scholarly journals Correlation of the distribution of antioxidant enzyme concentrations in blood serum and heart tissue in rats

2020 ◽  
Vol 17 ◽  
pp. 00234
Author(s):  
Pavel Boriskin ◽  
Olga Gulenko ◽  
Anatoly Devyatkin ◽  
Rufia Karimova ◽  
Victor Leonov ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Lipid Peroxidation ◽  
Blood Serum ◽  
Free Radical Oxidation ◽  
Correlation Coefficients ◽  
Heart Tissue ◽  
Cellular Structures ◽  
Weak Correlation ◽  
Radical Oxidation ◽  
Disease Markers

Cardiovascular diseases have been the leading cause of death worldwide for many years. In recent years, new cardiovascular disease markers have been sought that can improve the diagnosis and treatment of this broad and prognostically unfavorable group of diseases. The efforts of many researchers are aimed at detecting changes in the level of enzymes in the lipid peroxidation system, which are antioxidants, as possible mechanisms underlying the development of cardiovascular disease. The imbalance between the intensification of free-radical oxidation caused by active oxygen forms and the activity of the body’s protective antioxidant system leads to serious disturbances: disorganization of cellular structures, changes in their functional activity. The article presents the study of the interrelation of the concentration distribution of enzymes of the lipid peroxidation system – antioxidants in blood serum and heart tissues of white non-pedigree rats. The correlation coefficients of Spearman, the gamma of correlation and Kendel Tau revealed a reliable presence of weak correlation between the concentration of glutathione peroxidase in blood serum and heart tissues (Spearman R = 0.18 at p ≤ 0.029408, Gamma = 0.14 at p ≤ 0.018701; Kendall Tau = 0.13 at p ≤ 0.018701).

scholarly journals Innovative approach to evaluating the correlation between the distribution of the concentration of enzymes in the blood serum and heart tissues of rats

2019 ◽  
Vol 135 ◽  
pp. 01097
Author(s):  
Pavel Boriskin ◽  
Olga Gulenko ◽  
Anatoliy Deviatkin ◽  
Victor Leonov ◽  
Olga Pavlova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Blood Serum ◽  
Concentration Distribution ◽  
Free Radical Oxidation ◽  
Correlation Coefficients ◽  
Active Oxygen ◽  
Cellular Structures ◽  
Weak Correlation ◽  
Radical Oxidation

Cardiovascular diseases (CVDs) have been the leading cause of death worldwide for many years. In recent years, new CVD markers have been sought that can improve the diagnosis and treatment of this broad and predictably unfavorable group of diseases. The efforts of many researchers are aimed at identifying changes in the level of enzymes in the lipid peroxidation system as possible mechanisms underlying the development of CVDs. The imbalance between the intensification of free-radical oxidation caused by active oxygen forms and the activity of the body’s protective antioxidant system leads to serious disturbances: disorganization of cellular structures, changes in their functional activity. The article presents the study of the interrelations between the concentration distribution of POL-AO enzymes in blood serum and heart tissues of white non-pedigree rats. The correlation coefficients of Spearman, the gamma of correlation and Kendel Tau revealed a reliable presence of weak correlation between the concentration of glutathione peroxidase in blood serum and heart tissues (Spearman R = 0.18 at p < 0.029408, Gamma = 0.14 at p < 0.018701; Kendall Tau = 0.13 at p < 0.018701).

scholarly journals Features of free radical lipid peroxidation and serum proteins at dental implants and peri-implantitis

2015 ◽  
Vol 96 (5) ◽  
pp. 756-759 ◽  
Author(s):  
D V Plyukhin ◽  
V E Tseylikman ◽  
O B Tseylikman ◽  
A I Sinitskiy
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Blood Serum ◽  
Dental Implants ◽  
Schiff Bases ◽  
Free Radical Oxidation ◽  
Protein Carbonylation ◽  
Radical Oxidation ◽  
Lipid Peroxidation Products ◽  
Dental Implantation

