The Effect of Viewing Angle on the Appearance of Trilaminar Structures of Desmosomes

1976 ◽  
Vol 34 ◽  
pp. 220-221
Author(s):  
Richard J. Neal ◽  
Joy L. Paulson
Keyword(s):  
Human Skin ◽  
Serial Section ◽  
Viewing Angle ◽  
Serial Sections ◽  
Serial Sectioning ◽  
Adjacent Section ◽  
Size And Shape ◽  
Spinous Layer

Examination of desmosomes in the spinous region of the epidermis of human skin reveals that the trilaminar structure, usually associated with desmosomes, is not always observed. Desmosomes were examined utilizing serial sectioning and tilting techniques to determine if the trilaminar structure is consistant throughout the desmosome. Concomitantly, information concerning the size and shape of the spinous layer desmosomes was obtained from examination of serial sections that completely transversed many desmosomes.Figures 1 and 2 are from a serial section series and demonstrate that the trilaminar structure of desmosomes (Dl and D2) can be observed in one section (figure 1) and not observed in an adjacent section (figure 2). Figure 3 thru Figure 6 are micrographs of the same section seen in Figure 2. These micrographs show the effect on the appearance of desmosomes Dl and D2 when they have been tilted about their longitudinal axes 5, 10, 20, and 30 degrees, respectively.

scholarly journals Form of the postsynaptic density. A serial section study.

1978 ◽  
Vol 78 (1) ◽  
pp. 36-46 ◽  
Author(s):  
R S Cohen ◽  
P Siekevitz
Keyword(s):  
Cerebral Cortex ◽  
Serial Section ◽  
Synaptic Vesicles ◽  
Synaptic Cleft ◽  
The Other ◽  
Serial Sections ◽  
Serial Sectioning ◽  
Presynaptic Membrane ◽  
Synaptic Junction ◽  
Cerebral Cortex Tissue

Through the use of serial sectioning of dog cerebral cortex tissue, holes or perforations could be revealed in the larger postsynaptic densities (PSDs), in confirmation of the earlier work of Peters and Kaisermann-Abramof (1969. Z. Zellforsch. Mikrosk. Anat. 100:487-506). These holes appeared in serial sections which happened to be cut both parallel and normal to the plane of the synaptic junction. Cleft material was absent in that part of the synaptic cleft opposite this hole. Somestimes the presynaptic membrane opposite the hole was indented into the presynaptic cell. In addition, most of the synaptic vesicles in the presynaptic cell close to the membrane were clustered at that part of the membrane opposite the edge of the density disk. The meaning of the hole and of the other features mentioned above for the function of the density is not known at present.

Cell morphology, volume, and surface area determinations from multilevel contouring within HVEM micrographs of serial sections and whole-cell mounts

1987 ◽  
Vol 45 ◽  
pp. 588-589
Author(s):  
M. Marko ◽  
A. Leith ◽  
D. Parsons
Keyword(s):  
Surface Area ◽  
Electron Micrographs ◽  
Cell Morphology ◽  
Individual Variation ◽  
Serial Section ◽  
Stereo Pair ◽  
Complex Structures ◽  
Serial Sections ◽  
Surface Areas ◽  
Computer Based

The use of serial sections and computer-based 3-D reconstruction techniques affords an opportunity not only to visualize the shape and distribution of the structures being studied, but also to determine their volumes and surface areas. Up until now, this has been done using serial ultrathin sections.The serial-section approach differs from the stereo logical methods of Weibel in that it is based on the Information from a set of single, complete cells (or organelles) rather than on a random 2-dimensional sampling of a population of cells. Because of this, it can more easily provide absolute values of volume and surface area, especially for highly-complex structures. It also allows study of individual variation among the cells, and study of structures which occur only infrequently.We have developed a system for 3-D reconstruction of objects from stereo-pair electron micrographs of thick specimens.

scholarly journals EXTIRPATION OF THE THYMUS IN THE GUINEA PIG

1917 ◽  
Vol 25 (1) ◽  
pp. 129-152 ◽  
Author(s):  
Edwards A. Park
Keyword(s):  
Guinea Pig ◽  
Serial Section ◽  
Guinea Pigs ◽  
Close Association ◽  
Main Lobe ◽  
Technical Error ◽  
Serial Sections ◽  
Pharyngeal Pouch ◽  
The Third ◽  
Third Pharyngeal Pouch

