Grapevine adaptation to drought: New candidate genes for the genotype-dependent response

Grapevine requires irrigation supply for its cultivation, especially in the arid and semiarid geographic areas. As consequence of the severe climatic changes, water consumption is becoming more and more important as environmental and cost factor that needs to be reduced. Water deficiency can affect berry and wine quality depending on the extent of plant perceived stress, which is a cultivar specific trait. In a four-year project, we tested the physiological and molecular responses to water deficiency of two different table grape cultivars, Italia and Autumn Royal, and we highlighted that they differently adapted to drought stress conditions. Physiological analyses on field-growth plants showed cultivar-specific variations in photosynthetic carbon assimilation and, stomatal conductance under water deficiency. We further combined “omic” analyses to identify candidate genes involved in drought stress response and adaptative traits. Microarray analyses revealed a broad response of cultivar Italia to drought stress conditions characterized by the modulation of 1037 genes involved in biological processes as cell wall organization, carbohydrate metabolism, ROS response, response to hormone and osmotic stress. On the contrary, Autumn Royal response was limited to the modulation of only 29 genes mainly involved in plant stress response, nitrogen metabolism and hormone signal transduction. Our data highlighted that ABA-perception and –signalling are key factors mediating the varietal-specific behavior of the early response to drought.

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Differential Gene Expression Reveals Candidate Genes for Drought Stress Response in Abies alba (Pinaceae)

PLoS ONE ◽

10.1371/journal.pone.0124564 ◽

2015 ◽

Vol 10 (4) ◽

pp. e0124564 ◽

Cited By ~ 26

Author(s):

David Behringer ◽

Heike Zimmermann ◽

Birgit Ziegenhagen ◽

Sascha Liepelt

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Stress Response ◽

Candidate Genes ◽

Differential Gene Expression ◽

Abies Alba ◽

Differential Gene

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Pinpointing Genomic Regions Associated with Root System Architecture in Rice Through an Integrative Meta-Analysis Approach

10.21203/rs.3.rs-342601/v1 ◽

2021 ◽

Author(s):

Parisa Daryani ◽

Hadi Darzi Ramandi ◽

Sara Dezhsetan ◽

Raheleh Mirdar Mansuri ◽

Ghasem Hosseini Salekdeh ◽

...

Keyword(s):

Drought Stress ◽

Root System ◽

Candidate Genes ◽

System Architecture ◽

Meta Analysis ◽

Root System Architecture ◽

Stress Conditions ◽

Yield Potential ◽

Analysis Approach ◽

Genomic Regions

Abstract Root system architecture (RSA) is an important factor for facilitating water and nutrient uptake from deep soils and adaptation to drought stress conditions. In the present research, an integrated meta-analysis approach was employed to find candidate genes and genomic regions involved in rice RSA traits. A whole-genome meta-analysis was performed for 425 initial QTLs reported in 34 independent experiments controlling RSA traits under control and drought stress conditions in the previous twenty years. Sixty-four consensus meta-QTLs (MQTLs) were detected, unevenly distributed on twelve rice chromosomes. The confidence interval (CI) of the identified MQTLs was obtained as 0.11-14.23 cM with an average of 3.79 cM, which was 3.88 times narrower than the mean CI of the original QTLs. Interestingly, 52 MQTLs were co-located with SNP peak positions reported in rice genome-wide association studies (GWAS) for root morphological traits. The genes located in these RSA related MQTLs were detected, and explored to find the drought-responsive genes in the rice root based on the RNA-seq and microarray data. Multiple RSA and drought tolerance associated genes were found in the MQTLs including the genes involved in auxin biosynthesis or signaling (e.g. YUCCA, WOX, AUX/IAA, ARF), root angle (DRO1-related genes), lateral root development (e.g. DSR, WRKY), root diameter (e.g. OsNAC5), plant cell wall (e.g. EXPA) and lignification (e.g. C4H, PAL, PRX and CAD). The genes located both in the SNP peak positions and in the high-overview-index MQTLs for root architecture traits are suggested as novel candidate genes for further functional analysis.. The promising candidate genes and MQTLs would be applicable to genetic engineering and MQTL-assisted breeding of root phenotypes aimed at improving yield potential, stability and performance in a water-stressed environment.

