The effect of implantation of trenbolone acetate and oestradiol-17β in wether lambs at two initial live weights on concentrations of steroidal residues and blood glucose, urea and thyroid hormones

1988 ◽  
Vol 47 (1) ◽  
pp. 75-85 ◽  
Author(s):  
Lesley J. MacVinish ◽  
H. Galbraith
Keyword(s):  
Live Weight ◽  
Hormonal Treatment ◽  
Total Plasma ◽  
Trenbolone Acetate ◽  
Quality Diet ◽  
Residue Levels ◽  
Kidney Liver ◽  
Main Effects ◽  
Perinephric Fat ◽  
Plasma Triiodothyronine

AbstractThirty-two Border Leicester ♂ × Scottish Blackface ♀ wether lambs aged about 5 months were divided into two groups on the basis of live weight such that group Gl contained the 16 lightest lambs and group G2 the 16 heaviest. Lambs in group Gl were subdivided equally at random either to be sham-implanted controls (group C1) or to be implanted with 35 mg trenbolone acetate (TBA) + 5 mg oestradiol-17β (group T1) at 24 kg initial live weight. The lambs in group G2 were also subdivided into two groups (groups C2 and T2) and similarly treated approximately 1 month later at 37 kg initial live weight. The lambs were offered ad libitum a good-quality diet. They were slaughtered 60 days after implantation. Comparisons were made for the main effects of hormonal treatment and initial live weight.Concentrations in blood of 17-β-hydroxy-trenbolone (TBOH) and oestradiol-17β (OE) measured by radioimmunoassay peaked within 1 to 3 weeks after implantation and declined thereafter. Maximum concentrations and concentrations at slaughter respectively were 1·46 and 0·32 μg/l (group T1) and 0·78 and 0·28 μg/l (T2) for TBOH and 85 and 33 μg/1 (T1) and 59 and 37 ng/l (T2) for OE. Values up to week 7 were consistently greater in implanted animals in group T1 than in group T2. Hormonal implantation decreased the concentrations of total plasma triiodothyronine and thyroxine and urea and increased values for glucose up to week 5 or 6 after implantation. The animals in group G1 compared with G2 had, on average, variably lower concentrations in plasma of triiodothyronine, thyroxine, glucose and urea.The highest concentrations of solvent-extractable residues were obtained in samples of kidney and liver (up to about 500 ng/kg for TBOH and 180 ng/kg for OE) with intermediate levels for fat and lowest levels for muscle. Conjugated trenbolone ranging from 45 to 186 ng/kg was present in samples of kidney, liver and perinephric fat. Trenbolone acetate was detected only in samples of fat. Variable effects of live weight at implantation on residue levels were recorded.

Growth performance and body composition of wether lambs implanted at two different initial live weights with trenbolone acetate combined with oestradiol-17β

1988 ◽  
Vol 47 (1) ◽  
pp. 65-74 ◽  
Author(s):  
A. H. Sulieman ◽  
H. Galbraith ◽  
J. H. Topps
Keyword(s):  
Crude Protein ◽  
Live Weight ◽  
Hormonal Treatment ◽  
Metabolizable Energy ◽  
Lean Tissue ◽  
Trenbolone Acetate ◽  
Intermuscular Fat ◽  
Body Components ◽  
Gut Fill ◽  
Main Effects

AbstractThirty-two Border Leicester ♂ × Scottish Blackface ♀ wether lambs, aged about 5 months, were divided into two groups on the basis of live weight, such that group G1 contained the 16 lightest lambs and group G2 the 16 heaviest. Lambs in group G1 were subdivided equally at random either to be sham-implanted controls (Group C1) or to be implanted with 35 mg trenbolone acetate (TBA) + 5 mg oestradiol-17β (OE) (group T1) at 24 kg initial live weight. The lambs in group G2 were also subdivided into two groups (groups C2 and T2), and similarly treated approximately 1 month later at 37 kg initial live weight. The lambs were offered ad libitum a diet containing an estimated 12·5 MJ metabolizable energy (ME) per kg dry matter (DM) and 140 g/kg DM crude protein. Comparisons were made for the main effects of hormonal treatment and initial live weight. Both hormonal treatment and initial live weight gave increases for DM intake, gut fill, empty body weight, carcass weight and, in the half carcass side weight, weights of dissected lean tissue, bone and intermuscular fat and chemically determined DM, crude protein and lipid. Weights of mm. semitendinosus, longissimus dorsi, supraspinatus and gastrocnemius were also increased due to hormonal treatment and in group G2 lambs compared with those in group G1. When expressed as a proportion of carcass side weight, hormonal treatment effects were not significant for individual muscles and dissected carcass lean, bone and fat and chemically determined lipid and ash. Variable effects on other body components were recorded for both treatments in the absence of any significant interactions. The responses to hormonal treatment were essentially similar in groups G1 and G2. The question is raised as to the contribution of the greater food intake in implanted lambs to the maintenance of fatness in these animals.