1. Accessory lobes of thymus, derived from the third pharyngeal pouch, occurring in close association with the parathyroids from the third pouch, were found in serial section of the cervical tissues of eleven out of fourteen guinea pigs, and probably would have been found in all fourteen but for a technical error. 2. It is probable, therefore, that accessory lobes of thymus having this situation and origin are usually, if not always, present in the guinea pig. 3. Additional accessory lobes of thymus belonging to, but at some distance from the main lobe were also present in several of the animals. 4. The discovery of these accessory lobes makes it certain that the guinea pig is unsuitable material for complete thymectomy, and probably complete extirpation of the thymus in this animal is rarely, if ever accomplished. 5. The extirpation experiments of previous investigators in the guinea pig must now be regarded as partial extirpations, and their results interpreted in that light. 6. Extirpation of the thymus in the guinea pig produced no changes in the writer's experiments. 7. The study of the serial sections of the cervical tissues of the guinea pig indicates that Ruben's statements regarding the parathyroid derived from the fourth pharyngeal pouch in the guinea pig are correct,—that it is much smaller than parathyroid III, may be rudimentary, and is sometimes absent at least on one side. 8. No accessory lobe of thymus was found accompanying the parathyroid from the fourth pouch, a finding also bearing out Ruben's statement that no thymus anlarge springs from the fourth pouch in the guinea pig.

scholarly journals Life Sentence: Mollusc Prisoner in Cretaceous Brachiopod Reconstructed in 3D. First Evidence for the Co-Existence of A Mollusc Inside a Living Animal

2021 ◽  
Author(s):  
László Bujtor ◽  
Philippe Andrey
Keyword(s):  
Lower Cretaceous ◽  
Fossil Record ◽  
Lateral Wall ◽  
General Purpose ◽  
3D Modelling ◽  
Wide Field ◽  
Living Animal ◽  
Serial Sections ◽  
Serial Sectioning ◽  
Life Sentence

Abstract A tiny shell (7 mm length) of a juvenile Mollusc (supposed gastropod) was discovered enclosed within the packstone filled shell of a terebratellidine brachiopod (Zittelina hofmanni Bujtor and Vörös, 2020) from the Lower Cretaceous Apátvarasd Limestone Formation of Zengővárkony (Mecsek Mountains, South Hungary). Serial sectioning of the brachiopod shell revealed that in addition to the brachidium, a different and unusual shelly structure was preserved inside the brachiopod shell. 2D serial sections were digitalized and fed into Free-D imaging software to generate a 3D reconstruction of the entombed fossil, which is supposed a juvenile gastropod. Consideration of the emplacement, dimensions, and orientation of the shell suggests that it entered the cavity of the living brachiopod shell as an egg or larva and was trapped. Further analysis of both fossils suggests that the two animals lived and developed beside each other for a longer period: the brachiopod crus is less developed and slightly deformed on the gastropod side with the gastropod placed parallel to the crura and the lateral wall of the brachiopod. This example suggests that shells of brachiopods may have provided sheltered microhabitats for benthic micro- and macrofossils not only after the death but even during the life of the host brachiopod. This is the first evidence from the fossil record for the co-existence of a living Mollusc inside the shell of a living brachiopod. This work illustrates how general purpose 3D modelling software initially developed in other disciplines may provide new insights in the wide field of geosciences.

Preparation of serial sections

1989 ◽  
Vol 4 ◽  
pp. 146-156 ◽  
Author(s):  
Michael R. Sandy
Keyword(s):  
Three Dimensional ◽  
Diamond Wheel ◽  
Three Dimensions ◽  
Serial Sections ◽  
Serial Sectioning ◽  
Permanent Record ◽  
Glass Slides ◽  
Internal Structures ◽  
Abrasive Surface ◽  
Saw Blade

Serial sectioning (also referred to as serial grinding) is used to investigate the internal structures of three-dimensional (rock or fossil). In this process series of sections are ground or cut in sequence through a specimen to reveal its internal structures. The specimen is ground down against an abrasive surface (e.g., abrasive powder on a sheet of steel or a rotating diamond wheel on a lathe) or cut with a saw blade. The details of each section can be recorded by drawing or photography. A permanent record of each surface can be made by taking acetate peels and mounting them in glass slides (Wilson and Palmer, this volume, Chapter 13). Serial section information can be digitized and reconstructed in three-dimensions using computer techniques (Chapman, this volume, Chapter 15).

