Differential Effects of Dietary Supplementation with Fish Oil or Soy Lecithin on Human Platelet Adhesion

1999 ◽  
Vol 82 (11) ◽  
pp. 1522-1527 ◽  
Author(s):  
Antonio Carletto ◽  
Patrizia Guarini ◽  
Simonetta Galvani ◽  
Domenico Biasi ◽  
Paolo Bellavite ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fish Oil ◽  
Platelet Adhesion ◽  
Human Platelet ◽  
Experimental Period ◽  
Inhibitory Effect ◽  
Control Group ◽  
Platelet Adhesiveness ◽  
Soy Lecithin ◽  
Group A

SummaryTo investigate the possible regulating role of ω-6 and of ω-3 fatty acids on platelet adhesiveness, we randomised 60 volunteers into three groups to take 20 ml (equivalent to 0.3 g ω-6, 3.6 g ω-3; ω-6/ω-3 ratio 0.1) per day of a fish oil supplement, or to take 25 g (equivalent to 1.5 g ω-6, 0.5 g ω-3; ω-6/ω-3 ratio 3) per day of a soy lecithin supplement, or to continue on their usual diet without any supplement (control group) for a period of 15 days. Platelet adhesion on fibrinogen-coated 96-well microtitre plates was evaluated in the resting condition and after stimulation with 2 ωM ADP or 0.02 U/ml thrombin. Compared to the values before the experimental period, the fish oil group showed a significant reduction in stimulated adhesion (with ADP: from 18.8% to 15.6%, p <0.01; with thrombin: from 24.4% to 20.8%, p <0.005), whereas no difference was noted in the resting condition (from 3.6% to 3.5%, NS). In the soy lecithin group, platelet adhesion was increased in all test conditions (with ADP: from 18.7% to 23.2%, p <0.001; with thrombin: from 24.0% to 29.9%, p <0.001; resting: from 3.5% to 6.6%, p <0.001). No significant changes were observed in the control group. A good correlation was found between platelet adhesion data and the changes in the platelet fatty acid ω-6/ω-3 ratio caused by the different supplementations. Our results indicate an inhibitory effect of fish oil rich in ω-3 fatty acids on stimulated human platelet adhesiveness and a stimulatory effect of soy lecithin rich in ω-6 fatty acids on resting and stimulated adhesion. They suggest moreover that the ω-6/ω-3 ratio is a determinant of platelet adhesion.

Dietary fish oil prevents reperfusion Ins(1,4,5)P3 release in rat heart: possible antiarrhythmic mechanism

1996 ◽  
Vol 271 (4) ◽  
pp. H1483-H1490 ◽  
Author(s):  
K. E. Anderson ◽  
X. J. Du ◽  
A. J. Sinclair ◽  
E. A. Woodcock ◽  
A. M. Dart
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Fish Oil ◽  
Saturated Fat ◽  
Inhibitory Effect ◽  
Control Group ◽  
Membrane Phospholipids ◽  
Postischemic Reperfusion ◽  
Dietary Fish ◽  
Fish Oil Supplementation

Dietary enrichment with fish oil-derived n-3 polyunsaturated fatty acids has been shown to suppress the arrhythmias that occur during postischemic reperfusion. We have recently implicated a rapid release of D-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] during postischemic reperfusion in the generation of these arrhythmias. The effects of dietary supplementation with fish oil on both cardiac Ins(1,4,5)P3 and arrhythmogenic responses to reperfusion were therefore investigated in perfused rat hearts. Comparisons were made with control and n-6 polyunsaturated or saturated fat-supplemented diets. In control hearts, reperfusion increased Ins(1,4,5)P3 levels [from 9 +/- 2 at 20 min ischemia to 26 +/- 3 counts per minute (cpm)/mg protein with 2 min of reperfusion] and produced a high incidence of ventricular tachycardia (92% VT) and ventricular fibrillation (85% VF). Dietary fish oil supplementation, which increased composition of n-3 fatty acids in myocardial membrane phospholipids, prevented the reperfusion-induced rise in Ins(1,4,5)P3 (11 +/- 1 at 20 min ischemia and 12 +/- 2 cpm/mg protein after 2-min reperfusion) and significantly suppressed reperfusion arrhythmias (38% VT, 13% VF; P < 0.01 vs. control group). Thus the inhibition of reperfusion-induced rises in Ins(1,4,5)P3 by n-3 polyunsaturated fatty acids after dietary fish oil supplementation provides a possible mechanism for the inhibitory effect of n-3 fatty acids on reperfusion-induced arrhythmias.

