Effects of dietary probiotics on growth performance, faecal microbiota and serum profiles in weaned piglets

2014 ◽  
Vol 54 (5) ◽  
pp. 616 ◽  
Author(s):  
Xiaoli Dong ◽  
Naifeng Zhang ◽  
Meng Zhou ◽  
Yan Tu ◽  
Kaidong Deng ◽  
...  
Keyword(s):  
Growth Performance ◽  
Early Stage ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Feed Conversion ◽  
Faecal Microbiota ◽  
Weaned Piglets ◽  
Serum Globulin ◽  
Large White ◽  
E Coli

This study was conducted to evaluate the effects of dietary supplementation with probiotics on growth performance, faecal microbiota, and serum profiles in weaned piglets. Large White × Landrace piglets (n = 144) weaned at 35–37 days of age were selected and divided into four groups, and the piglets from each group were assigned randomly to six pens (replicates) with six animals each. Each group was fed one of four diets for 5 weeks: a basal diet without antibiotics and probiotics (control), or the basal diet supplemented with Lactobacillus plantarum GF103, Bacillus subtilis B27, or a mixture of L. plantarum GF103 and B. subtilis B27. During the first 2 weeks of the supplementation, the piglets supplemented with probiotics had lower (P < 0.05) average daily feed intake than control piglets. The feed conversion ratio was improved (P < 0.05) in probiotic-supplemented groups compared with that of control. The population of E. coli in faeces of the piglets supplemented with L. plantarum GF103 was lower (P < 0.05) than that of control piglets. On day 14, dietary supplementation of the combination of L. plantarum GF103 and B. subtilis B27 increased (P < 0.05) the serum concentrations of total protein, globulin, and creatinine, but decreased (P < 0.05) the ratio of serum albumin to serum globulin, compared with the basal diet. On day 14, dietary supplementation with probiotics increased (P < 0.05) the serum IgM concentration compared with the basal diet. Supplementation of B. subtilis B27 or the combination of L. plantarum GF103 and B. subtilis B27 increased (P < 0.05) the serum IgA concentration at the end of the trial. These results suggest that dietary probiotics improved growth performance and enhanced immune responses at the early stage of the post-weaning period in piglets.

scholarly journals The involvement of NF-κB/P38 pathways inScutellaria baicalensisextracts attenuating ofEscherichia coliK88-induced acute intestinal injury in weaned piglets

2019 ◽  
Vol 122 (2) ◽  
pp. 152-161 ◽  
Author(s):  
Caiyun Huang ◽  
Yubo Wang ◽  
Xin He ◽  
Ning Jiao ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Dietary Supplementation ◽  
Basal Diet ◽  
Signalling Pathways ◽  
Feed Conversion ◽  
Weaned Piglets ◽  
E Coli ◽  
Colony Forming Units ◽  
Villous Height ◽  
Weaning Piglets ◽  
To Receive

AbstractThe present study was carried out to evaluate the effect of dietary supplementation ofScutellaria baicalensisextracts (SBE) on intestinal health in terms of morphology, barrier integrity and immune responses in weaned piglets challenged withEscherichia coliK88. A total of seventy-two weaned piglets were assigned into two groups to receive a basal diet without including antibiotic additives or the basal diet supplemented 1000 mg SBE/kg diet for 14 d. On day 15, twelve healthy piglets from each group were selected to expose to oral administration of either 10 ml 1 × 109colony-forming units ofE. coliK88 or the vehicle control. After 48 h ofE.coliK88 challenge, blood was sampled, and then all piglets were killed humanely for harvesting jejunal and ileal samples. Dietary supplementation of SBE significantly decreased diarrhoea frequency and improved feed conversion ratio (P< 0·05). SBE supplementation toE.coliK88-challenged piglets improved villous height and villous height/crypt depth (P< 0·05), recovered the protein expression of occludin and zonula occludens-2 in both the jejunum and ileum (P< 0·05), and mitigated the increases in plasma IL-1β, TNF-α, IL-6, IgA and IgG (P< 0·05). Meanwhile, dietary SBE effectively inhibited the stimulation of NF-κB, P38 and TNF-αas well as IL-1βin the small intestine of piglets challenged byE. coliK88 and prevented the activation of NF-κB/P38 signalling pathways (P< 0·05). Collectively, SBE supplementation can potently attenuate diarrhoea in weaning piglets and decrease inflammatory cytokine expressions through inhibiting the NF-κB and P38 signalling pathways.

