Estimation of dietary copper requirements of juvenile tilapia,Oreochromis niloticus5O. aureus

2003 ◽  
Vol 77 (2) ◽  
pp. 287-292 ◽  
Author(s):  
S.Y. Shiau ◽  
Y.C. Ning

AbstractAn 8-week feeding trial was conducted to determine the dietary copper (Cu) requirement of juvenile hybrid tilapia,Oreochromis niloticus5O. aureus. Purified diets with eight levels (0, 1, 2, 3, 4, 8, 12 or 20 mg Cu per kg diet) of supplemental Cu were given to tilapia (mean initial weight 0·79±0·03 g). Each diet was given to three replicate groups of fish. The rearing water contained 0·98 µg Cu per l. Weight gain was highest in fish given diets supplemented with 2 mg Cu per kg diet, followed by the group given 1 mg Cu per kg diet, then the unsupplemented control group, and was lowest in the 20 mg Cu per kg diet group. The differences between each of these four groups were significant (P< 0·05). Food efficiency (FE) of fish showed a similar trend to that of weight gain. Blood haemoglobin (Hb) and haematocrit (Hct) concentrations were lowest in fish given ≥ 12 mg Cu per kg diet, followed by fish given the unsupplemented control diet and highest in fish given 2 mg Cu per kg diet (Hb) and 2 and 3 mg Cu per kg diet (Hct). Plasma ceruloplasmin activity was higher in fish given 1 and 2 mg Cu per kg diet, followed by 3 to 12 mg Cu per kg diet and the control group, and lowest in fish given 20 mg Cu per kg diet. The body Cu content in fish generally increased as the dietary Cu supplementation level increased. Analysis by broken-line regression of weight gain percent and by linear regression of the whole-body Cu retention of fish indicated that the adequate dietary Cu concentration in growing tilapia is about 4 mg Cu per kg diet.

2016 ◽  
Vol 1 (3) ◽  
pp. 654-659 ◽  
Author(s):  
Ziaul Haque ◽  
Azimul Haque ◽  
Qin Pu ◽  
Ruijin Huang

A research work was undertaken to evaluate the feeding effect of tree forages on performance of growing sheep. Twenty growing sheep (in 4 groups) were fed three different tree forage diets. Tree forages Melia azardirachta, Leucaena leucocephala and Artocarpus heterophyllus were supplied in three treatments except one consisted no tree forages which was considered as control. There were significant (p<0.05) differences among the animal groups in terms of total DM and ME intake, although there were no significant differences in the digestibility of DM, OM, CP and ADF. Feeding of tree forages had significant (p<0.05) effect on live weight gain of sheep. Animals fed with tree forage based diets significantly increased weight gain (60.70 and 50.70 g/d for L. leucocephala and A. heterophyllus, respectively) compared to that (45.66 g/d) of control group fed with silage. Feed conversion efficiency (kg feed/kg gain) also showed that animals fed with tree forage of L. leucocephala and M. azardirachta based diets were having significantly (p<0.05) higher efficiency (11.33 and 11.47, respectively) compared to those of A. heterophyllus and control diet (13.77 and 13.33, respectively). The results of nitrogen balance of M. azardirachta and L. leucocephala tree forage had significantly (p<0.05) positive effect on the nitrogen retention in the body of the animals. Intake of all the tree forages was not similar which indicated that all of them were not accepted to the lamb in same level. So, diets with tree forages of L. leucocephala resulted better in terms of weight gain, digestibility and nitrogen balance compared to A. heterophyllu, M. azardirachta and silage (control).Asian J. Med. Biol. Res. December 2015, 1(3): 654-659


2008 ◽  
Vol 101 (6) ◽  
pp. 859-870 ◽  
Author(s):  
C. Bränning ◽  
Å. Håkansson ◽  
S. Ahrné ◽  
B. Jeppsson ◽  
G. Molin ◽  
...  

