Hybrid membrane biomaterials from self-assembly in polysaccharide and peptide amphiphile mixtures: controllable structural and mechanical properties and antimicrobial activity

Macroscopic capsules, with tunable properties based on hierarchical self-assembly on multiple lengthscales, are prepared from the co-operative self-assembly of polysaccharide and peptide amphiphiles.

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A “Light-up” 1D supramolecular nanoprobe for silver ions based on assembly of pyrene-labeled peptide amphiphiles: cell-imaging and antimicrobial activity

Journal of Materials Chemistry B ◽

10.1039/c4tb00892h ◽

2014 ◽

Vol 2 (38) ◽

pp. 6478-6486 ◽

Cited By ~ 8

Author(s):

Inhye Kim ◽

Heon-Ho Jeong ◽

Yong-Jae Kim ◽

Na-Eun Lee ◽

Kang-moo Huh ◽

...

Keyword(s):

Antimicrobial Activity ◽

Self Assembly ◽

Rational Design ◽

Cell Imaging ◽

Antimicrobial Agents ◽

Fluorescence Enhancement ◽

Silver Ions ◽

Peptide Amphiphiles ◽

Potential Use

The histidine-coated fibrils response to Ag+ with fluorescence enhancement was developed through a rational design based on the aqueous self-assembly of peptides for potential use as cell-imaging and antimicrobial agents.

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Molecular Design of Peptide Amphiphiles for Controlled Self-Assembly and Drug Release

Journal of Materials Chemistry B ◽

10.1039/d1tb00173f ◽

2021 ◽

Author(s):

Ziqi Liu ◽

Xuan Tang ◽

Feng Feng ◽

Jing Xu ◽

Can Wu ◽

...

Keyword(s):

Drug Delivery ◽

Drug Release ◽

Self Assembly ◽

Molecular Design ◽

Drug Dose ◽

Peptide Amphiphiles ◽

Great Promise ◽

Specific Drug ◽

Peptide Amphiphile ◽

Drug Delivery Applications

Peptide amphiphile-based supramolecular hydrogels hold great promise in drug delivery applications. To cater for specific drug dose in a demanding biomedical scenario, sophisticated design of peptide amphiphile (PA) molecule is...

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Nanostructure, Self-Assembly, Mechanical Properties, and Antioxidant Activity of a Lupin-Derived Peptide Hydrogel

Biomedicines ◽

10.3390/biomedicines9030294 ◽

2021 ◽

Vol 9 (3) ◽

pp. 294

Author(s):

Raffaele Pugliese ◽

Anna Arnoldi ◽

Carmen Lammi

Keyword(s):

Mechanical Properties ◽

Antioxidant Activity ◽

Self Assembly ◽

Antioxidant Activities ◽

Functional Materials ◽

Cd Spectroscopy ◽

Beneficial Effects ◽

Naturally Occurring ◽

New Research ◽

Peptide Hydrogel

Naturally occurring food peptides are frequently used in the life sciences due to their beneficial effects through their impact on specific biochemical pathways. Furthermore, they are often leveraged for applications in areas as diverse as bioengineering, medicine, agriculture, and even fashion. However, progress toward understanding their self-assembling properties as functional materials are often hindered by their long aromatic and charged residue-enriched sequences encrypted in the parent protein sequence. In this study, we elucidate the nanostructure and the hierarchical self-assembly propensity of a lupin-derived peptide which belongs to the α-conglutin (11S globulin, legumin-like protein), with a straightforward N-terminal biotinylated oligoglycine tag-based methodology for controlling the nanostructures, biomechanics, and biological features. Extensive characterization was performed via Circular Dichroism (CD) spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), rheological measurements, and Atomic Force Microscopy (AFM) analyses. By using the biotin tag, we obtained a thixotropic lupin-derived peptide hydrogel (named BT13) with tunable mechanical properties (from 2 to 11 kPa), without impairing its spontaneous formation of β-sheet secondary structures. Lastly, we demonstrated that this hydrogel has antioxidant activity. Altogether, our findings address multiple challenges associated with the development of naturally occurring food peptide-based hydrogels, offering a new tool to both fine tune the mechanical properties and tailor the antioxidant activities, providing new research directions across food chemistry, biochemistry, and bioengineering.

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Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications

International Journal of Molecular Sciences ◽

10.3390/ijms22010105 ◽

2020 ◽

Vol 22 (1) ◽

pp. 105

Author(s):

Wanting Li ◽

Zixuan Huang ◽

Rui Cai ◽

Wan Yang ◽

Huawei He ◽

...

