A study of methods for estimating the cell content of bulk milk

1970 ◽  
Vol 37 (3) ◽  
pp. 467-480 ◽  
Author(s):  
J. K. L. Pearson ◽  
C. L. Wright ◽  
D. O. Greer
Keyword(s):  
Correlation Coefficient ◽  
Chemical Method ◽  
Correlation Coefficients ◽  
Cell Counting ◽  
Screening Tests ◽  
Cell Content ◽  
Centrifugal Method ◽  
California Mastitis Test ◽  
Bulk Milk ◽  
Close Relationship

SummaryFour methods of estimating the cell content of bulk milk were studied, correlated statistically and their degree of reproducibility estimated. The relative merits of the 4 methods are discussed, and the need for defining the object of testing before selecting a test is stressed. If designed for use in a programme for mastitis control, or the control of milk quality based on cell content, each country must choose a screening test or a precise cell-counting technique, depending on the availability of laboratory resources, extension advisory services and other economic factors.Screening tests studied were the California Mastitis Test (CMT) and the Brabant Mastitis Test (BMR), the former showing a correlation coefficient of 0·817 against the Electronic Cell Count (ECC) method in 320 samples. The more objective BMR correlated well with the ECC method, showing a correlation coefficient of 0·987 for 406 samples. Direct microscopic counts (DMC) were correlated with a centrifugal method and with a chemical method of preparing samples for electronic cell counting. For 430 samples in each case the correlation coefficients obtained were 0·930 and 0·966 respectively. The 2 ECC techniques were compared using 430 samples, and a very close relationship was found (r=0·988).For regular examination of large numbers of milk samples using the electronic cell counter we consider the chemical method of sample preparation more suitable than the centrifugal method. Differences in the reproducibility and accuracy of both tests are negligible, but the former procedure is quicker, more economical in terms of cost, and more adaptable to a national testing programme.

A COMPARISON OF SCREENING TESTS TO DETECT ABNORMAL MILK1

1969 ◽  
Vol 32 (9) ◽  
pp. 365-368 ◽  
Author(s):  
G. Nageswararao ◽  
H. E. Calbert
Keyword(s):  
Optical Density ◽  
Catalase Activity ◽  
Screening Tests ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Fresh Milk ◽  
Bulk Milk ◽  
Microscopic Count ◽  
Insignificant Correlation

The results of the Feulgen-DNA-color optical density and score, the California mastitis test (CMT) score, viscosity developed in milk samples by addition of equal volume of CMT reagent, the catalase test, the (direct microscopic count bf leucocytes, and the proportion of live leucocytes were compared using fresh, quarter, and bucket milk samples and 1 to 2 day old bulk milk samples. The Feulgen-DNA-color gave. good correlations with all the tests in all types of samples, except with the CMT of bulk samples. The CMT score and viscosity gave good correlations with all the tests on fresh milk samples and insignificant correlation with stored milk samples. The catalase test gave good correlations with all other tests in fresh samples and low but significant correlations with stored samples. Quarter and bucket milk samples contained 71% and 74% live leucocytes and bulk milk samples contained 42% live leucocytes. Holding of milk at 5 C for 5 days did not significantly change the Feulgen-DNA-color and catalase activity, slightly increased total leucocyte counts, but gradually decreased the CMT score, the viscosity, and the proportion of live leucocytes. The Feulgcn-DNA-color of milk samples containing few millions of leucocytes and the direct microscopic count of total, leucocytes gave poor reproducibility.

scholarly journals The relationship between pressure and temperature at the same level in the free atmosphere

1920 ◽  
Vol 98 (691) ◽  
pp. 235-248 ◽  
Keyword(s):  
Correlation Coefficient ◽  
Strong Evidence ◽  
Correlation Coefficients ◽  
Considerable Importance ◽  
Free Atmosphere ◽  
Close Relationship ◽  
The Relationship ◽  
True Values

An important set of correlation coefficients showing the relationship between pressure and temperature at the same level in the free atmosphere has recently been published by W. H. Dines. The coefficients are repro­duced here in Table I. These correlation coefficients, ranging as they do from 0·72 to 0·91 for the layers between 3 k and 8 k , present indisputably strong evidence of a very close relationship between pressure and temperature at the same level. Now it is easily proved that a correlation coefficient is always lowered in the numerical sense by errors of observation. Hence a point of considerable importance arises with regard to the correlation coefficients of Table I Granted that errors of observation exist in the observations from which the coefficients are calculated, what are the true values of these coefficients, the effects of errors of observation being eliminated ? It is the aim of the present paper to answer this question from the data available for the purpose.

