. 466-471 ◽

Cited By ~ 119

Author(s):

S ITAGAKI ◽

T KUROKAWA ◽

C NAKATA ◽

Y SAITO ◽

S OIKAWA ◽

...

Keyword(s):

Comparative Study ◽

Ferulic Acid ◽

Antioxidant Properties ◽

Oxidation Inhibitors

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Phytochemical Characterization, In Vitro Antioxidant Activity, and Quantitative Analysis by Micellar Electrokinetic Chromatography of Hawthorn (Crataegus pubescens) Fruit

Journal of Food Quality ◽

10.1155/2018/2154893 ◽

2018 ◽

Vol 2018 ◽

pp. 1-11 ◽

Cited By ~ 5

Author(s):

Francisco Erik González-Jiménez ◽

Juan Alfredo Salazar-Montoya ◽

Graciano Calva-Calva ◽

Emma Gloria Ramos-Ramírez

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Micellar Electrokinetic Chromatography ◽

Antioxidant Properties ◽

Total Phenolic ◽

Electrokinetic Chromatography ◽

Polyphenol Profile ◽

Gallic Acid Equivalent ◽

Dry Fruit

Due to their antioxidant properties, polyphenolic compounds are considered beneficial for human health. In this work, we investigated the polyphenol profile and antioxidant activity of edible tejocote (Crataegus pubescens) fruit extracts by micellar electrokinetic chromatography (MEKC) and HPLC/UV. The major phenolic compounds in the pulp extracts were (+)-catechin (9.17 ± 0.20 mg/100 mg dry fruit), (−)-epicatechin (4.32 ± 0.11 mg/100 mg dry fruit), and chlorogenic acid (5.60 ± 0.24 mg/100 mg dry fruit). The total phenolic content was 168.6 ± 0.9 mg gallic acid equivalent/g dry fruit; the total proanthocyanidin content was 84.6 ± 1.4 mg cyanidin/100 g dry fruit; and the total flavonoid content was 55.89 ± 1.43 mg quercetin/g dry fruit. Interestingly, procyanidins (dimers, trimers, and tetramers of (−)-epicatechin and (+)-catechin) were detected in the extract. This is the first study reporting the presence of polymeric polyphenols in Crataegus pubescens fruit. Accordingly, these fruits demonstrate great potential as a natural source of antioxidant phenolic compounds and could therefore be used as a nutraceutical and functional food.

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Comparative Phytochemical, Antioxidant, and Hemostatic Studies of Extract and Four Fractions from Paulownia Clone in Vitro 112 Leaves in Human Plasma

Molecules ◽

10.3390/molecules25194371 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4371

Author(s):

Weronika Adach ◽

Jerzy Żuchowski ◽

Barbara Moniuszko-Szajwaj ◽

Malgorzata Szumacher-Strabel ◽

Anna Stochmal ◽

...

Keyword(s):

Oxidative Stress ◽

Phenolic Compounds ◽

Human Plasma ◽

Antioxidant Properties ◽

Plasma Lipid ◽

Inhibitory Effect ◽

Climate Conditions ◽

Positive Effects ◽

Chemical Content

Background: The Paulownia Clone in Vitro 112, known as oxytree or oxygen tree, is a hybrid clone of the species Paulownia elongata and Paulownia fortunei (Paulowniaceae). The oxytree is a fast-growing hybrid cultivar that can adapt to wide variations in edaphic and climate conditions. In this work, Paulownia Clone in Vitro 112 leaves were separated into an extract and four fractions (A–D) differing in chemical content in order to investigate their chemical content using LC-MS analysis. The extract and fractions were also evaluated for their anticoagulant and antioxidant properties in a human plasma in vitro. Results: The Paulownia leaf extract contained mainly phenolic compounds (e.g., verbascoside), small amounts of iridoids (e.g., aucubin or 7-hydroxytometoside) and triterpenoids (e.g., maslinic acid) were also detected. Our results indicate that the extract and fractions have different effects on oxidative stress in human plasma treated with H2O2/Fe in vitro, which could be attributed to differences in their chemical content. For example, the extract and all the fractions, at the two highest concentrations of 10 and 50 µg/mL, significantly inhibited the plasma lipid peroxidation induced by H2O2/Fe. Fractions C and D, at all tested concentrations (1–50 µg/mL) were also found to protect plasma proteins against H2O2/Fe-induced carbonylation. The positive effects of fraction C and D were dependent on the dose. Conclusions: The extract and all four fractions, but particularly fractions C and D, which are rich in phenolic compounds, are novel sources of antioxidants, with an inhibitory effect on oxidative stress in human plasma in vitro. Additionally, the antioxidant potential of fraction D may be associated with triterpenoids.

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