Intracellular interactions of Leishmania mexicana with a cell line from Toxorhynchites amboinensis (77M-248) larvae

1994 ◽  
Vol 52 ◽  
pp. 268-269
Author(s):  
Carlos Argüello
Keyword(s):  
Cell Line ◽  
Membrane Receptors ◽  
Specific Cell ◽  
Leishmania Mexicana ◽  
Amastigote Form ◽  
Different Temperatures ◽  
Two Distinct Temperatures ◽  
A Cell ◽  
Promastigote Form

Leishmania is an intracellular parasite that lives in phagocytic vacuoles of macrophages . The promastigote form of the parasite is flagellated and interacts with macrophages through specific cell membrane receptors . Once inside phagocytic vacuoles, the parasite differentiates into the amastigote form which is small and aflagellated. Part of the life cycle of the parasite occurs in the lumen of the digestive tract of an insect, with a body temperature of 25-27 °C that promotes the preferential differentiation of the promastigote form. A cell line ( TRA-248 ) isolated from Toxorhynchites amboinensis has been adapted to grow at two different temperatures 24° and 34°C, and used in the study of dengue virus. This quality of TRA-248 cells is of relevance since they permit to analyze the behavior of leishmania in an insect cell at two distinct temperatures. In this study, TRA- 248 cells and promastigotes interacted in culture at 24° and 34°C, for 12, 24 and 48 hrs and determine if the parasites are phagocyted and able to differentiate into the amastigote form. Also, the activity of acid phosphatase was compared between TRA-248 cells and macrophages.

scholarly journals The effects of Con A on cell surface shedding in cell cultures

1980 ◽  
Vol 46 (1) ◽  
pp. 221-234
Author(s):  
T.C. Doetschman
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Muscle Cell ◽  
Cell Cultures ◽  
Fibroblast Cell ◽  
Cell Types ◽  
Mouse Fibroblast ◽  
Specific Cell ◽  
Con A ◽  
A Cell

A cell surface immobilizing concentration of Con A inhibits the shedding of [3H]fucose-containing glycoproteins from the surface of chick embryonic leg and breast muscle cell cultures and cultures of the rat skeletal muscle cell line L6. The Con A-induced inhibition of shedding is much less in the mouse fibroblast cell line 3T3. In all 4 cell types the lectin inhibits the shedding of some fucosyl-glycoproteins more than others, especially those of a lipid-containing fraction which is excluded in Biogel A-5m chromatography. This differential nature of the Con A effect is not changed by the cytoskeletal disruptors cytochalasin B or colchicine. Con A causes an increase in the amount of trypsin-sensitive surface fucosylglycoprotein in the cell surface and appears to decrease the overall amount of cell surface degradation suggesting the inhibition of shedding caused by Con A is not due to an increase in internalization and degradation. The data suggest that some shedding may occur at specific cell surface sites to which surface materials must laterally migrate.

scholarly journals A new epithelial cell line, SSK from kidney of striped snakehead Channa striatus (Bloch 1793)

2017 ◽  
Vol 64 ◽  
Author(s):  
D. K. Chaudhary ◽  
N. Sood ◽  
D. K. Verma ◽  
T. R. Swaminathan ◽  
B. Kushwaha ◽  
...  
Keyword(s):  
Cell Line ◽  
Neutral Red ◽  
Epithelial Cell Line ◽  
Channa Striatus ◽  
Neutral Red Assay ◽  
Mammalian Expression ◽  
Cytotoxicity Studies ◽  
Different Temperatures ◽  
A Cell ◽  
Foetal Bovine Serum

A cell line was established from striped snakehead Channa striatus kidney. The cell line, named as SSK, has been passaged for over 62 times. Growth studies at different temperatures and foetal bovine serum (FBS) concentrations revealed that SSK cells show optimum growth at 28°C in L-15 medium containing 20% FBS. The chromosome number of SSK cells was 2n=40. Partial sequencing of mitochondrial cytochrome oxidase 1 gene of the cells confirmed that the cell line originated from C. striatus. The SSK cells were primarily epithelial, as determined using immunophenotyping. The cells from SSK cell line were transfected with phrGFP II-N mammalian expression vector. The SSK cell line was stored in liquid nitrogen (-196οC) at different passages and was successfully revived after four months of storage. The cell line could be successfullyemployed for cytotoxicity studies, as revealed by neutral red assay. The cell line can be a valuable surrogate to the whole fish studies.

Proteinases of Leishmania mexicana and other flagellate protozoa

Parasitology ◽  
1982 ◽  
Vol 84 (1) ◽  
pp. 149-155 ◽  
Author(s):  
G. H. Coombs
Keyword(s):  
Primary Factor ◽  
Proteinase Activity ◽  
Cysteine Proteinases ◽  
Human Pathogen ◽  
Leishmania Mexicana ◽  
Amastigote Form ◽  
Promastigote Form ◽  
Survival And Growth ◽  
Very High

SUMMARYThe amastigote form of the human pathogen Leishmania mexicana contains high proteinase activity, some 20 times greater than that in the promastigote form and macrophages and appreciably higher than the activity in other flagellate protozoa. The main amastigote enzymes are soluble, whereas those of the promastigote are particulate, and have inhibitor sensitivities characteristic of cysteine proteinases. The very high soluble proteinase activity of L. mexicana amastigotes may be a primary factor in the survival and growth of this mammalian stage in its potentially degradative intracellular habitat.