IMPROVEMENT OF SPRING DURUM WHEAT SELECTION METHODOLOGIES UNDER CLIMATE CHANGE CONDITIONS

1970 ◽  
pp. 33-36
Author(s):  
А. I. Grabovets ◽  
V. P. Kadushkina ◽  
S. А. Kovalenko
Keyword(s):  
Grain Yield ◽  
Durum Wheat ◽  
Correlation Coefficient ◽  
Harvest Index ◽  
Unit Area ◽  
Correlation Coefficients ◽  
Chemical Mutagenesis ◽  
Main Method ◽  
The Relationship ◽  
Selection Of

With the growing aridity of the climate on the Don, it became necessary to improve the methodology for conducting the  breeding of spring durum wheat. The main method of obtaining the source material remains intraspecific step hybridization. Crossings were performed between genetically distant forms, differing in origin and required traits and properties. The use of chemical mutagenesis was a productive way to change the heredity of genotypes in terms of drought tolerance. When breeding for productivity, both in dry years of research and in favorable years, the most objective markers were identified — the size of the aerial mass, the mass of grain per plant, spike, and harvest index. The magnitude of the correlation coefficients between the yield per unit area and the elements of its structure is established. It was most closely associated with them in dry years, while in wet years it decreased. Power the correlation of the characteristics of the pair - the grain yield per square meter - the aboveground biomass averaged r = 0.73, and in dry years it was higher (0.91) than in favorable ones (0.61 - 0.70) , between the harvest and the harvest index - r = 0.81 (on average). In dry years, the correlation coefficient increased to 0.92. Research data confirms the greatest importance of the mass of grain from one ear and the plant in the formation of grain yield per unit area in both dry and wet years. In dry years, the correlation coefficient between yield and grain mass per plant was on average r = 0.80; in favorable years, r = 0.69. The relationship between yield and grain mass from the ear was greater — r = 0.84 and r = 0.82, respectively. Consequently, the breeding significance of the aboveground mass and the productivity of the ear, as a criterion for the selection of the crop, especially increases in the dry years. They were basic in the selection.

scholarly journals Development and Relative Validity of a Semiquantitative Food Frequency Questionnaire to Estimate Dietary Intake among a Multi-Ethnic Population in the Malaysian Cohort Project

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1163
Author(s):  
Suzana Shahar ◽  
Mohd Razif Shahril ◽  
Noraidatulakma Abdullah ◽  
Boekhtiar Borhanuddin ◽  
Mohd Arman Kamaruddin ◽  
...  
Keyword(s):  
Vitamin C ◽  
Correlation Coefficient ◽  
Food Frequency Questionnaire ◽  
Intraclass Correlation ◽  
Correlation Coefficients ◽  
Weighted Kappa ◽  
Dietary Intakes ◽  
Food Frequency ◽  
Relative Validity ◽  
Spearman’S Correlation

Measuring dietary intakes in a multi-ethnic and multicultural setting, such as Malaysia, remains a challenge due to its diversity. This study aims to develop and evaluate the relative validity of an interviewer-administered food frequency questionnaire (FFQ) in assessing the habitual dietary exposure of The Malaysian Cohort (TMC) participants. We developed a nutrient database (with 203 items) based on various food consumption tables, and 803 participants were involved in this study. The output of the FFQ was then validated against three-day 24-h dietary recalls (n = 64). We assessed the relative validity and its agreement using various methods, such as Spearman’s correlation, weighed Kappa, intraclass correlation coefficient (ICC), and Bland–Altman analysis. Spearman’s correlation coefficient ranged from 0.24 (vitamin C) to 0.46 (carbohydrate), and almost all nutrients had correlation coefficients above 0.3, except for vitamin C and sodium. Intraclass correlation coefficients ranged from −0.01 (calcium) to 0.59 (carbohydrates), and weighted Kappa exceeded 0.4 for 50% of nutrients. In short, TMC’s FFQ appears to have good relative validity for the assessment of nutrient intake among its participants, as compared to the three-day 24-h dietary recalls. However, estimates for iron, vitamin A, and vitamin C should be interpreted with caution.

