scholarly journals Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity

RSC Advances ◽  
2020 ◽  
Vol 10 (65) ◽  
pp. 39954-39966
Author(s):  
Yang Wang ◽  
Wei Wang ◽  
Zhun Gu ◽  
Xiangyang Miao ◽  
Qiuyan Huang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Sod Activity ◽  
Temperature Responsive ◽  
Chelation Reaction

Fe(ii)-based nanozymes (FeCPNGs) have been successfully prepared by a chelation reaction and presented peroxidase and SOD activity.

Evaluation of Azadirachtin on Arthrospira plantensis Gomont growth parameters and antioxidant enzymes

2020 ◽  
Vol 56 ◽  
pp. 8
Author(s):  
Hatice Tunca ◽  
Ali Doğru ◽  
Feray Köçkar ◽  
Burçin Önem ◽  
Tuğba Ongun Sevindik
Keyword(s):  
Enzyme Activity ◽  
Growth Parameters ◽  
Arthrospira Platensis ◽  
Proline Content ◽  
Enzyme Analysis ◽  
Free Proline ◽  
Sod Activity ◽  
Pesticide Pollution ◽  
Antioxidant Parameters ◽  
Mda Content

Azadirachtin (Aza) used as insecticide due to inhibiting growth of insects and preventing them from feeding on plants. To understand the effects of contamination of this insecticide on phototrophs, and to determine the responses of these organisms against these insecticides are extremely important in understanding how the ecosystem is affected. In this study, chlorophyll-a amount, OD 560 and antioxidant parameters (total SOD, APX, GR, Proline, MDA and H2O2) were determined in order to understand the effect of Aza on Arthrospira platensis Gomont. Aza was applied between 0–20 μg mL−1 concentrations for 7 days in the study. Enzyme analysis was conducted at the end of the 7th day. There was a statistically significant decrease in the absorbance of OD560 and the chlorophyll-a content in A. platensis cultures exposed to the Aza (0–20 μg mL−1) during 7 days due to the increase in pesticide levels. SOD activity decreased at 8, 16 and 20 μg mL−1 concentrations; GR enzyme activity showed a significant decrease compared to the control at a concentration of 20 μg mL−1. APX activity did not change significantly compared to control. The MDA content increased significantly at 16 and 20 μg mL−1 concentrations. The H2O2 content significantly increased at 12, 16 and 20 μg mL−1 concentrations (p < 0.05) while the free proline content decreased at 4 μg mL−1 concentration (p < 0.05). As a result, regarding the Aza concentrations used in this study may be a step to prevent pesticide pollution in the environment.

scholarly journals THE CORRELATION BETWEEN FAT INTAKE AND PLASMA SUPEROXIDE DISMUTASE ACTIVITY WITH TELOMERE LENGTH OF MINANGKABAU ETHNICITY MEN, IN WEST SUMATERA, INDONESIA

2019 ◽  
pp. 305-309
Author(s):  
AFRIWARDI AFRIWARDI ◽  
DELMI SULASTRI ◽  
YUNIAR LESTARI ◽  
DESMAWATI DESMAWATI ◽  
PRIMA MINERVA
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Fatty Acid ◽  
Telomere Length ◽  
Saturated Fatty Acids ◽  
Cross Sectional Study ◽  
Fat Intake ◽  
Sod Activity ◽  
Cross Sectional ◽  
Total Fat

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia. Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity. Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037). Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.

Switching enzyme activity by a temperature responsive inhibitor modified polymer

2020 ◽  
Vol 56 (16) ◽  
pp. 2459-2462 ◽  
Author(s):  
Falko O. Rottke ◽  
Marie-Victoria Heyne ◽  
Stefan Reinicke
Keyword(s):  
Enzyme Activity ◽  
Acetylcholinesterase Inhibitor ◽  
Temperature Responsive ◽  
Modified Polymer

A thermoresponsive NIPAAm-based polymer is combined with the selective acetylcholinesterase inhibitor tacrine in order to create a strict in sense on/off switch for enzyme activity.

scholarly journals Effect of Pulsed Ultraviolet Light on Natural Microbial Load and Antioxidant Properties of Fresh Blueberries

2018 ◽  
Vol 6 (6) ◽  
pp. 733
Author(s):  
Senem Guner ◽  
Zeynal Topalcengiz
Keyword(s):  
Enzyme Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Properties ◽  
Antioxidant Enzyme Activity ◽  
Microbial Load ◽  
Plate Count ◽  
Light Illumination ◽  
Sod Activity ◽  
Aerobic Plate Count