The aim was to investigate how blueberry husks and/or mixtures of probiotic strains (Lactobacillus crispatusDSM16743,L. gasseriDSM16737 andL. plantarumDSM15313 (LABmix), orBifidobacterium infantisDSM15159 and DSM15161 (BIFmix)) affect colonic fermentation, caecal counts of lactobacilli, bifidobacteria andEnterobacteriaceae, body weight gain, and blood concentrations of carboxylic acids (CA) and ammonia in rats. Dietary fibres in blueberry husks were fermented to 61 % in colon, and the elevated faecal excretion of fibre and protein contributed to the high faecal bulking capacity (1·3). The caecal pool of CA was higher in rats fed blueberry husks than the fibre-free control (P < 0·05), and the propionic acid proportion was higher in the distal colon than in the control group (P < 0·05). Probiotics lowered the caecal amount of CA when added to blueberry husks (P < 0·001), while the propionic acid proportion was higher with LABmix (P < 0·01) than blueberry husks only. The propionic acid and butyric acid concentrations in blood were higher in rats fed blueberry husks and probiotics than those fed blueberry husks only (P < 0·01), implying that the absorption of these acids was facilitated by the bacteria. The caecal counts of lactobacilli, bifidobacteria andEnterobacteriaceaewere lower in rats fed blueberry husks than the control diet (P < 0·05). The body weight gain was partly influenced by the caecal tissue and contents weights, and BIFmix decreased the ammonia concentration in blood (P < 0·05). We conclude that colonic fermentation is differentially affected by dietary fibre and probiotics, which may be of importance when developing foods with certain health effects.


2016 ◽  
Vol 1 (3) ◽  
pp. 648-653 ◽  
Author(s):  
Md Zillur Rahman ◽  
Md Yousuf Ali ◽  
Md Azharul Islam Talukder ◽  
Md Ershaduzzaman ◽  
Most Sumona Akter

A research work was undertaken to evaluate the feeding effect of tree forages on performance of growing sheep. Twenty growing sheep (in 4 groups) were fed three different tree forage diets. Tree forages Melia azardirachta, Leucaena leucocephala and Artocarpus heterophyllus were supplied in three treatments except one consisted no tree forages which was considered as control. There were significant (p<0.05) differences among the animal groups in terms of total DM and ME intake, although there were no significant differences in the digestibility of DM, OM, CP and ADF. Feeding of tree forages had significant (p<0.05) effect on live weight gain of sheep. Animals fed with tree forage based diets significantly increased weight gain (60.70 and 50.70 g/d for L. leucocephala and A. heterophyllus, respectively) compared to that (45.66 g/d) of control group fed with silage. Feed conversion efficiency (kg feed/kg gain) also showed that animals fed with tree forage of L. leucocephala and M. azardirachta based diets were having significantly (p<0.05) higher efficiency (11.33 and 11.47, respectively) compared to those of A. heterophyllus and control diet (13.77 and 13.33, respectively). The results of nitrogen balance of M. azardirachta and L. leucocephala tree forage had significantly (p<0.05) positive effect on the nitrogen retention in the body of the animals. Intake of all the tree forages was not similar which indicated that all of them were not accepted to the lamb in same level. So, diets with tree forages of L. leucocephala resulted better in terms of weight gain, digestibility and nitrogen balance compared to A. heterophyllu, M. azardirachta and silage (control).Asian J. Med. Biol. Res. December 2015, 1(3): 648-653


2001 ◽  
Vol 85 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Shi-Yen Shiau ◽  
Jia-Fen Hsieh

An 8 week feeding trial was conducted to determine the dietary K requirement for juvenile hybrid tilapia (Oreochromis niloticus × O. aureus). Purified diets with eight levels (0, 1, 2, 3, 4, 5, 7, 10 g/kg diet) of supplemental K were fed to tilapia. Each diet was fed to three replicate groups of fish initially weighing a mean value of 0.77 (SE 0.01) g/fish in a closed, recirculating rearing system. Weight gain was higher (P<0.05) in fish fed the diets supplemented with 2, 3 and 4 g K/kg diet than in fish fed diet with 10 g K/kg diet and the unsupplemented control diet. Gill Na+-K+ ATPase activity was highest in fish fed the diets supplemented with 1–3 g K/kg diet, followed by fish fed the diet with 5 g K/kg diet and lowest in fish fed the diet with 10 g K/kg diet. Whole-body K content in fish were generally increased as the dietary K supplementation level increased. Analysis by polynomial regression of weight gain and gill Na+-K+ ATPase activity and by linear regression of whole-body K retention of the fish indicated that the adequate dietary K concentration for tilapia is about 2–3 g/kg diet.