scholarly journals Fish oil: a potent inhibitor of platelet adhesiveness [see comments]

Blood ◽  
1990 ◽  
Vol 76 (5) ◽  
pp. 938-945 ◽  
Author(s):  
XL Li ◽  
M Steiner
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Platelet Aggregation ◽  
Eicosapentaenoic Acid ◽  
Fish Oil ◽  
Platelet Adhesion ◽  
Fatty Acid Distribution ◽  
Moderate Increase ◽  
Platelet Adhesiveness ◽  
Normal Individuals

Abstract The effect of fish oil administration on platelet function was studied in eight normal individuals, four men and four women, who received fish oil equivalent to 6 g eicosapentaenoic acid per day for a period of 25 days. Platelet aggregation, platelet adhesion, phospholipid and fatty acid distribution were measured at periodic intervals before, during, and after the period of fish oil administration. Platelet aggregation induced by arachidonic acid, adenosine diphosphate, and collagen showed a moderate increase in ED 50 in response to the administration of fish oil. Conversely, platelet adhesion to fibrinogen and collagen I, which was studied at low shear rates in a laminar flow chamber, showed a striking 60% to 65% decrease after fish oil supplementation of the diet. The change in adhesiveness could be correlated with the pseudopodia formed in response to agonistic stimulation. Scanning electron microscopic examination of adherent platelets showed an overall reduction of pseudopodia that appeared short and stubby on fish oil administration. The profile of the fatty acids extracted from plasma confirmed compliance of the volunteers with their dietary supplements. Analysis of phospholipids showed changes in sphingomyelin, lysophosphatidylcholine, and phosphatidylcholine between pseudopodia and platelet cell bodies. Fish oil administration did not affect their overall distribution except for a moderate decrease in phosphatidylethanolamine in platelet pseudopodia. Changes were also recognized in the total fatty acids extracted from platelets, affecting primarily arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid. There were no changes in platelet adhesiveness in a group of five normal individuals who received a vegetable oil supplement of equal dose and duration as that of the fish oil. We conclude from these studies that fish oil, at least when administered over a limited period of time, is an effective inhibitor of platelet adhesiveness.

scholarly journals Fish oil: a potent inhibitor of platelet adhesiveness [see comments]

Blood ◽  
1990 ◽  
Vol 76 (5) ◽  
pp. 938-945
Author(s):  
XL Li ◽  
M Steiner
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Platelet Aggregation ◽  
Eicosapentaenoic Acid ◽  
Fish Oil ◽  
Platelet Adhesion ◽  
Fatty Acid Distribution ◽  
Moderate Increase ◽  
Platelet Adhesiveness ◽  
Normal Individuals

The effect of fish oil administration on platelet function was studied in eight normal individuals, four men and four women, who received fish oil equivalent to 6 g eicosapentaenoic acid per day for a period of 25 days. Platelet aggregation, platelet adhesion, phospholipid and fatty acid distribution were measured at periodic intervals before, during, and after the period of fish oil administration. Platelet aggregation induced by arachidonic acid, adenosine diphosphate, and collagen showed a moderate increase in ED 50 in response to the administration of fish oil. Conversely, platelet adhesion to fibrinogen and collagen I, which was studied at low shear rates in a laminar flow chamber, showed a striking 60% to 65% decrease after fish oil supplementation of the diet. The change in adhesiveness could be correlated with the pseudopodia formed in response to agonistic stimulation. Scanning electron microscopic examination of adherent platelets showed an overall reduction of pseudopodia that appeared short and stubby on fish oil administration. The profile of the fatty acids extracted from plasma confirmed compliance of the volunteers with their dietary supplements. Analysis of phospholipids showed changes in sphingomyelin, lysophosphatidylcholine, and phosphatidylcholine between pseudopodia and platelet cell bodies. Fish oil administration did not affect their overall distribution except for a moderate decrease in phosphatidylethanolamine in platelet pseudopodia. Changes were also recognized in the total fatty acids extracted from platelets, affecting primarily arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid. There were no changes in platelet adhesiveness in a group of five normal individuals who received a vegetable oil supplement of equal dose and duration as that of the fish oil. We conclude from these studies that fish oil, at least when administered over a limited period of time, is an effective inhibitor of platelet adhesiveness.

