scholarly journals Regulating role of abscisic acid on cadmium enrichment in ramie (Boehmeria nivea L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kunmei Chen ◽  
Ping Chen ◽  
Xiaojun Qiu ◽  
Jikang Chen ◽  
Gang Gao ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Chlorophyll Content ◽  
Plant Stress ◽  
Metal Resistance ◽  
Culture Solution ◽  
Cd Uptake ◽  
Boehmeria Nivea ◽  
Regulated Expression ◽  
Aba Content ◽  
The Relationship

AbstractAbscisic acid (ABA) is known as an important hormone regulating plant stress resistance, such as salt, drought and heavy metal resistance. However, the relationship between ABA and cadmium (Cd) enrichment in ramie (Boehmeria nivea L.) is still unclear to date. This study aimed to reveal the effect of ABA on Cd enrichment in ramie, and we received the following results: (1) Under Cd treatment, the Cd uptake of ramie increased with the increase of Cd concentration, but the chlorophyll content decreased. Under Cd treatment, the ABA content was highest in roots of ramie, followed by that in old leaves, and lowest in new leaves. Long-time treatment of high Cd concentration reduced the ability of endogenous ABA biosynthesis. (2) Spraying ABA on ramie plants (SORP) and adding ABA directly to the culture solution (ADCS) with low concentration can promote the growth of ramie and increase the amount of Cd uptake, and the effect of SORP is better. (3) The molecular reason for the decrease of chlorophyll content due to Cd stress, may be resulted from the down-regulated expression of the chlorophyll synthesis genes (BnPAO and BnNYC1) and the up-regulated expression of the chlorophyll degradation genes (BnCHLH, BnCHLG, BnHAP3A and BnPPR1). The elevated ABA content in ramie plants may due to the up-regulated expression of the ABA synthesis related genes (BnABA1, BnNCED3, and BnNCED5) and the genes (BnABCG40, BnNFXL2, BnPYL9, BnGCR2, BnGTG1, BnBGLU1, BnUTG1, BnVHAG1 and BnABI5) that encoding ABA transport and response proteins, which was consistent with the enhance the Cd uptake in ramie. Our study revealed the relationship between ABA and Cd uptake in ramie, which provided a reference for improving the enrichment of Cd in ramie.

Abscisic acid content and components of water status in leaves of Populus deltoides

1986 ◽  
Vol 64 (10) ◽  
pp. 2295-2298 ◽  
Author(s):  
Tsai-Yun Lin ◽  
Edward Sucoff ◽  
Mark Brenner
Keyword(s):  
Abscisic Acid ◽  
Populus Deltoides ◽  
Water Status ◽  
Leaf Water ◽  
Leaf Water Status ◽  
Eastern Cottonwood ◽  
Detached Leaves ◽  
Aba Content ◽  
The Relationship ◽  
Abscisic Acid Content

The relationship between abscisic acid (ABA) and leaf water status was studied during the air drying of detached leaves of eastern cottonwood (Populus deltoides Marsh.). The ABA content increased exponentially as leaf water potential and leaf turgor potential decreased. No clearly defined thresholds were observed between ABA content and these variables. ABA content was linearly related to the relative fresh weight and was not related to the osmotic potential.

scholarly journals Expression of AhATL1, an ABA Transport Factor Gene from Peanut, Is Affected by Altered Memory Gene Expression Patterns and Increased Tolerance to Drought Stress in Arabidopsis

2021 ◽  
Vol 22 (7) ◽  
pp. 3398
Author(s):  
Ming Qin ◽  
Xiaoyan Li ◽  
Shaohua Tang ◽  
Yinglin Huang ◽  
Ling Li ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Stress Responses ◽  
Expression Patterns ◽  
Plant Stress ◽  
Limited Information ◽  
Response Gene ◽  
Stress Memory ◽  
Gene Type ◽  
Aba Content

Arachis hypogaea abscisic acid transporter like-1 (AhATL1) modulates abscisic acid (ABA) sensitivity by specifically influencing the importing of ABA into cells, and is a key player in plant stress responses. However, there is limited information on ABA transporters in crops. In this study, we found that the level of AhATL1 expression and AhATL1 distribution increased more rapidly in the second drought (D2) compared with in the first drought (D1). Compared with the first recovery (R1), the AhATL1 expression level and ABA content remained at a higher level during the second recovery (R2). The heterologous overexpression of AhATL1 in Arabidopsis changed the expression pattern of certain memory genes and changed the post response gene type into the memory gene type. Regarding the proline and water content of Col (Arabidopsis thaliana L. Heynh., Col-0), atabcg22, and AhATL1-OX during drought training, the second drought (D2) was more severe than the first drought (D1), which was more conducive to maintaining the cell osmotic balance and resisting drought. In summary, drought stress memory resulted in a rapid increase in the AhATL1 expression and AhATL1 distribution level, and then raised the endogenous ABA content and changed the post response gene type into the memory gene type, which enhanced the drought resistance and recovery ability.