Blueberries have been drawn more attention in recent years for their antioxidant capacities and proposed health benefits. Pulsed ultraviolet (PUV) light is known for its disinfection effects on the surface of fresh fruits. This study’s aim was to examine the effects of PUV light on naturally present microbial load, antioxidant capacity, and antioxidant enzyme activity of fresh blueberries. Fresh blueberry samples were treated for 30, 60, 90, and 120 s. Samples were stabilized at 13 cm away from the PUV lamp before treatment. Total Aerobic Plate Count (TAPC) and yeast and mold count reduction were observed the highest in samples treated for 90 s, 1.97 and 1.27 log CFU/g, respectively. Antioxidant capacity levels were significantly different for treated samples for 60 and 90 s in FRAP analysis, whereas no differences were observed in results of DPPH analysis. Superoxide dismutase (SOD) activity as antioxidant enzyme had no difference, however, glutathione peroxidase (GPx) increased by 50% with PUV treatment for 120 s. PUV light treatment of fresh blueberries for 90 s was determined as ideal exposure time among tested time intervals of this study. PUV light illumination can generally enhance antioxidant capacity and antioxidant enzyme activity of fresh blueberries and decrease the population of natural microorganisms of fresh blueberries and similar small fruits.

scholarly journals Effect of Swiftlet’s (Collocalia Fuciphago) Nest Extract on the Malondialdehyde (MDA) and Superoxyde Dismutase (SOD) Activity on Hyperglycemic Rattus Norvegicius

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Francisca Diana Alexandra ◽  
Agnes Frethernety ◽  
Elsa Trinovita ◽  
Triawanti Triawanti
Keyword(s):  
Enzyme Activity ◽  
Blood Glucose ◽  
Blood Serum ◽  
Water Extract ◽  
Control Group ◽  
Sod Activity ◽  
Control Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Elevated Glucose

Hyperglycemia that occurs in diabetic Mellitus leads to glycation reactions in protein molecules and oxidative stress resulting in damage to cells and organs. Swiftlet’s nest believed society could lower blood glucose. The objective of the study was to evaluate the effect of Swiftlet’s nest (Collocalia fuciphago) extract on glucose level, Malondialdehyde (MDA) levels, and Superoxide Dismutase (SOD) activity in blood serum. The study used Posttest-Only with Control Group Design, consisting of 1 control group (given aqua dest) and 3 treatment groups (dose 1; 10 and 100 mg/kg BW). Each group consisted of 6 Rattus norvegicius. Before being treated, Streptozotocin-induced rat at a dose of 68 mg/kg BW intraperitoneal. On the 7th day after induction, rats had elevated glucose ± 102 - 108 mg/dL. Then the rats were given water extract Swiftlet’s nest for 28 days orally. All data were analyzed by the Kruskal-Wallis test, followed by the Mann-Whitney test, with a 95% confidence level. The results of blood glucose levels in each group (K, P1, P2 and P3) were 111.0 vs 88.5 vs 86 vs 83 mg / dL (p = 0.035), MDA levels experienced an increase in the treatment group compared to controls namely 193.50 vs 193.83 vs. 198.50 nmol / mL, p = 0.001. While the SOD enzyme activity has increased, namely 0.0050 vs. 0.0075 vs. 0.0263%. In conclusion, Swiftlet’s nest water extract can reduce blood glucose levels and increase MDA levels and SOD enzyme activity in blood serum. 

scholarly journals Growth, Secondary Metabolites and Enzyme Activity Responses of Two Edible Fern Species to Drought Stress and Rehydration in Northeast China

Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 137 ◽  
Author(s):  
Yanlin Wang ◽  
Shanshan Gao ◽  
Xingyuan He ◽  
Yan Li ◽  
Peiyang Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Drought Stress ◽  
Secondary Metabolites ◽  
Drought Resistance ◽  
Northeast China ◽  
Resistance Mechanism ◽  
Leaf Water Content ◽  
Total Phenols ◽  
Sod Activity ◽  
Fern Species

The drought resistance mechanism of Matteuccia struthiopteris (L.) Todar. and Athyrium multidentatum (Doll.) Ching were measured under natural drought exposure. The results showed that the two edible fern species showed stronger resistance in the early stages of drought, mainly expressed as the decrease of relative leaf water content (RLWC), increase of osmotic substances, secondary metabolites such as flavonoids (FC), total phenols (TPC), proantho cyanidins (PCC) content and enzyme activity (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX)). The higher RLWC, FC, TPC, PCC and abscisic acid (ABA) content and lower H2O2 content indicates the stronger non-enzymatic antioxidant system and drought resistance of A. multidentatum. However, the proline (Pro) content changed slowly, and the synthesis of soluble protein (SP), total phenols, proantho cyanidins and ABA, SOD activity of two fern species were inhibited in the late stages of drought stress. This study can provide a scientific basis for the cultivation and utilization of edible fern species under forest in Northeast China.

