Effect of Simazine on Photosynthesis and Growth of Filamentous Algae

Weed Science ◽  
1983 ◽  
Vol 31 (6) ◽  
pp. 899-903 ◽  
Author(s):  
Steven W. O'Neal ◽  
Carole A. Lembi

Simazine [2-chloro-4,6-bis(ethylamino)-s-triazine] inhibited photosynthesis 50% at concentrations of 1.1 μM forSpirogyra jurgensii(Kutz), 3.0 μM forPithophora oedogonia(Mont.) Wittr., and 3.8 μM forCladophora glomerata(L.) Kutz. Photosynthesis ofAnkistrodesmus braunii(Brun.), a nonfilamentous species, was inhibited the same amount by 4.7 μM simazine. The filamentous algal species had significant reductions in growth but no other phytotoxic symptoms when exposed to 5 μM simazine at light intensities of 100 μE·m–2·s–1and below. Algicidal effects did occur at a light intensity of 400 μE·m–2·s–1and were most severe inSpirogyra.The relationship between light intensity and simazine toxicity indicates algicidal effectiveness on these filamentous algae will be limited in habitats where light is reduced by turbidity, depth, or self-shading.

Author(s):  
. Guntur ◽  
. Fuad ◽  
Ali Muntaha

<p>ABSTRACT<br /><br />Purpose of the study is to examine the effect of underwater lamp intensity on the fishing catches of the lift net. A series of the experiment was conducted to test the most effective underwater lamp intensity to aggregate school of fish for lift net fishing target. Four underwater light intensities, such as 140 lux (kerosene lamp) as a control, 290 lux, 2700 lux, and 3900 lux were use as the treatment in this experiments. All the variance data of lift net’s fishing catches were analyzed by ANOVA followed LSD 5% to get the best treatment. The results show that the intensity of underwater lamp was significantly (P&lt;0.05) on the fishing catches of the lift net. The highest fishing catches was underwater lamp with 3900 lux light intensity as 254 kg fishing catches. While the underwater lamp with 2700 lux was 149,5 kg, underwater with 290 lux was 146 kg, and underwater lamp with 140 lux was 91.5 kg. The composition of the fishing catches during experiment were anchovy (Stelophorus sp) as 35.28%, yellow strip (Selaroides sp) as 33.59%, and ponyfish (Leiognathus sp) as 11:25%. The relationship between light intensity on the number and composition are discussed details in the paper.<br /><br />Keywords: lift net, light intensity, underwater lamp</p><p>-------<br /><br />ABSTRAK<br /><br />Penelitian ini adalah bertujuan untuk mendapatkan intensitas lampu bawah air yang terbaik pada bagan tancap yang beroperasi di perairan Selat Madura. Metode yang digunakan adalah metode eksperimen yang menggunakan empat perlakuan, yaitu lampu bawah air yang memiliki intensitas 140 lux (lampu petromax) sebagai perlakuan 1, intensitas 290 sebagai perlakuan 2, intensitas 2700 lux sebagai perlakuan 3 dan intensitas 3900 lux sebagai perlakuan 4. Analisa data yang digunakan adalah Anova yang dilanjutkan uji BNT 5% untuk mendapatkan perlakuan terbaik. Berdasarkan hasil pengamatan menunjukkan bahwa masing–masing perlakuan menghasilkan tangkapan yang berbeda-beda. Jumlah tangkapan perlakuan 1 (lampu 140 lux) sebesar 91,5 kg, perlakuan 2 (lampu 290 lux) 146 kg, perlakuan 3 (lampu 2700 lux) 149,5 kg, dan perlakuan 4 (lampu 3900 lux) 254 kg. Hasil perhitungan anova menunjukkan bahwa intensitas memberikan pengaruh sebesar 73% terhadap hasil tangkapan ikan. Perlakuan 1 (140 lux) dan perlakuan 2 (290 lux) berbeda nyata terhadap perlakuan 4 (3900 lux) tetapi tidak berbeda nyata terhadap perlakuan 3 (2700 lux). Intensitas cahaya lampu terbaik adalah perlakukan 4 (3900 lux) dengan hasil tangkapan ikan sekitar 254 kg dan belum mencapai titik maksimum. Komposisi hasil tangkapan di bagan tancap didominasi oleh jenis ikan teri (Stelophorus sp) sebesar 35,28 %, ikan selar (Selaroides sp) sebesar 33,59 %, ikan peperek (Leiognathus sp ) sebesar 11,25 %.<br /><br />Kata kunci: bagan tancap, intensitas cahaya, lampu bawah air</p>


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jingying Zhang ◽  
Changhai Sui ◽  
Huimin Liu ◽  
Jinjiao Chen ◽  
Zhilin Han ◽  
...  