2004 ◽  
Vol 91 (4) ◽  
pp. 585-590 ◽  
Author(s):  
Shi-Yen Shiau ◽  
Li-Shan Lu

Two 8-week feeding trials were conducted to determine the dietary Na requirement for juvenile hybrid tilapia (Oreochromis niloticus × O. aureus) reared in fresh water and seawater. In each experiment, NaCl was added to the basal diet at 0, 0·5, 1, 2, 3, 5, or 7g Na/kg diet (fresh water) and at 0, 0·2, 0·5, 0·8, 1·2, 1·5, 2, or 3g Na/kg diet (seawater). Each diet was fed to three replicate groups of fish, individual fish initially weighing 0·69 (se 0·01) g, in a closed, recirculating rearing system. In fresh water, the tilapia fed the diet supplemented with 2g Na/kg diet had significantly (P<0·05) greater weight gain than the fish fed the diets supplemented with ≥3 and ≤0·5g Na/kg diet. Feed efficiency (FE) in fish generally followed the weight-gain pattern. Gill Na+–K+ ATPase activity was highest in the fish fed the diets supplemented with 1–3g Na/kg diet, followed by the fish fed the diet with 7g Na/kg diet and lowest in the fish fed the unsupplemented control diet. In seawater, the weight gain, FE and gill Na+–K+ ATPase activity in fish were not affected by the dietary treatment. Analysis by polynomial regression of weight gain, by broken-line regression of gill Na+–K+ ATPase activity and by linear regression of whole-body Na retention of the fish reared in fresh water, indicated that the adequate dietary Na concentration for tilapia is about 1·5g/kg diet. The present study also suggests that no dietary Na is required for tilapia reared in seawater.


2020 ◽  
Vol 17 (35) ◽  
pp. 800-812
Author(s):  
Ilgiz DOLININ ◽  
George BAZEKIN ◽  
Evgeny SKOVORODIN ◽  
Almaz SHARIPOV ◽  
Ivan CHUDOV

Poultry farming holds a special place in ensuring the products that the consumers demand, it provides the population with essential food products,such as eggs and meat,that contain vital micro and macronutrients, proteins, lipids, and vitamins. Therefore, the issues of rational, economically feasible feeding of meat poultry, namely broiler chickens, are an urgent task. It is also essential to find effective methods of their application in order to correct the natural resistance and immune and biological reactivity of birds. The purpose of this research is to study the effect of the biological stimulant-Nucleostim on the growth and development of chickens, hematological, and immunological parameters of the blood of birds.This Biostimulant is a purified bovine spleen extract containing at least 1 mg / ml of low molecular weight peptides (nucleotides and nucleosides) formed as a result of autolysis, using dry whey and diatomite as fillers. Onthe application ofNucleostim, the gain in live weight of chickens was increased by 9.7%. At the end of the experiment, the livability of the chicks of the experimental group treated with Nucleostimcame up to 88%, compared with the 72% of the control group. The use of biostimulant had a stimulating effect on the liver of chickens confirmed by the research results presented in the article, as well as contributed to the development of the thymus in the setting of general dystrophy. Thus, it improved chicklivability and increased body weight gain. The biological stimulant-Nucleostim as an adaptogenic, anabolic, and immunostimulatory agent is promising for finding new drugs that improve the health and productivity of poultry.


