Spectrofluorimetric analysis of certain 4-quinolone in pharmaceuticals and biological fluids

SummaryThe preparation of a monoclonal antibody (MAB) against high molecular weight (HMW) urokinase light chain (20,000 Mr) is described. This MAB was immobilized and the resulting immunosorbent was used to isolate urokinase starting with an impure commercial preparation, fresh urine, spent tissue culture media, or E. coli broth without preliminary dialysis or concentration steps. Monospecific antibodies appear to provide a rapid single step method of purifying urokinase, in high yield, from a variety of biological fluids.

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The Measurement of Heparin and Other Therapeutic Sufphated Polysaccharides in Plasma, Serum and Urine

Thrombosis and Haemostasis ◽

10.1055/s-0038-1660086 ◽

1985 ◽

Vol 54 (03) ◽

pp. 630-634 ◽

Cited By ~ 19

Author(s):

J Dawes ◽

C V Prowse ◽

D D Pepper

Keyword(s):

Biological Fluids ◽

Anticoagulant Activity ◽

Dose Range ◽

Plasma Concentrations ◽

Heparin Therapy ◽

Competitive Binding Assay ◽

First Order Kinetics ◽

Activity Measurements ◽

Heparin Activity ◽

Dose Dependent

SummaryThe competitive binding assay described will specifically and accurately measure concentrations of administered heparin in biological fluids with a sensitivity of 60 ng ml-1. Neither endogenous glycosaminoglycans, nor plasma proteins such as ATIII and PF4 interfere in the assay. Semi-synthetic highly sulphated heparinoids and LMW heparin can also be measured. Using this assay heparin clearance followed simple first-order kinetics over the dose range 100-5,000 units, but the half-life was strongly dose-dependent. There was good correlation with heparin activity measurements by APTT and anti-Xa clotting assays. Plasma concentrations were measurable for at least 5 h following subcutaneous injection of 10,000 units of heparin. Excretion in the urine could be followed after all but the lowest intravenous dose. This assay, used in conjunction with measurements of heparin anticoagulant activity, will be valuable in the elucidation of mechanisms of action of heparin and the heparinoids, and in the assessment and management of problems related to heparin therapy.

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Synthesis and Characterization of Novel Nano-,Micro- and Macroporous Lignin Sorbents for Purification of Biological Fluids

Journal of Chemical Engineering Research Updates ◽

10.15377/2409-983x.2015.02.01.1 ◽

2015 ◽

Vol 2 (1) ◽

pp. 1-11

Author(s):

NN Chopabayeva ◽

◽

KN Mukanov

Keyword(s):

Biological Fluids ◽

Synthesis And Characterization

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Role of exosomes in diagnosis and therapy of prostate cancer

I P Pavlov Russian Medical Biological Herald ◽

10.23888/pavlovj2020283399-405 ◽

2020 ◽

Vol 28 (3) ◽

pp. 399-405

Author(s):

Fabrizio Fontana ◽

Olga A. Babenko

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Tumor Cells ◽

Biological Fluids ◽

Diagnosis And Treatment ◽

Diagnosis And Therapy ◽

The Future ◽

Important Direction ◽

Potential Biomarkers

Aim of this letter is to attract the attention of journal readers to the study of exosomes as an important direction in the development of Oncology, in particular, in the diagnosis and treatment of prostate cancer. Exosomes are produced by tumor cells and regulate proliferation, metastasis, and the development of chemoresistance. Their extraction from biological fluids allows further use of these vesicles as potential biomarkers of prostate cancer. In the future, exosomes can be successfully used in the delivery of drugs and other anti-tumor substances to cancer cells.

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POSSIBILITIES OF BIOLOGICAL AND MATHEMATICAL MODELING OF THE INFECTION CAUSED BY EPSTEIN–BARR VIRUS

PEDIATRIA Journal named after G N SPERANSKY ◽

10.24110/0031-403x-2020-99-6-226-231 ◽

2020 ◽

Vol 99 (6) ◽

pp. 226-231

Author(s):

A.V. Permyakova ◽

◽

A.V. Sazhin ◽

E.V. Melekhina ◽

A.V. Gorelov ◽

...

Keyword(s):

Mathematical Modeling ◽

Immune Regulation ◽

Epstein Barr Virus ◽

Virus Isolation ◽

Biological Fluids ◽

Infection Process ◽

Host Organism ◽

Barr Virus ◽

Virus Excretion ◽

Epstein Barr

The review presents the existing biological and mathematical models of the infection process caused by the Epstein–Barr virus. The existence of the Epstein–Barr virus in the host organism can be described by a model representing a cycle of six consecutive stages, each of them has its own independent variant of immune regulation. The phenomenon of virus excretion in biological fluids, in particular, in saliva, is modeled using differential equations. Usage of mathematical modeling allows us to supplement existing knowledge about the pathogenesis of the infectious process caused by the Epstein–Barr virus, as well as to determine threshold levels of virus isolation in non-sterile environments for the diagnosis of active forms of infection.

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Solid-phase structures of cerebrospinal fluid in diagnosis of early asymptomatic neurosyphilis

Nauchno-prakticheskii zhurnal «Patogenez» ◽

10.25557/gm.2018.4.9750 ◽

2018 ◽

pp. 56-61

Author(s):

С.Н. Шатохина ◽

Н.А. Кузнецова ◽

В.Н. Шабалин

Keyword(s):

Cerebrospinal Fluid ◽

Visual Analysis ◽

Solid Phase ◽

Biological Fluids ◽

Laboratory Methods ◽

Phase Structures ◽

Morphological Picture ◽

Diagnostic Technology

Цель проведённого исследования состояла в оценке эффективности визуального анализа твёрдофазных структур спинномозговой жидкости для диагностики ранних форм нейросифилиса. Методы. Использован метод краевой дегидратации биологических жидкостей, входящий в состав авторской диагностической технологии «Литос-система». Диагностика раннего асимптомного нейросифилиса заключается в выявлении деструктивных образований в форме овалов в морфологической картине твёрдой фазы спинномозговой жидкости. Результаты. Проведён сравнительный анализ результатов исследования спинномозговой жидкости у 19 больных с подтверждённым диагнозом «ранний асимптомный нейросифилис», полученных традиционными лабораторными методами и методом краевой дегидратации. Выявлено, что локализация овалов внутри сферолитов указывает на длительность заболевания нейросифилисом менее трёх лет, а вне сферолитов - от трех до пяти лет. Заключение. Метод краевой дегидратации позволяет диагностировать ранний асимптомный нейросифилис по наличию деструктивных образований в форме овалов в морфологической картине твёрдой фазы спинномозговой жидкости. The aim of this study was to evaluate effectiveness of visual analysis of solid-phase structures in cerebrospinal fluid to diagnose early forms of neurosyphilis. Methods. We used a method of marginal dehydration of biological fluids as a part of the author’s diagnostic technology, Litos-System. Early asymptomatic neurosyphilis is diagnosed based on detection of destructive, oval-shaped formations in the morphological picture of cerebrospinal fluid solid phase. Results. Data from analyses of cerebrospinal fluid performed with traditional laboratory methods and the method of marginal dehydration were compared for 19 patients with documented diagnosis of early asymptomatic neurosyphilis. A localization of ovals within spherulites indicated a less than a three-year duration of neurosyphilis while a localization outside spherulites indicated a duration of three to five years. Conclusion. The method of marginal dehydration allows detecting early asymptomatic neurosyphilis based on the presence of destructive, oval-shaped formations in the morphological picture of cerebrospinal fluid solid phase.

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