Effects of dietary Korean garlic extract aged by Leuconostoc mesenteroides KCCM35046 on growth performance, digestibility, blood profiles, gas emissions, and microbiota in weanling pigs

2020 ◽  
Vol 100 (3) ◽  
pp. 462-469
Author(s):  
J.H. Cho ◽  
S.D. Liu ◽  
I.H. Kim
Keyword(s):  
Growth Performance ◽  
Average Daily Gain ◽  
Dietary Supplementation ◽  
Experimental Unit ◽  
Feed Conversion ◽  
Weanling Pigs ◽  
Gas Emissions ◽  
E Coli ◽  
Gross Energy ◽  
Dietary Treatments

A total of 150 crossbred pigs [(Duroc × Yorkshire) × Landrace; body weight (BW) = 5.74 ± 1.15 kg; weaned at day 21 after birth] were assigned to one of five dietary treatments (six pens per treatment and five pigs per pen) by BW and sex (two barrows and three gilts) for a 6 wk experiment. The pen was considered the experimental unit in the statistical model. Treatments were corn–soybean meal diets supplemented with 0.0033% tiamulin as well as 0%, 0.05%, 0.10%, or 0.20% fermented garlic (FG). The average daily gain, average daily feed intake (ADFI), and feed conversion ratio were linearly (p < 0.05) increased by FG inclusion during days 21–42 and days 0–42. Dietary supplementation of FG linearly increased (p < 0.05) the coefficient of apparent total tract digestibility of dry matter, nitrogen, and gross energy as well as blood immunoglobulin G concentration during week 6. The fecal ammonia (NH3), total mercaptan emissions, and Escherichia coli counts were linearly (p < 0.05) reduced by FG in the diet. Dietary supplementation of FG increased (p < 0.05) ADFI during days 21–42 and days 0–42 and reduced (p < 0.05) fecal NH3 emissions compared with pigs fed tiamulin. In conclusion, the results indicate that FG can enhance growth performance and digestibility, and reduce fecal E. coli counts as well as fecal gas emissions in weanling pigs.

scholarly journals Effects of Dietary Zinc Sources on Growth Performance and Gut Health of Weaned Piglets

2021 ◽  
Vol 12 ◽  
Author(s):  
Hui Diao ◽  
Jiayou Yan ◽  
Shuwei Li ◽  
Shengyao Kuang ◽  
Xiaolan Wei ◽  
...  
Keyword(s):  
Growth Performance ◽  
Mrna Expression ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Expression Level ◽  
Control Group ◽  
Mrna Expression Level ◽  
Gut Health ◽  
Weaned Piglets ◽  
Zinc Sources

The present study aimed to investigate the effects of dietary zinc sources on the growth performance and gut health of weaned piglets. In total, 96 Duroc × Landrace × Yorkshire (DLY) weaned piglets with an initial average body weight of 8.81±0.42kg were divided into four groups, with six replicates per treatment and four pigs per replicate. The dietary treatment groups were as follows: (1) control group, basal diet; (2) zinc sulphate (ZnSO4) group, basal diet +100mg/kg ZnSO4; (3) glycine zinc (Gly-Zn) group, basal diet +100mg/kg Gly-Zn and (4) zinc lactate group, and basal diet +100mg/kg zinc lactate. The whole trial lasted for 28days. Decreased F/G was noted in the Gly-Zn and zinc lactate groups (p&lt;0.05). The zinc lactate group had a lower diarrhea rate than the control group (p&lt;0.05). Moreover, the ZnSO4, Gly-Zn, and zinc lactate groups had significantly higher apparent total tract digestibility of dry matter (DM), crude protein (CP), ether extract (EE), crude ash, and zinc than the control group (p&lt;0.05). The Gly-Zn and zinc lactate groups had higher jejunal villus height and a higher villus height:crypt depth ratio than the control group (p&lt;0.05). In addition, the ZnSO4, Gly-Zn and zinc lactate groups had a significantly lower mRNA expression level of jejunal ZRT/IRT-like protein 4 (ZIP4) and higher mRNA expression level of jejunal interleukin-1β (IL-1β) than the control group (p&lt;0.05). The mRNA expression level of jejunal zinc transporter 2 (ZNT2) was higher and that of jejunal Bcl-2-associated X protein (Bax) was lower in the Gly-Zn and zinc lactate groups than in the control group (p&lt;0.05). Moreover, the zinc lactate group had a higher count of Lactobacillus spp. in the cecal digesta and higher mRNA expression levels of jejunal occludin and mucin 2 (MUC2) than the control group (p&lt;0.05). In conclusion, dietary supplementation with 100mg/kg ZnSO4, Gly-Zn, or zinc lactate could improve the growth performance and gut barrier function of weaned piglets. Dietary supplementation with organic zinc, particularly zinc lactate, had the best effect.

