The effect of application of genistein to 
Bradyrhizobium japonicum culture and its rooting 
medium on soyabean growth nodulation and nitrogen 
assimilation in the presence of nitrate

In the soyabean [Glycine max (L.) Merr.]–B. japonicum symbiosis, genistein has been identified as one of the major compounds in soyabean seed and root extracts responsible for inducing the expression of the B. japonicum nod genes. High combined nitrogen in the growth medium inhibits nodulation and nitrogen assimilation. Two experiments were conducted to test the possibility of overcoming this inhibition by adding genistein to the rooting medium and by incubation of B. japonicum cells with genistein. One soyabean cultivar was used in the first experiment, and two in the second experiment. The experiments were conducted in a glasshouse using a completely randomized design with three rooting medium nitrate concentrations (0, 5 and 10 mM) and four genistein treatments. The genistein treatments were 0 (control), incubation of B. japonicum cells with 5 μM genistein, and regular watering with 5 μM or 20 μM genistein. A two way interaction existed in the first experiment, and two and three way interactions existed in the second experiment. Root growth was inhibited by repeated watering with 20 μM genistein. Weight per nodule was greater at 5 mM than at 0 mM nitrate. At 10 mM nitrate watering with genistein resulted in significant increases in nodule dry weight per plant. Shoot nitrogen contents were significantly increased at 5 mM nitrate by genistein incubation and watering with 20 μM genistein. Watering with 5 μM genistein significantly increased nodule nitrogen concentrations at both 5 and 10 mM nitrate. The two soyabean cultivars responded differently to the genistein and nitrate treatments in terms of nodule number, nodule weight, leaf nitrogen concentration and nodule nitrogen content. Genistein could, at least partially, overcome the inhibition of soyabean nodulation and nitrogen assimilation by nitrate.

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Cytogenotoxic effects of nitrogen on hydroponic lettuce

Bioscience Journal ◽

10.14393/bj-v36n0a2020-53643 ◽

2020 ◽

Vol 36 ◽

Author(s):

Alcione da Silva Arruda ◽

Wesley Costa Silva ◽

Roberta Camargos de Oliveira ◽

Ernane Miranda Lemes ◽

Gabriela da Silva Guimarães ◽

...

Keyword(s):

Nitrogen Concentration ◽

Calcium Nitrate ◽

Dry Weight ◽

Positive Control ◽

Leaf Nitrogen ◽

Nitrogen Accumulation ◽

Fresh Weight ◽

Shoot Dry Weight ◽

Nutrient Film Technique ◽

Nitrogen Concentrations

Nitrogen accumulation in hydroponically-grown lettuce may pose a health risk to consumers. Thus, the objective of this study was to analyze different concentrations of nitrogen applications in hydroponic lettuce cultivation and their effect on toxicity, cytotoxicity and genotoxicity. A nutrient film technique (NFT) hydroponic system was used to grow the lettuce variety “Vanda.” The treatments consisted of different concentrations of nitrogen (in the form of calcium nitrate) in Furlani solution (75, 100, 125 and 150%), a negative and a positive control. The following commercial characteristics were measured: plant fresh weight (PFW), root fresh weight (RFW), shoot fresh weight (SFW), shoot diameter (SD), root dry weight (RDW), shoot dry weight (SDW) and leaf nitrogen (LN). Cytogenotoxicity was indicated by toxicity, cytotoxicity and genotoxicity, which were in turn determined by root length, the mitotic index, chromosomal aberrations and the presence of micronuclei. The nitrogen concentrations used in this experiment did not cause phenotypic toxicity or cytotoxicity in lettuce roots. The most severe genotoxicity was observed at the 125% nitrogen concentration, which nevertheless did not affect commercial characteristics. Although nitrogen fertilization provides great benefits to agriculture, such as greater yields, indiscriminate use should be avoided since concentrations above recommended rates may induce genotoxicity.