Rangifer ◽  
2000 ◽  
Vol 20 (4) ◽  
pp. 187 ◽  
Author(s):  
A. Nilsson ◽  
Ö. Danell ◽  
M. Murphy ◽  
K. Olsson ◽  
B. Åhman

The transition from experimentally induced poor nutritional conditions to feeding was studied with 69 eight-month-old female reindeer (Rangifer tarandus tarandus). During a pre-experimental period, all reindeer were fed a simulated winter diet with 80% lichens Cladina spp. and 20% Vaccinum myrtillus shrubs and Salix spp. leaves (lichen diet) ad lib. The reindeer were divided into five groups. A control group (group C) was fed the lichen diet ad lib. throughout the experiment. Four groups were fed half of that ration for eight days and were then totally deprived of feed for one day (restriction period). During the following 34 days (feeding period) the groups were re-fed the lichen diet (group L), fed pelleted reindeer feed combined with either lichen (group PL) or grass silage (group PS), or fed silage with a gradually increasing addition of pellets (group SP). Weekly measurements of blood samples and body weighr showed that the control group remained clinically healthy and had stable blood plasma concentrations of protein, urea, glucose and insulin throughout the experiment, but they lost weight. At slaughter, before and after the restriction period, all animals had lost rumen-free body weight, but the reindeer fed a restricted amount of feed lost more than the control group. Also the plasma metabolites were affected by the restricted feeding, with increased concentrations of urea and decreased concentrations of glucose. Group L responded immediately to the ad lib. feeding with blood metabolite levels rapidly approaching those of group C. The body weight developments were similar in groups L and C. Although the feed rations were increased gradually, diarrhoea occurred in some animals belonging to groups PL and PS within the first week of the feeding period. All reindeer recovered, after antibiotic treatment of the worst affected animals. The PL and PS groups, which had high contents of metabolisable energy and crude protein in their diets, showed increased con-centtations of plasma protein, urea and insulin. At the end of the feeding period, these groups had increased their body and carcass weights and gained fat, whereas reindeer fed the lichen diet had lost weight. Severe health problems (malnutrition and so-called wet belly) occurred in group SP during the first weeks of feeding and led to loss of animals, and consequently the SP group was excluded from the remainder of rhe experiment. The general conclusion is that the lichen diet did not cause any digestive problems, but resulted in a continuous decline in body weight and small or deficient fat reserves. After the initial diarrhoea, feeding with diets comprising pellets from the start resulted in improved condition, expressed as increased body weight, fat gain and higher concentrations of plasma protein, urea and insulin in relation to the control group. The diet initially based on grass in the form of silage of the given quality seemed insufficient as feed to reindeer calves in a poor nutritional state.


1980 ◽  
Vol 43 (1) ◽  
pp. 205-215 ◽  
Author(s):  
Mary H. Stevenson ◽  
N. Jackson

1. A cereal-based diet containing 7.6 mg copper/kg was fed ad lib. to laying hens for up to 48 d. Four other groups were given the control diet to which was added hydrated copper sulphate to provide 250, 500, 1000 and 2000 mg added Cu/kg.2. Hens were killed on day o and after 3, 6, 12, 24 and 48 d. Records were kept of body-weight, food consumption, egg production and egg weight.3. After slaughter blood haemoglobin, packed cell volume, serum Cu and aspartate aminotransfferase- (AAT; EC 2.6. I. I) were measured. The liver, kidneys, a sample of breast muscle, oviduct, ovary and gizzard were weighed. Gizzard, spleen. liver and kidney tissue were examined histologically.4. The Cu, zinc and iron concentrations of liver, kidneys and breast muscle and the manganese concentrations of liver and kidneys were determined.5. Body-weight loss occurred at 500–2000mg added Cu/kg diet. Egg production was depressed by level of added Cu and period of time on the Cu-containing diets.6. Mean liver, kidney, oviduct and ovarian weights per unit body-weight were depressed by Cu in the diet and the effect increased with period of time on the diets. Mean gizzard weight per unit body-weight was increased by dietary added Cu and by time.7. Cu concentrations in the liver were increased by dietary level of added Cu and period of timeon the diet. Zn concentration in liver increased at 1000 and 2000 mg added Cu/kg diet and liver Fe concentration was increased at these levels. Histological examination of the gizzard indicated that the Cu content of the gizzard lining increased with dietary added Cu.


