scholarly journals Comparison of Maternal Serum Levels and Placental mRNA Levels of Dickkopf-1 in Preeclamptic and Normal Pregnant Women at Delivery

2021 ◽  
Vol 81 (11) ◽  
pp. 1247-1255
Author(s):  
Mariz Kasoha ◽  
Zoltan Takacs ◽  
Lena Fackiner ◽  
Christoph Gerlinger ◽  
Panagiotis Sklavounos ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
Maternal Serum ◽  
Mrna Levels ◽  
Mortality And Morbidity ◽  
Significant Difference ◽  
Fetal Complications ◽  
Development Processes ◽  
Dickkopf 1

Abstract Background Preeclampsia remains a major cause of perinatal and maternal mortality and morbidity worldwide. Wnt/β-catenin signaling is known to be critically involved in placenta development processes. Dickkopf-1 (DKK1) is a key regulator of this transduction pathway. The aim of this study is to compare maternal serum DKK1 levels and placental mRNA levels of DKK1 and β-catenin in preeclamptic and normal pregnant women at delivery. Methods The present study included 30 women with preeclampsia and 30 women with normal pregnancy. Maternal serum DKK1 levels were measured by ELISA. Placental mRNA levels of DKK1 and β-catenin were detected using RT-PCR. Results Decreased maternal serum DKK1 levels were associated with worse maternal and fetal complications including HELLP syndrome, determination of one or more pathological symptom and IUGR diagnosis. No significant difference in maternal serum DKK1 levels was reported between preeclamptic women and women with normal pregnancy. Placental mRNA DKK1 levels were lower in preeclamptic women compared with normal pregnant women. Placental mRNA β-catenin levels showed no significant difference between two groups. Conclusions Our findings reported the aberrant placental mRNA DKK1 levels in patients with preeclampsia. In addition, worse preeclampsia features were associated with decreased maternal serum DKK1 levels. Hence, aberrant Wnt/β-catenin signaling might present a plausible mechanism in preeclampsia pathogenicity. Dysregulated expression of DKK1 at gene level in the placenta but not at protein level in the maternal serum might confirm the notion that preeclampsia is a type of placenta-derived disease.

scholarly journals Maternal Serum Melatonin Increases During Pregnancy and Falls Immediately After Delivery Implicating the Placenta as a Major Source of Melatonin

2021 ◽  
Vol 11 ◽  
Author(s):  
Haroon Ejaz ◽  
Juliana K. Figaro ◽  
Andrea M. F. Woolner ◽  
Bensita M. V. Thottakam ◽  
Helen F. Galley
Keyword(s):  
Pregnant Women ◽  
Physiological Role ◽  
Placental Tissue ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
Maternal Serum ◽  
Major Metabolite ◽  
Elective Cesarean Section ◽  
Elective Cesarean ◽  
In Pregnancy

Melatonin is a neuroendocrine hormone which regulates circadian rhythm and is also an antioxidant. The role of melatonin in pregnancy is emerging. The enzymes needed for endogenous synthesis of melatonin have been identified in the placenta, although the contribution to circulating maternal melatonin in normal pregnancy is unclear. This work aimed to determine serum levels of melatonin and its major metabolite 6-hydroxymelatonin sulfate (6-OHMS) in normal pregnant women during each trimester of pregnancy, and immediately after delivery. Blood samples were obtained from a cohort of healthy pregnant women during each trimester of pregnancy (n = 26), from women scheduled for elective Cesarean section (CS) before and after delivery (n = 15), along with placental samples, and from healthy non-pregnant women as controls (n = 30). Melatonin and its major metabolite, 6-OHMS, were measured using enzyme immunoassay. Levels of serum melatonin were significantly higher during pregnancy than in non-pregnant women (P = 0.025) and increased throughout pregnancy (P < 0.0001). In women undergoing CS, serum melatonin decreased markedly 24 h after delivery (P = 0.0013). Similar results were seen for serum levels of 6-OHMS, and placental tissue 6-OHMS levels correlated with week of gestation at delivery (p = 0.018). In summary, maternal melatonin production is higher in pregnant than in non-pregnant women, increases significantly during pregnancy with highest levels in the third trimester, and decreases abruptly after delivery. These results suggest that the placenta is a major source of melatonin and supports a physiological role for melatonin in pregnancy.