Effect of naturally occurring and synthetic androgens on growth, body composition and muscle glucocorticoid receptors in wether lambs

1994 ◽  
Vol 58 (3) ◽  
pp. 357-364 ◽  
Author(s):  
H. Gallbraith ◽  
A. D. Berry
Keyword(s):  
Skeletal Muscle ◽  
Body Weight ◽  
Crude Protein ◽  
Binding Capacity ◽  
Live Weight ◽  
Backfat Thickness ◽  
Trenbolone Acetate ◽  
Naturally Occurring ◽  
Main Effects ◽  
Nandrolone Phenylpropionate

AbstractTwenty-five Border Leicester ♂ × Blackface ♀ wether lambs aged about 4 months and weighing on average 28·5 kg were allocated to be treated with the naturally occurring steroid testosterone or trenbolone acetate or nandrolone phenylpropionate which are steroids synthetically produced. Treatment groups were as follows: untreated controls (C); 50 mg testosterone (T); 50 mg trenbolone acetate (TA); 50 mg testosterone + 50 mg trenbolone acetate (TTA) or 50 mg nandrolone phenylpropionate (N). Implants were given at 100 and again at 63 days before slaughter. The lambs were offered to appetite a good quality diet containing, per kg dry matter, an estimated 11·0 MJ metabolizable energy and 185 g crude protein. Comparisons were made for the main effects of T and TA and also interactions between T and TA. Effects due to N were assessed statistically against untreated controls. Treatment with T, on average, increased live-weight gain (LWG), empty body weight (EBW) and reduced backfat thickness and the weight (g/kg EBW) of perirenal and retroperitoneal fat. Main effects due to TA were increases in killing-out ratio and depth of the gigot joint and reductions in backfat thickness. Treatment with N increased the empty body weight and (g/kg) carcass ash. Non-significant (P > 0·05) trends were suggested for increases in carcass crude protein due to T and TA treatments. T and TA but not N treatments exhibited marked androgenic activity in increasing the weight (mg/kg EBW) of the accessary vesicular gland. TA and N, but not T, reduced the weight (g/kg EBW) of the thymus gland.The maximum binding capacity of post-morte m skeletal muscle (m. gluteus) for (3H)-dexamethasone was reduced by TA but increased by T and N. These results suggest differences in the binding capacity of corticosteroid receptors which may be related to differences in the effects of T and TA on protein metabolism in skeletal muscle.

A note on the response of British Friesian steers to trenbolone acetate and hexoestrol, and to alternation in dietary energy intake

1982 ◽  
Vol 35 (2) ◽  
pp. 277-280 ◽  
Author(s):  
H. Galbraith ◽  
K. J. Geraghty
Keyword(s):  
Growth Hormone ◽  
Energy Intake ◽  
Live Weight ◽  
Metabolizable Energy ◽  
Dietary Energy ◽  
Plasma Urea ◽  
Trenbolone Acetate ◽  
Blood Constituents ◽  
Dietary Energy Intake ◽  
Main Effects

ABSTRACTFour steers from a group of eight British Friesian steers were implanted with 300 mg trenbolone acetate and 30 mg hexoestrol at the beginning of a 90-day trial period. The remainder were untreated. They were offered diets that varied in estimated content of metabolizable energy as follows (MJ/day): day 0 to 34 (period A), 100; day 35 to 60 (period B), 50; and day 61 to 90 (period C), 75 increasing to 110. Implanted steers gained significantly more live weight in periods A and C, and lost less in period B, than controls. Implanted steers had significantly elevated concentrations of plasma glucose in period A, and lower values for plasma urea and serum albumin throughout. Differences between control and implanted steers for the other blood constituents studied, including growth hormone, insulin and prolactin, were small and not significant. The main effects of changes in dietary energy intake on blood composition included significant increases in both groups of animals in the concentration of free fatty acids and growth hormone during underfeeding (period B). These concentrations decreased in period C, concomitant with significant increases in the concentrations of insulin and prolactin.

