The effects of wheat variety on diet digestibility and on performance parameters of pigs from 10 - 20 kg

1999 ◽  
Vol 1999 ◽  
pp. 166-166 ◽  
Author(s):  
F.J. Lewis ◽  
H. Schulze ◽  
J. McEvoy ◽  
K.J McCracken

Lewiset al(1998) observed differences in overall and ileal apparent digestibility of wheat varieties using a wheat / casein diet but no significant relationships within vitroviscosity of wheat. In the present study six of the varieties used previously were selected to examine if the variety differences would be repeatable using a more commercial diet and the extent of correlation between overall digestibility (OD) and performance parameters. The wheats chosen wereAbbott, Caxton, Crofter, Encore, Hussar, Riband(in vitroviscosity range 6.8 - 19.2 cps). Three contained the 1B1R gene (Crofter, Encore, Hussar) and three were soft wheats (Crofter, Encore, Riband). All wheats were harvested in Northern Ireland in 1997.

1999 ◽  
Vol 1999 ◽  
pp. 165-165 ◽  
Author(s):  
F.J. Lewis ◽  
M.E.E. McCann ◽  
H. Schulze ◽  
J. McEvoy ◽  
K.J McCracken

Thermal processing is carried out on commercial diets primarily to kill bacteria and reduce mould growth, although additional benefits have been shown such as an inactivation of trypsin inhibitors present in soyabean meal (McNaughton and Reece, 1980). However, heat treatment if too severe can produce negative effects, such as reduced apparent digestibility and increased viscosity of gut contents in broilers, an effect reversed by the addition of enzymes (McCracken et al, 1993). The aim of this study was to examine the effect of heat treatment during diet preparation on ileal and overall apparent digestibility of diets for growing pigs, made from two wheat varieties of different in vitro viscosity (8.8 vs 20.8 cps) with and without the addition of feed enzyme.


Author(s):  
Muhammad Zahaib Ilyas ◽  
Muhammad Roman ◽  
Muhammad Waqas Ali ◽  
Muhammad Nadeem

High antioxidants level in food is gradually becoming popular because of the enhanced risk of oxidative stress in humans. Bread wheat is rich in vital antioxidants but its major bioactive compounds are not available for the human. This study was conducted with the aim to enhance the phytochemical constituents and antioxidative activity of wheat grains by fermenting it with the use of Bacillus subtilis KCTC 13241. The antioxidative potential was determined by DPPH (2,2-diphenyl-1-picryl- hydrazyl) and ABTS (3-ethyl-benzothiazo- line-6-sulfonic acid) radical scavenging assay as well by the concentration of amino acids, flavonoids, minerals, carbohydrates, and phenolic compounds. Different varieties showed different free radical scavenging potential on fermentation, which was significantly high with respect to their corresponding unfermented wheat varieties. The highest potential was found in a fermented wheat variety named Namhae and this combination can be used in the pharmaceutical and food industries.


2016 ◽  
Vol 40 (4) ◽  
pp. 475-483
Author(s):  
José Cardoso de Araújo Neto ◽  
Fernando Yuri Brandão Fernandes ◽  
José Carlos Machado Pimentel ◽  
Vânia Rodrigues Vasconcelos ◽  
Márcia Mourão Ramos Azevedo

ABSTRACT The growing consumption of green coconut - fresh and industrialized - in Brazil generates a large volume of wastes and coproducts that justifies the search for alternatives for their use in animal feeding. The most limiting factor to the inclusion of these coproducts in ruminant diets is their high fiber content, which may restrict intake and performance. The present study determined the composition and in vitro dry matter degradability of whole coconut, coconut powder, and coconut fiber treated with sodium hydroxide (0%, 3%, and 6%) and the effect of including whole coconut hydrolyzed with 6% sodium hydroxide at different concentrations (25%, 30%, 35%, and 40%) in the diet on apparent digestibility of nutrients, performance, and feeding behavior of sheep. Alkalinization decreased the hemicellulose, NDF, and ADF contents of the evaluated coproducts. Whole coconut and coconut powder provided greater in vitro degradation of DM compared with coconut fiber, with highest values obtained with the inclusion of 6% sodium hydroxide. Inclusion of whole coconut in the diets did not affect the intakes of DM, CP, NDF, and ADF, but influenced weight gain, feed conversion, and apparent digestibility of DM and NDF. Regarding the feeding behavior of the animals, the inclusion of whole coconut in the diet only affected their rumination time. Whole coconut hydrolyzed with 6% NAOH can be included in diets for feedlot sheep at up to 35% without reducing intake or weight gain.


