scholarly journals Feasibility study of non-contact measurement in mechanical verification method for dissolution instrument

2021 ◽  
Vol 257 ◽  
pp. 02058
Author(s):  
Wei Zhou ◽  
Mi Yao
Keyword(s):  
Measurement Method ◽  
Mechanical Verification ◽  
Verification Method ◽  
Contact Measurement ◽  
Important Means ◽  
Good Measurement ◽  
Accuracy And Repeatability

Dissolution instrument is an effective alternative method to study and evaluate the bioavailability of drugs in vitro, and an important means to ensure and evaluate whether the production technology and quality of solid oral preparations are reasonable and stable. It is found that the mechanical properties of the instrument will affect the determination of the drug, which will lead to the deviation of the determination results. Some methods of mechanical verification of dissolution instrument are analyzed and the application of non-contact measurement method is explored in mechanical verification. Experimental results show that the proposed non-contact measurement method has good measurement accuracy and repeatability.

scholarly journals Evaluation of sperm quality of Erythrolamprus poecilogyrus sublineatus (Cope, 1860) (Serpentes, Dipsadidae)

2017 ◽  
Vol 77 (3) ◽  
pp. 553-557
Author(s):  
A. C. Silva ◽  
A. S. Varela Junior ◽  
T. F. Cardoso ◽  
E. F. Silva ◽  
D. Loebmann ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Coastal Region ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
Sperm Analysis ◽  
Pampa Region

Abstract Erythrolamprus poecilogyrus sublineatus (Cope, 1860), is a species widely distributed in the Pampa Domain, occurring in Rio Grande do Sul, Argentina and Uruguay, mainlyin the pampa region. In the coastal region of southern Brazil this is serpent is considered one of the most abundant. The purpose of the present study is to describe the techniques of sperm evaluation in vitro for E. poecilogyrus sublineatus in the coastal plain of Rio Grande do Sul, Brazil. After laparatomy the efferent vases were collected and the semen was diluted in 1ml Beltsville Thawing Solution. The characteristics of motility, membrane integrity, mitochondria, acrosome, DNA, cell viability and cellular functionality were evaluated. Fluorescent probes were used for the evaluation of sperm structure in epifluorescence microscope. With the techniques described, it was possible to identify intact and injured cells, enabling the determination of cell characteristics for the spring season (October and November). It was observed in the analyses that 80% of sperm cells were mobile and that 84.1 ± 8.0% of sperm membranes were intact. The standards found were of 48 ± 13.8% of intact acrosome, 73.6 ± 6.0 of perfect DNA and of 91.8 ± 4.0 of functional mitochondria. Thus, these values from the sperm analysis can be used as standards for the species Erythrolamprus poecilogyrus sublineatus.

scholarly journals METHODS FOR ASSESSMENT OF THE SPECIFIC ACTIVITY OF DRUGS FOR PREVENTION OF HEPATITIS B

2017 ◽  
Vol 62 (5) ◽  
pp. 233-240
Author(s):  
E. L. Postnova ◽  
N. V. Shalunova ◽  
K. A. Sarkisyan ◽  
A. A. Movsesyants
Keyword(s):  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Specific Activity ◽  
Elisa Method ◽  
Normative Documents ◽  
Immunologic Activity

The immunologic activity (specific activity) is one of the main indicators of quality of vaccines for prophylaxis of hepatitis B, along with their safety. Retrospective analysis of the use of laboratory methods for assessment of specific (immunogenic) activity of modern vaccines against hepatitis B using indicators was carried out: in vitro method based on evaluation of HBsAg content and in vivo method based on evaluation of immunogenic activity in mice. Both methods are standardized and described in normative documents on the vaccines against hepatitis B of domestic production registered in the Russian Federation. Indicators of specific (immunogenic) activity of vaccines against hepatitis B were used to investigate more than 170 vaccine series using the ELISA method in the period from 2013 to 2015. The obtained control results confirmed the expediency and efficiency of enzyme immunoassay for determination of HBsAg content, as well as permissibility of use of ready sets of the Murex HBsAg Version 3 test systems for testing vaccines against hepatitis B by the ELISA method. Analysis of the results of laboratory control of series of vaccines against hepatitis B using a biological method for immunogenicity evaluation based on ED50 analysis confirms persistently high immunogenic activity of the Russian commercial vaccines intended for prophylaxis of hepatitis B. The confirmed comparability of methods allows the number of in vivo tests to be further reduced in favor of the enzyme immunoassay authentically characterizing the produced drug.