scholarly journals Maize ZmbZIP33 Is Involved in Drought Resistance and Recovery Ability Through an Abscisic Acid-Dependent Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Liru Cao ◽  
Xiaomin Lu ◽  
Guorui Wang ◽  
Qianjin Zhang ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Chlorophyll Content ◽  
Signaling Pathway ◽  
Drought Resistance ◽  
Aba Signaling ◽  
Maize Leaves ◽  
Aba Content ◽  
Aba Biosynthesis ◽  
Dependent Signaling Pathway

Analyzing the transcriptome of maize leaves under drought stress and rewatering conditions revealed that transcription factors were involved in this process, among which ZmbZIP33 of the ABSCISIC ACID-INSENSITIVE 5-like protein 5 family was induced to significantly up-regulated. The functional mechanism of ZmbZIP33 in Abscisic acd (ABA) signaling pathway and its response to drought stress and rewatering has not been studied yet. The present study found that ZmbZIP33 contains a DNA-binding and dimerization domain, has transcriptional activation activity, and is highly homologous to SbABI1,SitbZIP68 and OsABA1. The expression of ZmbZIP33 is strongly up-regulated by drought, high salt, high temperature, and ABA treatments. Overexpression of ZmbZIP33 remarkably increased chlorophyll content and root length after drought stress and rewatering, and, moreover, cause an accumulation of ABA content, thereby improving drought resistance and recovery ability in Arabidopsis. However, silencing the expression of ZmbZIP33 (BMV-ZmbZIP33) remarkably decreased chlorophyll content, ABA content, superoxide dismutase and peroxidase activities, and increased stomatal opening and water loss rate compared with BMV (control). It showed that silencing ZmbZIP33 lead to reduced drought resistance and recovery ability of maize. ABA sensitivity analysis found that 0.5 and 1 μmol/L treatments severely inhibited the root development of overexpression ZmbZIP33 transgenic Arabidopsis. However, the root growth of BMV was greatly inhibited for 1 and 5μmol/L ABA treatments, but not for BMV-ZmbZIP33. Subcellular localization, yeast two-hybrid and BIFC further confirmed that the core components of ABA signaling pathways ZmPYL10 and ZmPP2C7 interacted in nucleus, ZmPP2C7 and ZmSRK2E as well as ZmSRK2E and ZmbZIP33 interacted in the plasma membrane. We also found that expression levels of ZmPYL10 and ZmSRK2E in the BMV-ZmbZIP33 mutant were lower than those of BMV, while ZmPP2C7 was the opposite under drought stress and rewatering. However, expression of ZmPYL10 and ZmSRK2E in normal maize leaves were significantly up-regulated by 3–4 folds after drought and ABA treatments for 24 h, while ZmPP2C7 was down-regulated. The NCED and ZEP encoding key enzymes in ABA biosynthesis are up-regulated in overexpression ZmbZIP33 transgenic line under drought stress and rewatering conditions, but down-regulated in BMV-ZmbZIP33 mutants. Together, these findings demonstrate that ZmbZIP33 played roles in ABA biosynthesis and regulation of drought response and rewatering in Arabidopsis and maize thought an ABA-dependent signaling pathway.

scholarly journals Effects of Cultivar and Ethanol Disinfection on Aseptic Germination of Loquat (Eriobotrya japonica) Seeds

HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 941-945
Author(s):  
Kunkun Li ◽  
Weiqi Dong ◽  
Yun Zhao ◽  
Hongxia Xu ◽  
Junwei Chen ◽  
...  
Keyword(s):  
Detrimental Effect ◽  
Germination Rate ◽  
Fruit Tree ◽  
Final Concentration ◽  
Eriobotrya Japonica ◽  
Seedling Height ◽  
Disinfection Procedure ◽  
Aba Content ◽  
Subtropical Fruit ◽  
The Relationship

Loquat (Eriobotrya japonica Lindl.) is an economically important subtropical fruit tree, originating and commercially produced mainly in China and Japan. The aseptic seed germination of 13 loquat cultivars, including 9 white-fleshed cultivars and 4 red-fleshed ones, was studied, and the relationship between the germination rate and the content of endogenous ABA in seeds was explored. The germination rate and the seedling height at 80 days after sowing of white-fleshed cultivars were generally higher than that of red-fleshed ones. The ABA content in seeds was generally lower in white-fleshed cultivars, and the ABA content negatively correlated with the germination rate at 80 days after sowing. A moderate detrimental effect of 75% ethanol disinfection on aseptic germination of loquat seeds was observed, especially, for the cultivar Dahongpao, and the germination rate and seedling height were reduced by around half and one-third, respectively. The addition of plant preservative mixture (PPM) to the germination medium at a final concentration of 25 mg·L−1 effectively controlled contamination when the 75% ethanol disinfection procedure was omitted.

