Basis of coffee seed sensitivity to liquid nitrogen exposure: oxidative stress or imbibitional damage?

2003 ◽  
Vol 119 (4) ◽  
pp. 534-543 ◽  
Author(s):  
Stephane Dussert ◽  
Nathalie Chabrillange ◽  
Jean-Luc Montillet ◽  
Jean-Pierre Agnel ◽  
Florent Engelmann ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liquid Nitrogen ◽  
Imbibitional Damage ◽  
Coffee Seed

scholarly journals Thymoquinone Improves Motility, Plasma Membrane and DNA Integrity of Frozen‐Thawed Ram Semen

2021 ◽  
Author(s):  
Muhammed Enes Inanc ◽  
Şükrü Güngör ◽  
Fatih Avdatek ◽  
Deniz Yeni ◽  
Mehmet Fuat Gülhan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Plasma Membrane ◽  
Liquid Nitrogen ◽  
Nigella Sativa ◽  
Stress Index ◽  
Dna Integrity ◽  
Protective Effects ◽  
Oxidative Stress Index ◽  
Biochemical Alterations

Abstract This study aimed to determine the protective effects of Thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of frozen‐thawed ram semen. Ejaculates were collected from five Sonmez rams. Ejaculates were extended with control (0 µg/mL TQ), 10, 25, 50 and 100 µg/mL TQ supplemented Tris-based extender. Then extended semen samples were equilibrated in a +4 °C cold cabinet for 2 h. After 2 h the samples loaded into 0.25 mL French straws. The straws were then frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (-196 °C). For analyses, frozen samples were thawed in a water bath (37 oC for 30 s) and evaluated in terms of motility characteristics, plasma membrane acrosome integrity (PMAI), mitochondrial reactive oxygen spices level (MITOSOX+), lipid peroxidation level (BODIPY+), DNA damage and biochemical alterations (oxidative stress index, malondialdehide and glutathione). TQ 100 had higher total and progressive motility compared to control (P˂0.05). According to motility characteristics there were significant differences between the groups on curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; P˂0.05). The highest DNA damage was detected in control groups (P˂0.05). TQ50 had higher PMAI compared to control and TQ25 (P˂0.05). The lowest MITOSOX+ level was detected in TQ50 and TQ100 groups (P˂0.05). There were no significant differences on BODIPY+, oxidative stress index, malondialdehide and glutathione (P>0.05). According to results, it could be concluded that, supplementing 50 and 100µg/mL TQ to Tris extender that was used for ram semen cryopreservation, showed a positive effect on motility, PMAI and also decreased DNA damage and MITOSOX+.

scholarly journals Effects of mucuna seed extract on sperm functional integrities and seminal oxidative stress indices of vitrified goat semen

2017 ◽  
Vol 62 (2) ◽  
pp. 155-166
Author(s):  
Daramola Olamitibo ◽  
Oyewusi Adeoye ◽  
Sorongbe Amidu ◽  
Adekunle Oluwafemi ◽  
Iyanda Ayobami ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liquid Nitrogen ◽  
Seed Extract ◽  
West African ◽  
Stress Indices ◽  
West African Dwarf ◽  
Optimal Values ◽  
Acrosin Activity

Effects of mucuna seed extract (MSE) in tris-extenders on sperm functional integrities and seminal oxidative stress indices of vitrified sperm of West African Dwarf (WAD) goat bucks were investigated. Varying levels (0, 0.25, 0.5, 0.75, 1 g/100 mL of diluent) of MSE in tris-extenders were diluted with semen specimens. Diluted semen specimens were vitrified and preserved in liquid nitrogen. The results showed that sperm functional integrities (acrosome and membrane integrities) in MSE extenders were comparable. However, seminal oxidative stress indices (malondialdehyde [MDA] and acrosin activity) differed (P<0.05) among the various treatments and the control. The results revealed that semen vitrified with MSE had reduced (P<0.05) MDA concentrations compared to the control. Higher (P<0.05) acrosin activities were recorded in semen vitrified with MSE compared to the control except at 0.25g MSE and optimal values were recorded at 1g MSE. The findings indicated that MSE in the tris-extenders reduced MDA concentration with congruent elevated acrosin activity and consequently maintained sperm functional integrities of vitrified spermatozoa of the WAD goat.

