Bartonella vinsonii subsp. berkhoffii andB.henselaein dogs

AbstractThis study aimed to molecularly surveyBartonellain dogs from Chile. Quantitative real-time PCR (qPCR) forBartonellaspp. based onnuoGgene was performed in 139 blood samples taken from dogs belonging to rural localities of the Valdivia Province, Los Ríos region, southern Chile.nuoGqPCR-positive samples were submitted to conventional PCR assays forftsZ,gltA,rpoBandnuoGgenes and sequencing for speciation and phylogenetic analysis. Based upon qPCR results,Bartonellaspp. occurrence in dogs was 4.3% (6/139). Out of sixnuoGqPCR-positive samples, six, three, two and none showed positive results in cPCR assays based ongltA,ftsZ,rpoBandnuoGgenes, respectively. Consistent sequencing results were obtained only for theftsZgene from sample #1532 (GeneBank accession number: MG252491), andgltAgene from samples #1535 (MG252490) and #1532 (148 bp fragment that was not deposited in GenBank). Phylogenetic analysis offtsZandgltAgenes allowed speciation of twonuoG-positive samples, one asBartonella vinsoniisubsp. berkhoffii and the other asB. henselae. Bartonella vinsoniisubsp. berkhoffii andB. henselaeare detected for the first time in dogs from Chile, highlighting the importance of the canine population as a source of zoonotic agents and potential infection risk to humans.

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Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-296120180069 ◽

2018 ◽

Vol 27 (4) ◽

pp. 505-513 ◽

Cited By ~ 2

Author(s):

Anna Cláudia Baumel Mongruel ◽

Priscila Ikeda ◽

Keyla Carstens Marques de Sousa ◽

Jyan Lucas Benevenute ◽

Margarete Kimie Falbo ◽

...

Keyword(s):

18S Rrna ◽

Molecular Detection ◽

Phylogenetic Analyses ◽

Southern Brazil ◽

Conventional Pcr ◽

Pathogenic Potential ◽

Vector Borne ◽

Pcr Assays ◽

Etiological Agents ◽

Positive Results

Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli.

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Molecular Autograph of Maturase-K Gene in Isodon rugosus (Lamiaceae)

Botanica ◽

10.2478/botlit-2020-0009 ◽

2020 ◽

Vol 26 (1) ◽

pp. 95-100

Author(s):

Devendra Kumar Srivastava ◽

Pekhna Bansal ◽

Pradeep Kumar Singh ◽

Manjit Inder Singh Saggoo

Keyword(s):

Phylogenetic Analysis ◽

Maximum Likelihood ◽

Medicinal Plants ◽

Dna Sequence ◽

Accession Number ◽

Candidate Marker ◽

Isodon Rugosus ◽

Dna Sequence Alignment ◽

Species Specific ◽

First Time

AbstractMolecular autograph of trn/mat-K gene was used as one of the important candidate marker in addressing the questions of systematics and barcoding of medicinal plants. Features of trn/mat-K gene in Isodon rugosus (Lamiaceae) were assessed for the first time (NCBI GenBank Accession Number: MH939199.1). Sequence of 756 bp length was amplified by the universal matK primers (matK472F and matK1248R) in the cpDNA of the plant. It was reported to contain trnK gene (>1…..50; >746…..756), partial sequence; and matK gene (>51…..745), partial coding DNA sequence. Alignment search and analysis showed that only nine different Isodon species are currently available for matK sequences with repeated copy of submissions in GenBank. The matK sequences of I. rugosus was reported with 34.2 (G + C)% and 17 variable sites (VS), out of which seven were singleton (ST) and 10 sites were species-specific parsimoniously-informative (PI) that could be used to differentiate I. rugosus from other species as well as to authenticate the taxon. Phylogenetic analysis resulted into monophyletic clustering of I. rugosus near to the clade having I. coetsa by both maximum likelihood (ML) and maximum parsimony (MP) methods. Clades obtained in ML tree were more informative as compared to MP tree.

