scholarly journals Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Osama A. Madkhali ◽  
Sivakumar Sivagurunathan Moni ◽  
Muhammad H. Sultan ◽  
Haitham A. Bukhary ◽  
Mohammed Ghazwani ◽  
...  
Keyword(s):  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
Antibacterial Properties ◽  
Xrd Analysis ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
Antibacterial Effects ◽  
Scanning Calorimetry ◽  
E Coli ◽  
Antibacterial Studies

AbstractThe purpose of this study was to develop a novel nano antibacterial formulation of dextran sulfate sodium polymer. The dextran sulfate sodium (DSS) nanoparticles were formulated with gelation technique. The nanoparticles exhibited significant physicochemical and effective antibacterial properties, with zeta potential of − 35.2 mV, particle size of 69.3 z d nm, polydispersity index of 0.6, and percentage polydispersity of 77.8. The DSS nanoparticles were stable up to 102 °C. Differential scanning calorimetry revealed an endothermic peak at 165.77 °C in 12.46 min, while XRD analysis at 2θ depicted various peaks at 21.56°, 33.37°, 38.73°, 47.17°, 52.96°, and 58.42°, indicating discrete nanoparticle formation. Antibacterial studies showed that the DSS nanoparticles were effective against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations of DSS nanoparticles for Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae) and Proteus vulgaris (P. vulgaris) were 150, 200, 250, 150, 200, 250, 250 µg/mL, respectively. The antibacterial effects of DSS nanoparticles were in the order E. coli (26 ± 1.2 mm) at 150 µg/mL > S. pyogenes (24.6 ± 0.8 mm) at 250 µg/mL > B. subtilis (23.5 ± 2 mm) at 150 µg/mL > K. pneumoniae (22 ± 2 mm) at 250 µg/mL > P. aeruginosa (21.8 ± 1 mm) at 200 µg/mL > S. aureus (20.8 ± 1 mm) at 200 µg/mL > P. vulgaris (20.5 ± 0.9 mm) at 250 µg/mL. These results demonstrate the antibacterial potency of DSS injectable nanoparticles.

scholarly journals Functional and structural basis of E. coli enolase inhibition by SF2312: a mimic of the carbanion intermediate

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jolanta Krucinska ◽  
Michael N. Lombardo ◽  
Heidi Erlandsen ◽  
Akram Hazeen ◽  
Searle S. Duay ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Antibacterial Properties ◽  
High Energy ◽  
Structural Basis ◽  
Gram Negative Bacteria ◽  
E Coli ◽  
Promising Target ◽  
Antibiotic Discovery ◽  
Growth And Reproduction

AbstractMany years ago, the natural secondary metabolite SF2312, produced by the actinomycete Micromonospora, was reported to display broad spectrum antibacterial properties against both Gram-positive and Gram-negative bacteria. Recent studies have revealed that SF2312, a natural phosphonic acid, functions as a potent inhibitor of human enolase. The mechanism of SF2312 inhibition of bacterial enolase and its role in bacterial growth and reproduction, however, have remained elusive. In this work, we detail a structural analysis of E. coli enolase bound to both SF2312 and its oxidized imide-form. Our studies support a model in which SF2312 acts as an analog of a high energy intermediate formed during the catalytic process. Biochemical, biophysical, computational and kinetic characterization of these compounds confirm that altering features characteristic of a putative carbanion (enolate) intermediate significantly reduces the potency of enzyme inhibition. When SF2312 is combined with fosfomycin in the presence of glucose-6 phosphate, significant synergy is observed. This suggests the two agents could be used as a potent combination, targeting distinct cellular mechanism for the treatment of bacterial infections. Together, our studies rationalize the structure-activity relationships for these phosphonates and validate enolase as a promising target for antibiotic discovery.

scholarly journals Bioactivity and Antibacterial Behaviors of Nanostructured Lithium-Doped Hydroxyapatite for Bone Scaffold Application

