Exploration of Antibacterial and Wound Healing Potential of PLGA/silk fibroin Based Electrospun Membrane Loaded with Zinc oxide Nanoparticles

2021 ◽  
Author(s):  
Atta ur Rehman Khan ◽  
Kai Huang ◽  
Jinzhong Zhao ◽  
Tonghe Zhu ◽  
Yos Morsi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Cell Migration ◽  
Silk Fibroin ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Electrospun Membrane ◽  
Stimulate Cell ◽  
Anti Inflammatory

Zinc oxide nanoparticles (ZnO NP) are known for their antibacterial, antioxidant, and anti-inflammatory activities. Moreover, ZnO NP can stimulate cell migration, re-epithelialization, and angiogenesis. All these attributes are highly relevant...

scholarly journals Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 713
Author(s):  
Nina Melnikova ◽  
Alexander Knyazev ◽  
Viktor Nikolskiy ◽  
Peter Peretyagin ◽  
Kseniia Belyaeva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Bacterial Cellulose ◽  
Zinc Oxide Nanoparticles ◽  
Wound Dressings ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Wound Model ◽  
And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

scholarly journals Plant Mediated Green Synthesis of Zinc Oxide Nanoparticles for Burn Wound Therapy

2020 ◽  
Author(s):  
Mohd Riyaz Beg ◽  
Shital Ghodinde ◽  
Vidhi Gupta
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Zinc Oxide ◽  
Green Synthesis ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Burn Wounds ◽  
Burn Wound Healing

In this changing world, we all are surrounded by the surmountable risk of getting injured. Amongst various risk factors, major burns are the most distressing and catastrophic. Burn wounds are not easy to heal via natural healing process and ultimately ended up with scar formation. If the degree of burn is high then the loss of tissue and its function is very common. To fasten-up the natural burn wound healing; zinc, an essential trace element is found to be very much effective. But due to its&rsquo; particle size limitation, less contact with wounded cells and tissues, and high inherent toxicity restrict its use. Needlessly, zinc is an element with dual action i.e. both antimicrobial and wound healing it is a prime choice to apply its aptitude in burn wound healing. To overcome the documented limitations zinc has converted to nanoparticle form. Zinc oxide nanoparticles, in particular, have attained ample of interest due to their unique properties and potential antimicrobial activity along with wound healing activity which makes it promising for the healing of topical burn wounds. Plant mediated green synthesis of nano-metal oxide particles is gaining a lot of significance due to its simplicity, eco-friendliness and extensive antimicrobial activity and recommended as an appealing substitute to not only physical methods but also chemical methods avoiding the use of the high rate of toxic chemicals and extreme surroundings. This study includes ZnO NPs role in burn wound healing with Phyto-mediated synthesis methods to provide evidence of their potential applications. Additionally, it provides an overview of traditional methods used for the synthesis of ZnO nanoparticles and characterization techniques to obtain information concerning the size, shape and optical properties along with toxicity and safety concern of ZnO NPs and its biomedical applications.

scholarly journals Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4896
Author(s):  
Siva Sankar Sana ◽  
Divya Vishambhar Kumbhakar ◽  
Akbar Pasha ◽  
Smita C. Pawar ◽  
Andrews Nirmala Grace ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Cell Migration ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Positive Control ◽  
Ftir Spectra ◽  
Total Phenolic ◽  
Oxide Nanoparticles ◽  
Zno Nps