scholarly journals Effect of esomeprazole on maternal serum soluble fms-like tyrosine kinase-1 and endoglin in patients with early-onset preeclampsia

2021 ◽  
Vol 99 (1) ◽  
Author(s):  
Ahmed M Abbas ◽  
Yousra M Othman ◽  
Mohamad M Abdallah ◽  
Noura H. Abd Ellah ◽  
Hanan G. Abdel Azim ◽  
...  
Keyword(s):  
Placebo Group ◽  
Tyrosine Kinase ◽  
Early Onset ◽  
Serum Levels ◽  
Maternal Serum ◽  
Termination Of Pregnancy ◽  
Elisa Testing ◽  
Significant Difference ◽  
Fetal Complications ◽  
Early Onset Preeclampsia

Objective: This study evaluates the effect of esomeprazole on the maternal serum levels of soluble fms-like tyrosine kinase-1 (sFlt-1) and soluble endoglin (sEng) in patients with early-onset preeclampsia.Methods: A randomized, double-blind, placebo-controlled trial was carried out in a tertiary University hospital between March 2018, and September 2019 (Clinical Trials.Gov: NCT03213639). The study included women between 28 and 31+6 weeks gestational age who had been diagnosed as preeclampsia without severe features. They were randomly assigned in a 1:1 ratio into an esomeprazole group, which received esomeprazole 40 mg orally once a day, and a placebo group, which received one placebo tablet daily. Blood samples were obtained to assess levels of serum sFlt-1and sEng using ELISA testing. The primary outcome was the difference between the mean serum level of sFlt-1 and sEng at the start of treatment and at the termination of pregnancy in both groups.Results: Eighty-eight patients were randomly assigned into both groups (44 in each). No statistically significant difference was found in the levels of sFlt-1 between both groups at admission and termination of pregnancy. The number of days of treatment for the esomeprazole group was slightly longer than the placebo group (11.4±9.4 vs. 10.3±6.3 days, P=0.515). No statistically significant difference in the rate of maternal and fetal complications occurred between the two groups. No side effects from the study medications were reported.Conclusions: Esomeprazole, at the dosage used in this study did not effectively lower the serum levels of sFlt-1 and sEng in patients with early-onset preeclampsia. Furthermore, it did not prolong the duration of pregnancy, nor did it decrease maternal or fetal complications.

scholarly journals Differences in Maternal Leptin Serum Levels between Normal Pregnancy and Preeclampsia

2015 ◽  
Vol 7 (1) ◽  
pp. 37 ◽  
Author(s):  
Yusrawati Yusrawati ◽  
Ratna Lestari Habibah ◽  
Rizanda Machmud
Keyword(s):  
Low Cost ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
Maternal Serum ◽  
Wilcoxon Test ◽  
Delivery Room ◽  
Singleton Pregnancy ◽  
Cross Sectional ◽  
Serum Leptin ◽  
Significant Difference

BACKGROUND: Maternal circulating leptin, placental leptin gene expression and protein levels are increased in preeclampsia as compared with those in normal pregnancy. Increased serum leptin levels in preeclampsia which correspond with increased gestational age can be used independently or with other parameters as a marker for the severity of preeclampsia. The advantages of this examination in identifying preeclampsia severity are its simplicity, fast result, low cost and applicability.METHODS: A cross-sectional study was conducted in delivery room, polyclinic of Dr. M. Djamil Hospital, and Biomedical Laboratories of Medical Faculty of Andalas University from December 2013 to April 2014. The samples were collected from 68 women with singleton pregnancy in their third trimester (>28 weeks) presenting to the polyclinic and the delivery room of Dr. M. Djamil hospital. Differences in maternal serum leptin between preeclampsia and normal pregnancy were determined using t-test and Wilcoxon test for data with and without normal distribution, respectively, where p<0.05 indicating a significant results.RESULTS: The mean maternal serum leptin levels in normal pregnancy and preeclampsia were 1588.7964 ± 8.01074 ng/mL and 1608.1725 ± 6.69579 ng/mL, respectively. Thus, there is a statistically significant difference in mean maternal serum leptin levels between the normal pregnancy and preeclampsia (p<0.05).CONCLUSION: There are significant differences in maternal serum leptin levels between women with normal pregnancy and preeclampsia.KEYWORDS: leptin, preeclampsia, pregnancy