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Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

Viruses ◽

10.3390/v10090486 ◽

2018 ◽

Vol 10 (9) ◽

pp. 486 ◽

Cited By ~ 1

Author(s):

Christina Lazov ◽

Mariann Chriél ◽

Hans Baagøe ◽

Esben Fjederholt ◽

Yu Deng ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Rt Pcr ◽

The United Kingdom ◽

Faecal Samples ◽

Myotis Daubentonii ◽

Pcr Assays ◽

Species Specific ◽

First Time ◽

Geographical Locations

Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

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New and noteworthy lichen-forming and lichenicolous fungi 10

Acta Botanica Hungarica ◽

10.1556/034.62.2020.1-2.6 ◽

2020 ◽

Vol 62 (1-2) ◽

pp. 69-108

Author(s):

S. Y. Kondratyuk ◽

D. K. Upreti ◽

G. K. Mishra ◽

S. Nayaka ◽

K. K. Ingle ◽

...

Keyword(s):

Phylogenetic Analysis ◽

South Korea ◽

Eastern Europe ◽

South Asia ◽

New Combinations ◽

Eastern Asia ◽

Forest Zone ◽

Mediterranean Europe ◽

First Time ◽

India And China

Eight species, new for science, i.e.: Lobothallia gangwondoana S. Y. Kondr., J.-J. Woo et J.-S. Hur and Phyllopsora dodongensis S. Y. Kondr. et J.-S. Hur from South Korea, Eastern Asia, Ioplaca rinodinoides S. Y. Kondr., K. K. Ingle, D. K. Upreti et S. Nayaka, Letrouitia assamana S. Y. Kondr., G. K. Mishra et D. K. Upreti, and Rusavskia indochinensis S. Y. Kondr., D. K. Upreti et S. Nayaka from India and China, South Asia, Caloplaca orloviana S. Y. Kondr. and Rusavskia drevlyanica S. Y. Kondr. et O. O. Orlov from Ukraine, Eastern Europe, as well as Xanthoria ibizaensis S. Y. Kondr. et A. S. Kondr. from Ibiza Island, Spain, Mediterranean Europe, are described, illustrated and compared with closely related taxa. Fominiella tenerifensis S. Y. Kondr., Kärnefelt, A. Thell et Feuerer is for the first time recorded from Mediterranean Europe, Huriella loekoesiana S. Y. Kondr. et Upreti is provided from Russia for the first time, and H. pohangensis S. Y. Kondr., L. Lőkös et J.-S. Hur for the first time from China, Phoma candelariellae Z. Kocakaya et Halıcı is new to Ukraine, and Staurothele frustulenta Vain. is recorded from the Forest Zone of Ukraine for the first time. Twelve new combinations, i.e.: Bryostigma apotheciorum (for Sphaeria apotheciorum A. Massal.), Bryostigma biatoricola (for Arthonia biatoricola Ihlen et Owe-Larss.), Bryostigma dokdoense (for Arthonia dokdoensis S. Y. Kondr., L. Lőkös, B. G. Lee, J.-J. Woo et J.