In this work, we present an ecofriendly, non-hazardous, green synthesis of zinc oxide nanoparticles (ZnO NPs) by leaf extract of Crotalaria verrucosa (C. verrucosa). Total phenolic content, total flavonoid and total protein contents of C. verrucosa were determined. Further, synthesized ZnO NPs was characterized by UV–visible spectroscopy (UV-vis), X-ray diffractometer (XRD), Fourier transform infra-red (FTIR) Spectra, transmission electron microscope (TEM), and Dynamic light scattering (DLS) analysis. UV-vis shows peak at 375 nm which is unique to ZnO NPs. XRD analysis demonstrates the hexagonal phase structures of ZnO NPs. FTIR spectra demonstrates the molecules and bondings associated with the synthesized ZnO NPs and assures the role of phytochemical compounds of C. verrucosa in reduction and capping of ZnO NPs. TEM image exhibits that the prepared ZnO NPs is hexagonal shaped and in size ranged between 16 to 38 nm which is confirmed by DLS. Thermo-gravimetric analysis (TGA) was performed to determine the thermal stability of biosynthesized nanoparticles during calcination. The prepared ZnO NPs showed significant antibacterial potentiality against Gram-positive (S. aureus) and Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, and Escherichia coli) pathogenic bacteria and SEM image shows the generalized mechanism of action in bacterial cell after NPs internalization. In addition, NPs are also found to be effective against the studied cancer cell lines for which cytotoxicity was assessed using MTT assay and results demonstrate highest growth of inhibition at the concentration of 100 µg/mL with IC50 value at 7.07 µg/mL for HeLa and 6.30 µg/mL for DU145 cell lines, in contrast to positive control (C. verrucosa leaf extract) with IC50 of 22.30 µg/mL on HeLa cells and 15.72 µg/mL on DU145 cells. Also, DAPI staining was performed in order to determine the effect on nuclear material due to ZnO NPs treatment in the studied cell lines taking leaf extract as positive control and untreated negative control for comparison. Cell migration assay was evaluated to determine the direct influence of NPs on metastasis that is potential suppression capacity of NPs to tumor cell migration. Outcome of the synthesized ZnO NPs using C. verrucosa shows antimicrobial activity against studied microbes, also cytotoxicity, apoptotic mediated DNA damage and antiproliferative potentiality in the studied carcinoma cells and hence, can be further used in biomedical, pharmaceutical and food processing industries as an effective antimicrobial and anti-cancerous agent.

scholarly journals Antibacterial Ferroelectric Hybrid Membranes Fabricated via Electrospinning for Wound Healing

Membranes ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 986
Author(s):  
Ivan V. Lukiev ◽  
Ludmila S. Antipina ◽  
Semen I. Goreninskii ◽  
Tamara S. Tverdokhlebova ◽  
Dmitry V. Vasilchenko ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Vinylidene Fluoride ◽  
Antibacterial Properties ◽  
Oxide Nanoparticles ◽  
Hybrid Membranes ◽  
Electrospinning Technique ◽  
Β Phase

In the present study, wound healing ferroelectric membranes doped with zinc oxide nanoparticles were fabricated from vinylidene fluoride-tetrafluoroethylene copolymer and polyvinylpyrrolidone using the electrospinning technique. Five different ratios of vinylidene fluoride-tetrafluoroethylene to polyvinylpyrrolidone were used to control the properties of the membranes at a constant zinc oxide nanoparticle content. It was found that an increase of polyvinylpyrrolidone content leads to a decrease of the spinning solution conductivity and viscosity, causing a decrease of the average fiber diameter and reducing their strength and elongation. By means of X-ray diffraction and infrared spectroscopy, it was revealed that increased polyvinylpyrrolidone content leads to difficulty in crystallization of the vinylidene fluoride-tetrafluoroethylene copolymer in the ferroelectric β-phase in membranes. Changing the ratio of vinylidene fluoride-tetrafluoroethylene copolymer and polyvinylpyrrolidone with a constant content of zinc oxide nanoparticles is an effective approach to control the antibacterial properties of membranes towards Staphylococcus aureus. After carrying out in vivo experiments, we found that ferroelectric hybrid membranes, containing from five to ten mass percent of PVP, have the greatest wound-healing effect for the healing of purulent wounds.

scholarly journals BIOFABRICATION OF ZINC OXIDE NANOPARTICLES USING PTEROCARPUS MARSUPIUM AND ITS BIOMEDICAL APPLICATIONS

