Gas chromatography-mass spectrometry analysis and in vitro biological studies on fixed oil isolated from the waste pits of two varieties of Olea europaea L.

Olive oil isolated from the fruits of the Olea europaea L. is an important part of Mediterranean diet. It is known for its diverse biological actions. Furthermore, a little amount of fixed oil and other bioactive components can also be extracted from the olive seeds which are considered as byproduct of olive oil extraction. Therefore, this study was designed to analyze the fatty acid composition and to perform in vitro biological studies on fixed oil isolated from olive seeds. The fixed oil was isolated from the olive seeds of Syrian and Greek black olive fruits by using Soxhlet apparatus. The purity was checked by measuring its refractive index. Composition of two isolated oils and a commercially available virgin olive oil was determined by preparing their Methyl esters (FAME) followed by GC-MS analysis. Various in vitro assay methods were used to investigate activities such as antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), cytotoxic activity by using Brine shrimps lethality bioassay and antimicrobial activity against two bacterial strains; Staphylococcus aureus and Escherichia coli by agar well diffusion method. GC-MS analysis revealed that the two isolated oils differ quantitatively in chemical composition with oleic acid identified as the major chemical constituent (62.6% and 73.56%). Both the fixed seed oils showed a concentration dependent DPPH radical scavenging activity ranging from 8 to 76% inhibition. The oils also exhibited excellent cytotoxic activity but no antimicrobial activity was observed. The chemical composition of the isolated fixed olive seed oil is found to be almost similar to the commercially available fruit olive oil. The fixed oil from the seeds of olive fruits possesses useful biological actions. Further studies are needed to isolate and quantify their bioactive constituents.

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Chemical Composition And In-Vitro Antimicrobial Activity Of The Essential Oils Of Three Greek Achillea Species

Zeitschrift für Naturforschung C ◽

10.1515/znc-2002-3-415 ◽

2002 ◽

Vol 57 (3-4) ◽

pp. 287-290 ◽

Cited By ~ 72

Author(s):

Prokopios Magiatis ◽

Alexios-Leandros Skaltsounis ◽

Ioanna Chinou ◽

Serkos A. Haroutounian

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Pathogenic Fungi ◽

The Other ◽

Gram Negative ◽

In Vitro Antimicrobial Activity ◽

Ms Analysis

The chemical composition of the essential oils of Achillea holosericea, Achillea taygetea, Achillea fraasii was determined by GC/MS analysis. Among the ninety-five assayed constituents, camphor, borneol and 1,8-cineol were found to be the major components. The in-vitro antimicrobial activity of these essential oils was evaluated against six bacteria indicating that the first is totally inactive, while the other two possess moderate to strong activities mainly against the Gram negative strains. The essential oil of A. fraasii was also active against the tested pathogenic fungi

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Chemical Composition of Nardostachys grandiflora Rhizome Oil from Nepal – A Contribution to the Chemotaxonomy and Bioactivity of Nardostachys

Natural Product Communications ◽

10.1177/1934578x1501000668 ◽

2015 ◽

Vol 10 (6) ◽

pp. 1934578X1501000 ◽

Cited By ~ 3

Author(s):

Prabodh Satyal ◽

Bhuwan K. Chhetri ◽

Noura S. Dosoky ◽

Ambika Poudel ◽

William N. Setzer

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Cytotoxic Activity ◽

Biological Activities ◽

Mcf 7

The essential oil from the dried rhizome of Nardostachys grandiflora, collected from Jaljale, Nepal, was obtained in 1.4% yield, and a total of 72 compounds were identified constituting 93.8% of the essential oil. The rhizome essential oil of N. grandiflora was mostly composed of calarene (9.4%), valerena-4,7(11)-diene (7.1%), nardol A (6.0%), 1(10)-aristolen-9-ol (11.6%), jatamansone (7.9%), valeranal (5.6%), and cis-valerinic acid (5.7%). The chemical composition of N. grandiflora rhizome oil from Nepal is qualitatively very different than those from Indian, Chinese, and Pakistani Nardostachys essential oils. In this study we have evaluated the chemical composition and biological activities of N. grandiflora from Nepal. Additionally, 1(10)-aristolen-9-ol was isolated and the structure determined by NMR, and represents the first report of this compound from N. grandiflora. N. grandiflora rhizome oil showed in-vitro antimicrobial activity against Bacillus cereus, Escherichia coli, and Candida albicans (MIC = 156 μg/mL), as well as in-vitro cytotoxic activity on MCF-7 cells.