scholarly journals Maternal Serum Matrix Metalloproteinase 14 (MMP14) in Early Onset Preeclampsia and Normal Pregnancy

2020 ◽  
Vol 86 ◽  
pp. 01004
Author(s):  
H Sumawan ◽  
Sutrisno
Keyword(s):  
Matrix Metalloproteinases ◽  
Pregnant Women ◽  
Early Onset ◽  
Normal Pregnancy ◽  
Diagnostic Value ◽  
Maternal Serum ◽  
Migration And Invasion ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Difference ◽  
Early Onset Preeclampsia

Matrix Metalloproteinases 14 presumably play for cytotrophoblast migration and invasion of the uterine wall and in the remodeling of the spiral arteries in pregnancy. Inadequate trophoblastic invasion leads to an inappropriate vascular remodeling, which generates conditions of hypoxia and increased oxidative stress in the placenta early onset preeclampsia. Therefore, it is particularly important to investigating whether MMP14 altered and can be used as biomarker of preeclampsia. There have been no studies done to measure MMP14 in serum maternal between early onset preeclampsia and normal pregnancy The purpose of this study was to analyse whether maternal serum matrix metalloproteinases 14 levels differ in early onset preeclampsia and uncomplicated pregnancies. This crosssectional study was carried out in 20 subjects with early onset preeclampsia and 20 subjects of normotensive pregnant women range 24 up to 34 weeks of gestation. The study was conducted in Margono Hospital Purwokerto, Indonesia. Level of MMP 14 was measured in maternal serum using an enzyme-linked immunosorbent assay (ELISA). The mean difference was statically analysed by independent samples T-test and ROC curve to determine sensitivity and specificity of MMP 14.Women age, gestational age, parity and body mass index showed a non significant difference between both groups. In this study level MMP 14 in serum was higher in pregnant women with preeclampsia compared to the normotensive ( 266.41 vs 46.80 pg/dl ; p<0.00). Moreover, the area under curve of serum MMP 14 was 0.936, standard error 0.043, p<0.00. The optimal cut-off value of serum MMP at 110.73 pg/dl showed a high diagnostic value in preeclampsia with a sensitivity of 90 % and a specificity of 90%. Maternal serum MMP 14 was higher in preeclampsia and the important finding is the MMP 14 probably become a marker to predict early onset preeclampsia.

scholarly journals Perbedaan Kadar C-Reactive Protein Serum Ibu pada Kehamilan Aterm Ketuban Pecah Dini dan Kehamilan Normal

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Debby Yolanda ◽  
Ariadi Ariadi ◽  
Nur Indrawati Lipoeto
Keyword(s):  
Pregnant Women ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
T Test ◽  
C Reactive Protein ◽  
Term Pregnancy ◽  
Reactive Protein ◽  
Consecutive Sampling ◽  
Significant Difference ◽  
Protein Serum