2021 ◽  
Vol 22 (17) ◽  
pp. 9214 ◽  
Author(s):  
Pardis Keikhosravani ◽  
Hossein Maleki-Ghaleh ◽  
Amir Kahaie Khosrowshahi ◽  
Mahdi Bodaghi ◽  
Ziba Dargahi ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Xrd Analysis ◽  
Bone Scaffold ◽  
Cell Analysis ◽  
Cellular Behavior ◽  
E Coli ◽  
Li Doping ◽  
Cold Pressed ◽  
And Staphylococcus Aureus ◽  
Li Content

The material for bone scaffold replacement should be biocompatible and antibacterial to prevent scaffold-associated infection. We biofunctionalized the hydroxyapatite (HA) properties by doping it with lithium (Li). The HA and 4 Li-doped HA (0.5, 1.0, 2.0, 4.0 wt.%) samples were investigated to find the most suitable Li content for both aspects. The synthesized nanoparticles, by the mechanical alloying method, were cold-pressed uniaxially and then sintered for 2 h at 1250 °C. Characterization using field-emission scanning electron microscopy (FE-SEM) revealed particle sizes in the range of 60 to 120 nm. The XRD analysis proved the formation of HA and Li-doped HA nanoparticles with crystal sizes ranging from 59 to 89 nm. The bioactivity of samples was investigated in simulated body fluid (SBF), and the growth of apatite formed on surfaces was evaluated using SEM and EDS. Cellular behavior was estimated by MG63 osteoblast-like cells. The results of apatite growth and cell analysis showed that 1.0 wt.% Li doping was optimal to maximize the bioactivity of HA. Antibacterial characteristics against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were performed by colony-forming unit (CFU) tests. The results showed that Li in the structure of HA increases its antibacterial properties. HA biofunctionalized by Li doping can be considered a suitable option for the fabrication of bone scaffolds due to its antibacterial and unique bioactivity properties.

scholarly journals Antibacterial Properties of Nanosilver PLLA Fibrous Membranes

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Lin Li ◽  
Yi Li ◽  
Jiashen Li ◽  
Lei Yao ◽  
Arthur F. T. Mak ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Weight Ratio ◽  
Antibacterial Effects ◽  
E Coli ◽  
Fibrous Membranes ◽  
Composite Fibrous Membranes

Nanosilver has been studied as a valuable material for it strong antibacterial effects. In this study, we investigated the antibacterial properties of nano silver Poly-L-Lactic acid (Ag/PLLA) composite fibrous membranes. Ag/PLLA fibrous membranes were prepared with silver nanoparticles having weight ratio of silver nanoparticles to PLLA at 5% (w/w). In vitro antibacterial tests were performed usingEscherichia coli(E. coli) andStaphylococcus aureus(Staph.) to determine the antibacterial capability of the Ag/PLLA fibrous membranes. As the results suggested, Ag/PLLA fibrous membranes showed strong antibacterial properties. Thus, Ag/PLLA fibrous membrane can be used as an antibacterial scaffold for tissue engineering.

scholarly journals A Mini Review of Antibacterial Properties of ZnO Nanoparticles

2021 ◽  
Vol 9 ◽  
Author(s):  
Sergey V. Gudkov ◽  
Dmitriy E. Burmistrov ◽  
Dmitriy A. Serov ◽  
Maxim B. Rebezov ◽  
Anastasia A. Semenova ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
World Health ◽  
Oxide Nanoparticles ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Antibacterial Potential

The development of antibiotic resistance of bacteria is one of the most pressing problems in world health care. One of the promising ways to overcome microbial resistance to antibiotics is the use of metal nanoparticles and their oxides. In particular, numerous studies have shown the high antibacterial potential of zinc oxide nanoparticles (ZnO-NP) in relation to gram-positive and gram-negative bacteria. This mini-review includes an analysis of the results of studies in recent years aimed at studying the antibacterial activity of nanoparticles based on zinc oxide. The dependence of the antibacterial effect on the size of the applied nanoparticles in relation to E. coli and S. aureus is given. The influence of various ways of synthesis of zinc oxide nanoparticles and the main types of modifications of NP-ZnO to increase the antibacterial efficiency are also considered.

scholarly journals Antibacterial properties of quercetin

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Renu Narendra Jaisinghani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Lactobacillus Casei ◽  
Shigella Flexneri ◽  
Antibacterial Properties ◽  
Dilution Method ◽  
Proteus Vulgaris ◽  
E Coli ◽  
Broth Dilution