1991 ◽  
Vol 52 (3) ◽  
pp. 461-464 ◽  
Author(s):  
E. R. Ørskov ◽  
G. W. Reid ◽  
M. Kay

ABSTRACTThree types of straw were used, a spring barley (variety Corgi) untreated (UC), ammonia-treated Corgi (AC) and an ammonia-treated winter wheat (variety Longbow) (AW). Each straw was included in a complete diet containing either 350 (L), 450 (M) or 550 (H) g/kg straw. The other principal ingredients were rolled barley, molassed sugar-beet pulp and fish meal. Hereford cross steers approximately 16 months old and weighing 350 kg live weight were given the diets ad libitum in both a main trial lasting 80 days, to assess intakes, live-weight gains and food efficiency, and in a subsidiary trial to measure in vivo apparent digestibility.The live-weight gains during 80 days for steers given the UC diet at L, M and H inclusion were 117, 102 and 0·70 kg/day respectively. The respective live-weight gains for AC were 1·32, 1·27 and 1 02 kg/day and for AW 1 ·26, 1 ·20 and 0·95 kg/day.While experimental comparisons were terminated at 80 days when the first steers were slaughtered, all except five with the lowest growth rate reached the target fat class for slaughter before 6 months on the diets. While differences in intake and performance between steers given the straws closely reflected differences in degradation characteristics determined by in sacco measurements, the increase in in vivo apparent digestibility between untreated (0·510) and treated (0·545) was small. It is concluded that a large proportion of high quality or treated straw can be incorporated and utilized in diets for finishing cattle.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 360-361 ◽  
Author(s):  
Tom N Dale ◽  
Tim Parr ◽  
Julie King ◽  
John Brameld

Abstract The study objective was to investigate the effects of in-vitro fibrolytic enzyme incubation on xylooligosaccharide (XOS) release from different wheat varieties. Six varieties of wheat were ground to a 300µm powder and subjected to total non-cellulosic hydrolysis with Trifluroacetic acid (TFA) and in-vitro incubations with one of three commercially available enzymes at their recommended doses: Econase XT (xylanase 160,000 BXU/g), Econase MP1000 (Mannase 100,000 MNU/g) or Econase BP700 (ß-glucanase 700,000 BU/g) or no added enzymes. Each wheat variety was suspended (at 5mg/ml) in 50mM sodium citrate buffer (pH 5.2) with or without the individual enzymes (n = 3), and incubated at 40.7°C in a shaking incubator. Samples were taken at 0 and 6hr. Concentrations of xylose, xylobiose and xylotriose were determined by High-Performance Anion-Exchange Chromatography coupled with Pulsed Electrochemical Detection fitted with a CarboPac PA20 (xylose) or CarboPac PA200 Column (Dionex, Thermo Scientific) and known xylose and XOS standards (Megazyme, Ltd). Data were analysed by one or two-way ANOVA (Genstat 19th Edition), with significance accepted at P < 0.05. After TFA hydrolysis, there were significant differences between varieties (P < .001, ANOVA) in total xylose contents (Huntsman > Paragon > Chinese spring > Sinuelo > Highbury = Pavon 74). In the in-vitro incubations, there was a significant effect of enzyme, but not wheat variety, on both xylose (P = 0.009) and xylotriose (P < .001) release at 6 hours, with Econase XT releasing more than the other two enzymes. There was a significant enzyme x wheat variety interaction (P < .001) for xylobiose release at 6 hours, with Econase MP1000 releasing the most from Huntsman. In conclusion, the 6 wheat varieties differed in their non-cellulosic xylose contents and there were clear differences in the amounts of XOS released by the 3 enzymes. Further trials are needed to investigate whether the in-vitro experiments are indicative of results observed in-vivo.


2004 ◽  
Vol 15 (3) ◽  
pp. 246-246
Author(s):  
M.A. Tony ◽  
A. Butschke ◽  
J. Zagon ◽  
H. Broll ◽  
M. Schauzu ◽  
...  

Author(s):  
Ewa A. Burian ◽  
Lubna Sabah ◽  
Klaus Kirketerp-Møller ◽  
Elin Ibstedt ◽  
Magnus M. Fazli ◽  
...  