scholarly journals The relationship between SPAD chlorophyll and disease severity index in Ganoderma-infected oil palm seedlings

2019 ◽  
Vol 17 (3) ◽  
pp. 355-358 ◽  
Author(s):  
M.R.M. Rakib ◽  
A.H. Borhan ◽  
A.N. Jawahir
Keyword(s):  
Chlorophyll Content ◽  
Disease Severity ◽  
Oil Palm ◽  
Single Photon ◽  
Linear Trend ◽  
Severity Index ◽  
Quadratic Model ◽  
Wood Block ◽  
Disease Severity Index ◽  
The Relationship

Establishment of disease in oil palm seedlings through artificial inoculation of Ganoderma are widely used for studies of various aspects of plant pathology, including epidemiology, etiology, disease resistance, host-parasite interaction and disease control. The estimation of chlorophyll content in the infected seedlings possibly could provide a good indicator for degree of disease or infection, and changes during pathogenesis. Thus, the objective of this study was to evaluate the relationship between disease severity index (DSI) and chlorophyll content in Ganoderma infected oil palm seedlings. Three-month-old oil palm seedlings were infected with Ganoderma inoculum on rubber wood block (RWB), where 44 isolates of Ganoderma were tested. Disease severity index (DSI) and chlorophyll content using a single-photon avalanche diode (SPAD) meter were recorded at 4 weeks interval for a period of 24 weeks after inoculation (WAI). Pearson's correlation analysis and regression analysis were performed to evaluate the relationship between the variables. It was found that the relationship between DSI and SPAD chlorophyll value was inversely proportional (R = -0.92) in a linear trend (R2 = 0.85). Furthermore, the increasing trend of the DSI across the weeks were fitted in a quadratic model (R2 = 0.99). In contrast, the SPAD chlorophyll value declined in a linear trend (R2 = 0.98). The SPAD chlorophyll value could be considered as a better alternative over the DSI as the SPAD chlorophyll value was strongly related to DSI, as well as able to detect physiological changes in the infected oil palm seedlings at the early stages of pathogenesis. J Bangladesh Agril Univ 17(3): 355–358, 2019

scholarly journals Initiation and Execution of Programmed Cell Death and Regulation of Reactive Oxygen Species in Plants

2021 ◽  
Vol 22 (23) ◽  
pp. 12942
Author(s):  
Chanjuan Ye ◽  
Shaoyan Zheng ◽  
Dagang Jiang ◽  
Jingqin Lu ◽  
Zongna Huang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Plant Stress ◽  
Reactive Oxygen ◽  
Signalling Pathways ◽  
Oxygen Species ◽  
Signalling Molecules ◽  
Plant Stress Tolerance ◽  
The Relationship

Programmed cell death (PCD) plays crucial roles in plant development and defence response. Reactive oxygen species (ROS) are produced during normal plant growth, and high ROS concentrations can change the antioxidant status of cells, leading to spontaneous cell death. In addition, ROS function as signalling molecules to improve plant stress tolerance, and they induce PCD under different conditions. This review describes the mechanisms underlying plant PCD, the key functions of mitochondria and chloroplasts in PCD, and the relationship between mitochondria and chloroplasts during PCD. Additionally, the review discusses the factors that regulate PCD. Most importantly, in this review, we summarise the sites of production of ROS and discuss the roles of ROS that not only trigger multiple signalling pathways leading to PCD but also participate in the execution of PCD, highlighting the importance of ROS in PCD.

Differential accumulation of abscisic acid and its catabolites in drought-sensitive and drought-tolerant sunflower seeds

2009 ◽  
Vol 19 (4) ◽  
pp. 201-211 ◽  
Author(s):  
Andrea Andrade ◽  
Ana Vigliocco ◽  
Sergio Alemano ◽  
Daniel Alvarez ◽  
Guillermina Abdala
Keyword(s):  
Abscisic Acid ◽  
No Reduction ◽  
Water Environment ◽  
Embryonic Axis ◽  
Sunflower Seeds ◽  
Maternal Plant ◽  
Drought Tolerant ◽  
Field Lines ◽  
Aba Content ◽  
Germinated Seeds