-S. Hur), Bryostigma epiphyscium (for Arthonia epiphyscia Nyl.), Bryostigma lobariellae (for Arthonia lobariellae Etayo), Bryostigma lapidicola (for Lecidea lapidicola Taylor), Bryostigma molendoi (for Tichothecium molendoi Heufl. ex Arnold), Bryostigma neglectulum (for Arthonia neglectula Nyl.), Bryostigma parietinarium (for Arthonia parietinaria Hafellner et Fleischhacker), Bryostigma peltigerinum (for Arthonia vagans var. peltigerina Almq.), Bryostigma phaeophysciae (for Arthonia phaeophysciae Grube et Matzer), Bryostigma stereocaulinum (for Arthonia nephromiaria var. stereocaulina Ohlert), are proposed based on results of combined phylogenetic analysis based on mtSSU and RPB2 gene sequences. Thirty-one new combinations for members of the genus Polyozosia (i.e.: Polyozosia actophila (for Lecanora actophila Wedd.), Polyozosia agardhiana (for Lecanora agardhiana Ach.), Polyozosia altunica (for Myriolecis altunica R. Mamut et A. Abbas), Polyozosia antiqua (for Lecanora antiqua J. R. Laundon), Polyozosia bandolensis (for Lecanora bandolensis B. de Lesd.), Polyozosia behringii (for Lecanora behringii Nyl.), Polyozosia caesioalutacea (for Lecanora caesioalutacea H. Magn.), Polyozosia carlottiana (for Lecanora carlottiana C. J. Lewis et Śliwa), Polyozosia congesta (for Lecanora congesta Clauzade et Vězda), Polyozosia eurycarpa (for Lecanora eurycarpa Poelt, Leuckert et Cl. Roux), Polyozosia expectans (Lecanora expectans Darb.), Polyozosia flowersiana (Lecanora flowersiana H. Magn.), Polyozosia fugiens (for Lecanora fugiens Nyl.), Polyozosia invadens (for Lecanora invadens H. Magn.), Polyozosia juniperina (for Lecanora juniperina Śliwa), Polyozosia latzelii (for Lecanora latzelii Zahlbr.), Polyozosia liguriensis (for Lecanora liguriensis B. de Lesd.), Polyozosia massei (for Myriolecis massei M. Bertrand et J.-Y. Monnat), Polyozosia mons-nivis (for Lecanora mons-nivis Darb.), Polyozosia oyensis (for Lecanora oyensis M.-P. Bertrand et Cl. Roux), Polyozosia percrenata (for Lecanora percrenata H. Magn.), Polyozosia persimilis (for Lecanora hagenii subsp. persimilis Th. Fr.), Polyozosia poeltiana (for Lecanora poeltiana Clauzade et Cl. Roux), Polyozosia prominens (for Lecanora prominens Clauzade et Vězda), Polyozosia prophetae-eliae (for Lecanora prophetae-eliae Sipman), Polyozosia salina (for Lecanora salina H. Magn.), Polyozosia schofieldii (for Lecanora schofieldii Brodo), Polyozosia sverdrupiana (for Lecanora sverdrupiana Øvstedal), Polyozosia torrida (for Lecanora torrida Vain.), Polyozosia wetmorei (for Lecanora wetmorei Śliwa), Polyozosia zosterae (for Lecanora subfusca? zosterae Ach.)) are proposed.