Comparison of visual (MUNSELL) and instrumental measures (CIELAB) of coloured textile standard samples

2019 ◽  
Vol 23 (4) ◽  
pp. 340-354
Author(s):  
Asim Kumar Roy Choudhury ◽  
Biswajit Naskar
Keyword(s):  
Correlation Coefficient ◽  
Design Methodology ◽  
Correlation Coefficients ◽  
Standard Samples ◽  
Content Type ◽  
Psychophysical Data ◽  
Physical Data ◽  
Hue Angle ◽  
Munsell Scale

Purpose This paper aims to compare visual (Munsell) and instrumental (CIELAB) attributes of SCOTDIC colour standards. Design/methodology/approach SCOTDIC cotton and polyester standards of defined hue, value and chroma were subjected to spectrophotometric assessment for finding the corresponding instrumental parameters. The visual and instrumental parameters were compared. Findings The correlation between SCOTDIC value and CIELAB lightness is quite high. Correlation coefficient between SCOTDIC hue and CIELAB hue angle and the correlation between SCOTDIC chroma and CIELAB chroma were only moderate because the CIELAB chroma varied widely at higher chroma. When the standards of SCOTDIC hues having erratic hue angles at two extremes are excluded, the Correlation coefficients between SCOTDIC hue and CIELAB hue angle become high. Research limitations/implications The psychophysical data (visual) are difficult to match with physical data (instrumental). Originality/value The object of the present research is to study and compare visual (Munsell) and instrumental (CIELAB) colorimetric parameters. Munsell scale is physically exemplified by SCOTDIC fabric samples available in two sets, namely, cotton and polyester sets.

Effect of Surface Roughness on Slip Resistance of Rubber

2011 ◽  
Vol 189-193 ◽  
pp. 1538-1542
Author(s):  
Li Xiao Jia ◽  
Yong Zhen Zhang ◽  
Yong Ping Niu ◽  
San Ming Du ◽  
Jian Li
Keyword(s):  
Surface Roughness ◽  
Correlation Coefficient ◽  
Correlation Coefficients ◽  
Roughness Parameters ◽  
Test Conditions ◽  
Slip Resistance ◽  
Materials Used ◽  
Almost All ◽  
Effect Of Surface

In order to decrease accidents of slips and falls, COFs of rubber samples with different surface roughness were measured by Brungraber Mark II. And the correlation coefficients between roughness parameters and COF were calculated. The rusults have shown that the COF increases with surface roughness and the correlation coefficient between Sq and COF is highest. In general, almost all the roughness parameters used in the study have high correlation with COF. Parameters had the highest correlation with COF depends on the materials used and test conditions.

scholarly journals Innovative Correlation Coefficient Measurement with Fuzzy Data

2016 ◽  
Vol 2016 ◽  
pp. 1-11
Author(s):  
Berlin Wu ◽  
Chin Feng Hung
Keyword(s):  
Mathematics Achievement ◽  
Correlation Coefficient ◽  
Empirical Studies ◽  
Correlation Coefficients ◽  
Interval Data ◽  
Fuzzy Data ◽  
Pearson’S Correlation Coefficient ◽  
Coefficient Measurement ◽  
The Relationship ◽  
Fuzzy Interval

Correlation coefficients are commonly found with crisp data. In this paper, we use Pearson’s correlation coefficient and propose a method for evaluating correlation coefficients for fuzzy interval data. Our empirical studies involve the relationship between mathematics achievement and other projects.

scholarly journals Psychometric Properties of the MyotonPRO in Dementia Patients with Paratonia

Gerontology ◽  
2017 ◽  
Vol 64 (4) ◽  
pp. 401-412 ◽  
Author(s):  
Hans Drenth ◽  
Sytse U. Zuidema ◽  
Wim P. Krijnen ◽  
Ivan Bautmans ◽  
Cees van der Schans ◽  
...  
Keyword(s):  
Psychometric Properties ◽  
Correlation Coefficient ◽  
Intraclass Correlation ◽  
Correlation Coefficients ◽  
Functional Mobility ◽  
Future Research ◽  
Minimal Detectable Change ◽  
Muscle Properties ◽  
Dementia Patients ◽  
Longitudinal Outcome

