Positive associations between infections ofToxoplasma gondiiand seropositivity withAnisakis simplexin human patients suffering from chronic urticaria

2014 ◽  
Vol 89 (6) ◽  
pp. 707-713 ◽  
Author(s):  
V. Fernández-Fígares ◽  
M. Rodero ◽  
A. Valls ◽  
C. De Frutos ◽  
A. Daschner ◽  
...  
Keyword(s):  
Chronic Urticaria ◽  
Odds Ratio ◽  
Specific Ige ◽  
Anisakis Simplex ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Atopic Sensitization ◽  
Prick Test ◽  
Food Borne ◽  
The Relationship

AbstractToxoplasma gondiiis a food-borne and orofecal microorganism which produces chronic infection, and attempts have been made to prove its negative association with atopy in the context of the hygiene hypothesis.Anisakis simplexis a fish parasite associated with chronic urticaria (CU) in endemic regions. We analysed the relationship between both infectious agents in CU. We included 42 patients with chronic urticaria (18 patients with CU associated withA. simplexsensitization and 24 not sensitized CU patients). Patients were assessed for atopy by a skin prick test (SPT) against common aeroallergens and for respiratory symptoms.Anisakis simplexsensitization was assessed by SPT and specific IgE by CAP fluoro-enzyme immunoassay (CAP-FEIA). Anti-T. gondiiIgG levels were measured by enzyme-linked immunosorbent assay (ELISA). CU patients were analysed with respect toT. gondiiseropositivity,A. simplexsensitization, atopy and immigrant status. The seroprevalence ofT. gondiiwas 40.5% in CU patients and 42.1% in the control group. Immigrants were more frequently infected byT. gondii(41.2% versus 12%;P =0.036). Anti-T. gondiiIgG antibodies were associated with pastA. simplexparasitism (odds ratio 6.73;P =0.03) and independently with atopic sensitization (odds ratio 5.85;P =0.04). In CU patients,T. gondiihas no protective effect on atopic sensitization orA. simplexsensitization.

scholarly journals Evaluation of the Relationship between the Level of DHEA-S and Sex Hormones in Some Infertile Iraqi Men

2021 ◽  
Vol 8 (5) ◽  
pp. 130-136
Author(s):  
Khaled S. Abd ◽  
Adnan F. AL-Azzawie
Keyword(s):  
Sex Hormones ◽  
Seminal Fluid ◽  
Age Groups ◽  
Serum Levels ◽  
Dehydroepiandrosterone Sulfate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Infertile Men ◽  
The Relationship

This study aims to evaluate serum levels of the Dehydroepiandrosterone sulfate (DHEA-S) and its relationship with some sex hormones such as Follicle stimulating hormone (FSH), Luteinizing hormone (LH), prolactin and testosterone hormone and the infertility type in some infertile Iraqi men. Blood and seminal fluid samples from (60) idiopathic male infertile and (60) healthful individuals as a control group aged (18 to 60 year) were collected from private clinics. Serum hormones (DHEA-S, FSH, LH, prolactin and testosterone) were measurement using Enzyme Linked Immunosorbent Assay (ELISA). The levels of DHEA-S, FSH, LH and prolactin are significantly higher (P≤0.01) expect of the testosterone level was significantly decreased (P≤ 0.01) in the infertile men as compared with control group. High significant differences (P≤0.01) were recorded when comparing the hormonal levels (DHEA-S, FSH, LH, prolactin and testosterone) according to the age groups. There are significant differences (P≤ 0.01) in levels of (DHEA-S, FSH, LH, prolactin and testosterone) in the infertility period.  Smoker infertile men have high levels (P≤ 0.05) in the DHEA-S and LH while have low levels in the prolactin hormones compared with control. Patients with family history have shown significant differences (P≤0.05) in the levels of DHEA-S, FSH, LH and prolactin. In conclusion, this study revealed significantly increase in the DHEA-S levels in the infertile men and negative correlation between DHEA-S and FSH. Therefore, DHEA-S has important role in the diagnosis and follow up of the male infertility.

scholarly journals Urine CA-2 as a biomarker for diagnosis urinary stone and prediction of its complications

2020 ◽  
Author(s):  
Yuan-jing Leng ◽  
Hai-bin Zhou ◽  
Jiang-ling Fu ◽  
Wen-juan Wang
Keyword(s):  
Healthy Subjects ◽  
Characteristic Curve ◽  
Urinary Stone ◽  
Urinary Stones ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Control ◽  
The Mean ◽  
The Difference ◽  
The Relationship

