Establishing a Lymphatic Venous Anastomotic Training Model in Pig Trotters

2021 ◽  
Author(s):  
Motoi Kato ◽  
Masakazu Kurita ◽  
Daisuke Mito ◽  
Runa Nakamura ◽  
Kexin Li ◽  
...  
Keyword(s):  
Room Temperature ◽  
Lymphatic Vessels ◽  
Structural Similarity ◽  
Training Model ◽  
Practical Training ◽  
Venous Anastomosis ◽  
Surgical Model ◽  
Relevant Point ◽  
Green Fluorescent ◽  
Feasible Points

Abstract Background Lymphatic venous anastomosis (LVA) is a widely accepted surgical procedure for lymphedema. To obtain the best outcomes, surgeons should be well trained. A recent study introduced an LVA training model using pig trotters for their utility and structural similarity to human tissues. However, details regarding the utilization of anastomosis models, such as feasible points for training based on vessel anatomy, have not been clarified. Therefore, we assessed the anatomical details of lymphatic vessels and veins of trotters to establish a practical training model of LVA. Methods Ten frozen trotters were used. After thawing at room temperature, indocyanine green fluorescent lymphography was used to visualize the lymphatic course. To dissect the lymphatic vessels and veins from the distal to the proximal end, whole skins were detached thoroughly from the plantar side. Data from the lymphatic vessels and veins were collected based on their courses, diameters, and layouts to clarify adjacent points feasible for LVA training. Results Both lymphatic vessels and veins were classified into four major courses: dorsal, medial, lateral, and plantar. The majority were dorsal vessels, both lymphatic vessels and veins. The adjacent points were always found in the distal dorsum center and were especially concentrated between the metacarpophalangeal (MP) joint and central interphalangeal crease, followed by the medial and lateral sides. Conclusion The most relevant point for LVA surgical training in the trotter was the dorsal center distal to the MP joint, where parallel vessels of similar sizes were found in all cases. This practical LVA surgical model would improve surgeon skills in not only anastomosis but also preoperative fluorescent lymphography.

The construction of innovative computer practical training education model in the environment of multiple intelligences Internet of Things

2021 ◽  
pp. 1-11
Author(s):  
Yanli Bao
Keyword(s):  
Computer Science ◽  
Science Teachers ◽  
Curriculum Design ◽  
Engineering Students ◽  
Theoretical Perspective ◽  
Training Model ◽  
Practical Training ◽  
Operational Strategies ◽  
Workflow Technology ◽  
Computer Knowledge

This paper takes the application of workflow technology in a multi-smart IOT message-driven practical training execution system as the research background, analyzes the current situation and problems of the practical training execution system, and reconstructs and optimizes the original on-site practical training model and business methods according to the future development needs of the practical training execution system. This study draws on the theoretical perspective of the computer knowledge body to deeply recognize and understand the connotation of computing ability from the levels of knowledge, skills, and attitude, forms a basic understanding of computing ability through literature combing, and refines the core elements of computing ability through a combination of enterprise case study, content analysis, and questionnaire survey, strives to form a more systematic and in-depth understanding of the connotation and elements of computing ability. It also provides a reference for the clarification of computing ability training objectives of computer science teachers under the trend of intelligence. Facing the enhancement of computing ability of engineering students majoring in computer science at the undergraduate level, the objectives of computing ability cultivation are clarified, the key points of computing ability cultivation mode are refined from three levels: curriculum design, teaching operation, management, and control, and three typical computing ability cultivation modes embedded in undergraduate computer education are constructed: knowledge module combination mode, computing context experience mode, and intelligent industry-leading mode. The operational strategies and implementation paths of the models are discussed in depth. This study emphasizes that, based on the analysis of the characteristics of the trend of intelligence, the computing ability cultivation model is not static.

scholarly journals The Mycobacteriophage Ms6 LysB N-Terminus Displays Peptidoglycan Binding Affinity

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1377
Author(s):  
Adriano M. Gigante ◽  
Francisco Olivença ◽  
Maria João Catalão ◽  
Paula Leandro ◽  
José Moniz-Pereira ◽  
...  
Keyword(s):  
Mycobacterium Smegmatis ◽  
Fluorescent Protein ◽  
Cell Envelope ◽  
Structural Similarity ◽  
Lytic Cycle ◽  
Specific Lysis ◽  
Double Stranded Dna ◽  
The Family ◽  
N Terminus ◽  
Green Fluorescent

