Use of different carbohydrate sources associated with urea and implications for in vitro fermentation and rumen microbial populations

2020 ◽  
Vol 60 (8) ◽  
pp. 1028 ◽  
Author(s):  
K. C. Santos ◽  
F. F. R. Carvalho ◽  
M. M. Carriero ◽  
A. L. R. Magalhães ◽  
A. M. V. Batista ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Short Chain Fatty Acids ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Protein Nitrogen ◽  
Microbial Profile ◽  
In Vitro Gas Production ◽  
Nellore Cattle ◽  
True Protein

Context Alternative feed sources have been investigated as replacements for green forages and cereal grains traditionally used in ruminant feed. We hypothesised that, when replacing sources of true protein with non-protein nitrogen (NPN) in the ruminant diet, the efficiency of utilisation of the NPN may be affected by the source of energy and that different energy resources used as alternatives to maize may improve efficiency and maximise ruminal fermentation characteristics. Aims The objective of this study was to evaluate the effects of diets containing different carbohydrate sources associated with urea on in vitro ruminal fermentation and rumen microbial profile. Methods Four diets based on Tifton 85 Bermuda grass hay (584 g/kg dry matter) were tested as substrates: cornmeal + soybean meal (C + SM, typical diet), cornmeal + urea (C + U), cassava scraping + urea (CS + U), and spineless cactus + urea (SC + U). The experimental design consisted of randomised blocks with four treatments and five blocks. Five adult Nellore cattle with permanent fistula in the rumen were used as inoculum donors. The semi-automatic in vitro gas production technique was used in two experiments. Quantitative real-time PCR was used to monitor the changes in the rumen microbial community. Key results The diets containing C + U and CS + U decreased (P < 0.05) concentrations of isobutyrate, isovalerate, and valerate after 24 h of incubation, and all diets containing urea decreased (P < 0.05) concentrations of isobutyrate, isovalerate and valerate after 96 h and increased (P < 0.05) acetate:propionate ratio. After 96 h of incubation, the diets containing CS + U and SC + U resulted in a lower (P < 0.05) population of Ruminococcus flavefaciens than the C + U diet, and a lower (P < 0.05) population of Streptococcus bovis than the C + SM diet. Conclusions From our results, a diet containing cassava scraping produces more methane per unit of degraded organic matter, which reduces fermentation efficiency. Diets that contain corn with either soybean meal or urea result in greater degradability with lower gas production rates than diets that contain either cassava scrapings or spineless cactus with urea. Diets containing urea as a total substitution for soybean meal alter the production of short-chain fatty acids and reduce the populations of S. bovis and R. flavefaciens. Implications Use of urea to replace soybean meal in the ruminant diet alters ruminal fermentation and rumen microbial population.

scholarly journals Effects of exogenous enzymes on in vitro gas production kinetics and ruminal fermentation of four fibrous feeds

2013 ◽  
Vol 179 (1-4) ◽  
pp. 46-53 ◽  
Author(s):  
M.M.Y. Elghandour ◽  
A.Z.M. Salem ◽  
M. Gonzalez-Ronquillo ◽  
J.L. Bórquez ◽  
H.M. Gado ◽  
...  
Keyword(s):  
Gas Production ◽  
Ruminal Fermentation ◽  
Production Kinetics ◽  
In Vitro Gas Production ◽  
Exogenous Enzymes

scholarly journals Kinetics in vitro of ruminal fermentation of cocoa husks subjected to alkali and heat treatment

2015 ◽  
Vol 36 (6) ◽  
pp. 3897
Author(s):  
Flávio Moreira de Almeida ◽  
José Augusto Gomes Azevêdo ◽  
Ícaro Dos Santos Cabral ◽  
Luiz Gustavo Ribeiro Pereira ◽  
Gherman Garcia Leal de Araújo ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Alkali Treatment ◽  
Alkaline Treatment ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Thermal Methods ◽  
Final Volume ◽  
In Vitro Gas Production ◽  
Efficient Alternative