Serial sections and stereoscopy-complementary approaches to three-dimensional reconstruction

1986 ◽  
Vol 44 ◽  
pp. 36-37 ◽  
Author(s):  
John C. Russ ◽  
Thomas M. Hare
Keyword(s):  
Surface Area ◽  
Serial Section ◽  
Three Dimensional ◽  
Color Images ◽  
Physical Models ◽  
Serial Sections ◽  
Transparent Material ◽  
Modern Computer ◽  
Computer Equipment ◽  
Dimensional Reconstruction

Modelling of three-dimensional structures, either for purposes of geometrical measurement (eg. volume, surface area) or as an aid to visualization, has traditionally been carried out by a variety of different methods. Biologists, who are usually able to conveniently cut sections through their specimens, often make use of serial sections for this purpose. The most common interpretation of serial section photos has been by printing micrographs on transparent material, aligning them, and stacking them up. Occasionally, physical models of lucite, wood, clay or styrofoam have been constructed using the prints as templates, and with the advent of modern computer equipment, some digitization of the sections and their subsequent viewing or plotting with any viewpoint and orientation has enabled researchers to better see the structures represented. There has even been limited use of stereoscopy, that is, producing plots or on-screen color images of the feature outlines from two different viewpoints which can be visually merged to produce the illusion of relief.

Serial Sectioning of Refractory Locust Eggs

1964 ◽  
Vol s3-105 (71) ◽  
pp. 379-380
Author(s):  
D. S. ANDERSON
Keyword(s):  
Formic Acid ◽  
Picric Acid ◽  
Serial Sections ◽  
Serial Sectioning

A technique is described for sectioning the yolky tissue of locust eggs. It is a modified version of a method evolved by Griffiths and Carter and successfully used on reptile eggs. The fixative is dioxan saturated with picric acid, with 5% formic acid and 10% formaldehyde. It rapidly penetrates the yolk and minimizes hardening. Dehydration in cellosolve is followed by clearing in benzene. After embedding in wax the block is immersed in 5% tergitol ethane-diol solution, and 12 h later good serial sections are obtained.

Characterization of Conductive Anodic Filament (Caf) by X-Ray Microtomography and by Serial Sectioning

1993 ◽  
Vol 323 ◽  
Author(s):  
S. R. Stock ◽  
L. L. Dollar ◽  
G. B. Freeman ◽  
W. J. Ready ◽  
L. J. Turbini ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
Serial Sections ◽  
Printed Wiring Boards ◽  
Serial Sectioning ◽  
X Ray ◽  
Backscattered Electrons ◽  
Conductive Anodic Filament

ABSTRACTX-ray microtomography is used to nondestructively section printed wiring boards in which conductive anodic filaments (CAFs) had grown, Quantification of the spatial distribution of copper is compared for microtomography and for serial sections obtained in SEM with backscattered electrons. The agreement between the techniques is excellent and indicates that microtomography may be used confidently to follow the subsurface growth of CAFs.

An Electron Backscattered Diffraction Specimen Preparation Technique for High Resolution Serial Sectioning

2000 ◽  
Vol 6 (S2) ◽  
pp. 950-951
Author(s):  
M.A. Wall ◽  
A.J. Schwartz ◽  
L. Nguyen
Keyword(s):  
High Resolution ◽  
Serial Section ◽  
Orientation Imaging Microscopy ◽  
Side Surface ◽  
Primary Objective ◽  
Measuring System ◽  
Specimen Preparation ◽  
Preparation Technique ◽  
Serial Sectioning ◽  
Electron Backscattered Diffraction

A high-resolution serial sectioning specimen preparation technique is described for acquisition of electron backscattered diffraction orientation imaging microscopy (OIM) data. The primary objective is to develop a method to reproducibly remove a controlled thickness of material (a serial section) from a polycrystalline Ta sample while producing quality surfaces for OIM imaging. This is integrated with the ability to accurately measure the amount of material removed with each iteration and experimentally register the ensuing OIM scans.A Si metrology device is fabricated by lithographic techniques to facilitate the measurement of the amount of material removed with each lapping iteration and the alignment of each serial section image. This metrology device contains a herringbone pattern with two orthogonal etched lines oriented at 45 degrees and one vertical line normal to the OIM surface. The metrology device is epoxied to a polished side surface of the Ta sample. Due to the orthogonality of the lines, the thickness of material removed equals one-half the total linear motion of the etched lines as seen in cross-section. To accurately measure the distance between the grooves after each lapping iteration, a high-resolution (0.1 μm) linear measuring system is attached to the table of a light metallograph.

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