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Pressurized Hot Liquid Extraction with 15% v/v Glycerol-Water as an Effective Environment-Friendly Process to Obtain Durvillaea Incurvata and Lessonia Spicata Phlorotannin Extracts with Antioxidant and Antihyperglycemic Potential

Antioxidants ◽

10.3390/antiox10071105 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1105

Author(s):

Fernanda Erpel ◽

María Salomé Mariotti-Celis ◽

Javier Parada ◽

Franco Pedreschi ◽

José Ricardo Pérez-Correa

Keyword(s):

Brown Seaweed ◽

Dry Weight ◽

Liquid Extraction ◽

Southern Chile ◽

Polyphenol Content ◽

Glycerol Water ◽

Environment Friendly ◽

Dry Extract ◽

First Time ◽

Anatomical Part

Brown seaweed phlorotannins have shown the potential to promote several health benefits. Durvillaea incurvata and Lessonia spicata—species that are widely distributed in central and southern Chile—were investigated to obtain phlorotannin extracts with antioxidant and antihyperglycemic potential. The use of an environmentally friendly and food-grade glycerol-based pressurized hot liquid extraction (PHLE) process (15% v/v glycerol water) was assessed for the first time to obtain phlorotannins. Multiple effects were analyzed, including the effect of the species, harvesting area (Las Cruces and Niebla), and anatomical part (holdfast, stipe, and frond) on the extracts’ polyphenol content (TPC), antioxidant capacity (AC), and carbohydrate-hydrolyzing enzyme—α-glucosidase and α-amylase—inhibitory activity. Contaminants, such as mannitol, heavy metals (As, Cd, Pb, Hg, and Sn), and 5-hydroxymethylfurfural (HMF), were also determined. The anatomical part used demonstrated a significant impact on the extracts’ TPC and AC, with holdfasts showing the highest values (TPC: 95 ± 24 mg phloroglucinol equivalents/g dry extract; DPPH: 400 ± 140 μmol Trolox equivalents/g dry extract; ORAC: 560 ± 130 μmol TE/g dry extract). Accordingly, holdfast extracts presented the most potent α-glucosidase inhibition, with D. incurvata from Niebla showing an activity equivalent to fifteen times that of acarbose. Only one frond and stipe extract showed significant α-glucosidase inhibitory capacity. No α-amylase inhibition was found in any extract. Although no HMF was detected, potentially hazardous cadmium levels (over the French limit) and substantial mannitol concentrations—reaching up to 50% of the extract dry weight—were found in most seaweed samples and extracts. Therefore, further purification steps are suggested if food or pharmaceutical applications are intended for the seaweed PHLE extracts obtained in this study.

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Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

AMB Express ◽

10.1186/s13568-020-01134-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Maroua Oueslati ◽

Magdalena Mulet ◽

Mohamed Zouaoui ◽

Charlotte Chandeysson ◽

Jorge Lalucat ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Pseudomonas Syringae ◽

Ice Nucleation ◽

Phylogenetic Study ◽

Future Studies ◽

Citrus Orchards ◽

First Time ◽

New Cultivars

Abstract The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.

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Detection and molecular characterization of enteric adenovirus in treated wastewater in the Brazilian Federal District

SN Applied Sciences ◽

10.1007/s42452-021-04678-2 ◽

2021 ◽

Vol 3 (7) ◽

Author(s):

T. S. C. Quintão ◽

F. G. Silva ◽

A. L. Pereira ◽

W. N. Araújo ◽

P. M. Oliveira ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Wastewater Treatment ◽

Wastewater Treatment Plants ◽

Federal District ◽

Treated Wastewater ◽

Conventional Pcr ◽

Rainfall Index ◽

Enteric Adenovirus ◽

Enteric Adenoviruses

AbstractHuman enteric viruses, such as enteric adenoviruses (HAdV), are known to be involved with gastrointestinal disorders, especially acute gastroenteritis. Several studies have used HAdV as an indicator of water quality, since they are considered highly stable and widely distributed viruses in water matrices. The aim of this study was to detect and genotype HAdVs in water matrices impacted by discharges of treated effluents from wastewater treatment plants (WWTPs). Wastewater treatment plants from the sanitary system of the Brazilian Federal District were assessed in 2018 and 2019. Samples were collected upstream and downstream from discharge points for each WWTP. Viral concentration based on adsorption-elution and conventional PCR was used for molecular detection, and positive samples were sequenced for phylogenetic analysis. Pluviosity data for the period in which the samples were collected were obtained. Our results demonstrated the presence of HAdVs in 27.2% (61/224) of the samples. The positivity was significantly higher in downstream samples compared to upstream. Moreover, the HAdV positivity was higher in downstream samples collected from receiving water bodies impacted by secondary-level WWTPs in comparison with those impacted by tertiary-level WWTPs. Phylogenetic analysis demonstrated the presence of genotypes 40 and 41, with prevalence of HAdV genotype 41. Despite the predominance of HAdV-41, an increasing frequency of the HAdV-40 was associated with higher pluviosity. In conclusion, this study is the first documentation in the Brazilian Federal District dealing with the prevalence and diversity of HAdVs in several WWTP, along with their correlation with rainfall index.

