Inhibitory Effects of Alendronate on Adhesion and Viability of Preosteoblast Cells on Titanium Discs

Abstract Objective The study aimed to investigate the effects of alendronate (ALN; a bisphosphonate) on adhesion and viability of preosteoblasts using different cell passages on sandblasted and acid-etched (SLA) Ti surfaces. Materials and Methods Preosteoblast, MC3T3, cells (passage 42; P42 and passage 62; P62) were cultured with ALN (1 and 5 µM) on cell culture plate for 7 days. Cells were lifted, counted, and seeded on SLA Ti surfaces. Cells were incubated on the discs for 6 hours to examine cell adhesion by using confocal microscopy and for 24 hours to determine cell viability by using MTT assay. Results ALN interfered with cell adhesion on Ti surfaces by reducing the cell number in both cell passages. Nuclei of untreated cells showed oval shape, whereas some nuclei of ALN-treated cells demonstrated crescent and condensed appearance. ALN at 1 and 5 µM significantly decreased nuclear area and perimeter in P42, while ALN at 5 µM reduced nuclear area and perimeter in P62. After 24 hours, cells (P42) grown on Ti surfaces showed decreased cell viability when culturing with 5 µM ALN. Conclusion ALN reduced cell adhesion and viability of preosteoblasts on Ti surfaces. ALN treatment seemed to exert higher inhibitory effects on nuclear shape and size as well as cell viability in lower cell passage. This led to the reduction in cell to implant surface interaction after encountering bisphosphonate treatment.

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The influence of laser frequency and groove distance on cell adhesion, cell viability, and antibacterial characteristics of Ti-6Al-4V dental implants treated by modern fiber engraving laser

Dental Materials ◽

10.1016/j.dental.2020.12.007 ◽

2021 ◽

Vol 37 (3) ◽

pp. 547-558

Author(s):

Neshat Eghbali ◽

Homam Naffakh-Moosavy ◽

Sanam Sadeghi Mohammadi ◽

Hossein Naderi-Manesh

Keyword(s):

Cell Adhesion ◽

Cell Viability ◽

Dental Implants ◽

Laser Frequency

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Effect of Different Titanium Dental Implant Surfaces on Human Adipose Mesenchymal Stem Cell Behavior. An In Vitro Comparative Study

Applied Sciences ◽

10.3390/app11146353 ◽

2021 ◽

Vol 11 (14) ◽

pp. 6353

Author(s):

Vittoria D’Esposito ◽

Josè Camilla Sammartino ◽

Pietro Formisano ◽

Alessia Parascandolo ◽

Domenico Liguoro ◽

...

Keyword(s):

Cell Viability ◽

Dental Implant ◽

In Vivo Studies ◽

Implant Surface ◽

Cytokines And Chemokines ◽

Titanium Dental Implant ◽

Adhesive Ability ◽

Stimulating Factor

Background: The aim of this research was to evaluate the effects of three different titanium (Ti) implant surfaces on the viability and secretory functions of mesenchymal stem cells isolated from a Bichat fat pad (BFP-MSCs). Methods: Four different Ti disks were used as substrate: (I) D1: smooth Ti, as control; (II) D2: chemically etched, resembling the Kontact S surface; (III) D3: sandblasted, resembling the Kontact surface; (IV) D4: blasted/etched, resembling the Kontact N surface. BFP-MSCs were plated on Ti disks for 72 h. Cell viability, adhesion on disks and release of a panel of cytokines, chemokines and growth factor were evaluated. Results: BFP-MSCs plated in wells with Ti surface showed a viability rate (~90%) and proliferative rate comparable to cells plated without disks and to cells plated on D1 disks. D2 and D4 showed the highest adhesive ability. All the Ti surfaces did not interfere with the release of cytokines, chemokines and growth factors by BFP-MSCs. However, BFP-MSCs cultured on D4 surface released a significantly higher amount of Granulocyte Colony-Stimulating Factor (G-CSF) compared either to cells plated without disks and to cells plated on D1 and D2. Conclusions: The implant surfaces examined do not impair the BFP-MSCs cell viability and preserve their secretion of cytokines and chemokines. Further in vitro and in vivo studies are necessary to define the implant surface parameters able to assure the chemokines’ optimal release for a real improvement of dental implant osseointegration.