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Bioherbicidal Actions of Common Purslane on Seed Germination and Growth of Some Crop and Weed Species

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/910/1/012107 ◽

2021 ◽

Vol 910 (1) ◽

pp. 012107

Author(s):

Saber W. Hamad

Keyword(s):

Seed Germination ◽

Negative Impact ◽

Growth Parameters ◽

Dry Weight ◽

Weed Species ◽

Root Extracts ◽

Randomized Design ◽

Completely Randomized Design ◽

Common Purslane ◽

Germination And Growth

Abstract This study was conducted to examine allelopathic actions of Common Purslane Portulaca oleracea L. aqueous shoot and root extracts on germination of seeds and some growth parameters of wheat (Triticum aestivum) and rapeseed (Brassica napus). The experiment was performed in sterilized Petri dishes for one week at 22 °C. The experiment was arranged for completely randomized design. The concentrations were chosen for this study as (0%, 3%, 6% and 9%). The results indicated that the higher concentrations (6% and 9%) of both shoot and root extracts of common purslane caused significant reduction in seed germination whereas the lowest concentration caused the least negative impact on seed germination. Other growth polarimeters such as shoot and root length and shoot and root oven dry weight were significantly inhibited by the application of both Common Purslane aqueous shoot and root extracts at concentrations (6% and 9%) in wheat T. aestivum and rapeseed B. napus. However, dicot B. napus turned out to be more effected than monocot T. aestivum. The findings of this study suggest that common purslane aqueous shoot and root extracts can be recommended to utilize as bio-herbicide to supress seed germination and growth of weeds.

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Effect of planting date and density on calendula and peppermint herbs

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v1i1.4241 ◽

2014 ◽

Vol 1 (1) ◽

pp. 25-29

Author(s):

Rahim Mohammadian ◽

Behnam Tahmasebpour ◽

Peyvand Samimifar

Keyword(s):

Mutual Effect ◽

Dry Weight ◽

Planting Date ◽

Mass Reduction ◽

Flower Number ◽

Planting Dates ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Total Maximum

A factorial experiment was conducted with a completely randomized design to evaluate the effects of planting date and density on calendula herbs and peppermint. It had 3 replicates and was done in Khosroshahr research farm, Tabriz in 2006. Under studied factors were: 3 planting dates (10 May, 25 May and 10 June) in 4 densities (25, 35, 45, 55) of the plant in square meters. The results of variance a nalysis showed that there was 1% probability significant difference between the effects of planting date and bush density on the leave number, bush height and the bush dry weight. But the mutual effect of the plant date in mentioned traits density was insignificant. Regarding the traits mean comparison, the total maximum dry weight was about the 55 bush density in mm. Also, the bush high density in mm causes the bush growth and its mass reduction. When there is the density grain, the flower number will increase due to bush grain in surface unit. Overall, we can conclude that 10 June planting and 45 bush density in mm is the most suitable items and results in favored production with high essence for these crops.

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Respon Akar Bibit Aren Genjah (Arenga pinnata) Di Pembibitan Pada Pemberian Dosis dan Interval Pupuk Organik Cair Nasa

Agrifarm : Jurnal Ilmu Pertanian ◽

10.24903/ajip.v7i1.368 ◽

2018 ◽

Vol 7 (1) ◽

pp. 28

Author(s):

Yetti Elidar

Keyword(s):

Organic Fertilizer ◽

Dry Weight ◽

Treatment Combination ◽

Root Dry Weight ◽

Randomized Design ◽

Dose Study ◽

Palm Sugar ◽

Wet Weight ◽

Completely Randomized Design ◽

Leaf Dry Weight

Research on the response of roots of palm sugar palm seeds (Arenga pinnata) in nurseries at doses and intervals of Nasa liquid organic fertilizer. Aims to determine the dosage, interval and combination of dosages and fertilization intervals with Nasa liquid organic fertilizer which can provide the best dry weight of the roots in the nursery. The research design used was a Completely Randomized Design (CRD) with 3x3 factorial experiments and each treatment was repeated 8 (eight) times, consisting of: the first factor was the treatment of POC Nasa dose in a concentration of 3 cc POC Nasa per liter of water (D) consists of 3 levels, namely: d1 = 300 ml POC Nasa, d2 = 400 ml POC Nasa, d3 = 500 ml POC Nasa, while the second factor is the treatment of POC Nasa Interval (I) consisting of 3 levels, namely: i1 = 2 once a week, i2 = once every 3 weeks, i3 = once every 4 weeks. The results of the POC Nasa dose study had a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level are: d2 (400 ml of Nasa liquid organic fertilizer), the interval of liquid organic fertilizer Nasa has a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level is: i1 (once every 2 weeks). The treatment combination has no significant effect on all parameters.