Abstract Background ‘Regal Splendour’ (Hosta variety) is famous for its multi-color leaves, which are useful resources for exploring chloroplast development and color changes. The expressions of chlorophyll biosynthesis-related genes (HrHEMA, HrPOR and HrCAO) in Hosta have been demonstrated to be associated with leaf color. Herein, we isolated, sequenced, and analyzed HrHEMA, HrPOR and HrCAO genes. Subcellular localization was also performed to determine the location of the corresponding enzymes. After plasmid construction, virus-induced gene silencing (VIGS) was carried out to reduce the expressions of those genes. In addition, HrHEMA-, HrPOR- and HrCAO-overexpressing tobacco plants were made to verify the genes function. Changes of transgenic tobacco were recorded under 2000 lx, 6000 lx and 10,000 lx light intensity. Additionally, the contents of enzyme 5-aminolevulinic acid (5-ALA), porphobilinogen (PBG), chlorophyll a and b (Chla and Chlb), carotenoid (Cxc), superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA), proline (Pro) and catalase (CAT) under different light intensities were evaluated. Results The silencing of HrHEMA, HrPOR and HrCAO genes can induce leaf yellowing and chloroplast structure changes in Hosta. Specifically, leaves of Hosta with HrCAO silencing were the most affected, while those with HrPOR silencing were the least affected. Moreover, all three genes in tobacco were highly expressed, whereas no expression was detected in wild-type (WT). However, the sensitivities of the three genes to different light intensities were different. The highest expression level of HrHEMA and HrPOR was detected under 10,000 lx of illumination, while HrCAO showed the highest expression level under 6000 lx. Lastly, the 5-ALA, Chla, Cxc, SOD, POD, MDA, Pro and CAT contents in different transgenic tobaccos changed significantly under different light intensities. Conclusion The overexpression of these three genes in tobacco enhanced photosynthesis by accumulating chlorophyll content, but the influential level varied under different light intensities. Furthermore, HrHEMA-, HrPOR- and HrCAO- overexpressing in tobacco can enhance the antioxidant capacity of plants to cope with stress under higher light intensity. However, under lower light intensity, the antioxidant capacity was declined in HrHEMA-, HrPOR- and HrCAO- overexpressing tobaccos.


In a tank filled with a suspension of indian ink in tap water, a population of Daphnia magna will undergo a complete cycle of vertical migration when an overhead light source is cycli­cally varied in intensity. A ‘dawn rise’ to the surface at low intensity is followed by the descent of the animals to a characteristic maximum depth. The animals rise to the surface again as the light decreases, and finally show a typical midnight sinking. The light intensities at the level of the animals in this experiment are of the same order as those which have been reported in field observations; the time course of the movement also repeats the natural conditions in the field. The process is independent of the duration of the cycle and is related only to the variation in overhead light intensity. At low light intensity the movement of the animal is determined solely by positive photo-kinesis; the dawn rise is a manifestation of this, and is independent of the direction of the light. At high light intensities there is an orientation response which is superimposed upon an alternating positive (photokinetic) phase and a negative phase during which movement is inhibited. The fully oriented animal shows a special type of positive and negative phototaxis, moving towards the light at reduced light intensities and away from it when the light intensity is increased. In this condition it follows a zone of optimum light intensity with some exactness. Experiments show that an animal in this fully oriented condition will respond to the slow changes of intensity characteristic of the diurnal cycle, while being little affected by tran­sient changes of considerable magnitude.