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Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.8.165 ◽

2017 ◽

Vol 8 ◽

pp. 1649-1657 ◽

Cited By ~ 3

Author(s):

Antonín Brož ◽

Lucie Bačáková ◽

Pavla Štenclová ◽

Alexander Kromka ◽

Štěpán Potocký

Keyword(s):

Cell Viability ◽

Phase Contrast ◽

Cell Number ◽

Rapid Reduction ◽

Diamond Nanoparticles ◽

Cultivation Period ◽

High Pressure High Temperature ◽

Accessible Surface ◽

In Vitro Interactions

Diamond nanoparticles, known as nanodiamonds (NDs), possess several medically significant properties. Having a tailorable and easily accessible surface gives them great potential for use in sensing and imaging applications and as a component of cell growth scaffolds. In this work we investigate in vitro interactions of human osteoblast-like SAOS-2 cells with four different groups of NDs, namely high-pressure high-temperature (HPHT) NDs (diameter 18–210 nm, oxygen-terminated), photoluminescent HPHT NDs (diameter 40 nm, oxygen-terminated), detonation NDs (diameter 5 nm, H-terminated), and the same detonation NDs further oxidized by annealing at 450 °C. The influence of the NDs on cell viability and cell count was measured by the mitochondrial metabolic activity test and by counting cells with stained nuclei. The interaction of NDs with cells was monitored by phase contrast live-cell imaging in real time. For both types of oxygen-terminated HPHT NDs, the cell viability and the cell number remained almost the same for concentrations up to 100 µg/mL within the whole range of ND diameters tested. The uptake of hydrogen-terminated detonation NDs caused the viability and the cell number to decrease by 80–85%. The oxidation of the NDs hindered the decrease, but on day 7, a further decrease was observed. While the O-terminated NDs showed mechanical obstruction of cells by agglomerates preventing cell adhesion, migration and division, the H-terminated detonation NDs exhibited rapid penetration into the cells from the beginning of the cultivation period, and also rapid cell congestion and a rapid reduction in viability. These findings are discussed with reference to relevant properties of NDs such as surface chemical bonds, zeta potential and nanoparticle types.

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cAMP inhibition of interleukin-1-induced interleukin-6 production by human lung fibroblasts

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1993.264.3.l253 ◽

1993 ◽

Vol 264 (3) ◽

pp. L253-L260 ◽

Cited By ~ 3

Author(s):

R. J. Zitnik ◽

T. Zheng ◽

J. A. Elias

Keyword(s):

Human Lung ◽

Interleukin 1 ◽

Cell Number ◽

Lung Fibroblasts ◽

Intracellular Camp ◽

Inhibitory Effects ◽

Mrna Accumulation ◽

Human Lung Fibroblasts ◽

Dose Dependent ◽

Necrosis Factor

We characterized the effects of agents that alter intracellular adenosine 3',5'-cyclic monophosphate (cAMP) on the interleukin (IL)-6 production of human lung fibroblasts. Unstimulated fibroblasts did not produce significant amounts of IL-6. Recombinant (r) tumor necrosis factor (TNF) weakly stimulated, recombinant interleukin-1-alpha (rIL-1 alpha) strongly stimulated, and rIL-1 alpha and rTNF in combination synergistically augmented fibroblast IL-6 production. Prostaglandin (PG)E1, forskolin, dibutyryl cAMP (DBcAMP), 3-isobutyl-1-methylxanthine (IBMX), and cholera toxin did not cause a detectable alteration in the IL-6 production of unstimulated fibroblasts. However, these agents inhibited the IL-6 production of rIL-1 and rIL-1 plus rTNF-stimulated cells. These effects were dose dependent with a concentration of 2 x 10(-9) M PGE1, 5 x 10(-6) M forskolin, 5 x 10(-4) M DBcAMP, and 1 x 10(-3) M IBMX decreasing rIL-1 alpha (2.5 ng/ml)-induced IL-6 production by approximately 50%. The inhibitory effects of these agents, correlated with their ability to induce fibroblast cAMP accumulation, could not be explained by alterations in cell number or viability and were appreciable even when cAMP modifiers were added to fibroblast culture, 1 h after rIL-1. They were also at least partly specific for rIL-1, since these agents increased the IL-6 production of rTNF-stimulated cells. These cAMP-induced alterations in IL-6 production were associated with corresponding alterations in IL-6 mRNA accumulation. Nuclear run-on analysis demonstrated that the inhibitory effects of PGE1 were associated with a comparable decrease in IL-6 transcription. Agents that increase the levels of intracellular cAMP inhibit rIL-1-induced IL-6 by human lung fibroblasts.