scholarly journals Sodium fluoride as an ascaricide for swine raised on the ground

1969 ◽  
Vol 31 (2) ◽  
pp. 190-202
Author(s):  
Radamee Orlandi ◽  
Fernando E. Armstrong
Keyword(s):  
Sodium Fluoride ◽  
Ascaris Lumbricoides ◽  
Experimental Period ◽  
Growing Pigs ◽  
Control Group ◽  
Repeated Treatment ◽  
Treatment Trial ◽  
Cent Sodium ◽  
Group A ◽  
Group B

Two trials were conducted to test the value of sodium fluoride as an ascaricide for growing pigs kept on infested grounds. The ability of the drug to kep animals free from ascarides and the possible toxicity upon repeated treatment were also studied. Three groups of animals similar as to breeding, weight and age were used in Trial I. Group "A" served as control. Group "B" received 0.2 gm. of phenothiazine per pound or bodily weight and group "C" one per cent sodium fluoride mixed with ground feed. Except for slight variations in the procedure and the elimination of the phenothiazine treatment, Trial II was conducted in the same manner. The effectiveness of the different treatments given during the experimental period was measured by statistical analyses of the number of ascarid and non-ascarid eggs per gram of fresh rectal feces secured once every week, by the weight gains made by each animal, and by the number of Ascaris worms found upon visceral examination. The results obtained suggest that sodium fluoride at the rate of one per cent mixed in the feed every three weeks is a very satisfactory drug for killing Ascaris lumbricoides suis found in growing pigs raised on the ground. When fed repeatedly to growing pigs for periods of from 3 to 4 months it was effective in keeping the animals clean, with no toxic effects whatsoever. Phenothiazine at the rate of 0.2 gm. per pound of bodily weight administered every three weeks was found to be unreliable as an ascaricide. The data obtained during the two trials made suggest that sodium fluoride is specific against Ascaris lumbricoides suis.

The influence of mineral granulated complex on milk productivity and quality indicators of cows’ milk

2021 ◽  
pp. 33-42
Author(s):  
S. I. Nikolaev ◽  
D. A. Randelin ◽  
N. M. Kostomakhin ◽  
Yu. M. Batrakova ◽  
K. S. Arstanov ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Dry Matter ◽  
Experimental Period ◽  
Control Group ◽  
New Mineral ◽  
Lactating Cows ◽  
Nonfat Milk ◽  
Group A ◽  
Milk Solids

The purpose of the research was to study the effectiveness of the use of rations for freshly calved cows during the increasing the milk yield period with the use of new mineral granulated complexes produced by Uralchem LLC. The results of research on the replacement of the mineral part of the vitamin and mineral premix in the rations of lactating cows with the mineral granular complex developed according to two recipes have been presented in the article. Research has shown that cows of the 1st and 2nd experimental groups during the experiment period gave more milk than their herdmates from the control group by 70,8 (P ≥ 0,95) and 106,2 kg (P ≥ 0,95). Due to the higher milk yield of animals that consumed the granulated complex, the yield of milk fat was greater than that of the control analogues by 3,21 (P ≥ 0,95) and 5,17 kg (P ≥ 0,95), protein by 2,70 and 4,30 kg (P ≥ 0,95). The milk yield of cows of the 1st and 2nd experimental groups for 305 days of lactation was higher than in the control group, respectively, by 205,3 (P ≥ 0,95) and 296,8 kg (P ≥ 0,95). In the milk of cows that consumed the granulated complex, there was a tendency for a higher content of dry matter (0,09 and 0,16 %), lactose (0,01 and 0,06 %), nonfat milk solids (0,09 and 0,07 %), ash (0,05 and 0,05 %) in comparison with analogues from the control group. A similar trend has been established between groups of cows in terms of milk density, acidity and rennet coagulability. The revenue from the sale of milk produced from cows of the 1st and 2nd experimental groups was more than in the control group for the experimental period (120 days) by 2074,6 and 3344,0 rubles and for the 305 days of lactation by 7055,4 and 9950,6 rubles.