Abstract PURPOSECarbonic anhydrase-2 (CA-2) plays a role in mineralization and calcification in organism. Strong evidence suggests that CA-2 is associated with urolithiasis. However, the relationship between CA-2 and urinary stone remains unclear. The study aimed to assess the association of urine CA-2 (uCA-2) level and the potential risk of urinary stone.METHODSFrom March 2017 to November 2019, a prospective cohort study was conducted on patients with urinary stones and healthy subjects to determine the pretreatment uCA-2 level detection by Enzyme linked immunosorbent assay (ELISA). The difference of uCA-2 level between patients with urinary stones and healthy subjects was compared. Then comparison between stone patients with complications and without complications was carried out as well as correlation analysis to detect factors associated with biomarker expression.RESULTS118 patients with urinary stones were into urinary stones group and 42 healthy subjects were into healthy control group. The mean pretreatment uCA-2 level was significantly higher in patients with urinary stones group than healthy controls group (P=0.028). Furthermore, The uCA-2 level was positive correlation with urinary stones complications (R=0.379, P=0.000), especially pain complications (R=0.524, P=0.000) and hematuria complications (R=0.374, P=0.000). Receiver operating characteristic curve (ROC) analysis that a uCA-2 level threshold of 10.94 ng/mL had 83.67% sensitivity and 68.12% specificity for predicting urinary stones complications. CONCLUSIONSExcessive uCA-2 excretion is a major risk factor for urinary stone. Our findings suggested that uCA-2 may be used as an unappreciated biomarker for the diagnosis urinary stone in patients and to predict its complications.

scholarly journals The expression and significance of inflammatory cytokines and MMP-9 in the tears of the infected eye and contralateral uninfected eye in patients with fungal keratitis

2021 ◽  
Author(s):  
Shuang Zhang ◽  
Hui-Min Wu ◽  
Xiang-Ni Cao ◽  
Xian-Qi Zhang ◽  
Gui-ping Gao
Keyword(s):  
Tear Film ◽  
Fungal Keratitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Healthy Controls ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Healthy Control ◽  
The Relationship ◽  
Tear Film Function

Abstract Background: We investigated bilateral tear cytokine levels including interleukin (IL)-1β, IL-10, IL-17, tumor necrosis factor (TNF)-α and Matrix metalloproteinase-9 (MMP-9) in patients with fungal keratitis(FK). Meanwhile, we evaluated the relationship between the changes of tear cytokines with corneal perception and pain in infected eyes, and the relationship between tear cytokines and tear film function in contralateral uninfected eyes .Methods : A total of 60(20 FK, 20 contralateral, 20 healthy controls) tear samples were collected prospectively and analyzed by enzyme linked immunosorbent assay(ELISA). Approximately 50 to 60 ul of tear samples in each case were collected. Meanwhile ,we analyzed the changes of visual analogue scale(VAS), tear breakup time (TBUT), Schirmer I test (SIT) and corneal perception compared with healthy controls. Results :The concentrations of IL-1β, IL-10 and IL-17 increased in bilateral eyes compared with healthy controls(P<0.05). The tear concentrations of MMP-9 , TNF-α only significantly increased in affected eyes (P <0.05). Patients with FK showed significant reduction in corneal perception of infected eyes compared with controls(P<0.05). Corneal perception of the normal eyes in FK patients was slightly lower than that of control group, but there was not statistical difference (P>0.05).TBUT and SIT of contralateral uninfected eyes were significantly lower than that of control group(P<0.05), which were significantly correlated with levels of IL-1β, IL-17(P<0.05). SIT were also negatively correlated with MMP-9(P<0.05), while the levels of IL-1β, IL-10, IL-17, TNF-α and MMP-9 in the tears of the healthy control group had no significant correlation with TBUT and SIT indicators(P>0.05).The corneal perception and VAS score of the affected FK eyes showed correlation with IL-1β, IL-17 and TNF-α(P<0.05).In addition, concentration of IL-10 inversely was correlated with VAS (P<0.05). Conclusion: Proinflammatory tear cytokines are elevated in bilateral eyes with unilateral FK as associated with tear film function ,pain and corneal sensitivity.