Background: Paratonia is a distinctive form of hypertonia, causing loss of functional mobility in early stages of dementia to severe high muscle tone and pain in the late stages. For assessing and evaluating therapeutic interventions, objective instruments are required. Objective: Determine the psychometric properties of the MyotonPRO, a portable device that objectively measures muscle properties, in dementia patients with paratonia. Methods: Muscle properties were assessed with the MyotonPRO by 2 assessors within one session and repeated by the main researcher after 30 min and again after 6 months. Receiver operating characteristic curves were constructed for all MyotonPRO outcomes to discriminate between participants with (n = 70) and without paratonia (n = 82). In the participants with paratonia, correlation coefficients were established between the MyotonPRO outcomes and the Modified Ashworth Scale for paratonia (MAS-P) and muscle palpation. In participants with paratonia, reliability (intraclass correlation coefficient) and agreement values (standard error of measurement and minimal detectable change) were established. Longitudinal outcome from participants with paratonia throughout the study (n = 48) was used to establish the sensitivity for change (correlation coefficient) and responsiveness (minimal clinical important difference). Results: Included were 152 participants with dementia (mean [standard deviation] age of 83.5 [98.2]). The area under the curve ranged from 0.60 to 0.67 indicating the MyotonPRO is able to differentiate between participants with and without paratonia. The MyotonPRO explained 10-18% of the MAS-P score and 8-14% of the palpation score. Interclass correlation coefficients for interrater reliability ranged from 0.57 to 0.75 and from 0.54 to 0.71 for intrarater. The best agreement values were found for tone, elasticity, and stiffness. The change between baseline and 6 months in the MyotonPRO outcomes explained 8-13% of the change in the MAS-P scores. The minimal clinically important difference values were all smaller than the measurement error. Conclusion: The MyotonPRO is potentially applicable for cross-sectional studies between groups of paratonia patients and appears less suitable to measure intraindividual changes in paratonia. Because of the inherent variability in movement resistance in paratonia, the outcomes from the MyotonPRO should be interpreted with care; therefore, future research should focus on additional guidelines to increase the clinical interpretation and improving reproducibility.

scholarly journals AB0798 IS THERE A REPRODUCIBILITY OF THE HISTOGRAM AND GRAY SCALE IN ECOEXTRUCTURAL DAMAGES IN RHEUMATOTOLOGICAL DISEASES?

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1423.2-1424
Author(s):  
J. A. Mendonça ◽  
I. Siste de Almeida Aoki ◽  
C. C. Cavuto ◽  
V. A. Leandro-Merhi ◽  
J. L. B. D. Aquino
Keyword(s):  
Correlation Coefficient ◽  
Intraclass Correlation Coefficient ◽  
Rheumatic Diseases ◽  
Femoral Condyle ◽  
Intraclass Correlation ◽  
Correlation Coefficients ◽  
Concordance Correlation Coefficient ◽  
Image Method ◽  
Concordance Correlation ◽  
Gray Scale