Electron Probe Microanalysis of Frozen Hydrated Kidneys, Isolated Cells, and Cultured Cells

1982 ◽  
Vol 40 ◽  
pp. 402-403 ◽  
Author(s):  
Claude Lechene
Keyword(s):  
Liquid Nitrogen ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Cultured Cells ◽  
Liquid Nitrogen Temperature ◽  
Elemental Content ◽  
Isolated Cells ◽  
Renal Tubular Cells ◽  
Diamond Saw ◽  
Saw Blade

Electron probe microanalysis of frozen hydrated kidneysThe goal of the method is to measure on the same preparation the chemical elemental content of the renal luminal tubular fluid and of the surrounding renal tubular cells. The following method has been developed. Rat kidneys are quenched in solid nitrogen. They are trimmed under liquid nitrogen and mounted in a copper holder using a conductive medium. Under liquid nitrogen, a flat surface is exposed by sawing with a diamond saw blade at constant speed and constant pressure using a custom-built cryosaw. Transfer into the electron probe column (Cameca, MBX) is made using a simple transfer device maintaining the sample under liquid nitrogen in an interlock chamber mounted on the electron probe column. After the liquid nitrogen is evaporated by creating a vacuum, the sample is pushed into the special stage of the instrument. The sample is maintained at close to liquid nitrogen temperature by circulation of liquid nitrogen in the special stage.

A Liquid Nitrogen Cooled Stage for STEM

1974 ◽  
Vol 32 ◽  
pp. 444-445
Author(s):  
Louis T. Germinario
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Liquid Nitrogen ◽  
Room Temperature ◽  
Objective Lens ◽  
Thermal Drift ◽  
Specimen Holder ◽  
Resolution Capability ◽  
Focal Position

A liquid nitrogen stage has been developed for the JEOL JEM-100B electron microscope equipped with a scanning attachment. The design is a modification of the standard JEM-100B SEM specimen holder with specimen cooling to any temperatures In the range ~ 55°K to room temperature. Since the specimen plane is maintained at the ‘high resolution’ focal position of the objective lens and ‘bumping’ and thermal drift la minimized by supercooling the liquid nitrogen, the high resolution capability of the microscope is maintained (Fig.4).

Field-Ion Microscopy of BCC Metals: A Study of Image Differences and Topographical Variations

1973 ◽  
Vol 31 ◽  
pp. 114-115
Author(s):  
O. T. Inal ◽  
L. E. Murr
Keyword(s):  
Liquid Nitrogen ◽  
Liquid Nitrogen Temperature ◽  
Surface Atom ◽  
Image Brightness ◽  
Field Ion Microscopy ◽  
Topographic Features ◽  
Bcc Metals ◽  
Electron Orbital ◽  
Ion Microscopy ◽  
Field Ion Microscope

When sharp metal filaments of W, Fe, Nb or Ta are observed in the field-ion microscope (FIM), their appearance is differentiated primarily by variations in regional brightness. This regional brightness, particularly prominent at liquid nitrogen temperature has been attributed in the main to chemical specificity which manifests itself in a paricular array of surface-atom electron-orbital configurations.Recently, anomalous image brightness and streaks in both fcc and bee materials observed in the FIM have been shown to be the result of surface asperities and related topographic features which arise by the unsystematic etching of the emission-tip end forms.

The Effects of Drying Techniques on Clay-Rich Soil Texture

1974 ◽  
Vol 32 ◽  
pp. 466-467
Author(s):  
T. G. Naymik
Keyword(s):  
Critical Point ◽  
Liquid Nitrogen ◽  
Liquid Nitrogen Temperature ◽  
Drying Methods ◽  
Air Drying ◽  
Freeze Dried ◽  
Critical Point Drying ◽  
Set Up ◽  
The Relationship ◽  
Quartz Grains

Three techniques were incorporated for drying clay-rich specimens: air-drying, freeze-drying and critical point drying. In air-drying, the specimens were set out for several days to dry or were placed in an oven (80°F) for several hours. The freeze-dried specimens were frozen by immersion in liquid nitrogen or in isopentane at near liquid nitrogen temperature and then were immediately placed in the freeze-dry vacuum chamber. The critical point specimens were molded in agar immediately after sampling. When the agar had set up the dehydration series, water-alcohol-amyl acetate-CO2 was carried out. The objectives were to compare the fabric plasmas (clays and precipitates), fabricskeletons (quartz grains) and the relationship between them for each drying technique. The three drying methods are not only applicable to the study of treated soils, but can be incorporated into all SEM clay soil studies.