The objective was to evaluate the parameters of kinetics of ruminal fermentation of cocoa husks (CH) treated with alkali and thermal agents, using the semi-automated in vitro gas production technique. Cocoa husks samples were subjected to alkali and thermal methods (effect of time of exposure) treatment, as follows: control; alkaline treatment with calcium hydroxide ((Ca(OH)2) and calcium oxide (CaO), both doses of 15.0; 30.0 and 45.0 g kg-1 of CH; heat treatment in an autoclave at a pressure of 1.23 kg cm-2 (15 psi) and a temperature of 123°C for 30, 60 and 90 minutes. For statistical analysis, orthogonal contrasts and regression. The degradation rate and the final volume of gases of non-fiber carbohydrates decreased with the addition of Ca(OH)2 and CaO, however, for fibrous carbohydrates effects were positive. For each percentage of Ca(OH)2 and CaO included, it is estimated an increase of 5.74 and 2.9% in the final volume of the fiber, respectively. When the heat treatment, a decrease in all parameters was estimated. For each minute of exposure to heat, there was a decrease of 0.4% in total final volume of gases. The alkali treatment can be an efficient alternative for improving the digestibility of fibrous fractions of CH.

In Vitro gas production assessement of Panicum maximum incubated with Leucaena leucocephala at varying proportions to predict the nutritional value for ruminants

2021 ◽  
Vol 44 (3) ◽  
pp. 371-377
Author(s):  
O. O. Falola ◽  
O. O. Olufayo
Keyword(s):  
Leucaena Leucocephala ◽  
Crude Protein ◽  
Small Ruminants ◽  
Short Chain Fatty Acids ◽  
Gas Production ◽  
Panicum Maximum ◽  
In Vitro Gas Production ◽  
Metabolisable Energy ◽  
The Tropics

The inclusion of multipurpose trees with grass such as Panicum maximum in the diet of ruminants may enhance productivity. Thus, the study was carried out to determine the proximate composition, in vitro gas production characteristics and parameters of Leucaena leucocephala and Panicum maximum at varying proportions. Five diets were formulated such that Panicum maximum was substituted with Leucaena leucocephala at different ratio: T1 (100% Panicum maximum), T2 (100% Leuceana leucocephala), T3 (50% P. maximum + 50% L. leucocephala), T4 (75% P. maximum + 25% L .leucocephala) and T5 (25% P. maximum + 75% L. leucocephala). Data were subjected to analysis of variance. Values obtained for dry matter (34.43 –35.95g/100g) decreased with the increased inclusion of Leuceana leucocephala in the diets. The crude fiber values (14.33 – 30.75g/100g) also followed the same trend while crude protein (CP) content (10.70 – 26.78g/100g) increased the inclusion of Leucaena leucocephala in the diets. There were significant (P < 0.05) differences in the treatment means of organic matter digestibility (OMD 31.93 – 37.07%), Metabolisable energy (ME 3.62 – 4.33 MJ/kgDM), short chain fatty acids (SCFA 0.04 – 0.16mL) and methane (ME 1.00 – 2.50 mL).The values (2.00 – 4.67 ml/200mgDM) obtained for immediate soluble 'a' was significantly (P < 0.05) different among the treatments. The extent of gas production (a+b) ranged from 2.00 – 4.67 mL/200mgDM, T1 (100% Panicum maximum) recorded the lowest while highest was observed in T2 (100% Leucaena leucocephala). The insoluble but degradable fraction 'b'ranged from 2.67 – 5.67ml/200mgDM, while the rate of gas production 'c' ranged from 0.04 – 0.14ml/hr. In conclusion, the enhanced values of crude protein, OMD, SCFA, and ME in the Panicum maximum and Leucaena leucocephala mixture indicate that the diets is able to meet the nutrients requirements of small ruminants in the tropics especially during the dry season.