Growth performance and body composition of early weaned wether lambs treated with trenbolone acetate combined with oestradiol–17β

1986 ◽  
Vol 43 (1) ◽  
pp. 109-114 ◽  
Author(s):  
A. H. Sulieman ◽  
H. Galbraith ◽  
J. H. Topps
Keyword(s):  
Weight Gain ◽  
Crude Protein ◽  
Subcutaneous Fat ◽  
Live Weight ◽  
Hormonal Treatment ◽  
Metabolizable Energy ◽  
Trenbolone Acetate ◽  
Live Weight Gain ◽  
Total Fat ◽  
Gut Fill

ABSTRACTTwenty-eight Border Leicester ♂ × Scottish Blackface ♀ wether lambs aged 47 days and weighing 17 kg on average were allocated to be sham-implanted controls (group C) or implanted with trenbolone acetate (TBA) combined with oestradiol-17β (OE) as follows: 17·5 mg TBA + 2·5 mg OE (group TO1); 35 mg TBA + 5 mg OE (TO2); 52·5 mg TBA + 7·5 mg OE (TO3). The lambs were offered ad libitum a diet containing (per kg dry matter) an estimated 12·5 MJ metabolizable energy and 0·16 kg crude protein. They were slaughtered 60 days following implantation.On average hormonal treatment resulted in significant increases in (a) live-weight gain, food intake and gut fill, (b) proportions of perinephric and retroperitoneal fat in empty body, (c) carcass lean proportion, and (d) proportion of lean in shoulder and rib joints. Variable changes in the weights of m. semitendinosus, m. gastrocnemius and m. supraspinatus were recorded.Hormonal treatment, on average resulted in significant reductions in (a) killing-out proportion, (b) proportions of total fat and subcutaneous fat in carcass and (c) proportions of fat in the shoulder, rib and hind leg joints. Treated lambs had smaller thymus glands but larger accessory vesicular glands. Penile tissue was also increased in treated lambs. The proportional differences in live-weight gain and carcass fat content were 0·15 and 0·12 respectively.Differences in dose level had little effect on growth and carcass characteristics.

Blood and tissue lipid composition and lipase activity in wether lambs treated with trenbolone acetate combined with oestradiol-17 β at two different live weights

1988 ◽  
Vol 47 (1) ◽  
pp. 87-96 ◽  
Author(s):  
S. B. Singh ◽  
J. R. Scaife ◽  
H. Galbraith
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Live Weight ◽  
Hormonal Treatment ◽  
Metabolizable Energy ◽  
Lipid Concentration ◽  
Trenbolone Acetate ◽  
Heparin Plasma ◽  
Plasma Ffa ◽  
Experimental Treatments

AbstractThirty-two Border Leicester ♂ × Scottish Blackface ♀ wether lambs aged about 5 months, were divided on the basis of live weight such that group G1 contained the 16 lightest lambs and group G2 the 16 heaviest lambs. Lambs in group G1 were subdivided equally at random to be either sham-implanted controls (group C1) or to be implanted with 35 mg trenbolone acetate (TBA) + 5 mg oestradiol-17β (OE) (group T1) at 24 kg initial live weight. Lambs in group G2 were also subdivided into two groups (C2 and T2) and similarly treated approximately 1 month later at 37 kg initial live weight. Animals were offered ad libitum, a diet containing an estimated 12·5 MJ metabolizable energy and 140 g crude protein per kg dry matter. The experimental treatments lasted for 60 days.Samples of pre-heparin plasma were analysed for free fatty acid (FFA) and triglycerides (TG). Post-heparin plasma was analysed for lipoprotein lipase (LPL) activity. Samples of subcutaneous (SCAT), perinephric (PNAT), mesenteric (MAT) and intermuscular (IMAT) adipose tissue, liver and muscle, taken immediately post mortem, were analysed for total lipid concentration and fatty acid composition. Comparisons were made for the main effects of hormonal treatment and live weight.Plasma FFA concentrations in heavier lambs (group G2) were significantly higher than in group G1 from week 1 pre-implantation to week 3 post implantation. Compared with group G1, animals in group G2 had significantly higher plasma TG concentrations at weeks 1, 2, 5 and 8 when data was analysed using pre-implantation values as covariates. The lipid concentration was lower in group G1 than in group G2. In group G1 compared with group G2 there were greater proportions of C12:0 and C18:2 in SCAT and C16:1 in PNAT and lesser proportions of C18:0 in SCAT and PNAT.Significant effects due to hormonal treatment were recorded for plasma TG at weeks 1, 5, 6, 7 and 8 mainly due to increases in group T1 compared with group C1. Hormonal treatment increased the proportions of C12:0, C15:0, C16:1 and C18:1 in SCAT and C16:1 and C18:1 in IMAT; reduced the proportions of C16:0 and C18:0 in SCAT and C18:0 in MAT and IMAT and reduced the ratio of saturated to unsaturated fatty acids in SCAT and IMAT and to a lesser extent in MAT.