Abstrak Kehamilan dengan Ketuban Pecah Dini (KPD) masih merupakan masalah penting dalam bidang obstetri, karena berkaitan dengan penyulit atau komplikasi yang dapat meningkatkan morbiditas serta mortalitas maternal dan perinatal. Salah satu faktor risiko KPD yaitu infeksi. C-reactive protein (CRP) merupakan salah satu protein yang meningkat pada saat terjadi infeksi. Tujuan penelitian ini adalah  menentukan perbedaan kadar CRP serum ibu pada kehamilan aterm KPD dan normal. Penelitian ini merupakan penelitian observasional dengan rancangan crosssectional. Dilaksanakan di Ruang Kebidanan RSUD Dr. Achmad Mochtar Bukittinggi tahun 2014. Sampel penelitian ini adalah ibu hamil aterm dengan jumlah 60 orang yang diambil dengan Consecutive Sampling, sampel dibagi menjadi 2 kelompok yaitu ibu hamil ketuban pecah dini dan ibu hamil normal. Pemeriksaan kadar CRP dengan menggunakanmetode ELISA. Data dianalisa menggunakan t-test independent, dan nilai p<0.05 dianggap bermakna secara statistik. Rerata kadar CRP serum pada kelompok KPD adalah 12,40±0,70 dan pada kelompok hamil normal adalah 6,44+2,36. Hasil uji independen t-test menunjukan terdapat perbedaan bermakna rerata kadar CRP serum antara kelompok KPDdengan kelompok kehamilan normal (p<0,05). Kata Kunci: C-reactive protein, ketuban pecah dini, kehamilan normalAbstract Pregnancy with prematur rupture of membrane (PROM) still become an important matter in obstetric, as it relates to complication which can increase maternal and perinatal morbidity and mortality. Infection is one of many risk factors of PROM. C-reactive protein is a protein which elevated when there is an infection. The objective of this study was to determine the difference of maternal C - reactive protein serum levels in term pregnancy with prematur rupture of membrane and normal pregnancy. This is an observational study with cross-sectional design. This study takes place in maternity room of RSUD Dr. Achmad Mochtar Bukittinggi in 2014. Samples in this study are 60 aterm pregnant women which have been chosen by consecutive sampling, samples divided with pregnant women with PROM and normal pregnant women. CRP levels measured with ELISA method. Data were analyzed using analysis ofindependent t-test, and p<0.05 was considered to be significantly different. CRP serum levels mean in term pregnancy with PROM group is 12.40±0.70 and in normal pregnancy group is 6.44+2.36. Independent t-test analysis showed that there was significant difference of maternal C - reactive protein serum levels between in term pregnancy with PROMand normal pregnancy with p value < 0.05. Keywords: C – reactive protein, preterm rupture of membrane, normal pregnancy

scholarly journals The Difference in Maternal Serum Hypoxia-Inducible Factors-1α Levels between Early Onset and Late-Onset Preeclampsia

2019 ◽  
Vol 7 (13) ◽  
pp. 2133-2137 ◽  
Author(s):  
Roza Sriyanti ◽  
Johanes C. Mose ◽  
Masrul Masrul ◽  
Netti Suharti
Keyword(s):  
Early Onset ◽  
Late Onset ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
Maternal Serum ◽  
Hypoxia Inducible Factors ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Difference ◽  
Early Onset Preeclampsia

BACKGROUND: Preeclampsia can be divided into early (EOPE) and late (LOPE) onset preeclampsia. Preeclampsia is related to the failure of placentation. Accumulation of hypoxia-inducible factors (HIF)-1α is commonly an acute and beneficial respond to hypoxia, while chronically elevated is associated with preeclampsia. AIM: This study aims to evaluate the serum levels of HIF-1α in preeclampsia and normal pregnancy, and to compare the difference between early-onset and late-onset preeclampsia. METHODS: A cross-sectional comparative study was conducted among a total of 69 pregnant women at ≥ 20 weeks of gestation, were recruited at obstetrics and gynaecology department at Dr M. Djamil Padang Hospital, network hospitals, health centres. They were divided into three groups early-onset preeclampsia, late-onset preeclampsia, and normal pregnancy. Preeclampsia was diagnosed using International Guidelines. Data were analysed by SPSS 24 program; data are presented as median and range or as mean ± standard deviation. One-way ANOVA test was used to determine the relationship between HIF-1α levels with the onset of preeclampsia. RESULTS: The results showed that the mean maternal serum HIF-1α levels in early-onset preeclampsia (EOPE), late-onset preeclampsia (LOPE), and normal pregnancy were 1366.96 ± 733.40 pg/ml, 916.87 ± 466.06 pg/ml, and 716.77 ± 541.08 pg/ml. Serum HIF-1α levels were higher in early-onset preeclampsia (EOPE), and late-onset preeclampsia (LOPE) compared to normal pregnancy. Among preeclampsia patients, serum HIF-1α was higher in EOPE than LOPE women. Statistical analysis revealed a significant difference in mean maternal serum HIF-1α between early-onset preeclampsia, late-onset preeclampsia, and normal pregnancy (p < 0.05). CONCLUSION: This study concluded that there is a significantly different level of HIF-1α between in early-onset preeclampsia, late-onset preeclampsia and normal pregnancy. Early-onset preeclampsia is the highest levels of serum HIF-1α.