Enzyme-linked immunosorbent assay, immunoblot analysis and RAST fluoroimmunoassay analysis of serum responses against crude larval antigens of Anisakis simplex in a Spanish random population

1996 ◽  
Vol 70 (4) ◽  
pp. 281-289 ◽  
Author(s):  
L. García-Palacios ◽  
M.L. González ◽  
M.I. Esteban ◽  
E. Mirabent ◽  
M.J. Perteguer ◽  
...  
Keyword(s):  
Optical Density ◽  
Immunoblot Analysis ◽  
Specific Ige ◽  
Negative Control ◽  
Anisakis Simplex ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Sera ◽  
Specific Ige Antibodies ◽  
Reference Serum ◽  
Diagnostic Criterion

AbstractThe results obtained in a study of seroprevalence by means of ELISA and immunoblot with crude larval extracts of Anisakis simplex using 1008 human sera from Spanish people showing no clinical suspicion of anisakidosis are given. For the evaluation of the results obtained by ELISA the Diagnostic Index (DI) was used, as the ratio between the optical density resulting from the test serum and the optical density of the negative control. Forty-seven sera showed DIs between 1.5 and 2, and 14 sera were greater than 2. After comparison of the immunoblot analysis with the immunorecognition pattern of a human anisakidosis reference serum, a diagnostic criterion could be established for those sera that, at a 1/100 dilution, showed a DI by ELISA greater than 1.5. Seven of 14 selected sera with DIs in ELISA higher than 1.3 showed anti-Anisakis specific IgE antibodies by RAST fluoroimmunoassay.

scholarly journals Prognostic role of metabolic risk factors (hyperhomocysteinemia) in the development of chronic heart failure

2015 ◽  
Vol 18 (1) ◽  
pp. 20
Author(s):  
Ye. N. Berezikova ◽  
M. G. Pustovetova ◽  
S. N. Shilov ◽  
A. V. Yefremov ◽  
A. T. Teplyakov ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Blood Serum ◽  
Homocysteine Level ◽  
Functional Class ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Relationship Of ◽  
The Relationship

The aim of the study was to assess the relationship of homocysteine levels in the blood serum with the severity and nature of chronic heart failure (CHF) in patients with coronary heart disease. 94 patients with CHF were examined. The control group included 32 patients without cardiovascular disorders. At baseline and after 12 months of observation the homocysteine levels in the blood serum were determined by enzyme-linked immunosorbent assay. Correlative relationship of hyperhomocysteinemia with ischemic myocardial remodeling and with reduced inotropic function in CHF patients was observed. The homocysteine level in the blood serum of CHF patients significantly exceeded that of the control group and moderately increased with the progression of the disease severity of functional class. In the group with an unfavorable course of CHF the baseline homocysteine level proved to have the greatest value as compared to that in the group with a favorable course. In patients with a favorable course of CHF the homocysteinemia level tended to decrease towards the end of prospective study, whereas in patients with an unfavorable course, on the contrary, hyperhomocysteinemia persisted (p<0.01). In case the hyperhomocysteinemia baseline exceeds 18.5 mkmol/L (ROC-AreaSE = 0.860.04, sensitivity 71 %, specificity 90%), the severity and characteristics of the ischemic CHF could be most likely predicted. Thus, hyperhomocysteinemia is related with the severity and nature of CHF. Determining the level of homocysteine in the blood serum can be recommended for early prediction of the severity and nature of CHF.

scholarly journals The Relationship Between MMP-2 -1306C>T and MMP-9 -1562C>T Polymorphisms and the Risk and Prognosis of T-Cell Acute Lymphoblastic Leukemia in a Chinese Population: A Case-Control Study

2017 ◽  
Vol 42 (4) ◽  
pp. 1458-1468 ◽  
Author(s):  
Cong-Meng Lin ◽  
Yan-Ling Zeng ◽  
Min Xiao ◽  
Xu-Qiao Mei ◽  
Lv-Ying Shen ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Cox Model ◽  
Lymphoblastic Leukemia ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Case Group ◽  
Kaplan Meier ◽  
Cell Acute Lymphoblastic Leukemia ◽  
The Relationship