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Perlakuan Berbagai Dosis Pupuk ZA Pertumbuhan Dan Hasil Tanaman Bayam Merah (Amaranthus gangeticus)

Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis ◽

10.30737/agrinika.v5i2.1946 ◽

2021 ◽

Vol 5 (2) ◽

pp. 180

Author(s):

Edy Kustiani ◽

Mariyono Mariyono ◽

Bela Citra Ayuningtyas

Keyword(s):

Analysis Of Variance ◽

Nutritional Value ◽

Environmental Design ◽

Dry Weight ◽

Growth And Yield ◽

Dose Treatment ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

The Ideal

Red spinach (Amaranthus gangeticus) is one of the most popular vegetables because of its high nutritional value and easy cultivation. Efforts to increase the production of red spinach are to fertilize to meet the needs of both macro and micronutrients. This study aimed to determine the effect of ZA fertilizer dose on the growth and yield of red spinach. The environmental design used was a non-factorial, completely randomized design with four replications. The factors tested were the dose of ZA fertilizer which consisted of 6 levels, namely: without ZA fertilizer (D0); 0.4 gr/polybag (D1); 0.8 gr/polybag (D2); 1.2 gr/polybag (D3); 1.6 gr/polybag (D4); 2 gr/polybag (D5). The data obtained were analyzed using analysis of variance, and if the results were significantly different, the BNT test was continued at a 5% level. The growth and yield of red spinach showed significant differences due to the ZA fertilizer dose treatment. The ZA fertilizer dose treatment of 1.2 g/polybag (D3) resulted in the highest plant height, the number of leaves, and leaf area of red spinach at 14, 21, and 28 days after planting. The ZA fertilizer dose treatment of 1.2 g/polybag (D3) resulted in the highest wet and dry weight plants of 49.15 and 4.38 grams, respectively. The ZA 1.2 gr/polybag dose is the ideal dose to increase the productivity of red spinach.Bayam merah (Amaranthus gangeticus) termasuk sayuran penting yang disukai masyarakat karena bernilai gizi tinggi dan mudah pembudidayaannya. Upaya peningkatan hasil bayam merah salah satunya dengan pemupukan untuk menambah nutrisi yang dibutuhkan tanaman. Tujuan dari penelitian ini adalah mempelajari respon pertumbuhan dan hasil tanaman bayam merah pada perlakuan dosis pupuk ZA. Pelaksanaan penelitian ini menggunakan Rancangan Acak Lengkap (RAL) satu faktor. Faktor yang diujikan adalah dosis pupuk ZA, terdiri 6 taraf, yaitu: tanpa pupuk ZA (D0); 0.4 gr/polybag (D1); 0.8 gr/polybag (D2); 1.2 gr/polybag (D3); 1.6 gr/polybag (D4); 2 gr /polybag (D5). Percobaan dilakukan dengan ulangan sebanyak 4 kali. Analisis data menggunakan analysis of variance (ANOVA), apabila berbeda nyata dilanjutkan uji Beda Nyata Terkecil (BNT) taraf 5%. Perlakuan dosis pupuk ZA berpengaruh nyata terhadap pertumbuhan dan hasil tanaman bayam merah. Perlakuan dosis pupuk ZA 1.2 gr/polybag (D3) menghasilkan tinggi, jumlah dan luas daun tanaman bayam merah tertinggi pada pengamatan umur 14, 21, dan 28 HST. Dosis pupuk ZA 1.2 gr/polybag (D3) juga menunjukkan berat basah dan berat kering tanaman tertinggi sebesar 49.15 dan 4.38 gram. Dosis ZA 1.2 gr/polybag merupakan dosis ideal untuk meningkatkan produktivitas tanaman bayam merah.

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Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Marine Drugs ◽

10.3390/md17080460 ◽

2019 ◽

Vol 17 (8) ◽

pp. 460 ◽

Cited By ~ 9

Author(s):

Juan Eduardo Sosa-Hernández ◽

Laura Isabel Rodas-Zuluaga ◽

Carlos Castillo-Zacarías ◽

Magdalena Rostro-Alanís ◽

Reynaldo de la Cruz ◽

...

Keyword(s):

Light Intensity ◽

Biomass Yield ◽

Nitrogen Concentration ◽

Biomass Productivity ◽

Dry Weight ◽

High Light Intensity ◽

High Biomass ◽

Porphyridium Purpureum ◽

Nitrogen Concentrations ◽

Low Nitrogen

Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity.