Interleukin-6 and Malondialdehyde in Women with Preterm Birth

2019 ◽  
Vol 15 (2) ◽  
pp. 207-212
Author(s):  
Vinita Verma ◽  
Hina Oza ◽  
Riddhi Thaker ◽  
Sunil Kumar
Keyword(s):  
Oxidative Stress ◽  
Preterm Birth ◽  
Interleukin 6 ◽  
Critical Role ◽  
Serum Levels ◽  
Maternal Serum ◽  
Full Term ◽  
Mortality And Morbidity ◽  
Term Birth ◽  
Weak Negative Correlation

Background: Preterm Birth (PTB) is one of the main causes of neonatal death and infant mortality and morbidity. The pro-inflammatory cytokine interleukin-6 (IL-6) is a major proinflammatory mediator of the host response to infection and malondialdehyde (MDA) is a marker of oxidative stress. Objective : To evaluate potential associations between IL-6 and MDA levels in women with preterm birth. Method: A total of 150 women (66 with full-term and 84 with PTB) were enrolled in this case-control study. Predesigned performas were filled through questionnaire interviews to collect data on personal, demographic, occupational, lifestyle and reproductive history. Blood samples were collected within 36 hours of delivery. Serum concentrations of IL-6 and MDA were determined in mothers with full-term and preterm birth. Results: The mean age was marginally higher; whereas BMI was slightly lower in cases (PTB) as compared to controls (full-term) subjects. Serum IL-6 and MDA levels were significantly higher in subjects with PTB than full-term birth. The data were further analyzed with respect to underweight, normal and overweight/obese BMI. In all the BMI categories, the levels of IL-6 and MDA were higher in PTB cases. Among the PTB categories, the levels of IL-6 and MDA were highest in moderate to late preterm birth. A significant positive correlation was found between IL-6 and MDA levels. There was a weak negative correlation between either IL-6 or MDA and the number of gestational weeks. Conclusion : Elevated maternal serum levels of Interleukin-6 and Malondialdehyde in preterm as compared to full-term birth might suggest that inflammation and oxidative stress play a critical role in PTB.

Denudations as paracellular routes for alphafetoprotein and creatinine across the human syncytiotrophoblast

2000 ◽  
Vol 278 (3) ◽  
pp. R677-R683 ◽  
Author(s):  
P. Brownbill ◽  
D. Mahendran ◽  
D. Owen ◽  
P. Swanson ◽  
K. L. Thornburg ◽  
...  
Keyword(s):  
Creatinine Clearance ◽  
Morphometric Analysis ◽  
Normal Pregnancy ◽  
Volume Density ◽  
Maternal Serum ◽  
Significant Difference ◽  
Rate Limiting ◽  
Paracellular Diffusion ◽  
Hydrophilic Solutes ◽  
Combined Data