Growth performance and body composition of mature female sheep implanted with trenbolone acetate

1992 ◽  
Vol 54 (1) ◽  
pp. 53-58 ◽  
Author(s):  
A. H. Sulieman ◽  
H. Galbraith ◽  
J. H. Topps
Keyword(s):  
Body Composition ◽  
Subcutaneous Fat ◽  
Live Weight ◽  
Hormonal Treatment ◽  
Mature Female ◽  
Metabolizable Energy ◽  
Body Parts ◽  
Trenbolone Acetate ◽  
Intermuscular Fat ◽  
Female Sheep

AbstractThe effect of the anabolic steroid trenbolone acetate (TEA) on growth and body composition was studied in Scottish Blackface mature female sheep weighing 45 kg. The sheep were blocked by weight and randomly allocated to be untreated controls (C) or subcutaneously implanted with 20 (Tl), 40 (T2) or 60 (T3) mg TBA 60 days before slaughter. The sheep were offered to appetite a loose-mix diet which contained an estimated 11 M] metabolizable energy and 120 g crude protein per kg dry matter. Live-weight gain and food intake, on average, were not significantly affected by the hormonal treatment which significantly reduced gut fill and significantly increased empty body and chilled carcass weights. Of the carcass tissues which were dissected only weights of total lean and intermuscular fat were significantly increased following TBA implantation. The relative development of fat, lean and bone in the carcass in the hormonally treated compared with control sheep was not significantly altered. The only effects on weights of organs and body parts associated with TBA implantation were increases in fat deposition in omental and perinephric and retroperitoneal depots. There was some evidence of an increasing response to TBA treatment with increasing dose level for weights of chilled carcass, carcass lean and the ratio of subcutaneous fat to intermuscular fat.

The protein and energy nutrition of the pig. V. The effect of varying the protein and energy levels in the ‘finishing diets’ of heavy pigs

1965 ◽  
Vol 65 (3) ◽  
pp. 405-409 ◽  
Author(s):  
D. W. Robinson
Keyword(s):  
Crude Protein ◽  
Energy Levels ◽  
Live Weight ◽  
High Energy ◽  
Feeding Trial ◽  
Large White ◽  
High Energy Level ◽  
Finishing Diets ◽  
Main Effects ◽  
Carcass Measurement

1. A feeding trial was carried out using fortyeight Large White pigs, individually fed in a Danish type piggery. Six different diets prepared at two levels of digestible energy and three levels of crude protein were fed to eight replicates consisting of four hogs and four gilts per replicate. A record was maintained of the weekly live-weight gain and food was given at a defined restricted level in relation to the live weight. Carcass quality was assessed by complete dissection into visible lean, fat and bone etc.2. Of the main effects, energy, supply was without significant effect upon growth, food conversion efficiency (FCE) or any carcass characteristics except body length which was increased with a high energy level. The protein level in the diet had a significant effect upon the percentage of carcass lean and the killing-out percentage, the higher levels of protein increasing both these measurements significantly. Gilts were significantly superior to hogs in every carcass measurement although hogs grew significantly faster.

scholarly journals PHYSIOLOGICAL STATE AND PRODUCTIVITY OF ANIMALS UNDER THERMAL STRESS

2019 ◽  
Vol 51 (3) ◽  
pp. 26-31
Author(s):  
E.A. Azhmuldinov ◽  
◽  
Yu.N. Chernyshenko ◽  
M.G. Titov ◽  
◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Physiological State ◽  
Live Weight ◽  
Blood Parameters ◽  
Maximum Temperature ◽  
Total Plasma ◽  
Ambient Temperatures ◽  
Study Results ◽  
Total Plasma Protein ◽  
Negative Effect