Aim. Determine the most informative indicators of free radical peroxidation in blood plasma for predicting the complications of dental implantation. Methods. The study included 84 patients of both with dental implants, including 31 patients without any complications and 53 patients with peri-implantitis. The levels of lipid peroxidation products and protein carbonylation were measured in peripheral blood. Results. Moderate intensification of lipid peroxidation and protein carbonylation was discovered in blood serum of patients with dental implants. Increased free radical oxidation in the blood was not limited to lipid peroxidation and resulted in increased serum levels of carbonylated proteins. Facilitation of oxidative degradation of proteins in the blood was associated with decreased resistance to «carbonyl stress», manifested as increased level of Fe+2/H2O2-induced carbonylation. Development of peri-implantitis resulted in a greater enhancement of free radical oxidation of blood. Isopropanol-soluble and heptane-soluble Schiff bases level was increased in blood serum as well. Similar to patients with dental implants, increased levels of carbonylated proteins was observed in blood serum at peri-implantitis. However, free radical oxidation was more intense in patients with peri-implantitis compared to patients with dental implants. Indeed, the level of heptane-soluble Schiff bases at the group with peri-implantitis was 2 times higher as compared to dental implants group, and the level of isopropanol-soluble lipid peroxidation products in inflammatory complications after implantation has reached fourfold increase compared with patients who underwent operation without complications. Conclusion. Investigated indicators of free radical oxidation may be used as markers of peri-implantitis. Among them, the most informative indicators are Schiff bases and metal-catalyzed protein oxidation.

scholarly journals Assessment of the intensity of lipid peroxidation and catalase activity in the blood-erythrocyte serum system in patients with laryngeal cancer

2019 ◽  
pp. 41-46
Author(s):  
Julia Burlaka ◽  
Sergiy Verevka ◽  
Natalia Grin
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Laryngeal Cancer ◽  
Catalase Activity ◽  
Plasma Membranes ◽  
Free Radical Oxidation ◽  
Antioxidant Defense System ◽  
Serum Samples ◽  
Diene Conjugates ◽  
Radical Oxidation

Aim: To evaluate the intensity of lipid peroxidation and the activity of catalase in patients with laryngeal cancer. Materials and Methods: Samples of blood serum and erythrocytes from 40 patients with laryngeal cancer of stages II and III and 20 healthy volunteers were investigated. Results: The level of TBA-positive products and diene conjugates in blood serum samples of stage II and III patients with laryngeal cancer was increased than that of control. In the same time catalase activity was significantly reduced. In the erythrocytes of patients in both groups the level of diene conjugates and catalase activity was higher than that of control. The level of TBA-positive products increased only in patients with III stage disease. Our results indicate the intensification of free-radical oxidation against the background of the functional stress of the antioxidant defense system in patients with laryngeal cancer, as well as the violation of the structural and functional state of erythrocyte plasma membranes, which is manifested in the activation of lipid peroxidation. This indicates about the formation of a complex of disorders, which can play a significant role in the development and deepening of the pathological process.

scholarly journals NITRIC OXIDE AND ALLYL ALCOHOL INDUCED HEPATOTOXICITY

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
M. M. Korda
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Blood Serum ◽  
Allyl Alcohol ◽  
Calcium Ionophore ◽  
Free Radical Oxidation ◽  
No Synthase ◽  
Nitrate Content ◽  
Radical Oxidation ◽  
Nos Inhibitors

<p><strong>Background.</strong> Nitric oxide (NO) is an important mediator of hepatotoxicity. NO in liver can be derived from two sources: (1) constitutive NO synthase (eNOS) in endothelial cells, and (2) inducible NO synthase (iNOS) in hepatocytes and Kupffer cells.<br /><strong>Objectives.</strong> The present study was aimed to examine the effect of nonselective NOS inhibitor (L-NAME) and selective iNOS inhibitor (1400W) on the development of allyl alcohol (AA) induced hepatitis in rats.<br /><strong>Methods.</strong> Male Wistar rats were treated with intraperitoneal injection of saline or AA and L-NAME or 1400W. NO in liver was measured by electrochemical method after eNOS stimulation by calcium ionophore. Total NOS activity and nitrite/nitrate content were measured in liver and blood serum. The activity of free radical oxidation in liver was measured by chemiluminescent method. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were assayed in blood serum<br /><strong>Results.</strong> AA increased the activity of free radical processes in liver and markers of cytolysis in serum, as well as decreased eNOS and increased iNOS activities. L-NAME considerably inhibited eNOS and augmented the necrosogenic properties of AA, whereas 1400W partially prevented liver damage.<br /><strong>Conclusion.</strong> It has been concluded that in AA intoxication NO produced from eNOS is beneficial to the liver, but NO derived from the upregulated iNOS has deleterious effect.</p><p><strong>KEY WORDS:</strong> nitric oxide, toxic hepatitis, NOS inhibitors.<br /><br /></p>

scholarly journals Reciprocity between muscarinic and nicotinic cholinoreactive structures of liver tissue in conditions, in substrates and in products of free radical oxidation of liver lipids in 5-day period of cooling in rats

2019 ◽  
Vol 17 (3) ◽  
pp. 51-58
Author(s):  
Viktor I. Tikhanov
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Membrane ◽  
Free Radical ◽  
Liver Tissue ◽  
Free Radical Oxidation ◽  
Liver Lipids ◽  
Radical Oxidation ◽  
Nicotinic Cholinoreceptors