scholarly journals Determination of the protein quality of almonds ( Prunus dulcis L.) as assessed by in vitro and in vivo methodologies

2019 ◽  
Vol 7 (9) ◽  
pp. 2932-2938 ◽  
Author(s):  
James D. House ◽  
Kristen Hill ◽  
Jason Neufeld ◽  
Adam Franczyk ◽  
Matthew G. Nosworthy
Keyword(s):  
Protein Quality ◽  
Prunus Dulcis

scholarly journals The Importance of Self-Determination to the Quality of Life of People with Intellectual Disability: A Perspective

2020 ◽  
Vol 17 (19) ◽  
pp. 7121
Author(s):  
Michael L. Wehmeyer
Keyword(s):  
Quality Of Life ◽  
Intellectual Disability ◽  
Developmental Disabilities ◽  
Self Determination ◽  
Intellectual And Developmental Disabilities ◽  
Theoretical Frameworks ◽  
The Core ◽  
Important Means

There is both an intuitive and theoretical link between self-determination and quality of life for people with intellectual and developmental disabilities. Theoretically, definitions of self-determination have framed the construct with regard to its contribution to a person’s overall quality of life, while theoretical frameworks of quality of life have included self-determination among the core dimensions contributing to enhanced quality of life. These theoretical linkages have been supported by research on the quality of life and self-determination of people with intellectual and developmental disabilities and the relationships between these constructs. This article provides an overview of theoretical frameworks of self-determination, their relationship with theoretical frameworks of quality of life, and research pertaining to these constructs with people with intellectual and developmental disabilities. It is concluded that self-determination and quality of life are important constructs in designing supports that enable people with intellectual and developmental disabilities and that an important means to enhance the quality of life of people with intellectual and developmental disabilities is to promote and enable people to be self-determined.

In vitro Efficacy of Deltamethrin against Hyalomma anatolicum anatolicum

2019 ◽  
Vol 15 (01) ◽  
pp. 55-56
Author(s):  
Pratibha Thakur ◽  
R K Bagherwal ◽  
A K Jayraw ◽  
A K Soni
Keyword(s):  
Tick Control ◽  
Boophilus Microplus ◽  
Acaricide Resistance ◽  
Hyalomma Anatolicum Anatolicum ◽  
Nerve Ganglion ◽  
Cattle Health ◽  
College Farm

Tick and tick-borne diseases such as theileriosis,babesiosis, and Anaplasmosis pose a constant threato cattle health. Rhipicephalus (Boophilus) microplus andHyalomma anatolicum are the most commonly foundcattle tick species and cause significant economic lossesto dairy and leather industries by adversely affecting themilk production and quality of hides. Tick control strategyinvolves mainly the use of synthetic acaricides which resultsin the development of acaricide resistance. Deltamethrinacts on the parasites by contact and has strong lipophilicaction thus penetrates the cuticle nerve ganglion and block the motor activity. The presentstudy was aimed at the determination of in vitro efficacyof Deltamethrin against H. anatolicum ticks collected fromhealthy cattle of college farm and nearby dairy farms.

Determination of 21-hydroxylase autoantibodies: inter-laboratory concordance in the Euradrenal International Serum Exchange Program

2015 ◽  
Vol 53 (11) ◽  
Author(s):  
Alberto Falorni ◽  
Vittorio Bini ◽  
Corrado Betterle ◽  
Annalisa Brozzetti ◽  
Luis Castaño ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Rater Agreement ◽  
Exchange Program ◽  
Area Under Roc Curve ◽  
Cutoff Levels ◽  
Commercial Kit ◽  
Selection Of

