scholarly journals Microbial community with the ability to biodegradation perchlorate in a bio-electrochemical reactor

2019 ◽  
Vol 118 ◽  
pp. 01021
Author(s):  
Yu Zhong ◽  
Li He ◽  
Guangyi Fu ◽  
Youze Xu ◽  
Lixia Xie ◽  
...  
Keyword(s):  
Microbial Community ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Removal Rate ◽  
Electrochemical Reactor ◽  
Hunan Province ◽  
Sequencing Analysis ◽  
Initial Concentration ◽  
Perchlorate Reduction ◽  
Class Level

In this study, the sediments from the Liuyang River (Hunan Province, China) were used as an inoculum to bio-reduction perchlorate in a bio-electrochemical reactor (BER). The efficient degradation of perchlorate was found in the BER by utilizing the hydrogen as electron donor. When the current intensity was 10 mA and HRT was 72 h, the removal rate of perchlorate (initial concentration was 5 mg/L) reached 84.13% and a removal flux of 178.68 mg/m2·d was achieved. High-throughput sequencing analysis confirmed that the biofilm in the reactor had been successfully acclimated, and the system could achieve perchlorate reduction effectively. Firmicutes and Bacteroidetes were the dominant phyla during inoculation phase, and Actinobacteriria, Proteobacteria, and Tenericutes also constituted a low proportion in the biofilm. Bacilli and Clostridia were dominant at class-level both in inoculum and biofilm, with the relative abundance about 56%-72% and 17%-23%, respectively. These results confirmed that the biofilm in the BER system had been successfully formed, and the BER system could biodegradation perchlorate effectively.

scholarly journals Nitrogen Removal Efficiency and Microbial Community Analysis of a High-Efficiency Honeycomb Fixed-Bed Bioreactor

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1832
Author(s):  
Jie Xu ◽  
Chao Zhu ◽  
Yi Liu ◽  
Guanghui Lv ◽  
Changyan Tian ◽  
...  
Keyword(s):  
Microbial Community ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
High Efficiency ◽  
Removal Rate ◽  
Fixed Bed ◽  
Microbial Community Analysis ◽  
Sequencing Analysis ◽  
Fixed Bed Bioreactor

Based on the concept of microbial community multi-processing in integrated spatial bacterial succession (ISBS), this study constructs a highly efficient cellular fixed-bed bioreactor that follows the growth of biological flora in the wastewater treatment process. The reactor is organically partitioned based on synergistic laws and in accordance with environmental and microbial metabolic changes, and sewage is subjected to unitized and specialized biological treatment under direct current conditions. The results show that the ISBS reactor exhibits stable nitrogen removal performance under a low-carbon source. Compared with traditional sewage biochemical treatment technology, the microbial concentration is increased by 2–3 times and even up to 12 times, and the ammonia nitrogen removal rate is maintained at 99%. The removal rate reaches 90% (hydraulic retention time of 14 h). High-throughput sequencing analysis based on 16S rDNA reveals the microbial community structure succession at different depths of the same section of the reactor. The microbial community is rich under the influence of environmental factors and exhibits different responses. The intervals vary. An analysis of the microbial community function explains why the ISBS reactor has high nitrogen removal efficiency.

scholarly journals High nitrogen concentration alter microbial community in Allium fistulosum rhizosphere

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241371
Author(s):  
Chen Zhao ◽  
Haifeng Ni ◽  
Lin Zhao ◽  
Lin Zhou ◽  
Orlando Borrás-Hidalgo ◽  
...  
Keyword(s):  
Microbial Community ◽  
Relative Abundance ◽  
Growth And Development ◽  
High Throughput Sequencing ◽  
Nitrogen Concentration ◽  
Soil Microbiome ◽  
Allium Fistulosum ◽  
Sequencing Analysis ◽  
Nitrogen Application ◽  
Welsh Onion