Keyword(s):

Mechanical Properties ◽

Antimicrobial Activity ◽

Composite Film ◽

Water Absorption ◽

Rational Design ◽

Hybrid Nanoparticles ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

X Ray

Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents. Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity. Based on a layer-by-layer self-assembly strategy, AgNPs and ZnO were immobilized on sericin-agarose films using the adhesion property of polydopamine. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively. Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films. In addition, the antibacterial activity of the composite film was evaluated against Gram-positive and Gram-negative bacteria. The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria. It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.

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Incorporation of Hybrid Nanomaterial in Dental Porcelains: Antimicrobial, Chemical, and Mechanical Properties

Antibiotics ◽

10.3390/antibiotics10020098 ◽

2021 ◽

Vol 10 (2) ◽

pp. 98

Author(s):

Carla L. Vidal ◽

Izabela Ferreira ◽

Paulo S. Ferreira ◽

Mariana L. C. Valente ◽

Ana B. V. Teixeira ◽

...

Keyword(s):

Mechanical Properties ◽

Fracture Toughness ◽

Antimicrobial Activity ◽

Biofilm Formation ◽

Silver Ions ◽

Surface Characteristics ◽

Streptococcus Sobrinus ◽

Hybrid Nanomaterial ◽

Antimicrobial Substances ◽

Health Area

Biofilm formation on biomaterials is a challenge in the health area. Antimicrobial substances based on nanomaterials have been proposed to solve this problem. The aim was to incorporate nanostructured silver vanadate decorated with silver nanoparticles (β-AgVO3) into dental porcelains (IPS Inline and Ex-3 Noritake), at concentrations of 2.5% and 5%, and evaluate the surface characteristics (by SEM/EDS), antimicrobial activity (against Streptococcus mutans, Streptococcus sobrinus, Aggregatibacter actinomycetemcomitans, and Pseudomonas aeruginosa), silver (Ag+) and vanadium (V4+/V5+) ions release, and mechanical properties (microhardness, roughness, and fracture toughness). The β-AgVO3 incorporation did not alter the porcelain’s components, reduced the S. mutans, S. sobrinus and A. actinomycetemcomitans viability, increased the fracture toughness of IPS Inline, the roughness for all groups, and did not affect the microhardness of the 5% group. Among all groups, IPS Inline 5% released more Ag+, and Ex-3 Noritake 2.5% released more V4+/V5+. It was concluded that the incorporation of β-AgVO3 into dental porcelains promoted antimicrobial activity against S. mutans, S. sobrinus, and A. actinomycetemcomitans (preventing biofilm formation), caused a higher release of vanadium than silver ions, and an adequate mechanical behavior was observed. However, the incorporation of β-AgVO3 did not reduce P. aeruginosa viability and increased the surface roughness of dental porcelains.

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Microenvironment pH-Induced Selective Cell Death for Potential Cancer Therapy Using Nanofibrous Self-Assembly of a Peptide Amphiphile

Biomacromolecules ◽

10.1021/acs.biomac.1c00267 ◽

2021 ◽

Author(s):

Shota Yamamoto ◽

Kanon Nishimura ◽

Kenta Morita ◽

Sayuki Kanemitsu ◽

Yuki Nishida ◽

...

Keyword(s):

Cell Death ◽

Cancer Therapy ◽

Self Assembly ◽

Peptide Amphiphile ◽

Potential Cancer ◽

Microenvironment Ph

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ChemInform Abstract: The Importance of the Self-Assembly Process to Control Mechanical Properties of Low Molecular Weight Hydrogels

ChemInform ◽

10.1002/chin.201336240 ◽

2013 ◽

Vol 44 (36) ◽

pp. no-no

Author(s):

Jaclyn Raeburn ◽

Andre Zamith Cardoso ◽

Dave J. Adams

Keyword(s):

Mechanical Properties ◽

Molecular Weight ◽

Self Assembly ◽

The Self ◽

Low Molecular Weight ◽

Assembly Process

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Self-assembly of Stimuli Responsive Coiled-coil Fibrous Hydrogels

Soft Matter ◽

10.1039/d1sm00780g ◽

2021 ◽

Author(s):

Michael Meleties ◽

Priya Katyal ◽

Bonnie Lin ◽

Dustin Britton ◽

Jin Kim Montclare

Keyword(s):

Drug Delivery ◽

Tissue Engineering ◽

Self Assembly ◽

Coiled Coil ◽

Stimuli Responsive ◽

Tunable Properties ◽

Stimuli Sensitive ◽

Biomedical Fields

Owing to their tunable properties, hydrogels comprised of stimuli sensitive polymers are one of the most appealing scaffolds with applications in tissue engineering, drug delivery and other biomedical fields. We...

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