Time-lapse x ray microscope movie of the germinating garden pea seed
The plant embryo is relatively insensitive to x-rays. One should like to take advantage of this property to follow the early processes of seed germination by time-lapse x-ray microscopy. Preliminary work has shown that radiation exposure can be reduced by two orders of magnitude if a minimum number of frames are exposed and the movie then generated by slowly dissolving one frame into the next. Image processing also helps the problem of low contrast in the living image. Present work has been directed against the problem that geotropism makes the embryo grow out of the plane of the picture (the instrument does not operate horizontally). The approach taken should also simplify the problems of maintaining a suitable environment for the seed and make the instrument available between exposures when working with seeds which have long germination times. The garden pea was chosen as a test material because of its environmental tolerance, short maturation cycle, and similarity to pine seeds in size and radiation tolerance.