scholarly journals PSVI-40 Effects of dietary glucan on intestinal morphology, immunity response, barrier function and antioxidant capacity in weaning pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 212-213
Author(s):  
xia xiong ◽  
Lvliang Wu ◽  
Yirui Shao ◽  
Jian zou ◽  
Yulong Yin
Keyword(s):  
Antioxidant Capacity ◽  
Growth Performance ◽  
Mrna Expression ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Gut Health ◽  
Weaned Piglets ◽  
Weaning Piglets

Abstract Glucan has been studied as a potential alternative to antibiotics for animals in recent years. The aim of this study was to evaluate the effect of dietary glucan on growth performance and gut health of weaning piglets, which is a water-soluble extracellular ꞵ-glucan produced by Agrobacterium sp. ZX09. A total of 108 weaned piglets (21 d of age; 6.05 ± 0.36 kg) were randomly assigned (6 pens/diet; 18 piglets/pen) to 3 dietary treatments consisting of a basal diet (control group) or the basal diet supplemented with 20 ppm olaquindox or 200 ppm glucan for 14 days, respectively. The results showed that piglets fed with glucan had greater (P &lt; 0.05) body weight and average daily gain than piglets in control group. Piglets fed with glucan or antibiotic had greater villus height to crypt depth ratio on duodenum compared with control group (P &lt; 0.05). The mRNA expression of Claudin-1 on duodenum or ileum was higher (P &lt; 0.05) in glucan group than that on the other groups. The mRNA expression of TLR4, MYD88 and NFκB on jejunum were lower (P &lt; 0.05) in glucan or antibiotic group than those in control group. Dietary supplementation with glucan tended to increase the IL-10 and SIgA concentration on ileum (0.05 &lt; P &lt; 0.1). Dietary supplementation with glucan tended to increase the total antioxidant capacity on jejunum (P = 0.093). In conclusion, 200 ppm glucan or 20 ppm olaquindox can improve the growth performance of weaning piglets. The glucan may can accelerate the growth of weaned piglets by improving gut health. This research will provide guidance for the olaquindox alternative on growing piglets.

scholarly journals Effects of Dietary Supplementation with Combination of Tributyrin and Essential Oil on Gut Health and Microbiota of Weaned Piglets

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 180 ◽  
Author(s):  
Wen-Xin Zhang ◽  
Yu Zhang ◽  
Xiao-Wei Zhang ◽  
Zhao-Xi Deng ◽  
Jian-Xin Liu ◽  
...  
Keyword(s):  
Methyl Salicylate ◽  
Animal Health ◽  
Morphological Structure ◽  
Basal Diet ◽  
Gut Health ◽  
Feed Conversion ◽  
Weaned Piglets ◽  
Villus Height ◽  
Large White ◽  
The Difference

The aim of this study was to determine the effects of dietary inclusion of the combination of tributyrin with oregano or methyl salicylate as a substitute to antibiotics on gut health and microbiota of piglets. A total of 48 weaned crossbred piglets (Duroc × Large White × Landrace, 8.79 ± 0.97 kg, 21 ± 1 d) were randomly allocated to four experimental groups and fed for 4 weeks: the basal diet (Con); the control plus antibiotics (AB); the control plus oregano and tributyrin (OT); and the control plus methyl salicylate and tributyrin (MT). Although a numerical improvement on feed intake, weight gain and feed conversion ratio was observed in the OT and MT as well as the AB group, the difference was not significant (p > 0.05). The OT and MT groups were larger in villus height in the duodenum compared to the Con (p < 0.05), and were larger in relative abundance of Firmicutes/Bacaeroides in the intestine compared to Con and AB groups (p < 0.01). The amount of major different metabolites was 6, 8 and 8 for the AB, OT and MT groups when compared to the Con, respectively. In conclusion, as a substitute for antibiotics the inclusion of the combination of tributyrin with either oregano or methyl salicylate to the diet of weaned piglets improved the intestinal morphological structure and altered intestinal microbiota and metabolites, which were beneficial to the animal health.

scholarly journals Effects of dietary supplementation with exogenous catalase on growth performance, oxidative stress, and hepatic apoptosis in weaned piglets challenged with lipopolysaccharide