Background: T-cell acute lymphoblastic leukemia (T-ALL) is a malignant hematological disease and is often accompanied by a variety of genetic abnormalities. Hence, our study aims to investigate the relationship between MMP-2 -1306C>T and MMP-9 -1562C>T polymorphisms and the risk and prognosis of T-ALL. Methods: From April 2009 to February 2011, a total of 376 T-ALL patients were chosen as the case group. Meanwhile, 352 healthy people who passed routine health examinations were selected as the control group. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to detect the frequency of MMP-2 -1306C>T (rs243865) and MMP-9 -1562C>T (rs3918242) polymorphisms in the study subjects. The serum levels of MMP-2 and MMP-9 were detected using enzyme-linked immunosorbent assay (ELISA). A Kaplan-Meier analysis was employed to analyze the event-free survival (EFS) rates of the T-All patients with different MMP-2 and MMP-9 genotypes. A multivariate COX model was applied to analyze the relationship between MMP-2 and MMP-9 polymorphisms and the prognosis of T-ALL patients. A C-statistic and net reclassification index (NRI) was carried out to evaluate the predictive value of MMP-2 and MMP-9 gene polymorphisms using the Cox model. Results: Compared to the control group, the genotypic frequency of MMP-2 -1306C>T (CT + TT) and MMP-9 -1562C>T (CT + TT) in the case group was significantly higher. The serum level of MMP-9 was markedly elevated in T-ALL patients with the CT + TT genotype compared to patients with the CC genotype. The results of the Kaplan-Meier analysis showed that the median EFS was lower in T-ALL patients with the CT + TT genotype of MMP-9 -1562C>T compared to patients with the CC genotype. The results of a multivariate analysis using the Cox proportional hazard model indicated that the MMP-9 -1562C>T polymorphism was associated with the prognosis of T-ALL patients. Conclusion: These results indicated that MMP-2 -1306C/T and MMP-9 -1562C/T polymorphisms might be associated with an increased risk of T-ALL. The MMP-9 -1562C>T polymorphism may also be related to the prognosis of T-ALL patients.

scholarly journals Clinical and immunological characterization of perilla seed allergy in children

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
J Kyunguk ◽  
L So-Yeon ◽  
J Se-Ah ◽  
G Purevsan ◽  
N Jin Young ◽  
...  
Keyword(s):  
Case Reports ◽  
Specific Ige ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Composition Analysis ◽  
Prick Test ◽  
Molecular Weights ◽  
Liquid Chromatography Tandem Mass ◽  
Tertiary Hospitals ◽  
Perilla Seed

Background: Perilla seeds are known to cause immediate allergic reactions. However, reports on perilla seed allergies are limited to a few case reports worldwide, and there is currently no diagnostic test for such allergies. Objective: Our objective was to analyze the clinical and immunological characteristics of perilla seed allergy and to identify allergens for the development of diagnostic methods. Methods: Twenty-one children with clinical perilla seed allergy were enrolled from two tertiary hospitals between September 2016 and June 2019. Using perilla seed extract, we developed a skin prick test (SPT) reagent and an IgE enzyme-linked immunosorbent assay (ELISA) for perilla seed allergy diagnosis. IgE immunoblotting was performed for identifying putative allergenic components, and amino acid composition analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: The median age of children with perilla seed allergy was 3 years; the proportion of children with anaphylaxis was 28.6%. Perilla seed SPT was performed in 15 of 21 children, all of whom tested positive. On ELISA, 85.7% of children tested positive for perilla seed-specific IgE. Proteins with molecular weights of 50, 31–35, and 14–16 kDa showed binding with the sera of >50% of children with perilla seed allergy. LC-MS/MS analysis of these three protein fractions indicated 8 putative proteins, including perilla oleosin (Accession No. 9963891), to be allergens. Conclusion: This study documented the clinical characteristics and immunological profiles of 21 children with perilla seed allergy. Our results suggest that oleosin is one of the major allergens in perilla seeds.

scholarly journals INFLUENCE OF MARKERS OF ENERGETIC HOMEOSTASIS AND ADIPOKINES ON THE REGULATION OF CARBOHYDRATE METABOLISM IN PATIENTS WITH ACUTE MYOCARDIAL INFARCTION AND CONCOMITANT OBESITY

2020 ◽  
Vol 20 (4) ◽  
pp. 62-67
Author(s):  
M.Yu. Koteliukh
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Carbohydrate Metabolism ◽  
Energy Homeostasis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Regulation Of Carbohydrate Metabolism ◽  
Microsoft Office ◽  
The Relationship