The effects of administration of cholinotropic agents (neostigmine, hexamethonium, pilocarpine, atropine, nicotine) on liver tissue after 5-day period of cooling of rats were compared. The analysis of the conditions inducing lipid peroxidation (LPO), the analysis of the substrate components of the LPO in liver, and the evaluation of the LPO products content in 5-day period of cold loads were made. The data obtained indicate on the contradirectory effects when pilocarpine and nicotine were administered to animals, as well as after administration of atropine and hexamethonium to animals. There was the similar effects after administration of both pilocarpine and hexamethonium and both nicotine and atropine in conditions of activated LPO assessed by substrate components of LPO in the liver after 5-day cooling. On the basis of these results, a hypothesis of reciprocity between muscarinic and nicotinic cholinoreceptors located on the plasma membrane of hepatocytes in the LPO processes of the liver during the period of cold loads has been formulated.

scholarly journals Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

2019 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
Anastasiya S. Brichagina ◽  
Mariya I. Dolgikh ◽  
Larisa R. Kolesnikova ◽  
Larisa V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Biochemical Methods ◽  
Peroxidation Processes

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

Fluorescent Lipid-Peroxidation Products in Synovial Fluid

1979 ◽  
Vol 56 (1) ◽  
pp. 53-59 ◽  
Author(s):  
J. Lunec ◽  
T. L. Dormandy
Keyword(s):  
Lipid Peroxidation ◽  
Synovial Fluid ◽  
Degradation Products ◽  
Free Radical Oxidation ◽  
Aqueous Methanol ◽  
Prolonged Incubation ◽  
Radical Oxidation ◽  
Polyunsaturated Lipids ◽  
Fluorescent Compounds

1. Samples and extracts from synovial effusions were examined for the presence of fluorescent degradation products of free-radical oxidation (peroxidation). 2. Two classes of fluorescent compounds were identified and their fluorescence and physical properties are described. The fluorescence in the aqueous methanol phase of the extracts had the characteristics of conjugated Schiff bases. 3. Changes in synovial fluid and synovial fluid extracts in vitro induced by u.v. irradiation, prolonged incubation and the enzymic generation of oxidizing free radicals, with or without admixed peroxidizing arachidonic acid, and in the absence and presence of antioxidants, suggest that the fluorescent material is derived from peroxidized polyunsaturated lipids in damaged synovial tissue.

Lipid Peroxidation in Iron-Overloaded Spleens

1981 ◽  
Vol 60 (3) ◽  
pp. 295-301 ◽  
Author(s):  
A. D. Heys ◽  
T. L. Dormandy
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Iron Overload ◽  
Iron Content ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Therapeutic Implications ◽  
Dose Dependent ◽  
Increased Susceptibility

1. Iron-overloaded spleens from thalassaemic subjects showed a greatly increased susceptibility to free-radical oxidation (peroxidation) in vitro. 2. Iron content was the main but not the only variable governing susceptibility. Added ascorbate had a dose-dependent action either as an antioxidant or as a pro-oxidant, the direction of its effect depending mainly on the degree of iron overload. This has therapeutic implications.

scholarly journals Specific features of biological oxidation processes in helminthiases of horses

2020 ◽  
Vol 61 (3) ◽  
pp. 126-130
Author(s):  
Larisa V. Pilip ◽  
◽  
Olga V. Byakova ◽  
Maria E. Kazakova ◽  
◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The Body ◽  
Luminous Intensity ◽  
Radical Oxidation ◽  
Pathological Conditions ◽  
Oxidized Products

The chemical processes occurring in the animal body under pathological conditions are of interest to researchers. The objective of the research was to study the indicators of lipid peroxidation and antioxidant activity in horses infested with helminthiases and treated with anthelmintic paste with an antioxidant. The study has proved the role of parasitic infestation in accelerating the processes of free radical oxidation, which is manifested by a decrease in the light sum of radicals (36.62-39.21), the maximum luminous intensity (6.13-6.98) and antioxidant activity (5.65-6.2) at the start of the research. Chronic invasion increases the concentration of under-oxidized products up to 38.59, and the rate of initiating free radical oxidation – up to 6.95, the values of antioxidant activity being stably low (5.51-5.91). An important role in the regulation of lipid peroxidation in horses during helminthiases, as well as in the process of deworming, is played by the antioxidants contained in anthelmintic preparations. The antioxidant santohin included as a compound in the «Alezan» preparation suppresses enhanced peroxidation and activates the antioxidant protection of the body, which is reflected in a decrease in the light sum of the radicals to as low as 26.73, a maximum luminous intensity to – 3.76, and an increase in antioxidant activity – up to 7.16.

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