2020 ◽  
Vol 98 (3) ◽  
Author(s):  
Yang Li ◽  
Xilun Zhao ◽  
Xuemei Jiang ◽  
Ling Chen ◽  
Liang Hong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Growth Performance ◽  
Mrna Expression ◽  
Rectal Temperature ◽  
Caspase 3 ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Weaned Piglets ◽  
Post Challenge ◽  
Blood Samples

Abstract Two experiments were conducted to investigate the effects of exogenous catalase (CAT) in the diet of weaned piglets on growth performance, oxidative capacity, and hepatic apoptosis after challenge with lipopolysaccharide (LPS). In experiment 1, 72 weaned piglets [Duroc × Landrace × Yorkshire, 6.90 ± 0.01 kg body weight (BW), 21 d of age] were randomly assigned to be fed either a basal diet (CON group) or a basal diet supplemented with 2,000 mg/kg CAT (CAT group; dietary CAT activity, 120 U/kg) for 35 d. Blood samples were collected on day 21 and day 35. At the end of this experiment, 12 pigs were selected from each of the CON and CAT groups, and six pigs were injected with LPS (50 μg/kg BW), while the remaining six pigs were injected with an equal amount of sterile saline, resulting in a 2 × 2 factorial arrangement of treatments (experiment 2). Blood samples and rectal temperature data were collected 0 and 4 h after challenge, and liver samples were obtained after evisceration. The gain-to-feed ratio was higher (P &lt; 0.05) in piglets in the CAT group than in those in the CON group from day 1 to 35. Catalase and total superoxide dismutase (T-SOD) activities were higher (P &lt; 0.05), whereas malondialdehyde (MDA) concentrations were lower (P &lt; 0.05), in piglets in the CAT group than in those in the CON group at day 35. During challenge, rectal temperature and liver MDA and H2O2 concentrations increased significantly (P &lt; 0.05), whereas plasma CAT and glutathione peroxidase (GSH-Px) activities and liver CAT activity decreased markedly (P &lt; 0.05), in LPS-challenged piglets 4 h post-challenge. Increased CAT activity and decreased MDA concentration were observed in the plasma and liver of piglets in the CAT group 4 h post-challenge (P &lt; 0.05). Dietary CAT supplementation markedly suppressed the LPS-induced decrease in plasma GSH-Px activity and liver CAT activity to levels observed in the CON group (P &lt; 0.05) as well as significantly decreasing the concentration and mRNA expression of caspase-3 and caspase-9 (P &lt; 0.05). LPS-induced liver injury was also attenuated by dietary CAT supplementation, as demonstrated by a decrease in liver caspase-3 mRNA expression (P &lt; 0.05). Overall, dietary supplementation with 2,000 mg/kg exogenous CAT (dietary CAT activity, 120 U/kg) improves growth performance and has a beneficial effect on antioxidant capacity in weaned piglets; alleviates oxidative stress and reduces liver damage by suppressing hepatic apoptosis in LPS-challenged piglets.

scholarly journals Effects of fermented bamboo vinegar liquid on growth performance, nutrient digestibility, faecal Escherichia coli concentration and ammonia emissions in growing pigs

1970 ◽  
Vol 48 (4) ◽  
pp. 621-626
Author(s):  
S.T. Ahmed ◽  
H-S. Mun ◽  
S-B. Son ◽  
C-J. Yang
Keyword(s):  
Escherichia Coli ◽  
Growth Performance ◽  
Dietary Supplementation ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Daily Weight Gain ◽  
Control Group ◽  
Ammonia Emissions ◽  
Feed Conversion ◽  
E Coli

Fermented bamboo vinegar liquid (FBVL) was prepared through liquid fermentation of bamboo vinegar with multispecies probiotics, and its effect on growing pigs was investigated. A 42-day feeding trial with 84 growing pigs (28.0 ± 0.08 kg) was conducted to evaluate the effects of FBVL on growth performance, nutrient digestibility, faecal Escherichia coli concentration and faecal ammonia emissions. Pigs were randomly distributed to one of four dietary treatments, namely control, CTC (0.003% chlortetracycline), FBVL 0.2%, and FBVL 0.4%. Overall, the final weight and daily weight gain of the pigs in the CTC group were higher than that of pigs in the control group. Dietary supplementation with 0.4% FBVL reduced the feed intake of growing pigs, whereas the feed conversion ratio (FCR) was lower in the CTC supplemented group. The dry matter digestibility was higher in pigs fed CTC and 0.2% FBVL diets, and crude protein digestibility was improved in all treatment groups compared with control. The faecal E. coli numbers were reduced in response to CTC and 0.4% FBVL supplementation. Faecal ammonia emissions were reduced by dietary supplementation with CTC and FBVL at weeks 1, 2 and 3. In conclusion, dietary FBVL supplementation increased nutrient digestibility and reduced faecal E. coli population and ammonia emissions without negative effects on growth performance.Keywords: Chlortetracycline, digestibility, fermentation, probiotics, swine

scholarly journals Mixture of Five Fermented Herbs (Zhihuasi Tk) Alters the Intestinal Microbiota and Promotes the Growth Performance in Piglets