Aberrant Type C Virus Production in a Cell Line Derived from Balb/3T3

1972 ◽  
Vol 30 ◽  
pp. 286-287
Author(s):  
N. Savage ◽  
A. Hackett
Keyword(s):  
Electron Microscopy ◽  
Cell Line ◽  
Leukemia Virus ◽  
Morphological Characteristics ◽  
Virus Production ◽  
Oncogenic Viruses ◽  
Endogenous Virus ◽  
Virus Particles ◽  
Moloney Leukemia Virus ◽  
A Cell

A cell line, UC1-B, which was derived from Balb/3T3 cells, maintains the same morphological characteristics of the non-transformed parental culture, and shows no evidence of spontaneous virus production. Survey by electron microscopy shows that the cell line consists of spindle-shaped cells with no unusual features and no endogenous virus particles.UC1-B cells respond to Moloney leukemia virus (MLV) infection by a change in morphology and growth pattern which is typical of cells transformed by sarcoma virus. Electron microscopy shows that the cells are now variable in shape (rounded, rhomboid, and spindle), and each cell type has some microvilli. Virtually all (90%) of the cells show virus particles developing at the cell surface and within the cytoplasm. Maturing viruses, typical of the oncogenic viruses, are found along with atypical tubular forms in the same cell.

Establishment of a cell line (HCC-M) from a human hepatocellular carcinoma

1983 ◽  
Vol 32 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Tetsu Watanabe ◽  
Toshio Morizane ◽  
Kanji Tsuchimoto ◽  
Yasutaka Inagaki ◽  
Yoshio Munakata ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Human Hepatocellular Carcinoma ◽  
A Cell

scholarly journals N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis

Antioxidants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 860
Author(s):  
Wu-Sheng Sun ◽  
Hoon Jang ◽  
Mi-Ryung Park ◽  
Keon Bong Oh ◽  
Haesun Lee ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Early Stage ◽  
Developmental Competence ◽  
Beneficial Effects ◽  
Developmental Rates ◽  
A Cell ◽  
Bovine Oocytes ◽  
Dose Dependent

Oxidative stress has been suggested to negatively affect oocyte and embryo quality and developmental competence, resulting in failure to reach full term. In this study, we investigated the effect of N-acetyl-L-cysteine (NAC), a cell-permeating antioxidant, on developmental competence and the quality of oocytes and embryos upon supplementation (0.1–10 mM) in maturation and culture medium in vitro using slaughterhouse-derived oocytes and embryos. The results show that treating oocytes with 1.0 mM NAC for 8 h during in vitro maturation attenuated the intracellular reactive oxygen species (ROS) (p < 0.05) and upregulated intracellular glutathione levels (p < 0.01) in oocytes. Interestingly, we found that NAC affects early embryonic development, not only in a dose-dependent, but also in a stage-specific, manner. Significantly (p < 0.05) decreased cleavage rates (90.25% vs. 81.46%) were observed during the early stage (days 0–2), while significantly (p < 0.05) increased developmental rates (38.20% vs. 44.46%) were observed during the later stage (from day 3) of embryonic development. In particular, NAC supplementation decreased the proportion of apoptotic blastomeres significantly (p < 0.05), resulting in enhanced hatching capability and developmental rates during the in vitro culture of embryos. Taken together, our results suggest that NAC supplementation has beneficial effects on bovine oocytes and embryos through the prevention of apoptosis and the elimination of oxygen free radicals during maturation and culture in vitro.

Proteinase and phospholipase activities and development at different temperatures of yeasts isolated from bovine milk

2011 ◽  
Vol 78 (4) ◽  
pp. 385-390 ◽  
Author(s):  
Priscilla A Melville ◽  
Nilson R Benites ◽  
Monica Ruz-Peres ◽  
Eugenio Yokoya
Keyword(s):  
Dairy Products ◽  
Room Temperature ◽  
Bovine Milk ◽  
Raw Milk ◽  
Pathogenic Potential ◽  
Proteinase Production ◽  
Different Temperatures ◽  
Quality Of Milk ◽  
Means Of Transmission