Acute wounds may require cleansing to reduce the risk of infection. Stabilized hypochlorous acid in acetic buffer (HOCl + buffer) is a novel wound irrigation solution with antimicrobial properties. We performed a first-in-man, prospective, open-label pilot study to document preliminary safety and performance in the treatment of acute wounds. The study enrolled 12 subjects scheduled for a split-skin graft transplantation, where the donor site was used as a model of an acute wound. The treatment time was 75 s, given on 6 occasions. A total of 7 adverse events were regarded as related to the treatment; all registered as pain during the procedure for 2 subjects. One subject had a wound infection at the donor site. The mean colony-forming unit (CFU) decreased by 41% after the treatment, and the mean epithelialization was 96% on both days 14 (standard deviation [SD] 8%) and 21 (SD 10%). The study provides preliminary support for the safety, well-tolerance, and efficacy of HOCl + buffer for acute wounds. The pain was frequent although resolved quickly. Excellent wound healing and satisfying antimicrobial properties were observed. A subsequent in vitro biofilm study also indicated good antimicrobial activity against Pseudomonas aeruginosa with a 96% mean reduction of CFU, when used for a treatment duration of 15 min ( P < .0001), and a 50% decrease for Staphylococcus aureus ( P = .1010). Future larger studies are needed to evaluate the safety and performance of HOCl + buffer in acute wounds, including the promising antimicrobial effect by prolonged treatment on bacterial biofilms.


Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 710
Author(s):  
Tanja Ilić ◽  
Ivana Pantelić ◽  
Snežana Savić

Due to complex interdependent relationships affecting their microstructure, topical semisolid drug formulations face unique obstacles to the development of generics compared to other drug products. Traditionally, establishing bioequivalence is based on comparative clinical trials, which are expensive and often associated with high degrees of variability and low sensitivity in detecting formulation differences. To address this issue, leading regulatory agencies have aimed to advance guidelines relevant to topical generics, ultimately accepting different non-clinical, in vitro/in vivo surrogate methods for topical bioequivalence assessment. Unfortunately, according to both industry and academia stakeholders, these efforts are far from flawless, and often upsurge the potential for result variability and a number of other failure modes. This paper offers a comprehensive review of the literature focused on amending regulatory positions concerning the demonstration of (i) extended pharmaceutical equivalence and (ii) equivalence with respect to the efficacy of topical semisolids. The proposed corrective measures are disclosed and critically discussed, as they span from mere demands to widen the acceptance range (e.g., from ±10% to ±20%/±25% for rheology and in vitro release parameters highly prone to batch-to-batch variability) or reassess the optimal number of samples required to reach the desired statistical power, but also rely on specific data modeling or novel statistical approaches.


Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 186
Author(s):  
Maria Natalia Calienni ◽  
Daniela Maza Vega ◽  
C. Facundo Temprana ◽  
María Cecilia Izquierdo ◽  
David E. Ybarra ◽  
...  

Vismodegib is a first-in-class inhibitor for advanced basal cell carcinoma treatment. Its daily oral doses present a high distribution volume and several side effects. We evaluated its skin penetration loaded in diverse nanosystems as potential strategies to reduce side effects and drug quantities. Ultradeformable liposomes, ethosomes, colloidal liquid crystals, and dendrimers were able to transport Vismodegib to deep skin layers, while polymeric micelles failed at this. As lipidic systems were the most effective, we assessed the in vitro and in vivo toxicity of Vismodegib-loaded ultradeformable liposomes, apoptosis, and cellular uptake. Vismodegib emerges as a versatile drug that can be loaded in several delivery systems for topical application. These findings may be also useful for the consideration of topical delivery of other drugs with a low water solubility.


Chemosensors ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 54
Author(s):  
Eun-Song Lee ◽  
Jeong Min Lee ◽  
Hea-Jin Kim ◽  
Young-Pil Kim

Aptamers are single-stranded DNA or RNA molecules that can be identified through an iterative in vitro selection–amplification process. Among them, fluorogenic aptamers in response to small molecules have been of great interest in biosensing and bioimaging due to their rapid fluorescence turn-on signals with high target specificity and low background noise. In this review, we report recent advances in fluorogenic aptasensors and their applications to in vitro diagnosis and cellular imaging. These aptasensors modulated by small molecules have been implemented in different modalities that include duplex or molecular beacon-type aptasensors, aptazymes, and fluorogen-activating aptamer reporters. We highlight the working principles, target molecules, modifications, and performance characteristics of fluorogenic aptasensors, and discuss their potential roles in the field of biosensor and bioimaging with future directions and challenges.


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