Abstract 19144: Fish Oil and Cyclooxygenase Inhibitors: a Novel Strategy to Manage Obesity-linked Dyslipidemia

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Viswanathan Saraswathi ◽  
Curtis Perriotte-Olson ◽  
Robert D Heineman ◽  
Cyrus V Desouza
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Fish Oil ◽  
Endothelial Activation ◽  
The Body ◽  
Cyclooxygenase Inhibitors ◽  
Pcr Analysis ◽  
Control Group ◽  
Cox Inhibitor ◽  
High Doses

Introduction: Dyslipidemia is a prevalent condition in obesity and type 2 diabetes. Although fish oil rich in omega-3 fatty acids (ω-3) is a widely used hypolipidemic agent, it is often required at high doses. At high doses, these fatty acids can induce oxidative stress or endothelial activation and therefore, strategies to improve their beneficial effects are needed. We previously reported that fish oil in combination with cyclooxygenase (COX) inhibitors exerts enhanced hypolipidemic and anti-inflammatory effects in low density lipoprotein receptor knock-out mice. Here, we sought to determine the effects of ω-3 fatty acids in combination with naproxen (NX), a COX inhibitor, on dyslipidemia and gene expression in subcutaneous adipose tissue (scAT) in humans. Methods: Obese dyslipidemic patients were randomly assigned to receive one of these interventions (n=8/group) for 12 wk: 1) Standard nutrition counseling (control), 2) ω-3 (2 g twice daily), 3) NX (220 mg twice daily), and 4) ω-3 (2 g twice daily) + NX (220 mg twice daily). Results: The body mass index, HOMA-IR, and plasma total, LDL, and HDL cholesterol levels were not altered significantly in any of the groups. The percent change in plasma triglycerides (TG) from baseline was 75% ( P <0.1) and 68% ( P <0.05) in ω-3 and ω3 + NX-treated subjects, respectively. Notably, 25% of subjects who received ω-3s alone did not show a reduction in TG whereas all the patients that received ω-3 + NX showed a reduction in TG. Realtime PCR analysis of scAT showed that the expression of glucose transporter 4 (GLUT-4), a marker of glucose uptake and a key regulator of glucose homeostasis was significantly reduced in ω-3 compared to control group ( P <0.01). However, combining NX with ω-3 abolished this effect. Moreover, the expression of MCP-1 and VCAM-1, markers of inflammatory response or endothelial activation, was significantly increased in ω-3 but not in ω-3 + NX group. The plasma levels of MCP-1 and E-selectin did not vary significantly in any of the groups. Conclusions: Our data reveal previously unrecognized effects of fish oil in scAT. Our data suggest that combining NX with ω-3 fatty acids will increase their effectiveness in reducing plasma TG and improve the benefits of ω-3 supplements by favorably altering gene expression in scAT.

Inhibition of DNA replication by fish oil-treated cytoplasm is counteracted by fish oil-treated nuclear extract

2002 ◽  
Vol 283 (5) ◽  
pp. C1365-C1375 ◽  
Author(s):  
Sybille Rex ◽  
Maria A. Kukuruzinska ◽  
Nawfal W. Istfan
Keyword(s):  
Fatty Acids ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Fish Oil ◽  
Corn Oil ◽  
Nuclear Extract ◽  
Inhibitory Effect ◽  
Free System ◽  
Nuclear Factors ◽  
Cytosolic Extract

We have recently noted that cells treated with fish oil and n–3-fatty acids show slower DNA replication rates than cells treated with a control emulsion or corn oil only. However, it is not clearly understood how such an effect is induced. Fish oil and its metabolites are known to have several modulating effects on signal transduction pathways. Alternatively, they may influence DNA replication by interacting directly with nuclear components. To investigate this problem in greater detail, we have studied the kinetics of DNA synthesis in a cell-free system derived from HeLa cells. Nuclei and cytosolic extract were isolated from cells synchronized in early S phase after treatment with control emulsion, corn oil, or fish oil, respectively. The nuclei were reconstituted with cytosolic extract and a reaction mixture containing bromodeoxyuridine (BrdU) triphosphate to label newly synthesized DNA. The rate of DNA synthesis was measured by bivariate DNA/BrdU analysis and flow cytometry. We show that fish oil-treated cytosol inhibits the elongation of newly synthesized DNA by ∼80% in control nuclei. However, nuclei treated with fish oil escape this inhibitory effect. We also show that addition of nuclear extract from fish oil-treated cells reverses the inhibitory effect seen in the reconstitution system of control nuclei and fish oil-treated cytosol. These results indicate that polyunsaturated fatty acids can modulate DNA synthesis through cytosolic as well as soluble nuclear factors.