Effects of Nonylphenol on the Enzyme Activity of SOD in Shrimp (Neocaridina heteropoda)

2014 ◽  
Vol 1073-1076 ◽  
pp. 256-258
Author(s):  
Hong Wei Wang ◽  
Qing Chao Xing ◽  
Jie Zhang ◽  
Rui Yin ◽  
Duan Bo Cai ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Sod Activity ◽  
Physiological Indicators ◽  
Relationship Of ◽  
The Relationship

The effects of different concentrations of nonylphenol on Neocaridina heteropoda were studied through testing the Superoxide Dismutase (SOD) activity in the muscle of N. heteropoda under the nonylphenol stress, then explored the relationship of the time that was N. heteropoda exposed nonylphenol and the in vivo physiological indicators. Results showed the following conclusion: in a certain range, higher concentrations of nonylphenol had heavier influence on SOD activity of shrimps; shrimps were infected significantly when they were exposed to the same concentration of nonylphenol in 2 days; the influence was reduced to the shrimp with the prolonging of time.

scholarly journals   Antioxidative mechanisms on selenium accumulation in Pteris vittata L., a potential selenium phytoremediation plant

2012 ◽  
Vol 58 (No. 3) ◽  
pp. 105-110 ◽  
Author(s):  
R.W. Feng ◽  
C.Y. Wei
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Pteris Vittata ◽  
Sod Activity ◽  
Antioxidant Responses ◽  
Industrial Activities ◽  
Chinese Brake Fern ◽  
Selenium Accumulation ◽  
Pteris Vittata L

Selenium (Se) contamination due to industrial activities has received increasing concerns. Phytoremediation has been suggested to be an efficient and feasible way to remove Se from Se-contaminated environment. Recently, an arsenic (As) hyperaccumulator Pteris vittata L. (Chinese Brake fern) was found to be a Se accumulator. This study was carried out to investigate Se accumulation mechanisms concentrating on antioxidant responses of this plant to six levels of selenite (0, 1, 2, 5, 10, and 20 mg/L). The results showed that Chinese Brake fern can accumulate a large amount of Se without any visible toxic symptoms and significant decreases in its biomass. However, the root took up more Se than the fronds. The highest concentration of Se in the roots and fronds was 1.536 mg/kg and 242 mg/kg, respectively, demonstrating a typical accumulation character to Se. Addition of 2 mg/L Se decreased, but &ge; 5&nbsp;mg/L Se enhanced the production of malondialdehyde (MDA), suggesting an antioxidant role of low dosages of Se. The enzymes of catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD) contributed their anti-oxidative functions only under low dosages of Se, as shown by their increased activities at Se levels &le; 5 mg/L and lowered activities at Se levels &gt; 5 mg/L. The concentration of glutathione (GSH) and enzyme activity of glutathione reductase (GR) were stimulated by &ge; 5 mg/L Se. Superoxide dismutase (SOD) activity was also enhanced by 20 mg/L Se. Our results suggest that SOD, GSH and GR were likely responsible for, but enzymes of POD, APX, and CAT have limited roles in Se accumulation in Chinese Brake fern. &nbsp;

scholarly journals THE CORRELATION BETWEEN FAT INTAKE AND PLASMA SUPEROXIDE DISMUTASE ACTIVITY WITH TELOMERE LENGTH OF MINANGKABAU ETHNICITY MEN, IN WEST SUMATERA, INDONESIA

2019 ◽  
Author(s):  
Afriwadi ◽  
Delmi Sulastri ◽  
Yuniar Lestari ◽  
Prima Minerva
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Fatty Acid ◽  
Telomere Length ◽  
Saturated Fatty Acids ◽  
Cross Sectional Study ◽  
Fat Intake ◽  
Sod Activity ◽  
Cross Sectional ◽  
Total Fat

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia.Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity.Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p&gt;0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037).Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.

scholarly journals The influence of fullerenol on antioxidative enzyme activity in irradiated human erythroleukemic cell line (K562)

2007 ◽  
Vol 61 (3) ◽  
pp. 164-166
Author(s):  
Visnja Bogdanovic ◽  
Karmen Stankov ◽  
Aleksandra Nikolic ◽  
Ivana Icevic ◽  
Slavica Solajic ◽  
...  
Keyword(s):  
Cell Culture ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
X Rays ◽  
Control Groups ◽  
Sod Activity ◽  
Applied Dose ◽  
Erythroleukemic Cell ◽  
Erythroleukemic Cell Line ◽  
Total Superoxide Dismutase

Cell culture K562 samples were treated with fullerenol (C6o(OH)24) at a concentration of 10 nmol/mL and thereafter irradiated with X-rays (24Gy). The activity of gamma-glutamyltransfrease (?-GT), total superoxide-dismutase (SOD) and glutathion-peroxidase (GSH-Px) was determined 1, 24 and 48 hours after irradiation. Irradiation induces an increase in the activity of all the investigated enzymes. Fullerenol in the applied dose decreased the ?-GT activity 24 and 48 h after irradiation. The total SOD activity is increased in both pretreated groups except in the iradiated group at the 48th hour. Treatment with fullerenol before irradiation increased GSH-Px activity in irradiated groups and decreased it in the control groups.

Sign in / Sign up

Export Citation Format

Share Document