A study was conducted of the effect of heat stress on the clinical and physiological state of male rabbits at the age of 5 months with an average live weight of 1551 g. The maximum temperature the animals were exposed to during the experiment was +42 °C. The study results showed a decrease in feed intake and an increase in water consumption, which contributed to a decrease in live weight. Hematological parameters, including hemoglobin, erythrocytes, leukocytes, total plasma protein, globulins and cholesterol increased during the action of this stress factor. It was found that the effect of high ambient temperatures on rabbits negatively affected their physiological functions; the negative effect was reflected by the morphological and biochemical blood parameters.

Effects of sources of carbohydrate and protein given at low levels with grass silage ad libitum on the performance of lactating dairy cows

1995 ◽  
Vol 1995 ◽  
pp. 163-163
Author(s):  
K. Aston ◽  
R.J. Dewhurst ◽  
W.J. Fisher ◽  
D.W.R. Davies ◽  
A.B. McAllan
Keyword(s):  
Milk Production ◽  
Live Weight ◽  
Rapeseed Meal ◽  
Grass Silage ◽  
Lactating Cows ◽  
Lactating Dairy Cows ◽  
Production Experiment ◽  
Low Levels ◽  
Main Effects ◽  
Degradable Protein

An effective strategy for milk production is to supplement silage with small amounts of high-protein concentrate based on mixed carbohydrate sources and fish and soyabean meals (Aston et al 1992). Recent work at Trawsgoed by Sargeant and McAllan (1993) using growing steers given high-digestibility grass silage supplemented with rapeseed meal (a source of highly rumen degradable protein) produced similar live-weight gains to those obtained with fish meal. Jacobs and McAllan (1992) concluded that microbial protein yield is greater when the more degradable protein source is given, indicating a better balance in the supply of nitrogen and energy nutrients within the rumen. Lactating cows given grass silage diets have a high requirement for Metabolisable Protein (MP). The source of rumen fermentable carbohydrate may be important when MP supply is derived from degradable dietary protein and hence microbial growth. This study therefore examined the effects of varying the sources of carbohydrate and protein in low levels of concentrate given with high-digestibility grass silage. The main effects observed in the milk production experiment are presented here.

Effects of underfeeding during the second gestation of Holstein-Friesian heifers. 2. Residual effects on second lactation performance

1997 ◽  
Vol 1997 ◽  
pp. 93-93
Author(s):  
J.M. Moorby ◽  
R.J. Dewhurst ◽  
W.J. Fisher ◽  
D.W.R. Davies
Keyword(s):  
Live Weight ◽  
Residual Effects ◽  
Lactation Performance ◽  
Dry Period ◽  
Treatment Groups ◽  
Holstein Friesian ◽  
Lactational Performance ◽  
Protein Feeding ◽  
Quality Diet ◽  
Ad Libitum

Previous work has shown that dry period protein feeding can have important residual effects on the subsequent lactational performance in dairy cows (Moorby et al., 1996), although the results have been variable. This variability may be due to an animals nutrient requirements during late lactation and its ability to compensate during the dry period for previously inadequate supplies. This experiment was designed to investigate the effects of heifer age and level of concentrate offered during the second half of the first lactation on the second lactation performance. The size of the Longissimus dorsi was monitored as a marker of skeletal muscle use for lactation.Thirty-eight Holstein-Friesian heifers were treated as described by Dewhurst et al. (1997). Briefly, animals were divided into 4 treatment groups differing in age at first calving (2 or 3 years old) and level of concentrate feeding for the last 18-20 weeks of their first lactation (2 kg/d, ‘L’, or 7 kg/d, ‘H’; n=11, 11, 9 and 7 for treatments 2L, 2H, 3L and 3H respectively). Six weeks before predicted calving date, animals were dried off and offered a relatively low quality diet of ad libitum access to a grass silage:straw mix (40:60 on a DM basis), designed to offer them little chance to compensate for previous differences in diet. After calving, animals were offered a diet of ad libitum access to grass silage plus 8 kg/d concentrate to day 120 of lactation, and 5 kg/d thereafter. Live weight was recorded weekly. After calving, milk yields were recorded daily, and milk samples taken weekly, to week 20 of lactation.

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