Double-stranded DNA bacteriophages end their lytic cycle by disrupting the host cell envelope, which allows the release of the virion progeny. Each phage must synthesize lysis proteins that target each cell barrier to phage release. In addition to holins, which permeabilize the cytoplasmic membrane, and endolysins, which disrupt the peptidoglycan (PG), mycobacteriophages synthesize a specific lysis protein, LysB, capable of detaching the outer membrane from the complex cell wall of mycobacteria. The family of LysB proteins is highly diverse, with many members presenting an extended N-terminus. The N-terminal region of mycobacteriophage Ms6 LysB shows structural similarity to the PG-binding domain (PGBD) of the φKZ endolysin. A fusion of this region with enhanced green fluorescent protein (Ms6LysBPGBD-EGFP) was shown to bind to Mycobacterium smegmatis, Mycobacterium vaccae, Mycobacterium bovis BGC and Mycobacterium tuberculosis H37Ra cells pretreated with SDS or Ms6 LysB. In pulldown assays, we demonstrate that Ms6 LysB and Ms6LysBPGBD-EGFP bind to purified peptidoglycan of M. smegmatis, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis, demonstrating affinity to PG of the A1γ chemotype. An infection assay with an Ms6 mutant producing a truncated version of LysB lacking the first 90 amino acids resulted in an abrupt lysis. These results clearly demonstrate that the N-terminus of Ms6 LysB binds to the PG.

scholarly journals Analysis on the Effect of Protective Equipment Training on Hospital Infection Managers

CONVERTER ◽  
2021 ◽  
pp. 211-219
Author(s):  
Yongli Zou Et al.
Keyword(s):  
Personal Protective Equipment ◽  
Protective Clothing ◽  
Training Model ◽  
Hospital Infection ◽  
Protective Equipment ◽  
Practical Training ◽  
Training Methods ◽  
Before And After ◽  
Improper Use ◽  
Expert Theory

Objectives: To analyze the effect of personal protective equipment training on new hospital infection managers. Methods: Personnel are divided into two batches by region. Adopt a diversified training model to train all personnel, finally conduct practical assessments and issue certificates. Collect information through information technology, analyze questionnaires, and understand trainees’ circumstances before and after the training. Each training batch has uniform teachers and the same training methods. Results: After the training, the trainees' proficiency in putting on and taking off protective equipment increased by 22.85%, and ability to choose protective equipment according to different working environments increased by 22.04%; 78.23% trainees believed that practical exercises should be emphasized. Taking off protective clothing was considered as the most difficult link in practical training (91.13%), followed by putting on protective clothing (70.43%). 96.24% trainees believed that this training is helpful for future work. Conclusions: It is quite necessary to implement personal protective equipment training among new hospital infection managers; where, practical training, assessment, information-based questionnaire survey, expert theory teaching have achieved good results; the training helps reduce occupational exposure-induced hospital infection, and at the same time, avoids improper use of protective materials and waste.

Presence of bone marrow–derived circulating progenitor endothelial cells in the newly formed lymphatic vessels

Blood ◽  
2005 ◽  
Vol 106 (13) ◽  
pp. 4184-4190 ◽  
Author(s):  
Piotr Religa ◽  
Renhai Cao ◽  
Meit Bjorndahl ◽  
Zhongjun Zhou ◽  
Zhenping Zhu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Fluorescent Protein ◽  
Bone Marrow Cells ◽  
Lymphatic Vessels ◽  
Growth Factor Receptor ◽  
Donor Bone Marrow ◽  
Green Fluorescent ◽  
Corneal Lymphangiogenesis ◽  
Marrow Cells

Bone marrow (BM)-derived circulating endothelial precursor cells (CEPCs) have been reported to incorporate into newly formed blood vessels under physiologic and pathologic conditions. However, it is unknown if CEPCs contribute to lymphangiogenesis. Here we show that in a corneal lymphangiogenesis model of irradiated mice reconstituted with enhanced green fluorescent protein (EGFP)-positive donor bone marrow cells, CEPCs are present in the newly formed lymphatic vessels. Depletion of bone marrow cells by irradiation remarkably suppressed lymphangiogenesis in corneas implanted with fibroblast growth factor-2 (FGF-2). Further, transplantation of isolated EGFP-positive/vascular endothelial growth factor receptor-3-positive (EGFP+/VEGFR-3+) or EGFP+/VEGFR-2+ cell populations resulted in incorporation of EGFP+ cells into the newly formed lymphatic vessels. EGFP+/CEPCs were also present in peritumoral lymphatic vessels of a fibrosarcoma. These data suggest that BM-derived CEPCs may play a role in “lymphvasculogenesis.”