There have been reports on the effect of energy homeostasis markers and adipokines (FABP 4 and CTRP 3) on the development and course of cardiovascular disease in patients with concomitant obesity. The role of energy homeostasis and adipokines in the development of acute myocardial infarction and concomitant diseases is still insufficiently studied. The purpose of this work was to study the relationship between markers of energy homeostasis, adipokines and carbohydrate metabolism in patients with acute myocardial infarction and syntropic pathology. The study included 189 patients with acute myocardial infarction with and without obesity. The control group included 20 healthy individuals. Adropin, irisin, FABP 4, CTRP 3, insulin were determined by enzyme-linked immunosorbent assay. Mathematical computer processing of the findings obtained were performed using the software package "IBM SPPS Statistics 27.0" and Microsoft Office Excel. All groups of the patients were found to have increased levels of glucose, insulin, HOMA index compared with the control group (p˂0.05). The patients of all group demonstrated a decrease in adropine, irisin and CTRP 3 and an increase in FABP 4 when compared with the control group (p˂0.05). Analysis of the results points out the correlation between carbohydrate metabolism and the level of adropine, irisin, FABP 4, and CTRP 3 in all groups of the patients. Thus, the study has shown the influence of energy metabolism and adipokine system on carbohydrate metabolism in the patients with acute myocardial infarction depending on the presence and absence of obesity.

scholarly journals The association between lichen planus and hepatitis C virus infection: A case control study

2021 ◽  
Vol 12 (e) ◽  
pp. e85-e85
Author(s):  
G Rai Subodha Kumar ◽  
Nagesha Parvathi ◽  
RangeGowda Suresh ◽  
Rangaswamy Umadevi ◽  
Nirvanappa Vinay
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Lichen Planus ◽  
Skin Diseases ◽  
Hcv Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
P Value ◽  
Case Group ◽  
The Relationship

Background: The incidence of Hepatitis C virus (HCV) infection among patients with Lichen planus (LP) varies considerably. There is also lack of data in Indian population. Due to these variation, this study is to assessing the prevalence of HCV infection among patients with CLP in Hassan Karnataka. Methods: 135 clinically diagnosed cases of Lichen planus more than 18 years of age will be included as cases.147 patients having skin diseases other than lichen planus will be included as controls. Details of the patients personal history and family history,medical history and blood transfusion history will be collected. Detection of antibodies against HCV (anti-HCV) by enzyme-linked immunosorbent assay (ELISA). Results: Anti-HCV antibodies were found in 3(2.2%) patients of the case group and 1(0.7%) in control group. P value is 0.274, not statistically significant. Conclusion: Currently, there is no evidence confirming the relationship between HCV infection and CLP in Hassan Karnataka. However, there is a need for further research on larger groups of

scholarly journals Serum Neutrophıl Gelatınase-Assocıated Lıpocalın Levels in Patients with Non-Dipper Hypertension

2015 ◽  
Vol 38 (1) ◽  
pp. 53 ◽  
Author(s):  
Gökhan Aksan ◽  
Sinan İnci ◽  
Gökay Nar ◽  
Serhat Siğirci ◽  
Ömer Gedikli ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Blood Pressure Monitoring ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Pressure Monitoring ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Patient Groups ◽  
And Gender ◽  
Neutrophil Gelatinase ◽  
The Relationship

Purpose: Neutrophil gelatinase-associated lipocalin (NGAL) is a protein belonging to the lipocalin superfamily and plays a role in atherosclerosis, renal injury and inflammation. The present study aimed to investigate serum NGAL concentrations in groups of patients with dipper and non-dipper hypertension (HT) and to characterize the relationship between NGAL concentration and circadian blood pressure in hypertensive patients. Methods: A total of 41 (22 male, 19 female, mean age: 56.1 ±8.9 years) non-dipper HT patients, 40 (19 male, 21 female, mean age: 54.0 ±10.0 years) dipper HT patients and 42 age- and gender-matched healthy individuals were enrolled in the study. Dipper and non-dipper HT were diagnosed via ambulatory blood pressure monitoring. Serum NGAL concentrations were measured by enzyme-linked immunosorbent assay from blood samples obtained from patients. Results: Serum NGAL concentrations were found to be significantly higher in the non-dipper and dipper HT patient groups in comparison with the control group (84.9 ±23.0 ng/ml and 62.1 ±17.8 vs. 46.6 ± 13.7 ng/ml, p

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