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Composition and in-vitro CytotoxicActivities of the Leaf Essential Oil of Beilschmiedia erythrophloia from Taiwan

Natural Product Communications ◽

10.1177/1934578x1300800135 ◽

2013 ◽

Vol 8 (1) ◽

pp. 1934578X1300800

Author(s):

Yu-Chang Su ◽

Chen-Lung Ho

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Cytotoxic Activities ◽

Anticancer Activities ◽

Leaf Essential Oil ◽

Main Components ◽

Type Apparatus

This study investigated the chemical composition and in-vitro cytotoxic activities of the essential oil isolated from the leaf of Beilschmiedia erythrophloia. The essential oil was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Fifty-five compounds were identified, representing 100% of the oil. The main components identified were β-caryophyllene (22.6%), α-humulene (21.9%), terpinen-4-ol (5.3%), cis-β-ocimene (5.1%), sabinene (5.0%) and limonene (4.5%). The anticancer activities of oil were evaluated. The results showed that the oil exhibited cytotoxic activity against human oral, liver, lung, colon, melanoma, and leukemic cancer cells.

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In Vitro Antimicrobial Activity of Essential Oil Extracted from Leaves of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le in Vietnam

Plants ◽

10.3390/plants9040453 ◽

2020 ◽

Vol 9 (4) ◽

pp. 453 ◽

Cited By ~ 8

Author(s):

Nhan Trong Le ◽

Duc Viet Ho ◽

Tuan Quoc Doan ◽

Anh Tuan Le ◽

Ain Raal ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Inhibitory Concentration ◽

Antimicrobial Activities ◽

Antiviral Effects ◽

Scientific Report ◽

Main Components ◽

Hiv 1

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.

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In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

Natural Product Communications ◽

10.1177/1934578x1200700735 ◽

2012 ◽

Vol 7 (7) ◽

pp. 1934578X1200700 ◽

Cited By ~ 2

Author(s):

Samah Djeddi ◽

Khadidja Djebile ◽

Ghania Hadjbourega ◽

Zoubida Achour ◽

Catherine Argyropoulou ◽

...

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Properties ◽

Klebsiella Pneumonia ◽

Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

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Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

Czech Journal of Food Sciences ◽

10.17221/753-cjfs ◽

2008 ◽

Vol 25 (No. 2) ◽

pp. 81-89 ◽

Cited By ~ 43

Author(s):

A. Adiguzel ◽

H. Ozer ◽

H. Kilic ◽

B. Cetin

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Inhibitory Concentration ◽

Methanol Extract ◽

Antimicrobial Activities ◽

Satureja Hortensis ◽

Bacteria And Fungi

The present work reports the in vitro antimicrobial activities of the essential oil and methanol extract from Satureja hortensis as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), γ-terpinene (18.56%), carvacrol (13.98%), and p-cymene (8.97). The essential oil of Satureja hortensis exhibited the activity against 25 bacteria, 8 fungi, and a yeast, C. albicans; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 µl/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

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GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS AND IN VITRO ANTIMICROBIAL SCREENING OF WEDELIA GLAUCA (ORTEGA) O. HOFFM. EX HICKEN

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i11.20557 ◽

2017 ◽

Vol 10 (11) ◽

pp. 109

Author(s):

Krishnavignesh L Krishnavignesh ◽

Mahalakshmipriya A ◽

Ramesh M

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Antimicrobial Activity ◽

Mass Spectrometry Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Spectrometry Analysis ◽

Chromatography Mass Spectrometry ◽

Ms Analysis

Objective: Continued resistance toward the antibiotics urges us to explore newer antibiotics. Plants are being the safer source of antibiotics with lesser or no side effects. This study was designed to study the presence of phytochemical constituents and antibacterial activity of leaf and flower extracts of Wedelia glauca against urinary tract infection causing pathogens.Methods: The plant leaves were extracted with five different solvents based on the polarity. The extraction was done using soxhalation. Antimicrobial activity was determined by agar well diffusion method for both the sample and standard. The acetone plant extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis for screening phytoconstituents.Results: Preliminary phytochemical screening revealed the presence of diverse phytoconstituents in the plant. The different extracts exhibited a considerable antimicrobial potential. Among the solvents used acetone extract showed comparably better antimicrobial activity with 100% of inhibition rate with the maximum zone of inhibition of 1.6±0.77 mm against Staphylococcus sp. and Aspergillus sp. at the concentration of 5 mg. GC-MS analysis provided 8 major peaks which revealed the existence of a variety of bioactive compounds which may attribute to the efficacy of the plant.Conclusion: W. glauca leaf and flower extracts displayed a broad spectrum of antibacterial and antifungal activity and can be considered as a potential source of newer antibiotic compounds.