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Identification and Validation of Suitable Housekeeping Genes for Normalizing Quantitative Real-Time PCR Assays in Injured Peripheral Nerves

PLoS ONE ◽

10.1371/journal.pone.0105601 ◽

2014 ◽

Vol 9 (8) ◽

pp. e105601 ◽

Cited By ~ 17

Author(s):

Giovanna Gambarotta ◽

Giulia Ronchi ◽

Olivier Friard ◽

Pantaleo Galletta ◽

Isabelle Perroteau ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Peripheral Nerves ◽

Housekeeping Genes ◽

Quantitative Real Time Pcr ◽

Pcr Assays

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A cladistic revision of Tortopus Needham & Murphy with description of the new genus Tortopsis (Ephemeroptera: Polymitarcyidae)

Zootaxa ◽

10.11646/zootaxa.2481.1.1 ◽

2010 ◽

Vol 2481 (1) ◽

Author(s):

CARLOS MOLINERI

Keyword(s):

Phylogenetic Analysis ◽

Fore Wing ◽

Type Species ◽

New Genus ◽

Lateral View ◽

Median Line ◽

Male And Female ◽

Line Drawings ◽

Neotropical Species ◽

First Time

The 12 species previously placed in Tortopus together with 3 species newly described here, are revised and included in a phylogenetic analysis. Based on synapomorphic characters on the nymphs and adults of both sexes, Tortopus is restricted to T. igaranus Needham & Murphy, T. circumfluus Ulmer, T. harrisi Traver, T. zottai (Navás), T. bellus Lugo-Ortiz & McCafferty, and T. arenales sp. nov., and the genus is defined by: female parastyli receptors with long furrows anterior to sockets; penes entirely flattened; male ninth abdominal sternum almost separated in two portions by a median notch; mesosternum with furcasternal protuberances contiguous only on basal corner; and nymphs with two subapical tubercles on mandibular tusks. Tortopsis is newly described for T. bruchianus (Navás), T. limoncocha sp. nov., T. obscuripennis (Domínguez), T. parishi (Banks), T. primus (McDunnough), T. puella (Pictet), T. sarae (Domínguez), T. spatula sp. nov., and T. unguiculatus (Ulmer). Tortopsis is characterized by: R sector of female fore wing without additional veins between R 2 and IR; female parastyli receptors C or V-shaped, with sockets opening towards median line; male gonopore associated with a claw-like structure; penes separated from the base; parastyli more than 5 times length of pedestals; parastyli curved in lateral view; nymphs with a single subapical tubercle on mandibular tusks. The study of available type material permitted inclusion of comparative diagnoses, with figures and redescriptions as needed. The male imago of the type species of Tortopus (T. igaranus Needham & Murphy) is described for the first time, as are the female adults of Tortopus bellus Lugo-Ortiz & McCafferty and T. harrisi Traver. Three new Neotropical species based on male and female adults are described: Tortopus arenales and Tortopsis limoncocha from Ecuador, and Tortopsis spatula from Colombia. Keys to separate the adults and nymphs of the genera of Polymitarcyidae, and for male and female adults of all the species of Tortopus and Tortopsis are presented, as well as line drawings, pictures and SEM photographs of important structures.

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Complete Genome Sequence of Porcine Deltacoronavirus Strain CH/JXJGS01/2016, Isolated in Jiangxi Province, China, 2016

Genome Announcements ◽

10.1128/genomea.00832-17 ◽

2017 ◽

Vol 5 (34) ◽

Author(s):

Min Zhang ◽

Yu Ye ◽

Wang Gong ◽

Nannan Guo ◽

Fanfan Zhang ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Amino Acid ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Jiangxi Province ◽

Accession Number ◽

Chinese Strain

ABSTRACT The complete genome sequence of a variant of porcine deltacoronavirus, isolated from a diarrheal piglet and designated CH/JXJGS01/2016, was sequenced and analyzed. Phylogenetic analysis demonstrated that CH/JXJGS01/2016 shares the highest nucleotide and amino acid identities with the Chinese strain NH (GenBank accession number KU981059).

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