1969 ◽  
Vol 47 (7) ◽  
pp. 1153-1156 ◽  
Author(s):  
J. A. Ekundayo ◽  
R. H. Haskins

Cultures of Botryodiplodia theobromae Pat. produced pycnidia abundantly on several media under continuous irradiation with fluorescent light. The fungus did not sporulate when grown in darkness. Irradiation of cultures with a light intensity of 15 foot-candles for 4 days was sufficient to stimulate pycnidial production, but for appreciable sporulation to occur over the same exposure period, higher light intensities are required. Irradiation of cultures through glass color filters showed that long-wave ultraviolet radiation stimulated sporulation.


1982 ◽  
Vol 54 (1) ◽  
pp. 329-339
Author(s):  
ELIZABETH SMITH ◽  
PAUL R. FISHER ◽  
WARWICK N. GRANT ◽  
KEITH L. WILLIAMS

The speed of sustained migration of Dictyostelium discoideum slugs was similar in a temperature gradient and at different light intensities, including a light intensity sufficient to cause significant disorientation of slugs. No change was observed in slug speed in the presence of high levels of Slug Turning Factor (STF), a low molecular weight compound through which phototaxis and thermotaxis are mediated. Thus orientation of D. discoideum slugs is not mediated by a sustained changed in slug speed and we propose that slug movement is not directly coupled to tactic responses. Slug speed depended on the size, age and genotype of slugs as well as the nature of the substratum (charcoal-containing water agar versus water agar).


2012 ◽  
Vol 72 (2) ◽  
pp. 343-351 ◽  
Author(s):  
MC. Bittencourt-Oliveira ◽  
B. Buch ◽  
TC. Hereman ◽  
JDT. Arruda-Neto ◽  
AN. Moura ◽  
...  

Cylindrospermopsis raciborskii (Woloszynska) Seenayya et Subba Raju (Ordem Nostocales) is one of the most troublesome bloom-forming species in Brazil. Understanding the population dynamics of the different morphotypes of C. raciborskii (straight and coiled) could assist in the prediction of favourable conditions for the proliferation of this potentially toxin-producing species. The aim of the present study was to assess the effects of two different light intensities and temperatures on the growth rate and morphology of the trichomes of the straight and coiled morphotypes. For such, two non-toxin producing strains of C. raciborskii were used - one with a coiled trichome (ITEP31) and another with a straight trichome (ITEP28). The strains were cultured in BG-11 medium in a climatic chamber under controlled conditions. Two light intensities (30 and 90 µmol.m-2.s-1 ) were combined at temperatures of 21 and 31 °C and the growth rate and morphological changes were analysed. The morphotypes responded differently to the different temperatures and light intensities. Both strains exhibited faster growth velocities when submitted to higher light intensity and temperature. The lower temperature and higher luminosity hampered the development of both strains. Variations in cellular morphology and an absence of akinetes in both strains were related to the lower temperature (21 °C). The coiled morphotype demonstrated considerable phenotype plasticity, changing the morphology of trichome throughout its growth curve. Although molecular analysis does not sustain the separation of the morphotypes as distinct species, their different eco-physiological responses should be considered further knowledge of extreme importance for the population control of these potentially toxic organisms.


1955 ◽  
Vol 33 (5) ◽  
pp. 821-829 ◽  
Author(s):  
Moyra J. Smith ◽  
Patricia M. Beatty ◽  
J. A. Pinder ◽  
D. J. Le Roy

The mercury (3P1) photosensitized hydrogenation of ethylene has been studied at room temperature as a function of ethylene concentration, mercury concentration, and light intensity. In addition to combination and disproportionation, ethyl radicals have been shown to take part in the reaction[Formula: see text]The conditions favoring this reaction have been established and anomalous values previously found for the ratio of ethane to butane have been explained. The value obtained for the ratio of the rate constants for the disproportionation and combination of ethyl radicals, 0.15 ±.01, is in excellent agreement with the values obtained by other methods. Hexane formation is of some importance at low light intensities and high ethylene concentrations, and is adequately accounted for by the reactions[Formula: see text]


F1000Research ◽  
2013 ◽  
Vol 2 ◽  
pp. 142
Author(s):  
Phillip B Grovenstein ◽  
Darryel A Wilson ◽  
Kathryn D Lankford ◽  
Kelsey A Gaston ◽  
Surangi Perera ◽  
...  