Quercetin is a polyphenolic flavonoid with potential chemoprotective properties. In the present work its antibacterial properties were studied against Staphylococcus aureus, Escherichia coli, Shigella flexneri, Proteus vulgaris, Pseudomonas aeruginosa, Lactobacillus casei var shirota by broth dilution method. Quercetin inhibited S. aureus, P. aeruginosa at concentration 20 mcg/mL while P. vulgaris and E. coli were inhibited at concentration 300 mcg/mL and 400 mcg/mL respectively. Shigella flexneri and Lactobacillus casei var shirota were completely indifferent even at concentration of 500 mcg/mL.

scholarly journals Editing of the gut microbiota reduces carcinogenesis in mouse models of colitis-associated colorectal cancer

2019 ◽  
Vol 216 (10) ◽  
pp. 2378-2393 ◽  
Author(s):  
Wenhan Zhu ◽  
Naoteru Miyata ◽  
Maria G. Winter ◽  
Alexandre Arenales ◽  
Elizabeth R. Hughes ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Gut Microbiota ◽  
Chronic Inflammation ◽  
Mouse Models ◽  
Dextran Sulfate ◽  
Dextran Sulfate Sodium ◽  
E Coli ◽  
Gut Colonization ◽  
Gut Microbiota Dysbiosis ◽  
Colitis Model

Chronic inflammation and gut microbiota dysbiosis, in particular the bloom of genotoxin-producing E. coli strains, are risk factors for the development of colorectal cancer. Here, we sought to determine whether precision editing of gut microbiota metabolism and composition could decrease the risk for tumor development in mouse models of colitis-associated colorectal cancer (CAC). Expansion of experimentally introduced E. coli strains in the azoxymethane/dextran sulfate sodium colitis model was driven by molybdoenzyme-dependent metabolic pathways. Oral administration of sodium tungstate inhibited E. coli molybdoenzymes and selectively decreased gut colonization with genotoxin-producing E. coli and other Enterobacteriaceae. Restricting the bloom of Enterobacteriaceae decreased intestinal inflammation and reduced the incidence of colonic tumors in two models of CAC, the azoxymethane/dextran sulfate sodium colitis model and azoxymethane-treated, Il10-deficient mice. We conclude that metabolic targeting of protumoral Enterobacteriaceae during chronic inflammation is a suitable strategy to prevent the development of malignancies arising from gut microbiota dysbiosis.

Preparation of Nano Cu-ZnO/PET Fiber for Antibacterial Application

2017 ◽  
Vol 898 ◽  
pp. 2272-2278 ◽  
Author(s):  
Jin Jin ◽  
Ling Jie Fang ◽  
Lian Tang ◽  
Peng Ji ◽  
Chao Sheng Wang ◽  
...  
Keyword(s):  
Melt Spinning ◽  
Low Cost ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
High Strength ◽  
Melting Behavior ◽  
Polyester Fiber ◽  
Scanning Electron Micrographs ◽  
Scanning Calorimetry ◽  
E Coli

Polyethylene terephthalate (PET) fiber is applied to clothing, home textiles and other fields because of its low cost, high strength, quick-drying and stable structure, etc. Polyester fiber generally doesn’t have the ability to kill bacteria, and it is a kind of porous material which is conducive to the microbial adhesion and spread of bacteria. In the present study, Cu-ZnO and its antibacterial PET fibers were prepared by sol–gel method and melt spinning, respectively. The structures and compositions of Cu-ZnO were characterized by X-ray diffraction (XRD) and Fourier transform-infrared (FT-IR) spectra. The crystallization and melting behavior of the PET/Cu-ZnO (0.05:1) composites were tested by differential scanning calorimetry (DSC) to determine the temperate of melt spinning. The morphologies and mechanical properties of antibacterial polyester fiber were studied by field emission scanning electron micrographs (FE-SEM) and strength machine, respectively. The antibacterial properties of the samples on E. coli and S. aureus were determined using powder inhibition zones and antibacterial rate. The results show that the fibers exhibited excellent antibacterial activity, it had obvious inhibiting effects on E. coli and S. aureus and the antibacterial rate were both above 90%.