The Effect of Sho-saiko-to on Concentration of Vitamin E in Serum and on Granuloma Formation in Carrageenin Cotton Pellet-Induced Granuloma Rats

1994 ◽  
Vol 22 (02) ◽  
pp. 183-189 ◽  
Author(s):  
Kazuo Yoshida ◽  
Hiromi Mizukawa ◽  
Akie Honmura ◽  
Yasuhiko Uchiyama ◽  
Shigekatsu Nakajima ◽  
...  
Keyword(s):  
Vitamin E ◽  
Lipid Peroxide ◽  
Significant Negative Correlation ◽  
Inhibitory Effect ◽  
Granuloma Formation ◽  
Control Group ◽  
Serum Lipid Peroxide ◽  
Cotton Pellet ◽  
Group A ◽  
Inflammatory Effect

The effect of Sho-saiko-to on the concentration of vitamin E in serum and on the granuloma formation in Carrageenin cotton pellet-induced rats was investigated. As a result, in the granuloma rats of Sho-saiko-to group, a significantly improved inhibitory effect on granuloma formation and a higher concentration of vitamin E in serum, cholesterol and phospholipid were observed compared to the control group. Despite this lipid-increasing action by Sho-saiko-to, the concentration of serum lipid peroxide was significantly lower than in the control group. Furthermore, a significant negative correlation between the concentration of vitamin E and granuloma weight was observed. These results suggest that vitamin E plays an important role in promoting the anti-inflammatory effect of Sho-saiko-to.

scholarly journals Effect of dietary phyto-humic preparations on lipids indices of commercial laying hen eggs

2019 ◽  
Vol 75 (03) ◽  
pp. 6197-2019 ◽  
Author(s):  
FABIOLA BUBEL ◽  
ZBIGNIEW DOBRZAŃSKI ◽  
EUGENIUSZ R. GRELA ◽  
ŁUKASZ BOBAK ◽  
MACIEJ OZIEMBŁOWSKI
Keyword(s):  
Fatty Acids ◽  
Total Cholesterol ◽  
Cholesterol Content ◽  
Atherogenic Index ◽  
Control Group ◽  
Fatty Acids Profile ◽  
Group A ◽  
Dietary Value ◽  
Thrombogenic Index ◽  
Group B

The aim of the study was to determine the effect of three different phyto-humic feed preparations on the basic qualitative characteristics of eggs, i.e. total cholesterol content and fatty acid profile of the yolk. The study was carried out on 80 Lohmann Brown (LB) laying hens in an experimental facility with a floor maintenance system on a straw bedding (5 layers/m2). A control group (K) and three experimental ones (A, B, C), with 20 birds in each (in separate pens), were created. The birds were placed in the 16th week of life, while the experimental preparations were introduced in the 22nd week of life and were administered for 43 weeks (constant 3% addition to the feed mixture).The following preparations were applied: humic-peat (H-t) in group A, humic-herbal (H-z) in group B, and humic-lucerne (H-1) in group C. The eggs for the study were collected three times, 30 eggs from each group in the following periods: 1st series – 31st week of life (peak of laying period); 2nd series – 54th week of life (later phase of laying period) and 3rd series – 65th week of life (final phase of laying period). Six samples (mean of 5 well-mixed yolks) from each group in three laying periods (360 eggs were used in total) were prepared for laboratory analyzes (lipids examination). Laboratory analyzes were performed using a chromatograph – Finnigan Focus PolarisQ manufactured by Thermo Electron with capillary columns: HP-88 by Agilent J&W (fatty acids) and Rtx-1MS by Restek (cholesterol). The applied phytohumic preparations – but only those with a share of dried lucerne or herbs – significantly (p ≤ 0.05) reduced total cholesterol content, especially in group C (10.88 mg/g yolk) compared to group K (11.94 mg/g yolk). All preparations positively influenced the fatty acids profile of yolk; there was a significant (p ≤ 0.05) increase in the concentration of n-3 PUFAs (max 2.84% in the group B), the n-6/n-3 ratio decreased significantly (most beneficial 8.33 in group B), and lipids quality improved: atherogenic index (AI) decreased significantly (p ≤ 0.05) in group A (0.32) and thrombogenic index (TI) was a significantly (p ≤ 0.05) lower in all experimental groups (0.83 – 0.84) compared to group K (0.36 and 0.87 respectively). Dietary phyto-humic preparations increased the nutritional and dietary value of hen eggs.

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