AbstractEleven sunflower (Helianthus annuus L.) inbred lines were evaluated in field and laboratory studies under drought and irrigation. In the field, lines B59, R419 and B67 had reduced seed and oil yield under drought, while no reduction was observed for R432, HAR4 and B71. Lines HA89, R415, R049, RHA274 and R423 presented intermediate responses. In laboratory tests, seeds of line B59 had reduced germination percentages at 200 and 400 mM mannitol, while germination of seeds of lines R432, B71, HAR4, RHA274 and HA89 was reduced only at 400 mM mannitol. Drought-sensitive B59 and drought-tolerant B71 grown under irrigation and drought conditions in the field were selected for hormone assays. Abscisic acid (ABA) and its catabolites in pericarp, embryonic axis and cotyledons of dry and germinated seeds of B59 and B71 were determined. ABA was the major component of the pericarp of dry seeds from B71 and B59 plants grown under drought. The embryonic axis of B71 dry seeds from drought-grown plants also showed high ABA content. The major findings from this study are: (1) the drought-sensitive and -tolerant lines exhibited different ABA and catabolite profiles; (2) water environment during maternal plant growth affected ABA content and the composition of catabolites in mature and germinated seeds; (3) ABA content did not affect germination performance in our conditions; and (4) the dry and germinated seed parts showed different ABA and catabolite profiles.

Changes in abscisic acid content during and after drought are related to carbohydrate mobilization and hydraulic recovery in poplar stems

2020 ◽  
Vol 40 (8) ◽  
pp. 1043-1057 ◽  
Author(s):  
Cecilia Brunetti ◽  
Tadeja Savi ◽  
Andrea Nardini ◽  
Francesco Loreto ◽  
Antonella Gori ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Hydraulic Conductivity ◽  
Stomatal Closure ◽  
Populus Nigra ◽  
Plant Responses ◽  
Stem Water Potential ◽  
Transport Efficiency ◽  
Drought Relief ◽  
Aba Content ◽  
Abscisic Acid Content

Abstract Drought compromises plant's ability to replace transpired water vapor with water absorbed from the soil, leading to extensive xylem dysfunction and causing plant desiccation and death. Short-term plant responses to drought rely on stomatal closure, and on the plant's ability to recover hydraulic functioning after drought relief. We hypothesize a key role for abscisic acid (ABA) not only in the control of stomatal aperture, but also in hydraulic recovery. Young plants of Populus nigra L. were used to investigate possible relationships among ABA, non-structural carbohydrates (NSC) and xylem hydraulic function under drought and after re-watering. In Populus nigra L. plants subjected to drought, water transport efficiency and hydraulic recovery after re-watering were monitored by measuring the percentage loss of hydraulic conductivity (PLC) and stem specific hydraulic conductivity (Kstem). In the same plants ABA and NSC were quantified in wood and bark. Drought severely reduced stomatal conductance (gL) and markedly increased the PLC. Leaf and stem water potential, and stem hydraulic efficiency fully recovered within 24 h after re-watering, but gL values remained low. After re-watering, we found significant correlations between changes in ABA content and hexoses concentration both in wood and bark. Our findings suggest a role for ABA in the regulation of stem carbohydrate metabolism and starch mobilization upon drought relief, possibly promoting the restoration of xylem transport capacity.

Inhibition of Flowering in Lolium temulentum L. By Water Stress: a Role for Abscisic Acid

1977 ◽  
Vol 4 (2) ◽  
pp. 225 ◽  
Author(s):  
RW King ◽  
LT Evans
Keyword(s):  
Abscisic Acid ◽  
Water Stress ◽  
Long Day ◽  
Relative Water ◽  
The One ◽  
Floral Evocation ◽  
Leaf Photosynthetic Rate ◽  
Response To Water ◽  
Aba Content ◽  
High Intensity Light

A brief, 8-h water stress during the induction of flowering in L. temulentum reduces the flowering response, the more so the greater the stress. Water stress also affected leaf photosynthetic rate, relative water content of leaves and leaf elongation. Water stress was most inhibitory to flowering when applied during the period of high-intensity light at the beginning of the one long day. The abscisic acid (ABA) content of leaves increased up to 30-fold during the imposition of water stress and fell rapidly after stress was relieved, regardless of when the stress was imposed. The greater the stress, the higher was the level of ABA in leaves and the greater was the inhibition of flowering. The ABA content of apices also rose in response to water stress, in some cases during the stress treatment but usually 8-22 h later. Flowering was inhibited when apical ABA contents were high at the end of the long day. Although water stress may influence the flowering of plants in several ways, these experiments suggest that water stress during the long day induction of L. temulentum inhibits flowering by raising the content of ABA at the shoot apex during floral evocation.

Sign in / Sign up

Export Citation Format

Share Document