The presence of yeasts in milk may cause physical and chemical changes limiting the durability and compromising the quality of the product. Moreover, milk and dairy products contaminated by yeasts may be a potential means of transmission of these microorganisms to man and animals causing several kinds of infections. This study aimed to determine whether different species of yeasts isolated from bovine raw milk had the ability to develop at 37°C and/or under refrigeration temperature. Proteinase and phospholipase activities resulting from these yeasts were also monitored at different temperatures. Five genera of yeasts (Aureobasidium sp., Candida spp., Geotrichum spp., Trichosporon spp. and Rhodotorula spp.) isolated from bovine raw milk samples were evaluated. All strains showed one or a combination of characteristics: growth at 37°C (99·09% of the strains), psychrotrophic behaviour (50·9%), proteinase production (16·81% of the strains at 37°C and 4·09% under refrigeration) and phospholipase production (36·36% of the isolates at 37°C and 10·9% under refrigeration), and all these factors may compromise the quality of the product. Proteinase production was similar for strains incubated at 37°C (16·81% of the isolates) and room temperature (17·27%) but there was less amount of phospholipase-producing strains at room temperature (15·45% of the isolates were positive) when compared with incubation at 37°C (36·36%). Enzymes production at 37°C by yeasts isolated from milk confirmed their pathogenic potential. The refrigeration temperature was found to be most efficient to inhibit enzymes production and consequently ensure better quality of milk. The viability of yeasts and the activity of their enzymes at different temperatures are worrying because this can compromise the quality of dairy products at all stages of production and/or storage, and represent a risk to the consumer.

scholarly journals Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics

Blood ◽  
1985 ◽  
Vol 65 (1) ◽  
pp. 21-31 ◽  
Author(s):  
RC Stong ◽  
SJ Korsmeyer ◽  
JL Parkin ◽  
DC Arthur ◽  
JH Kersey
Keyword(s):  
Acute Leukemia ◽  
Cell Line ◽  
Lymphoblastic Leukemia ◽  
Leukemia Cell ◽  
Leukemic Cells ◽  
Leukemia Cell Line ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Immunoglobulin Gene ◽  
A Cell ◽  
B Lineage

Abstract A cell line, designated RS4;11, was established from the bone marrow of a patient in relapse with an acute leukemia that was characterized by the t(4;11) chromosomal abnormality. The cell line and the patient's fresh leukemic cells both had the t(4;11)(q21;q23) and an isochromosome for the long arm of No. 7. Morphologically, all cells were lymphoid in appearance. Ultrastructurally and cytochemically, approximately 30% of the cells possessed myeloid features. The cells were strongly positive for terminal deoxynucleotidyl transferase. They were HLA-DR positive and expressed surface antigens characteristic for B lineage cells, including those detected by anti-B4, BA-1, BA-2, and PI153/3. Immunoglobulin gene analysis revealed rearrangements of the heavy chain and kappa chain genes. The cells lacked the common acute lymphoblastic leukemia antigen and antigenic markers characteristic of T lineage cells. The cells reacted with the myeloid antibody 1G10 but not with other myeloid monoclonal antibodies. Treatment with 12-O-tetradecanoyl- phorbol-13-acetate induced a monocyte-like phenotype demonstrated by cytochemical, functional, immunologic, and electron microscopic studies. The expression of markers of both early lymphoid and early myeloid cells represents an unusual phenotype and suggests that RS4;11 represents a cell with dual lineage capabilities. To our knowledge, RS4;11 is the first cell line established from t(4;11)-associated acute leukemia.

scholarly journals Divergent Pathways Regulate Ligand-Independent Activation of ERα in SK-N-BE Neuroblastoma and COS-1 Renal Carcinoma Cells

1998 ◽  
Vol 12 (6) ◽  
pp. 835-841
Author(s):  
Cesare Patrone ◽  
Elisabetta Gianazza ◽  
Sabrina Santagati ◽  
Paola Agrati ◽  
Adriana Maggi
Keyword(s):  
Cell Line ◽  
Intracellular Signaling ◽  
Steroid Receptors ◽  
Neuroblastoma Cell ◽  
Cross Coupling ◽  
Activation Function ◽  
Neuroblastoma Cell Line ◽  
Membrane Receptors ◽  
Insulin Dependent ◽  
The Cross

Abstract The α-estrogen receptor (ERα) transcriptional activity can be regulated either by binding to the cognate ligand or by intracellular signaling pathways responsive to a variety of factors acting through cell membrane receptors. Studies carried out in HeLa and COS-1 cells demonstrated that the cross-coupling between estrogen and growth factor receptors is mediated by p21ras and requires phosphorylation of a specific serine residue (Ser 118 in the human ERα and Ser 122 in mouse ERα) located in the ERα N-terminal activation function 1 (AF-1). Likewise, in the SK-N-BE neuroblastoma cell line p21ras is involved in the cross-coupling between insulin and ERα receptors. However, in this cell line Ser 122 is not necessary for insulin-dependent activation of unliganded ERα. In addition, after insulin activation, the electrophoretic mobility associated to serine hyperphosphorylation of ERα in SK-N-BE and in COS-1 cells is different. Our study rules out the possibility of tyrosine phosporylation in unliganded ERα activation by means of transactivation studies of ERα tyrosine mutants and analysis of Tyr phosphorylation immunoreactivity. The two cofactors for steroid receptors RIP 140 and SRC-1 do not seem to be specifically involved in the insulin-induced ERα transactivation. The present study demonstrates the possibility of an alternative, cell-specific pathway of cross-coupling between intracellular and membrane receptors, which might be of importance for the understanding of the physiological significance of this mode of activation in the nervous system.

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