We tested two hypotheses: 1) that fibrin-containing fibrinoid-filled denudations of the syncytiotrophoblast may provide a route for paracellular diffusion and 2) that placentas from women who had elevated maternal serum alphafetoprotein (MSAFP) in midgestation had raised permeability to AFP and greater denudation than in normal pregnancy. We measured AFP and creatinine clearance across term placental cotyledons from the above groups and used light microscope morphometric analysis to determine the volume density of fibrin-containing fibrinoid deposits. There was no significant difference between the two groups in terms of AFP and creatinine clearance or volume density of fibrin-containing fibrinoid deposits. The combined data showed a significant ( P < 0.05) positive correlation between creatinine clearance, but not AFP clearance, and volume density of fibrin-containing fibrinoid. We conclude that syncytiotrophoblast denudations, with associated fibrinoid, do provide a route for diffusion of small hydrophilic solutes, but that other anatomic features of the placenta are rate limiting for transfer of AFP and similarly sized molecules.

The Relationship between Use of Low Molecular Weight Heparin during Pregnancy and Risk of Peripartum Adverse Events: A Meta-Analysis

2021 ◽  
Vol 8 (11) ◽  
Author(s):  
Xiaorong Y ◽  
◽  
Shan L ◽  
Shengji S ◽  
Tao S ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Adverse Events ◽  
Pregnant Women ◽  
Low Molecular Weight Heparin ◽  
Meta Analysis ◽  
Cochrane Library ◽  
Premature Delivery ◽  
Low Molecular Weight ◽  
Significant Difference ◽  
Fetal Complications

Introduction: To summarize the trials investigated on relationship between low molecular weight heparin use during pregnancy and peripartum adverse events. Meta-analysis was performed to evaluate the effect of Low Molecular Weight Heparin (LMWH) on maternal and fetal complications. Methods: Electronic research was performed in Cochrane Library, MEDLINE and EMBASE through October 2020. The primary outcome was the incidence of maternal and fetal complications during peripartum period. RevMan 5.3 was used for data analysis. Results: 11 articles were finally included. Meta-analysis showed there was no significant difference in abortion, premature delivery, stillbirth, preeclampsia and postpartum hemorrhage events between pregnant women who used LMWH and those who not. Conclusion: Using LMWH in pregnant women does not increase pregnancy related maternal and fetal complications.

Association between abnormal maternal serum levels of vitamin B12 and preeclampsia: a systematic review and meta-analysis

2020 ◽  
Author(s):  
Farzaneh Mardali ◽  
Somaye Fatahi ◽  
Maryam Alinaghizadeh ◽  
Hamed Kord Varkaneh ◽  
Mohammad Hassan Sohouli ◽  
...  
Keyword(s):  
Systematic Review ◽  
Vitamin B12 ◽  
Pregnant Women ◽  
Data Extraction ◽  
Meta Analysis ◽  
Serum Vitamin ◽  
Serum Levels ◽  
Folic Acid Supplementation ◽  
Maternal Serum ◽  
Study Selection

Abstract Context Some evidence has shown an association between maternal vitamin B12 levels and the development of preeclampsia in pregnant women, but the relationship between preeclampsia and vitamin B12 is not clear. Objective The aim of this systematic review was to compare serum vitamin B12 levels in women with preeclampsia with those in normotensive pregnant women. Data Sources The PubMed/MEDLINE, Scopus, and Web of Science databases were searched up to August 2019, along with the reference lists of included articles. Study Selection The literature was searched for observational studies that investigated vitamin B12 levels in women with preeclampsia. Data Extraction Data were extracted independently by 2 authors. Data were pooled using a random-effects model. Results Vitamin B12 levels in women with preeclampsia were significantly lower than those in healthy women (mean, −15.24 pg/mL; 95%CI, −27.52 to −2.954; P &lt; 0.015), but heterogeneity between studies was high (I2 = 97.8%; P = 0.0103). Subgroup analyses based on folic acid supplementation, homocysteine concentrations, and gestational age at the time of sampling for vitamin B12 assessment did not identify the sources of heterogeneity. Conclusions Women with preeclampsia had significantly lower vitamin B12 concentrations than normotensive pregnant women.

Sign in / Sign up

Export Citation Format

Share Document