2007 ◽  
Vol 98 (5) ◽  
pp. 937-943 ◽  
Author(s):  
Claudia Di Giacomo ◽  
Rosaria Acquaviva ◽  
Andrea Piva ◽  
Valeria Sorrenti ◽  
Luca Vanella ◽  
...  

The aim of the present study was to verify whether the oral administration of cyanidin 3-O-β-d-glucoside (C3G) might counteract damage induced by chronic exposure (28 d) to ochratoxin A (OTA) in rats and if its effect may be mediated by haeme oxygenase-1 (HO-1). Forty male Sprague–Dawley rats, individually caged, were divided into four groups of ten animals. A control group received a commercial diet, group C3G received the control diet supplemented with C3G (1 g/kg feed), group OTA received the control diet supplemented with 200 parts per billion of OTA, and group OTA+C3G received the OTA group diet supplemented with C3G (1 g/kg feed). After 4 weeks of treatment animals were killed and the liver, kidneys and brain of each rat were collected and homogenised to evaluate non-proteic thiol groups (RSH), lipid hydroperoxide (LOOH) levels, HO-1 expression and DNA fragmentation. Rats of the OTA group showed a significant (P < 0·001) decrease in RSH content of kidney and liver and a significant (P < 0·001) increase of LOOH in all the examined tissues compared with the control group. In the OTA+C3G group both RSH content and LOOH levels were similar to those observed in the control group, demonstrating that C3G was able to counteract the effects of OTA. A significant (P < 0·001) induction of HO-1 was evident in kidney and liver of both OTA and C3G groups. DNA damage occurred in all the examined tissues of the OTA group, whereas C3G was able to prevent it. The present study confirmed that the effects of OTA are mediated by oxidative stress and demonstrated that C3G efficiently counteracted deleterious effects of OTA because of its antioxidant and HO-1-inducing properties.


1984 ◽  
Vol 52 (2) ◽  
pp. 205-213 ◽  
Author(s):  
Susan J. Fairweather-Tait ◽  
A. J. A. Wright ◽  
Christine M. Williams

1. Pregnant rats were given control (46 mg iron/kg, 61 mg zinc/kg), low-Zn (6.9 mg Zn/kg) or low-Zn plus Fe (168 mg Fe/kg) diets from day 1 of pregnancy. The animals were allowed to give birth and parturition times recorded. Exactly 24 h after the end of parturition the pups were killed and analysed for water, fat, protein, Fe and Zn contents and the mothers' haemoglobin (Hb) and packed cell volume (PCV) were measured.2. There were no differences in weight gain or food intakes throughout pregnancy. Parturition times were similar (mean time 123 (SE 15) min) and there were no differences in the number of pups born. Protein, water and fat contents of the pups were similar but the low-Zn Fe-supplemented group had higher pup Fe than the low-Zn unsupplemented group, and the control group had higher pup Zn than both the low-Zn groups. The low-Zn groups had a greater incidence of haemorrhaged or deformed pups, or both, than the controls.3. Pregnant rats were given diets of adequate Zn level (40 mg/kg) but with varying Fe:Zn (0.8, 1.7, 2.9, 3.7). Zn retention from the diet was measured using 65Zn as an extrinsic label on days 3, 10 and 17 of pregnancy with a whole-body gamma-counter. A group of non-pregnant rats was also included as controls. The 65Zn content of mothers and pups was measured 24–48 h after birth and at 14, 21 and 24 d of age.4. In all groups Zn retention was highest from the first meal, fell in the second meal and then rose in the third meal of the pregnant but not the non-pregnant rats. There were no differences between the groups given diets of varying Fe:Zn level. Approximately 25% of the 65Zn was transferred from the mothers to the pups by the time they were 48 h old, and a further 17% during the first 14 d of lactation. The pup 65Zn content did not significantly increase after the first 20 d of lactation but the maternal 65Zn level continued to fall gradually.


Sign in / Sign up

Export Citation Format

Share Document