Abstract21-Hydroxylase autoantibodies (21OHAb) are markers of an adrenal autoimmune process that identifies individuals with autoimmune Addison’s disease (AAD). Quality and inter-laboratory agreement of various 21OHAb tests are incompletely known. The objective of the study was to determine inter-laboratory concordance for 21OHAb determinations.Sixty-nine sera from 51 patients with AAD and 51 sera from 51 healthy subjects were blindly coded by a randomization center and distributed to 14 laboratories that determined 21OHAb, either by an “in-house” assay (n=9) using in vitro-translatedIntra-assay coefficient of variation ranged from 2.6% to 5.3% for laboratories using the commercial kit and from 5.1% to 23% for laboratories using “in-house” assays. Diagnostic accuracy, expressed as area under ROC curve (AUC), varied from 0.625 to 0.947 with the commercial kit and from 0.562 to 0.978 with “in-house” methods. Cohen’s κ of inter-rater agreement was 0.603 among all 14 laboratories, 0.691 among “in-house” laboratories, and 0.502 among commercial kit users. Optimized cutoff levels, calculated on the basis of AUCs, increased the diagnostic accuracy of every laboratory (AUC >0.9 for 11/14 laboratories) and increased the Cohen’s κ of inter-rater agreement. Discrepancies in quantitation of 21OHAb levels among different laboratories increased with increasing autoantibody levels.The quality of 21OHAb analytical procedures is mainly influenced by selection of cutoff value and correct handling of assay materials. A standardization program is needed to identify common standard sera and common measuring units.

scholarly journals VALIDA project: Validation of allergy in vitro diagnostics assays (Tools and recommendations for the assessment of in vitro tests in the diagnosis of allergy)

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
María L. Casas ◽  
Ángel Esteban ◽  
Miguel González-Muñoz ◽  
Moisés Labrador-Horrillo ◽  
Mariona Pascal ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Allergic Patient ◽  
Specific Ige ◽  
In Vitro Tests ◽  
Consensus Document ◽  
Specific Immunoglobulin E ◽  
The Impact

AbstractIn vitro allergen-specific immunoglobulin E (IgE) detection and quantification tests are routinely performed in clinical laboratories to diagnose patients with a suspected allergy. Numerous commercial assays are available to test for allergies, but the results can vary widely, thereby influencing both diagnosis and treatment. Given the challenges posed by differences in the various assays for in vitro determination of specific IgE, a group of experts has compiled in a document a series of recommendations on the implications that the use of a certain in vitro technique may have and the impact on the management of the allergic patient that the differences between the various techniques represent. The reading and analysis of this consensus document will help to understand the implications of the change of in vitro diagnostic method in the management of the patient with allergy, in the quality of life and in the socioeconomic costs associated with the disease.

scholarly journals In vitro degradation dynamics of neutral detergent fiber and silage quality of waste from production of heart of palm of peach palm

2021 ◽  
Vol 22 ◽  
Author(s):  
Samuel Galvão de FREITAS ◽  
Geraldo Fábio Viana BAYÃO ◽  
Augusto César de QUEIROZ ◽  
Robérson Machado PIMENTEL ◽  
Carlos Magno ROCHA Junior ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Leaf Sheath ◽  
Neutral Detergent Fiber ◽  
In Vitro Degradation ◽  
Fermentation Profile ◽  
Fermentation Parameters

ABSTRACT The objective of the present work was to evaluate the chemical composition, fermentation profile, and degradation parameters of the neutral detergent fiber of three silages made with the waste from production of heart of peach of palm, which consisted of leaf, leaf sheath, and their compound (55% leaf and 45% leaf sheath). The waste was packed in experimental silos and open after sixty days of fermentation; silage samples were collected for determination of chemical composition and degradation parameters of neutral detergent fiber at 0, 3, 6, 9, 12, 24, 36, 48, 72, and 96 h of incubation in vitro. A difference was detected in the dry matter, crude protein, neutral detergent fiber, acid detergent fiber, and lignin contents between the three types of silage. The leaf silage showed a higher fractional degradation rate and a higher potentially digestible fraction of neutral detergent fiber over the 96 h of incubation. As for the fermentative parameters, silage made with the leaf showed a higher pH (3,79) and lactic acid (1,18%), acetic acid (0,39%) and propionic acid (0,24%). The butyric acid was higher for compound silage (0,012%) and ammoniacal nitrogen was higher for sheath silage (0,94%). The leaf silage displayed better chemical characteristics, fermentation parameters and in vitro degradability properties, proving to be the silage with best nutritional value for feeding ruminants.