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Chemical Composition and in Vitro Antibacterial Activity of the Essential Oil of Calycolpus Moritzianus (O. Berg) Burret from Mérida, Venezuela

Natural Product Communications ◽

10.1177/1934578x0800300623 ◽

2008 ◽

Vol 3 (6) ◽

pp. 1934578X0800300 ◽

Cited By ~ 2

Author(s):

Teresa Díaz ◽

Flor D. Mora ◽

Judith Velasco ◽

Tulia Díaz ◽

Luis B. Rojas ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Enterococcus Faecalis ◽

Chemical Constituents ◽

Ms Analysis ◽

In Vitro Antibacterial Activity

The chemical constituents of the essential oil obtained by hydrodistillation from the leaves of Calycolpus moritzianus (O. Berg) Burret, syn Psidium caudatum Mc Vaught, collected in November 2006 in Mérida State, Venezuela, were identified by GC-MS analysis. Thirty components (91.1% of the sample) were identified, of which the seven major ones were β-caryophyllene (21.9%), α-pinene (10.9%), viridiflorol (9.7%), β-selinene (6.1%), α-copaene (6.3%), α-selinene (5.3%) and γ-eudesmol (5.1%). The oil was found to have antibacterial activity against Staphylococcus aureus ATCC (6538) and Enterococcus faecalis ATCC (29212), with MIC values of 60 μg/mL and 180 μg/mL, respectively.

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Chemical Composition and in vitro Antimicrobial Activity of the Essential Oils of Two Helichrysum Species from Tanzania

Zeitschrift für Naturforschung C ◽

10.1515/znc-2004-5-614 ◽

2004 ◽

Vol 59 (5-6) ◽

pp. 368-372 ◽

Cited By ~ 52

Author(s):

Christos Bougatsos ◽

Olipa Ngassapa ◽

Deborah K. B. Runyoro ◽

Ioanna B. Chinou

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Pathogenic Fungi ◽

Caryophyllene Oxide ◽

In Vitro Antimicrobial Activity ◽

Aerial Parts ◽

Main Components

The chemical composition of the essential oils obtained from the aerial parts of Helichrysum cymosum and H. fulgidum, from Tanzania, were analyzed by GC and GC/MS. A total of sixty-five compounds, representing 92.4% and 88.2% of the two oils, respectively, were identified. trans-Caryophyllene, caryophyllene oxide, β-pinene, p-cymene, spathulenol and β- bourbonene were found to be the main components. Furthermore, the oils were tested against six gram (±) bacteria and three pathogenic fungi. It was found that the oil of H. fulgidum exhibited significant antimicrobial activity, while the oil of H. cymosum was not active at all.

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Antimicrobial Activity of Differently Concentrated Nanoparticle Dispersions

Nano Hybrids and Composites ◽

10.4028/www.scientific.net/nhc.13.239 ◽

2017 ◽

Vol 13 ◽

pp. 239-247 ◽

Cited By ~ 1

Author(s):

Georgy A. Frolov ◽

Yakov N. Karasenkov ◽

Alexander A. Gusev ◽

Olga V. Zakharova ◽

Anna Yu. Godymchuk ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Dental Plaque ◽

Dispersion Medium ◽

Antimicrobial Properties ◽

Dispersed Phase ◽

Colloidal Solutions ◽

Nanoparticle Dispersions ◽

New Antibiotics

Preparation of nontoxic antimicrobial solutions is a priority in the development of new antibiotics. The in vitro effect of the chemical composition of the dispersed phase and dissolved solids in the dispersion medium on the antimicrobial properties of aqueous colloidal solutions of nanoparticles of a number of metals and metal oxides against microorganisms of different species - bacterial dental plaque, Bacillus cereus, and spores of vaccine strain Bacillus anthracis STI - 1 were studied in this work.

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