Welsh onion (Allium fistulosum L.) constitutes an important plant species cultivated in China due the benefits and applications in different areas. Moreover, nitrogen is an essential nutrient during the growth and development of plant. Here, we present the effects of nitrogen on soil microbiome in welsh onion plants. We used High-throughput sequencing analysis to determine the diversity and abundances of microbes associated to soil rhizosphere in welsh onion under the influence of nitrogen application. Nitrogen application significantly influenced in the diversity of fungal community. The relative abundance of Orbiliomycetes increased with the nitrogen concentration. Nitrogen application did not affect the diversity of bacterial community, whereas the relative abundance of Acidobacteria_Gp2, Verrucomicrobiae and Sphingobacteriia decreased with the nitrogen condition. In this work, we introduced evidences of the effect of nitrogen fertilization on microbial community in welsh onion rhizosphere, and the change of microbial community may interfere the growth and development of welsh onion.

Characterization of microbial community in cold-chain hairtail fish by high-throughput sequencing technology

2021 ◽  
Author(s):  
Jiali Xing ◽  
Xiaorong Xu ◽  
Xiaohu Luo ◽  
Ruihang Zheng ◽  
Lingyan Mao ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Flora ◽  
Diversity Indices ◽  
Cold Chain ◽  
Class Level ◽  
The Family ◽  
Dominant Bacteria

Abstract: High-throughput sequencing was used to analyze the microbial communities in the muscle samples of hairtail fish to study their diversity and dynamic changes during cold-chain circulation. The results showed that the richness and diversity of the microbial community in hairtail fish had a transient decline in 0–24 h and decreased after the first rise during 24–216 h. The diversity and richness of bacteria in cold-chain hairtail fish reached the maximum at 168 h. The Shannon and Simpson diversity indices of the bacteria were 2.96 and 0.16, respectively, and their ACE and Chao1 richness indices were 254.84 and 155.10, respectively. In addition, the dominant bacteria were Proteobacteria in the phylum level, Gammaproteobacteria in the class level, Pseudomonadales in the order level, Pseudomonadaceae in the family level, and Pseudomonas in the genus level, and their relative abundance were 80.52%, 72.11%, 76.68%, 23.25%, and 53.50%, respectively. In this study, the structure of bacterial flora and the dominant bacteria in cold-chain hairtail fish were analyzed by high-throughput sequencing to provide a basis for exploring how to maintain the freshness of hairtail fish and for predicting the shelf-life of hairtail fish.

scholarly journals Analysis of Microbial Diversity and Variation Law During Xiaoqu Baijiu Fermentation using High-throughput Sequencing Technology

2021 ◽  
Author(s):  
Qing Wang ◽  
Xiaoqing Xiang ◽  
PengFei Wu ◽  
Guoqiang Han
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
High Throughput ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Sequencing Technology ◽  
Theoretical Support ◽  
Brewing Process ◽  
Dominant Bacteria ◽  
Brewing Time

Abstract In this study, high-throughput sequencing (HTS) was used to compare and analyze the microbial diversity and variation law during the brewing process of xiaoqu Baijiu. The results showed that 34 phyla, 378 genera of bacteria and 4 phyla, 32 genera of fungi were detected. At the phylum level, Firmicutes, Proteobacteria, Bacteroidetes, Ascomycota and Bacteroidetes were the dominant groups. During the brewing process of xiaoqu Baijiu, the dominant bacteria were Weissella and unidentified Rickettsiales 2 days before brewing and Lactobacillus 3 days after brewing until the end of brewing. The dominant fungi were Rhizopus, Saccharomyces and Issatchenkia. The relative abundance of Rhizopus decreased with the extension of brewing time, while the relative abundance of Saccharomyces increased and became the dominant bacteria after the second day of brewing. This study revealed the diversity and variation of microbial community in the brewing process of xiaoqu Baijiu, and provide theoretical support and lay the foundation for future study on the contribution of microbial metabolism during brewing of xiaoqu Baijiu, thereby promote the development of xiaoqu baijiu industry.