scholarly journals High-temperature ferromagnetic semiconductor with a field-tunable green fluorescent effect

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Bowen Zhou ◽  
Qing Zhao ◽  
Zhehong Liu ◽  
Xudong Shen ◽  
Xubin Ye ◽  
...  
Keyword(s):  
Room Temperature ◽  
Energy Gap ◽  
Magnetic Measurements ◽  
Oxygen Deficiency ◽  
Perovskite Oxide ◽  
Ferromagnetic Behavior ◽  
Photoluminescence Intensity ◽  
Spin Ordering ◽  
Light Region ◽  
Green Fluorescent

Abstract Ferromagnetic semiconductors with luminescent effects provide a unique platform for studying magneto-electric-optical multifunctional devices. However, little is known about such materials with spin ordering well above room temperature. By using a unique high-pressure annealing method, a Cr and Fe disordered perovskite oxide SrCr0.5Fe0.5O2.875 (SCFO) with a simple cubic structure was prepared. Magnetic measurements demonstrated the ferromagnetic behavior with a spin ordering temperature as high as 600 K. In contrast to metallic SrCrO3 and SrFeO3, SCFO, with a moderate oxygen deficiency, is a direct bandgap semiconductor with an energy gap of 2.28 eV, which is within the visible light region. As a consequence, SCFO displays a green fluorescent effect arising from the d–p bonding and anti-bonding states. Moreover, the photoluminescence intensity can be tuned by a magnetic field. This work opens up a new avenue for research on room-temperature multifunctional materials with coupled magnetic, electrical, and optical performance.

scholarly journals Visualization of lymphatic vessels by Prox1-promoter directed GFP reporter in a bacterial artificial chromosome-based transgenic mouse

Blood ◽  
2011 ◽  
Vol 117 (1) ◽  
pp. 362-365 ◽  
Author(s):  
Inho Choi ◽  
Hee Kyoung Chung ◽  
Swapnika Ramu ◽  
Ha Neul Lee ◽  
Kyu Eui Kim ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mouse ◽  
Fluorescent Protein ◽  
Lymphatic Vessels ◽  
Artificial Chromosome ◽  
Transgenic Animal ◽  
Lymphatic Development ◽  
Vasculogenesis And Angiogenesis ◽  
Green Fluorescent

Abstract Although the blood vessel-specific fluorescent transgenic mouse has been an excellent tool to study vasculogenesis and angiogenesis, a lymphatic-specific fluorescent mouse model has not been established to date. Here we report a transgenic animal model that expresses the green fluorescent protein under the promoter of Prox1, a master control gene in lymphatic development. Generated using an approximately 200-kb-long bacterial artificial chromosome harboring the entire Prox1 gene, this Prox1-green fluorescent protein mouse was found to faithfully recapitulate the expression pattern of the Prox1 gene in lymphatic endothelial cells and other Prox1-expressing organs, and enabled us to conveniently visualize detailed structure and morphology of lymphatic vessels and networks throughout development. Our data demonstrate that this novel transgenic mouse can be extremely useful for detection, imaging, and isolation of lymphatic vessels and monitoring wound-associated lymphangiogenesis. Together, this Prox1-green fluorescent protein transgenic mouse will be a great tool for the lymphatic research.

scholarly journals Dual Education: The Win-Win Model of Collaboration between Universities and Industry

2018 ◽  
Vol 8 (3) ◽  
pp. 145 ◽  
Author(s):  
Monika Pogatsnik
Keyword(s):  
Higher Education ◽  
Soft Skills ◽  
Training Model ◽  
Practical Training ◽  
Full Time ◽  
Academic Education ◽  
Education Students ◽  
Program Outcomes ◽  
Good Tool ◽  
Industry Environment

The purpose of this paper is to describe the new experiences of the dual training model in engineering education in Hungary. This new model has been introduced recently in the higher education and has become a focus of interest. This is a fa-vorable program for the students to experience the real industry environment pri-or to graduation and it is a good tool to motivate them to study harder. The dual education students study in the institutional academic period together with the regular full-time students at their higher education institute, and parallel to their academic education they participate in the practical training. It gives the students an opportunity to join a specific training program at an enterprise. Being involved in specific "operational" practical tasks and project-oriented work enhances inde-pendent work, learning soft skills and experiencing the culture of work. Our ob-jectives are to analyze the benefits of the dual training for all three parties: the stu-dent, the company and university. The study confirms earlier results from prior studies which show, for example, that students who choose the dual option achieve better program outcomes.

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