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Germination test of Eucalyptus phaeotricha seeds

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v22n9p653-657 ◽

2018 ◽

Vol 22 (9) ◽

pp. 653-657 ◽

Cited By ~ 1

Author(s):

Carlos E. Affonso ◽

Givanildo Z. da Silva ◽

Tatiane S. Jeromini ◽

Cibele C. Martins

Keyword(s):

Dry Weight ◽

Water Volume ◽

Conduction Time ◽

Germination Test ◽

Randomized Design ◽

Temperature Regimes ◽

Seed Analysis ◽

Completely Randomized Design ◽

Mean Time ◽

Temperature Conduction

ABSTRACT The rules for seed analysis do not have recommendations for conducting the germination test of Eucalyptus phaeotricha seeds. Thus, the aim of this study was to determine the best substrate, temperature, conduction time, and substrate moistening level for the germination test of E. phaeotricha seeds. First count of germination, germination, germination speed index and mean time of germination were evaluated. The first experiment was carried out in a completely randomized design, in a factorial scheme (6 x 2), corresponding to six temperature regimes (15, 20, 25, 30, 35 °C and 20-30 °C) and two substrates (paper and sand) with four replicates, and the means were compared by Tukey test at 0.05 probability level. In the second experiment, the germination test was installed with the most favorable substrate and temperature identified in the previous step, evaluating substrate moistening levels equivalent to 1.0, 1.5, 2.0, 2.5 and 3.0 times the dry weight of the substrate; due to the quantitative nature of the treatments, the data were subjected to regression analysis and curve fitting. The germination test of E. phaeotricha seeds should be conducted at 30 °C on paper, moistened with a water volume of 2.2 times the dry weight of the paper, with initial and final counts at 6 and 14 days after sowing, respectively.

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PENGARUH INTENSITAS PEMOTONGAN TERHADAP PRODUKTIVITAS Indigofera zollingeriana

ZOOTEC ◽

10.35792/zot.40.2.2020.29881 ◽

2020 ◽

Vol 40 (2) ◽

pp. 665

Author(s):

Ivan H.G.M. Wagiu ◽

Ch.L. Kaunang ◽

M.M. Telleng ◽

W.B Kaunang

Keyword(s):

Dry Weight ◽

Randomized Design ◽

Optimal Productivity ◽

Indigofera Zollingeriana ◽

Completely Randomized Design ◽

The Difference ◽

Cutting Intensity

THE EFFECT OF DEFOLIATION INTENSITY ON PRODUCTIVITY OF Indigofera zollingeriana. This research aims to know the effect of defoliaion intensity on productivity (fresh production, dry weight production, proportion of leaves and stems) and to know the defoliation intensity with optimal productivity of forage Indigofera zollingeriana. This research was conducted in December 2019 until March 2020. It consisted of defoliation intensity at 75cm (T75), 100cm (T100), 125cm (T125), and 150cm (T150). The design used in this experiment is Completely Randomized Design. The variables in this research were 1. fresh production leaves and stems; 2. dry weight production leaves and stems; 3. the proportion of leaves and stems dry weight production of forages Indigofera zollingeriana. The results showed that the difference of intensity defoliation had a significantly effect (P<0,01) on productivity (fresh production, dry weight production, proportion of leaves and stems) forage Indigofera zollingeriana. Defoliation intensity 75 cm have significantly (P<0,01) fresh and dry weight leaf of Indigofera zollingeriana than hight defoliation 125 cm and 150 cm. Intensity defoliation 125 cm have significantly (P<0,01) leaf-stem ratio of Indigofera zollingeriana than any other. It conclude that intensity defoliation 125 cm have highly productivity. Keywords: Indigofera zollingeriana, cutting intensity, productivity

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Pengaruh Campuran Pasir dan Serat Aren pada Kailan (Brassica oleraceae) dengan Hidroponik Substrat

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v16i1.18908 ◽

2014 ◽

Vol 16 (1) ◽

pp. 19

Author(s):

Samanhudi Samanhudi ◽

Dwi Hardjoko ◽

Riandy Adhitya

Keyword(s):

Wood Fiber ◽

Dry Weight ◽

Fresh Weight ◽

Randomized Design ◽

The Media ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Brassica Oleraceae ◽