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Glyoxalases and stress tolerance in plants

Biochemical Society Transactions ◽

10.1042/bst20130242 ◽

2014 ◽

Vol 42 (2) ◽

pp. 485-490 ◽

Cited By ~ 72

Author(s):

Charanpreet Kaur ◽

Ajit Ghosh ◽

Ashwani Pareek ◽

Sudhir K. Sopory ◽

Sneh L. Singla-Pareek

Keyword(s):

Stress Response ◽

Stress Tolerance ◽

Present Article ◽

Environmental Conditions ◽

Multigene Family ◽

Abiotic Stresses ◽

Plant Stress ◽

Stress Conditions ◽

Plant Stress Tolerance ◽

Adverse Environmental Conditions

The glyoxalase pathway is required for detoxification of cytotoxic metabolite MG (methylglyoxal) that would otherwise increase to lethal concentrations under adverse environmental conditions. Since its discovery 100 years ago, several roles have been assigned to glyoxalases, but, in plants, their involvement in stress response and tolerance is the most widely accepted role. The plant glyoxalases have emerged as multigene family and this expansion is considered to be important from the perspective of maintaining a robust defence machinery in these sessile species. Glyoxalases are known to be differentially regulated under stress conditions and their overexpression in plants confers tolerance to multiple abiotic stresses. In the present article, we review the importance of glyoxalases in plants, discussing possible roles with emphasis on involvement of the glyoxalase pathway in plant stress tolerance.

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Shared genetic architecture underlying root metaxylem phenotypes under drought stress in cereals

10.1101/2020.11.02.365247 ◽

2020 ◽

Author(s):

Stephanie P. Klein ◽

Jenna E. Reeger ◽

Shawn M. Kaeppler ◽

Kathleen M. Brown ◽

Jonathan P. Lynch

Keyword(s):

Water Stress ◽

Drought Stress ◽

Candidate Genes ◽

Stress Responses ◽

Genetic Architecture ◽

Association Studies ◽

Multiple Root ◽

Stress Conditions ◽

Genome Wide Association Studies ◽

New Strategy

AbstractRoot metaxylem are phenotypically diverse structures whose function is related to their anatomy, particularly under drought stress. Much research has dissected the genetic machinery underlying metaxylem phenotypes in dicots, but monocots are relatively unexplored. In maize (Zea mays), a robust pipeline integrated a GWAS of root metaxylem phenes under well-watered and water stress conditions with a gene co-expression network to identify candidate genes most likely to impact metaxylem phenotypes. We identified several promising candidate genes in 14 gene co-expression modules inferred to be functionally relevant to xylem development. We also identified five gene candidates that co-localized in multiple root metaxylem phenes in both well-watered and water stress conditions. Using a rice GWAS conducted in parallel, we detected overlapping genetic architecture influencing root metaxylem phenotypes by identifying eight pairs of syntenic candidate genes significantly associated with metaxylem phenes. There is evidence that the genes of these syntenic pairs may be involved in biosynthetic processes related to the cell wall, hormone signaling, oxidative stress responses, and drought responses. Our study demonstrates a powerful new strategy for identifying promising gene candidates and suggests several gene candidates that may enhance our understanding of vascular development and responses to drought in cereals.One sentence summaryCross-species genome-wide association studies and a gene coexpression network identified genes associated with root metaxylem phenotypes in maize under water stress and non-stress and rice.

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Molecular Characterisation of Soybean Osmotins and Their Involvement in Drought Stress Response

Frontiers in Genetics ◽

10.3389/fgene.2021.632685 ◽

2021 ◽

Vol 12 ◽

Author(s):

Giulia Ramos Faillace ◽

Paula Bacaicoa Caruso ◽

Luis Fernando Saraiva Macedo Timmers ◽

Débora Favero ◽

Frank Lino Guzman ◽

...

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Stress Response ◽

Stress Responses ◽

Developmental Stages ◽

Plant Stress ◽

3D Structure ◽

Plant Stress Responses ◽

Leaves And Roots ◽

Amino Acid Signature

Osmotins are multifunctional proteins belonging to the thaumatin-like family related to plant stress responses. To better understand the functions of soybean osmotins in drought stress response, the current study presents the characterisation of four previously described proteins and a novel putative soybean osmotin (GmOLPa-like). Gene and protein structure as well as gene expression analyses were conducted on different tissues and developmental stages of two soybean cultivars with varying dehydration sensitivities (BR16 and EMB48 are highly and slightly sensitive, respectively). The analysed osmotin sequences share the conserved amino acid signature and 3D structure of the thaumatin-like family. Some differences were observed in the conserved regions of protein sequences and in the electrostatic surface potential. P21-like present the most similar electrostatic potential to osmotins previously characterised as promoters of drought tolerance in Nicotiana tabacum and Solanum nigrum. Gene expression analysis indicated that soybean osmotins were differentially expressed in different organs (leaves and roots), developmental stages (R1 and V3), and cultivars in response to dehydration. In addition, under dehydration conditions, the highest level of gene expression was detected for GmOLPa-like and P21-like osmotins in the leaves and roots, respectively, of the less drought sensitive cultivar. Altogether, the results suggest an involvement of these genes in drought stress tolerance.

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