Si(Li) detector for microanalysis cooled by thermoelectric device

1992 ◽  
Vol 50 (2) ◽  
pp. 1736-1737
Author(s):  
Shaul Barkan
Keyword(s):  
Solid State ◽  
Liquid Nitrogen ◽  
Thermoelectric Device ◽  
Peltier Cooler ◽  
Complicated Process ◽  
The Common

Cooling down solid state detecors, with other different way then liquid Nitrogen, is a goal of many vendors and customers since the invention of these detectors. THe disadvantage of the common way of liquid Nitrogen is first the inavailibility of the LN in many uses (like space military and any other applications that are not done inside a well organize Laboratory). The use of LN also considers as a Labor consumer in addition to the big dewar that has to be added to any detector for storing the LN, the boiling of the LN, may cause microphonics problesm and the refiling of the dewar in many Labs is a complicated process due to inconvenience location of the microscope.In this paper I will show a spectra result of 10mm2 SiLi detector for microanalysis use, cooled by peltier cooler. The peltier cooler has the advantage of non-microphonics and non-labor needed (like adding LN to the dewar).

Water accumulation as a function of temperature on specimens in an electron microscope cold stage

1986 ◽  
Vol 44 ◽  
pp. 114-115 ◽  
Author(s):  
M.K. Lamvik ◽  
D.A. Kopf ◽  
S.D. Davilla ◽  
J.D. Robertson
Keyword(s):  
Electron Microscope ◽  
Mass Loss ◽  
Liquid Helium ◽  
Liquid Nitrogen ◽  
Liquid Nitrogen Temperature ◽  
Liquid Helium Temperature ◽  
Helium Temperature ◽  
Cold Stage ◽  
Water Accumulation ◽  
Better Than

Last year we reported1 that there is a striking reduction in the rate of mass loss when a specimen is observed at liquid helium temperature. It is important to determine whether liquid helium temperature is significantly better than liquid nitrogen temperature. This requires a good understanding of mass loss effects in cold stages around 100K.

A conduction-cooled stage for high-resolution Cryo-SEM

1992 ◽  
Vol 50 (2) ◽  
pp. 1282-1283
Author(s):  
John G. Sheehan
Keyword(s):  
High Resolution ◽  
Liquid Nitrogen ◽  
Mechanical Stability ◽  
Cooling System ◽  
Cold Trap ◽  
Nitrogen Gas ◽  
Particulate Suspensions ◽  
Advantages And Disadvantages ◽  
Convection Cooling ◽  
Styrene Butadiene

The goal is to examine with high resolution cryo-SEM aqueous particulate suspensions used in coatings for printable paper. A metal-coating chamber for cryo-preparation of such suspensions was described previously. Here, a new conduction-cooling system for the stage and cold-trap in an SEM specimen chamber is described. Its advantages and disadvantages are compared to a convection-cooling system made by Hexland (model CT1000A) and its mechanical stability is demonstrated by examining a sample of styrene-butadiene latex.In recent high resolution cryo-SEM, some stages are cooled by conduction, others by convection. In the latter, heat is convected from the specimen stage by cold nitrogen gas from a liquid-nitrogen cooled evaporative heat exchanger. The advantage is the fast cooling: the Hexland CT1000A cools the stage from ambient temperature to 88 K in about 20 min. However it consumes huge amounts of liquid-nitrogen and nitrogen gas: about 1 ℓ/h of liquid-nitrogen and 400 gm/h of nitrogen gas. Its liquid-nitrogen vessel must be re-filled at least every 40 min.

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

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