Analysis of microbial changes in the rhizosphere of unplanted and planted Atractylodes lancea via high-throughput sequencing

2020 ◽  
Author(s):  
Yan Xu ◽  
Junfeng Niu ◽  
Lijun Chen ◽  
Xiaoqiang Wu ◽  
Zhongmin Dong ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Community Diversity ◽  
Continuous Cropping ◽  
Pests And Diseases ◽  
Atractylodes Lancea ◽  
Bacteria And Fungi ◽  
The Relationship

Abstract Background Atractylodes lancea is a traditional Chinese medicine, which typically requires more than 3–4 years of continuous cropping to obtain the underground medicinal components. With continuous cropping years, the quality and yields of A. lancea medicinal materials decrease, while pests and diseases increase. These aspects are intimately correlated with rhizospheric microorganisms. Methods This research paper employed high-throughput sequencing for its detection in soil that was cultivated for three years and never cultivated to clarify the relationship between the microbial diversity of the rhizosphere and continuous A. lancea cropping. Results The rhizosphere microbial community was altered following the continuous cropping of A. lancea. The bacterial diversity and richness were observed to decrease, while the fungal community diversity increased, and richness decreased. The total OUTs of the soil bacteria and fungi of unplanted and planted A. lancea were 59.58% and 37.65%, respectively. At the phylum level, the relative abundance of Proteobacteria, Gemmatimonadetes, Acidobacteria and Chloroflexi decreased, whereas the relative abundance of Mortierellomycota increased. At the genus level, Bradyrhizobium, Striaticonidium, Dactylonectria, Sphingomonas, Burkholderiaceae, Rhodanobacter, Arthrobacter, Scleroderma, Mortierella and Penicillium were significantly different between the two sample groups. Conclusions Our results revealed that following the cultivation of A. lancea, the rhizospheric microbial community was altered. This study preliminarily determined the

Microbial community in anoxic–oxic–settling–anaerobic sludge reduction process revealed by 454 pyrosequencing analysis

2014 ◽  
Vol 60 (12) ◽  
pp. 799-809 ◽  
Author(s):  
Xinqiang Ning ◽  
Wenwen Qiao ◽  
Lei Zhang ◽  
Xu Gao
Keyword(s):  
Microbial Community ◽  
Relative Abundance ◽  
454 Pyrosequencing ◽  
Reduction Rate ◽  
Reduction Process ◽  
Anaerobic Sludge ◽  
Ammonia Oxidizing Bacteria ◽  
Sludge Reduction ◽  
Class Level ◽  
Fermentative Bacteria

Modification of the anoxic–oxic (AO) process by inserting a sludge holding tank (SHT) into the sludge return line forms an anoxic–oxic–settling–anaerobic (A+OSA) process that can achieve a 48.98% sludge reduction rate. The 454 pyrosequencing method was used to obtain the microbial communities of the AO and A+OSA processes. Results showed that the microbial community structures of the 2 processes were different as a result of the SHT insertion. Bacteria assigned to the phyla Proteobacteria and Bacteroidetes commonly existed and dominated the microbial populations of the 2 processes. However, the relative abundance of these populations shifted in the presence of SHT. The relative abundance of Proteobacteria decreased during the A+OSA process. A specific comparison at the class level showed that Sphingobacteria was enriched in the A+OSA process. The result suggested that the fermentative bacteria Sphingobacteria may have key functions in reducing the sludge from the A+OSA process. Uncultured Nitrosomonadaceae gradually became the dominant ammonia-oxidizing bacteria, and the nitrite-oxidizing bacterium Nitrospira was enriched in the A+OSA process. Both occurrences were favorable for stabilized nitrogen removal. The known denitrifying species in the A+OSA process were similar to those in the AO process; however, their relative abundance also decreased.

scholarly journals High-throughput sequencing analysis of the microbial community in coastal intensive mariculture systems