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The neural cell adhesion molecule is necessary for normal adult retinal ganglion cell number and survival

Molecular and Cellular Neuroscience ◽

10.1016/j.mcn.2007.07.006 ◽

2007 ◽

Vol 36 (2) ◽

pp. 280-292 ◽

Cited By ~ 19

Author(s):

J.A. Murphy ◽

T.B. Franklin ◽

V.F. Rafuse ◽

D.B. Clarke

Keyword(s):

Cell Adhesion ◽

Ganglion Cell ◽

Retinal Ganglion Cell ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Neural Cell Adhesion Molecule ◽

Cell Number ◽

Neural Cell ◽

Retinal Ganglion ◽

Neural Cell Adhesion

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Inhibitory effects of Panax ginseng glycoproteins in models of doxorubicin-induced cardiac toxicity in vivo and in vitro

Food & Function ◽

10.1039/d1fo01307f ◽

2021 ◽

Author(s):

Yajun Chen ◽

Lei Wang ◽

Tianjia Liu ◽

Zhidong Qiu ◽

Ye Qiu ◽

...

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Cell Viability ◽

Panax Ginseng ◽

Cardiac Toxicity ◽

H9c2 Cell ◽

Inhibitory Effects ◽

Myocardial Oxidative Stress

We investigated the protective effect of PGP against DOX-induced cardiotoxicity in vitro and in vivo. PGP increases H9C2 cell viability and inhibits apoptosis, alleviating DOX-induced myocardial oxidative stress-related cardiotoxicity.

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Surface Modification of PLLA, PTFE and PVDF with Extreme Ultraviolet (EUV) to Enhance Cell Adhesion

International Journal of Molecular Sciences ◽

10.3390/ijms21249679 ◽

2020 ◽

Vol 21 (24) ◽

pp. 9679

Author(s):

Adam Lech ◽

Beata A. Butruk-Raszeja ◽

Tomasz Ciach ◽

Krystyna Lawniczak-Jablonska ◽

Piotr Kuzmiuk ◽

...

Keyword(s):

Surface Modification ◽

Cell Adhesion ◽

Endothelial Cell ◽

Vinylidene Fluoride ◽

Extreme Ultraviolet ◽

Cell Number ◽

Laboratory System ◽

Poly Vinylidene Fluoride ◽

The Impact ◽

Endothelial Cell Adhesion

Recently, extreme ultraviolet (EUV) radiation has been increasingly used to modify polymers. Properties such as the extremely short absorption lengths in polymers and the very strong interaction of EUV photons with materials may play a key role in achieving new biomaterials. The purpose of the study was to examine the impact of EUV radiation on cell adhesion to the surface of modified polymers that are widely used in medicine: poly(tetrafluoroethylene) (PTFE), poly (vinylidene fluoride) (PVDF), and poly-L-(lactic acid) (PLLA). After EUV surface modification, which has been performed using a home-made laboratory system, changes in surface wettability, morphology, chemical composition and cell adhesion polymers were analyzed. For each of the three polymers, the EUV radiation differently effects the process of endothelial cell adhesion, dependent of the parameters applied in the modification process. In the case of PVDF and PTFE, higher cell number and cellular coverage were obtained after EUV radiation with oxygen. In the case of PLLA, better results were obtained for EUV modification with nitrogen. For all three polymers tested, significant improvements in endothelial cell adhesion after EUV modification have been demonstrated.

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