scholarly journals Raman observation of a molecular signaling pathway of apoptotic cells induced by photothermal therapy

2019 ◽  
Vol 10 (47) ◽  
pp. 10900-10910 ◽  
Author(s):  
Yingfang Xing ◽  
Zhewei Cai ◽  
Meijuan Xu ◽  
Wenzheng Ju ◽  
Xiaojun Luo ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Signaling Pathway ◽  
Photothermal Therapy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Apoptotic Cells ◽  
Molecular Signaling ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Molecular Signaling Pathway

A molecular signaling pathway of apoptosis induced by photothermal therapy was revealed by surface-enhanced Raman spectroscopy.

scholarly journals NIMG-76. SURFACE-ENHANCED RAMAN SPECTROSCOPY (SERS) FOR INTRAOPERATIVE BRAIN TUMOR IMAGING AND PHOTOTHERMAL THERAPY

2017 ◽  
Vol 19 (suppl_6) ◽  
pp. vi159-vi159 ◽  
Author(s):  
Hamed Arami ◽  
Edwin Chang ◽  
Chirag B Patel ◽  
Steven James Madsen ◽  
Ryan Miller Davis ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Brain Tumor ◽  
Photothermal Therapy ◽  
Tumor Imaging ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Investigation of dual plasmonic core-shell Ag@CuS nanoparticles for potential surface-enhanced Raman spectroscopy-guided photothermal therapy

Nanomedicine ◽  
2021 ◽  
Author(s):  
Anindita Das ◽  
Vinothini Arunagiri ◽  
Hsieh-Chih Tsai ◽  
Adhimoorthy Prasannan ◽  
Juin-Yih Lai ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Photothermal Therapy ◽  
Heat Dissipation ◽  
Near Infrared ◽  
Surface Enhanced Raman Spectroscopy ◽  
Infrared Light ◽  
Core Shell ◽  
Theoretical Simulation ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Aim: To prepare efficient metal-semiconductor nanoparticles as noninvasive, real-time imaging probes for photothermal therapy (PTT) applications. Materials & methods: A bottom-up approach was used to fabricate core-shell Ag@CuS nanoparticles (NPs). PTT and Raman mapping were done using HeLa cells. Theoretical simulation of electric field enhancement and heat dissipation density of Ag@CuS NPs was performed. Results: PTT-induced hyperthermia was achieved under 940 nm near-infrared light irradiation. Surface-enhanced Raman spectroscopy (SERS) signals of dye molecules were observed when conjugated with Ag@CuS NPs. Conclusion: Ag@CuS NPs are found to be efficient for SERS imaging and localized heating under laser irradiation, making a promising candidate for SERS-guided PTT.

scholarly journals Molecular Detection of Cordycepin-Induced HeLa Cell Apoptosis with Surface-Enhanced Raman Spectroscopy

2019 ◽  
Vol 9 (19) ◽  
pp. 3990
Author(s):  
Pei Ma ◽  
Lei Xu ◽  
Luyao Wang ◽  
Nan Chen ◽  
Xuedian Zhang ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Hela Cell ◽  
Cell Apoptosis ◽  
Ribosomal Proteins ◽  
Morphological Changes ◽  
Surface Enhanced Raman Spectroscopy ◽  
Apoptotic Cells ◽  
Label Free ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Cordycepin, extracted from the medicinal mushroom Cordyceps militaris, was shown to induce cancer cell apoptosis. Yet its anticancer function was limitedly evaluated, and the mechanism was not entirely elucidated. In this study, we demonstrated the effectiveness of cordycepin in inducing apoptosis in HeLa cells and investigated its apoptosis-inducing mechanism through label-free surface-enhanced Raman spectroscopy (SERS). SERS spectral changes revealed detailed molecular changes in both early and late stages of apoptosis. Importantly, SERS characteristic peaks at 805 and 1438 cm−1, which were assigned to RNA, continued to decrease significantly from early to late apoptosis stages. It indicated that cordycepin induced HeLa cell apoptosis mainly through interfering with RNA production, potentially by restraining the translation of RNA encoding ribosomal proteins. Meanwhile, apoptotic cells and their apoptosis stage could be easily differentiated by SERS-based principal component analysis (PCA). Furthermore, the morphological changes of early and late-stage apoptotic cells were illustrated by differential interference contrast and fluorescence microscopic imaging. Therefore, the natural ingredient, cordycepin, could serve as a promising anticancer candidate. Our biological model of cell apoptosis in vitro, the non-invasive and rapid SERS technique, combined with PCA analysis, could be a powerful tool in the investigation of cell apoptosis mechanisms and anticancer drug screening.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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