2021 ◽  
Vol 12 ◽  
Author(s):  
Yong Li ◽  
Tiehu Sun ◽  
Yuxuan Hong ◽  
Tong Qiao ◽  
Yongsheng Wang ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Microbiota ◽  
Pathogenic Bacteria ◽  
Dietary Supplementation ◽  
Feed Additives ◽  
Epithelial Cell Line ◽  
Taxonomic Structure ◽  
Feed Conversion ◽  
Weaned Piglets

To explore the feasibility of using fermented Chinese herbal mixture Zhihuasi Tk (Z. Tk) supplementation to increase the swine production, the protective effect of dietary supplementation with Z. Tk on the intestinal oxidative stress model and the regulation of both growth performance and intestinal microbiota of weaned piglets were investigated in vitro. Our results showed that the addition of Z. Tk increased the cell viability, prevented the decrease of glutathione peroxidase, and significantly increased the total antioxidant capacity and reduced the damage caused by H2O2 to the tight junction proteins of the porcine small intestinal epithelial cell line (IPEC-J2). Furthermore, weaned piglets supplemented with either 2 kg/ton zinc oxide (ZnO) or 4 kg/ton of Z. Tk in the diet increased body weight as well as average daily feed intake and daily gain, while the feed conversion rate and diarrhea rate decreased within 0–35 days. Results of the taxonomic structure of the intestinal microbiota showed that, in 21 days after weaning, the Firmicutes/Bacteroidetes ratio in experimental group was increased, while the abundance of beneficial bacteria such, as Lactobacillus, was increased by Z. Tk, showing inhibitory effect on pathogenic bacteria such as members of Proteobacteria. In summary, dietary supplementation with Z. Tk maintained the intestinal microbiota in a favorable state for the host to effectively reduce the abnormal changes in the intestinal microbial structure and improved growth performance of weaned piglets. Therefore, Z. Tk may potentially function as a substitute for ZnO in feed additives for weaned piglets in modern husbandry.

scholarly journals Effect of dietary supplementation of neem oil (Azadirachtaindica) on the growth performance and nutrient digestibility of weaned rabbits

2020 ◽  
Vol 3 (10) ◽  
pp. 202-209
Author(s):  
Oluwafemi R. A ◽  
Oluwayinka E. O ◽  
Alagbe J. O
Keyword(s):  
Weight Gain ◽  
Growth Performance ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Average Weight ◽  
Neem Oil ◽  
Feed Conversion ◽  
Crude Fibre ◽  
Randomized Design

The objective of the present study was to determine the effect of dietary supplementation of neem oil (Azadirachtaindica) on the growth performance and nutrient digestibility of weaned rabbits. A total of 50 weaned male cross bred rabbits between 5-6 weeks with an average weight of 565.4g-566.8gwere divided into five dietary groups of ten (10) weaned rabbits each in a completely randomized design. The dietary treatments include a control, T1 (basal) diet with no neem oil (NOL), T2, T3, T4 and T5 were fed basal diet supplemented with NOL at 0.1%, 0.2%, 0.3% and 0.4% respectively. Feed and water were offered ad libitum throughout the experiment which lasted for 12 weeks. The data obtained was used to evaluate the growth performance: weight gain (WG), feed intake (FI), feed conversion ratio (FCR), mortality and nutrient digestibility (dry matter, crude protein, crude fibre and ether extract). WG, ADFI, TFI were significantly (P˂0.05) different among the treatments. T5 had the highest weight gain (755.90 g) followed by T4(734.0g), T3(705.90g), T2(705.0g) and T1(621.80g) respectively. Highest mortality was recorded in T1 (2%) followed by T2 (1%), none was recorded in T3, T4 and T5. Neem oil significantly influenced (P˂0.05) all the parameters measured. It could be concluded thatneem oil contains some essential nutrients and bioactive chemicals and could be supplemented in the diet of rabbit at 0.4 % without any deleterious effect on the general performance of the animal.

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