Media Mix ◽

Leaf Greenness

Wood fiber waste of arenga produced from starch palm industry. It is not handled properly so that could be potentially cause problems for the surrounding community. Wood fiber waste of arenga is potentially used as a medium for hydroponics. The waste can be utilized to get a new composition of hydroponic media. This study aims to determine the effect of a mixture of sand with arenga fiber waste. This research was carried out by using a mixture of sand and arenga wood fiber waste as a medium. This is an experimental study using a completely randomized design. This study aims to determine the effect of a mixture of sand and arenga fiber waste for kailan growth. The results showed that the mixture of sand and arenga wood fiber waste has no effect on kailan growth. The combination of mixtures of sand and arenga fiber waste showed no interaction. Observations on plants such as plant height, number of leaves, leaf greenness levels, the fresh weight of crop stover and stover dry weight of the plant, showed a row of the highest results in the media mix washed sand, washed sand and rice husk

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Toleransi Plasma Nutfah Padi Lokal terhadap Salinitas

Buletin Plasma Nutfah ◽

10.21082/blpn.v23n1.2017.p51-58 ◽

2018 ◽

Vol 23 (1) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

Tintin Suhartini ◽

Try Zulchi

Keyword(s):

Plant Height ◽

Salinity Tolerance ◽

Negative Correlation ◽

Dry Weight ◽

Gene Bank ◽

Dead Leaf ◽

Randomized Design ◽

Highly Sensitive ◽

Number Of Leaves ◽

Completely Randomized Design

AbstractThe research was aimed to screen the local rice germplasms for salinity tolerance. The 104 local rice germplasms were derived of collection of Gene Bank of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) were tested to salinity. The activities was carried out in greenhouse of ICABIOGRAD. The 15 day old seedlings were planted in pots soil contained NaCl 0.4%. One accession of rice was planted in 2 pots, each pot contained 4 seedlings. Observation of plant symptom was conducted after 1 month planted. The experiment was carried out in a Completely Randomized Design with 2 replications. The results of the analysis showed there were different responsses among rice accesions to the treatment. Salinity caused dry and dead leaves, there were very significant differences between the varieties for plant height, dry weight plant, number of total leaves, and number of green leaves. There were a high and negative correlation of plant height and total number of leaves with percentage of dead leaves. The result showed 21 accesions were tolerant to moderately&#8722;tolerant, while Pokkali as a tolerant check, most tolerant. There were two accessions were tolerant with dead leaf percentage <50%, i.e. Tjempo Brondol (reg. 5800) and Gembira Putih (reg. 20602), whereas Pokkali as resistant check had low dead leaf percentage (16.9%) and 19 accessions were moderately-tolerant with dead leaf percentage <70%, and 82 accesions were sensitive to highly-sensitive.AbstrakPenelitian ini bertujuan untuk menyaring plasma nutfah padi yang memiliki sifat toleran terhadap salinitas. Sejumlah 104 plasma nutfah padi lokal yang berasal dari koleksi Bank Gen BB Biogen diuji terhadap salinitas. Kegiatan dilakukan di rumah kaca BB Biogen. Bibit padi berumur &plusmn;15 hari ditanam pada pot berisi tanah yang sudah dilumpurkan dengan larutan garam NaCl 0,4% (4.000 ppm). Satu aksesi padi ditanam pada 2 pot, masing masing pot berisi 4 tanaman. Setelah berumur 4 minggu dari tanam dilakukan pengamatan meliputi tinggi tanaman, bobot kering akar, bobot kering tanaman, jumlah daun total, jumlah daun hijau, dan jumlah daun mati. Pengujian menggunakan Rancangan Acak Lengkap dengan 2 ulangan. Hasil analisis varians menunjukkan terdapat perbedaan respons plasma nutfah padi terhadap perlakuan salin yang diberikan. Perbedaan sangat nyata terdapat pada tinggi tanaman, bobot kering tanaman, jumlah daun total, dan jumlah daun hijau. Terdapat korelasi yang tinggi dan negatif pada tinggi tanaman dan jumlah daun total dengan persentase daun mati. Hasil pengujian telah terpilih 21 aksesi plasma nutfah padi toleran hingga agak toleran, sedangkan Pokkali sebagai cek sangat toleran. Dua aksesi yang toleran dengan persentase daun mati <50%, yaitu Tjempo Brondol (reg. 5800) dan Gembira Putih (reg. 20602), Pokkali sebagai varietas kontrol dengan tingkat persentase daun mati 16,9%, sedangkan 19 aksesi termasuk kelompok agak toleran dengan persentase daun mati <70%, dan 82 aksesi termasuk kelompok peka hingga sangat peka.

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