2018 ◽  
Vol 83 ◽  
pp. 93-102 ◽  
Author(s):  
Jian-Hua Wang ◽  
Jian Lu ◽  
Yu-Xuan Zhang ◽  
Jun Wu ◽  
Cui Zhang ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sequencing Analysis

scholarly journals Analysis of microbial community and pollutants removal performance in the simulated reaction pool by immobilized microbe technology

2019 ◽  
Vol 79 ◽  
pp. 03018 ◽  
Author(s):  
Qing Fang ◽  
Hai-fang Tang ◽  
Ping Xian ◽  
Jun-cen Bai
Keyword(s):  
Microbial Community ◽  
High Throughput Sequencing ◽  
Water Environment ◽  
Quality Standard ◽  
Control Group ◽  
Sequencing Analysis ◽  
Class Iii ◽  
Overlying Water ◽  
Pollutants Removal ◽  
Dominant Bacteria

In this lab-scale simulation, the performance of aerated immobilized microbe (IM) in in-situ pond sedimentation remediation, and the microbial community was studied. It is known for the results that total organic matter (TOM), total nitrogen (TN) and total phosphorus (TP) in the sediment can be effectively reduced by aerated immobilized microbe technology. The polluted raw water, classified as inferior Class-Ⅴ according to the surface water environment quality standard (GB3838-2002), upgraded to Class-III in 30-days remediation with TOM, TN and TP removal rates of 33.35%, 43.35% and 23.20%, respectively. In contract, the control group without the assistant of aerated immobilized microbe still remains at Class-IV. High-throughput sequencing analysis reveals that immobilized microbe technology could improve the species diversity and abundance of the flora, and enhance the similarity between the sediment and the overlying water in the system. The dominant bacteria of the overlying water were Perlucidibaca, Limnohabitans, Bacillus, Hydrogenophaga, Mycobacterium etc. And the dominant bacteria of the sediment were Methanolinea, Methanoregula, Candidatus Competibacter, Methanosaeta etc. Total phosphorous, certified by the redundancy analysis, is the key environmental factor of microbial community structure in both overlying water and sediment.

scholarly journals Performance and Microbial Community of Different Biofilm Membrane Bioreactors Treating Antibiotic-Containing Synthetic Mariculture Wastewater

Membranes ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 282
Author(s):  
Huining Zhang ◽  
Xin Yuan ◽  
Hanqing Wang ◽  
Shuoqi Ma ◽  
Bixiao Ji
Keyword(s):  
Microbial Community ◽  
Membrane Fouling ◽  
High Throughput Sequencing ◽  
Removal Rate ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Membrane Bioreactors ◽  
Resistant Bacteria ◽  
Fouling Mitigation ◽  
Maximum Removal

The performance of pollutant removals, tetracycline (TC) and norfloxacin (NOR) removals, membrane fouling mitigation and the microbial community of three Anoxic/Oxic membrane bioreactors (AO-MBRs), including a moving bed biofilm MBR (MBRa), a fixed biofilm MBR (MBRb) and an AO-MBR (MBRc) for control, were compared in treating antibiotic-containing synthetic mariculture wastewater. The results showed that MBRb had the best effect on antibiotic removal and membrane fouling mitigation compared to the other two bioreactors. The maximum removal rate of TC reached 91.65% and the maximum removal rate of NOR reached 45.46% in MBRb. The addition of antibiotics had little effect on the removal of chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N)—both maintained more than 90% removal rate during the entire operation. High-throughput sequencing demonstrated that TC and NOR resulted in a significant decrease in the microbial diversity and the microbial richness MBRs. Flavobacteriia, Firmicutes and Azoarcus, regarded as drug-resistant bacteria, might play a crucial part in the removal of antibiotics. In addition, the dynamics of microbial community had a great change, which included the accumulation of resistant microorganisms and the gradual reduction or disappearance of other microorganisms under antibiotic pressure. The research provides an insight into the antibiotic-containing mariculture wastewater treatment and has certain reference value.

Sign in / Sign up

Export Citation Format

Share Document