scholarly journals The growth influence of Escherichia coli co-cultured with other selected gram negative bacteria

2019 ◽  
Vol 27 ◽  
pp. 101-108
Author(s):  
OA Oyewole ◽  
A Hamidu ◽  
IO Egbewole ◽  
R Adewole ◽  
OE Oladoja
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Salmonella Typhi ◽  
Shigella Dysenteriae ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
Macconkey Agar ◽  
Gram Negative ◽  
E Coli ◽  
Positive Growth

This study examined the influence of Escherichia coli on the growth of other selected Gram negative bacteria (Klebsiella pneumoniae, Shigella dysenteriae, Salmonella typhi, Pseudomonas aeruginosa and Proteus vulgaris). Cultures of each bacterium at 0, 24, 48 and 72 hours of incubation were plated on MacConkey agar. Colonies that developed were counted while the optical densities were determined at 0, 24, 48 and 72 hours using spectrophotometry. Each bacterium was co-cultured with E. coli and their growth was determined using culturing method and spectrophotometry. The result showed an increase in growth in the cultures of each isolate co-cultured with E. coli when compared with single bacterium culture with the exception of P. aeruginosa. The result of this study revealed a positive growth influence between E. coli and K. pneumoniae, S. dysenteriae, S. typhi, and P. vulgaris, except for P. aeruginosa that showed a decrease in growth. J. bio-sci. 27: 101-108, 2019

scholarly journals Chemical Composition and Antimicrobial Activity of Ethanolic Bark and Leave Extract of Zanthoxylum Caribaeum Lam. from Norte de Santander, Colombia

2020 ◽  
Vol 72 (4) ◽  
pp. 152-161
Author(s):  
Oscar Ortega-Buitrago ◽  
Giovanni Chaves-Bedoya ◽  
Luz Yineth Ortiz-Rojas
Keyword(s):  
Biological Activity ◽  
Antibacterial Properties ◽  
Bioactive Molecules ◽  
Gram Negative Bacteria ◽  
Microbial Diseases ◽  
Reduced Pressure ◽  
E Coli ◽  
Potential Source ◽  
Diffusion Technique ◽  
Antibacterial And Antifungal

Interest in the study of bioactive molecules present in plant organs such as root, leaves, stems or flowers, has increased significantly in search of applications in fields such as medicine and agriculture, mainly due to the bactericidal, antifungal and insecticides properties found in different plant species. In this study, leaf and bark extracts obtained at reduced pressure of Z. caribaeum were evaluated for their antibacterial and antifungal activity using the Kirby-Bauer agar disk diffusion technique. We found that extracts from the bark of this tree show a greater biological activity compared to extracts obtained from leaf, mainly against Grampositive bacteria such as S. aureus and S. mutans. However, there was no significant biological activity against gram-negative bacteria such as E. coli or Morganella sp. The composition of the extract determined by gas chromatography coupled to mass spectrophotometry (GC-MS), reveal the presence of the compound 3.5-dihydroxy-6-methyl-2.3-dihydro-4H-piran-one (32.8%) as a majority compound, which has been reported with antibacterial properties. The biological activity of the extracts of Z. caribeaum represents a potential source for the development of drugs for the control of microbial diseases.

scholarly journals Purification of IHF-like protein from gram-negative bacteria in one chromatographic step.

1996 ◽  
Vol 43 (2) ◽  
pp. 379-382 ◽  
Author(s):  
B Krawczyk ◽  
J Kur
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Integration Host Factor ◽  
Gram Negative Bacteria ◽  
Proteus Vulgaris ◽  
Gram Negative ◽  
Acinetobacter Junii ◽  
E Coli ◽  
Alpha Beta

We describe a fast and very efficient method of purification which yields highly purified integration host factor-like proteins in one chromatographic step. IHF-like proteins from Acinetobacter junii or Proteus vulgaris are each an alpha beta heterodimer (subunits of 10 and 11 kDa) similar to the IHF of Escherichia coli when analyzed by polyacrylamide gel electrophoresis. The purified IHF are able to bind to the same ihf sites as IHF of E. coli. The results presented confirm that IHF is conserved during evolution in gram-negative bacteria.

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