Background:The gray scale (GS) in high resolution ultrasound is already well validated for use in rheumatological diseases, but the color map or the histogram, can be considered a new proposal, to better define and complement the echotextural damages detection1.Objectives:To calculate the lesions area measures reproducibility index in arthropathies, between 3 blind evaluators and correlate these measures using the GS and the histogram.Methods:Observational and retrospective study approved by the ethics committee of the Pontifical Catholic University of Campinas, with the opinion number: 1.526.307. A total of 29 patients have been assessed (31% males and 69% females) on period 2014 to 2019 in Rheumatology service. A MyLab 50 -Esaote equipment was used with frequency transducer that ranged between 6.0 and 18.0 MHz, 10 different area measures were performed from each recorded images previously, by the GS and the histogram. Statistical analysis: Spearman’s correlation coefficients, Lin’s concordance coefficient (CCC) and the intraclass correlation coefficient (ICC) and their respective 95% confidence intervals, with the SPSS software package for Windows v. 17.0 (SPSS Inc., Chicago, IL, USA).Results:Average age 43.5 ± 21.5 years of age; with disease duration that varied between ≤ 1 month (48.3%) and ≥36 months (24.1%); with the following diseases: juvenile idiopathic arthritis (17.24%); osteoarthritis (13.79%); psoriatic arthritis (13.79%); undifferentiated spondyloarthritis (3.44%); gout (20.68%); rheumatoid arthritis (27.58%) and reactive arthritis (3.44%). A total of 840 measures of exudative (27.58%), proliferative (27.58%) and snowstorm appearance (6.89%) synovitis were performed; femoral-condyle cartilage (3.44%); synovial cyst (3.44%); paratendinitis (6.89%); calcification (3.44%); nail enthesitis (3.44%); tenosynovitis (6.89%) and tophi (10.34%) (Figure 1). The concordance correlation coefficient showed values closer to 1; p <0.001, the intraclass correlation coefficients with excellent reproducibility (ICC ≥ 0.75); p <0.001, always in relation to the three evaluators (Table 1) and the Spearman correlation between the GS and the histogram ranged from rs = 0.665 to rs = 1,000; p <0.001.Conclusion:The histogram can be considered an image method to better identify echotextural damages.References:[1]Mendonça J, Provenza J, Guissa V, et al AB1059 2D Histogram Ultrasound and 3D Ultrasound Correlation in Rheumatic Diseases Annals of the Rheumatic Diseases 2015; 74:1253-1254.Table 1.Concordance Correlation Coefficient (CCC) and Intraclass Correlation Coefficient (ICC):EvaluatorsGS - CCC(IC 95%)p-valorHistogram (IC 95%)p-valor1 e 20,998(0,994-0,999)<0,0010,999(0,995-1,000)<0,0011 e 30,998(0,995-0,999)<0,0010,999(0,995-1,000)<0,0012 e 30,992(0,980-0,997)<0,010,996(0,979-0,999)<0,01Standard by US 2DICCp-valorGS0,997(0,992-0,999)<0,001Histogram0,998(0,992-0,999)<0,001Legends: Gray Scale (GS).Figure 1.Patient with gout: A and B: Tophi area measures (star) in right metatarsos and efusion (arrow) by GS (45 mm2) and histogram (39 mm2), respectively.Disclosure of Interests:José Alexandre Mendonça Speakers bureau: Novartis, Janssen, Bristol, UCB, Isabella Siste de Almeida Aoki: None declared, Caique Chagas Cavuto: None declared, Vânia Aparecida Leandro-Merhi: None declared, José Luis Braga de Aquino: None declared

scholarly journals Subclinical mastitis in dairy camels in Algeria: Comparison of screening tests

2017 ◽  
Vol 108 (2) ◽  
pp. 85
Author(s):  
Leyla HADEF ◽  
Hebib AGGAD ◽  
Brahim HAMAD ◽  
Mohamed Said MAHMOUD ◽  
Aicha ADAIKA
Keyword(s):  
Subclinical Mastitis ◽  
Screening Tests ◽  
Threshold Values ◽  
Bacteriological Culture ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Cultural Isolation ◽  
Kappa Value ◽  
Indirect Tests ◽  
Reliable Diagnostic Method

The aim of the present study was to determine a threshold values and to assess the effectiveness of four indirect tests for the diagnosis of subclinical mastitis in dairy camels comparing with bacteriological culture. One hundred fifty three milk samples from 17 lactating camels were subjected to bacteriological culture, where 84 milk samples were positive, 47 were negative and 22 samples were considered as contaminated. A total of 131 milk samples were screened by pH, electrical conductivity (EC), California mastitis test (CMT) and somatic cell count (SCC). The good combination of sensitivity and specificity were obtained with a threshold of 6.55, 7.2 mS/cm, score trace was considered as CMT (+) and 240 000 cells/ml for the four tests, respectively. The sensitivity of the SCC, pH, EC and CMT was 72.61, 66.66, 47.61 and 39.28 %; the specificity 70.21, 38.02, 59.57 and 72.34 %; percentage accuracy 71.75, 51.14, 51.90 and 51.14 %; and positive predictive value 81.33, 47.61, 67.79 and 71.73 %, respectively. The SCC was significantly correlated with bacteriological culture (r = 0.415, p &lt; 0.05). Kappa value of SCC was higher than that of other tests (SCC &gt; CMT &gt; EC &gt; pH). In conclusion, the results suggest that the SCC was the most accurate, reliable, diagnostic method compared to other tests used in this study after cultural isolation for the detection of subclinical mastitis in dairy camel under field conditions.

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