The green micro-alga Chlamydomonas reinhardtii is an elegant model organism to study all aspects of oxygenic photosynthesis. Chlorophyll (Chl) and heme are major tetrapyrroles that play an essential role in energy metabolism in photosynthetic organisms. These tetrapyrroles are synthesized via a common branched pathway that involves mainly nuclear encoded enzymes. One of the enzymes in the pathway is Mg chelatase (MgChel) which inserts Mg2+ into protoporphyrin IX (PPIX, proto) to form Magnesium-protoporphyrin IX (MgPPIX, Mgproto), the first biosynthetic intermediate in the Chl branch. The GUN4 (genomes uncoupled 4) protein is not essential for the MgChel activity but has been shown to significantly stimulate its activity. We have isolated a light sensitive mutant, 6F14, by random DNA insertional mutagenesis. 6F14 cannot tolerate light intensities higher than 90-100 μmol photons m-2 s-1. It shows a light intensity dependent progressive photo-bleaching. 6F14 is incapable of photo-autotrophic growth under light intensity higher than 100 μmol photons m-2 s-1. PCR based analyses show that in 6F14 the insertion of the plasmid outside the GUN4 locus has resulted in a genetic rearrangement of the GUN4 gene and possible deletions in the genomic region flanking the GUN4 gene. Our gun4 mutant has a Chl content very similar to that in the wild type in the dark and is very sensitive to fluctuations in the light intensity in the environment unlike the earlier identified Chlamydomonas gun4 mutant. Complementation with a functional copy of the GUN4 gene restored light tolerance, Chl biosynthesis and photo-autotrophic growth under high light intensities in 6F14. 6F14 is the second gun4 mutant to be identified in C. reinhardtii. Additionally, we show that our two gun4 complements over-express the GUN4 protein and show a higher Chl content per cell compared to that in the wild type strain.


2017 ◽  
pp. 21 ◽  
Author(s):  
Javier Carmona-Jiménez ◽  
Gustavo Montejano-Zurita

The results of a phycofloristic study on three springs in the Huasteca Potosina region are presented; 67 species are reported. Floristic similarities and differences among the springsare analized, as well as the relationship between species and environmental factors. Microhabitas were defined in the springs as well as the principal algal asociations that characterize them. Light intensity and current velocity are the most important factors affecting the predominant growth forms present in this type of habitat.


PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8001 ◽  
Author(s):  
Jiangnan Sun ◽  
Xiaomei Chi ◽  
Mingfang Yang ◽  
Jingyun Ding ◽  
Dongtao Shi ◽  
...  

Small sea urchins Strongylocentrotus intermedius (1–2 cm of test diameter) are exposed to different environments of light intensities after being reseeded to the sea bottom. With little information available about the behavioral responses of S. intermedius to different light intensities in the environment, we carried out an investigation on how S. intermedius is affected by three light intensity environments in terms of phototaxis, foraging and righting behaviors. They were no light (zero lx), low light intensity (24–209 lx) and high light intensity (252–2,280 lx). Light intensity had obvious different effects on phototaxis. In low light intensity, sea urchins moved more and spent significantly more time at the higher intensity (69–209 lx) (P = 0.046). S. intermedius in high light intensity, in contrast, spent significantly more time at lower intensity (252–690 lx) (P = 0.005). Unexpectedly, no significant difference of movement (average velocity and total distance covered) was found among the three light intensities (P > 0.05). Foraging behavior of S. intermedius was significantly different among the light intensities. In the no light environment, only three of ten S. intermedius found food within 7 min. In low light intensity, nine of 10 sea urchins showed successful foraging behavior to the food placed at 209 lx, which was significantly higher than the ratio of the number (two of 10) when food was placed at 24 lx (P = 0.005). In the high light intensity, in contrast, significantly less sea urchins (three of 10) found food placed at the higher light intensity (2,280 lx) compared with the lower light intensity (252 lx) (10/10, P = 0.003). Furthermore, S. intermedius showed significantly longer righting response time in the high light intensity compared with both no light (P = 0.001) and low light intensity (P = 0.031). No significant difference was found in righting behavior between no light and low light intensity (P = 0.892). The present study indicates that light intensity significantly affects phototaxis, foraging and righting behaviors of S. intermedius and that ~200 lx might be the appropriate light intensity for reseeding small S. intermedius.


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