scholarly journals Physical quality and digestibility in vitro determination of green pellet concentrate based on Indigofera zollingeriana

2018 ◽  
Vol 22 (3) ◽  
pp. 114 ◽  
Author(s):  
Andi Tarigan ◽  
Simon P. Ginting ◽  
I. I. Arief ◽  
D. A. Astuti ◽  
L. Abdullah
Keyword(s):  
Block Design ◽  
Physical Quality ◽  
Green Pellet ◽  
Randomized Block Design ◽  
Randomized Design ◽  
Indigofera Zollingeriana ◽  
Total Bacteria

<p>This study aims to determine the physical quality and digestibility of green pellet concentrate based on <em>I.</em><em> zollingeriana</em>. The composition of green pellet concentrate feed contains <em>I. zollingeriana</em> and <em>C.</em><em> calothyrsus</em> in the following combinations: 1) <em>I.</em><em> zollingeriana</em> /<em>C.</em><em> calothyrsus</em> (90/0; R0), 2) <em>I.</em><em> zollingeriana</em>/<em>C. calothyrsus</em> (75/15; R1), 3) <em>I.</em><em> zollingeriana</em>/<em>C. calothyrsus</em> (60/30; R2) and 4<em>) I.</em><em> zollingeriana</em> / <em>C. calothyrsus</em> (45/45; R3). The physical quality study of green pellet concentrate was performed using a completely randomized design with four types of green concentrate as treatments of three replications. Green pellet concentrate studies conducted in vitro rumen fermentation were performed using in a  randomized block design with four treatments and three sources of liquid rumen as a block. The results of the experiments conducted with pellet criteria containing <em>I.</em><em> zollingeriana/C. calothyrsus </em>composition (60/30; R2) have the best physical character (water activity = 0.58; particles size = 12.69 mm; degree value angles = 21.01<sup>o</sup>; collision endurance = 99.78%; endurance friction = 90.42 %; bulk density = 590 kg/m3) compared to the other treatments. The quality of diet based on digestibility in vitro, dry matter and organic material having a pattern equal to R0 is higher (P&lt;0.05) compared with other treatments, while the rough digestibility of In Vitro protein is seen R0, R1, and R2 (P&gt;0.05), but (P&lt;0.05) was higher than the treatment of R3. There was no difference of pH and total bacteria on green pellet concentrate type. The treatment of R3 has the lowest NH3, VFA and total gas concentration (P&lt;0.5) compared with R2, R1 and R0. It was concluded that concentrate green pellet of <em>I.</em><em> zollingeriana</em> composition (60%) and <em>C.calothyrsus</em> (30%) had better physical and nutritional quality.</p>

Sperm production of a pony stallion and the treatment of spermatozoa in vitro with special reference to artificial insemination of mares

1943 ◽  
Vol 33 (2) ◽  
pp. 67-73 ◽  
Author(s):  
Min Chueh Chang
Keyword(s):  
Adverse Effect ◽  
Low Temperature ◽  
Artificial Insemination ◽  
Sperm Production ◽  
New Techniques ◽  
Different Temperatures ◽  
High Level

1. The sperm production of a pony stallion kept a constant high level when the collection of sperms was performed regularly three times a week. There was no adverse effect on the stallion or on the quantity and quality of the sperms. There is a negative correlation between the volume of semen and the concentration. The total number ejaculated remains relatively constant; the volume of accessory fluids is more variable.2. The motility of horse spermatozoa after dilution with eight different kinds of chemical media and stored at different temperatures was studied: Glucose-yolk-phosphate dilutor devised by Lamhert & McKenzie and glucose-yolk-tartrate dilutor devised by the author were found to be the best for the preservation of horse sperms at low temperature. There was not much difference between those diluted samples stored for 24 hr. at 10° C. and those slowly cooled to 1° C. Concentration of the semen by centrifuge is definitely beneficial for the preservation of horse sperms.3. Pregnancies were obtained by the insemination of sperms centrifuged and kept at 1° C. for 24 hr. The sperms of one stallion can be used for a great number of mares if artificial insemination is practised. The adoption of new techniques for the determination of the time of ovulation and for the induction of ovulation is suggested for successful artificial insemination of mares.

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