2019 ◽  
Vol 12 (1) ◽  
pp. 245
Author(s):  
Shymala Rajan Abhinaya ◽  
Ramakrishnan Padmini
Keyword(s):  
Lipid Peroxidation ◽  
Zinc Oxide ◽  
Ftir Spectroscopy ◽  
Zinc Acetate ◽  
Zinc Oxide Nanoparticles ◽  
Biomedical Applications ◽  
Oxide Nanoparticles ◽  
Pterocarpus Marsupium ◽  
Uv Visible ◽  
Anti Inflammatory

Objective: The objective of the study is to perform the synthesis of zinc oxide nanoparticles using the bark extract of Pterocarpus marsupium and to evaluate its biomedical applications.Methods: Various concentrations of zinc acetate are used, and synthesis conditions were optimized to get a stable nanoparticle. The finest synthesis condition for zinc oxide nanoparticle production was at pH 7 with 20 ml extract, zinc acetate 10 mM, and 120 min of reaction time. The synthesized nanopowder was characterized using various analytical techniques, such as ultraviolet (UV)-visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The synthesized nanoparticles were tested for their antimicrobial, anti-inflammatory, inhibition of lipid peroxidation, and inhibition of amylase activity.Results: The size range of nanoparticles obtained was in the range of 10–32 nm as reported by SEM. The UV-visible absorption spectrum of the synthesized nanoparticle showed a peak at 340 nm, which confirmed the presence of nanoparticles. FTIR spectroscopy analysis indicated the presence of zinc oxide stretching at 666.22 cm-1. Further, the IR spectra indicated the presence of alcohols and acids, which can act as capping agents around the nanoparticles. XRD analysis confirmed the crystalline nature of nanoparticles.The synthesized nanoparticle showed appreciable antimicrobial activity. Zinc oxide nanoparticles at 40 μg/well were tested against phytopathogens, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus flavus, and Aspergillus niger showed 16, 13, 15, and 16 mm zones of inhibition, respectively. The synthesized nanoparticle showed a considerable increase in inhibition of lipid peroxidation and amylase activity. The nanoparticle also exhibited appreciable anti-inflammatory activity measured by the inhibition of albumin denaturation.Conclusion: The study instigates the simple and convenient method of synthesizing zinc oxide nanoparticles using P. marsupium and its few biomedical applications.

scholarly journals Antimicrobial PAA/PAH Electrospun Fiber Containing Green Synthesized Zinc Oxide Nanoparticles for Wound Healing

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2889
Author(s):  
Marina Bandeira ◽  
Bor Shin Chee ◽  
Rafaele Frassini ◽  
Michael Nugent ◽  
Marcelo Giovanela ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Electrospun Fiber ◽  
Ilex Paraguariensis ◽  
Resistant Bacteria ◽  
Oxide Nanoparticles ◽  
Sem Images ◽  
Electrospinning Technique

Wound infections are the main complication when treating skin wounds. This work reports a novel antimicrobial material using green synthesized zinc oxide nanoparticles (ZnONPs) incorporated in polymeric fibers for wound healing purposes. ZnONPs are a promising antimicrobial nanomaterial with high activity against a range of microorganisms, including drug-resistant bacteria. The electrospun fibers were obtained using polyacrylic acid (PAA) and polyallylamine hydrochloride (PAH) and were loaded with ZnONPs green synthesized from Ilex paraguariensis leaves with a spherical shape and ~18 nm diameter size. The fibers were produced using the electrospinning technique and SEM images showed a uniform morphology with a diameter of ~230 nm. EDS analysis proved a consistent dispersion of Zn in the fiber mat, however, particle agglomerates with varying sizes were observed. FTIR spectra confirmed the interaction of PAA carboxylic groups with the amine of PAH molecules. Although ZnONPs presented higher antimicrobial activity against S. aureus than E. coli, resazurin viability assay revealed that the PAA/PAH/ZnONPs composite successfully inhibited both bacteria strains growth. Photomicrographs support these results where bacteria clusters were observed only in the control samples. The PAA/PAH/ZnONPs composite developed presents antimicrobial activity and mimics the extracellular matrix morphology of skin tissue, showing potential for wound healing treatments.

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