Evaluation of nutritional value in some tropical by-products using the in vitro gas production technique

2009 ◽  
Vol 2009 ◽  
pp. 190-190
Author(s):  
A Taghizadeh ◽  
M Besharati
Keyword(s):  
Rumen Fluid ◽  
Short Chain Fatty Acids ◽  
Gas Production ◽  
Production Technique ◽  
Production Methods ◽  
Feeding Programs ◽  
Body Of Knowledge ◽  
In Vitro Gas Production ◽  
Digestion Kinetics

Anaerobic digestion of carbohydrates by ruminal microbes produces short chain fatty acids (SCFA), CO2, CH4, and traces of H2; hence, measurement of gas production in vitro can be used to study the rate and extent of digestion of feedstuffs (Hungate, 1966). When a feedstuff is incubated with buffered rumen fluid in vitro, the carbohydrates are fermented to SCFA, gases mainly CO2 and CH4 and microbial cells. Gas production is basically the result of fermentation of carbohydrates to acetate, propionate and butyrate (Wolin, 1960; Beuvink and Spoelstra, 1992; Blummel and Ørskov, 1993). High correlations between gas production and NDF disappearance, r2 = 0.99 (Pell and Schofield, 1993) or gas production and DM disappearance, r2 = 0.95 (Prasad et al., 1994) have been reported. In vitro techniques that estimate digestion kinetics indirectly by measuring gas production are a more viable option than other in vitro methods. Gas production technology allows for a more usable collection of digestion kinetics data and has allowed for a growing body of knowledge that is directly applicable to the feeding programs that are in daily practical field use. The range of data that can be acquired is broad and will no doubt grow over time. One of the most challenging problems associated with using gas production methods is that the amount of gas produced varies with different molar proportions of SCFA. For example, a higher propionate concentration is associated with lower gas production because an extra carbon atom in propionate would otherwise have appeared as CO2 (Wolin, 1960). The object of this study was to evaluate the nutritional quality of noodle waste (NW), tomato pomace (TP) and apple pomace (AP) using the gas production technique.

In vitro gas production and ruminal fermentation of glycerol, propylene glycol and molasses

2009 ◽  
Vol 154 (1-2) ◽  
pp. 112-118 ◽  
Author(s):  
S.M. Ferraro ◽  
G.D. Mendoza ◽  
L.A. Miranda ◽  
C.G. Gutiérrez
Keyword(s):  
Propylene Glycol ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
In Vitro Gas Production

scholarly journals In vitro ruminal fermentation kinetics of diets with crambe cake protein replacing soybean meal protein by gas production technique

2021 ◽  
Vol 42 (6) ◽  
pp. 3399-3414
Author(s):  
Angela Rocio Poveda-Parra ◽  
Odimári Pricila Prado-Calixto ◽  
Elzânia Sales Pereira ◽  
Fernando Luiz Massaro Junior ◽  
Larissa Nóbrega de Carvalho ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Dry Matter ◽  
Gas Production ◽  
Corn Silage ◽  
Ruminal Fermentation ◽  
Production Technique ◽  
Meal Protein ◽  
Feedlot Lambs ◽  
Gas Volume

The objective of this study was to evaluate ingredients and diets containing increasing levels of crambe cake protein replacing soybean meal protein, with in vitro ruminal fermentation parameters using a gas production technique. Diets were formulated for feedlot lambs and contained different levels of crambe cake protein (0, 250, 500, 750, and 1000 g kg-1) replacing soybean meal protein. Corn silage was used as roughage. Carbohydrate digestion rates were estimated using the in vitro gas production technique and the cumulative gas production kinetics were analyzed using the bicompartmental logistic model. The parameters values of ruminal degradation kinetics were generated using the R statistical program with the Gauss-Newton algorithm and then subjected to analysis of variance and regression (when necessary) according to a completely randomized experimental design with five treatments and five replications. Upon carbohydrate fractionation of ingredients and experimental diets, it was observed that corn grain and corn silage presented the highest levels of total carbohydrates (TC), with values of 128.3 and 464.8 g kg-1 dry matter (DM) in fraction B2, respectively. Lower TC content was found for soybean meal and crambe cake (CC). There was a predominance of fractions A + B1 in the ingredients and experimental diets. The B2 fraction decreased in the diets with the inclusion of the CC protein, and CC presented the highest C fraction. Protein fractionation (g kg-1 DM and g kg-1 crude protein - CP), the ingredients and diets showed a higher proportion of fractions A and B1 + B2. In in vitro degradation, the diet without CC (0 g kg-1 DM) showed the highest final cumulative gas production (365.04 mL g-1 of incubated DM), while the CC presented the lowest volume (166.68 mL g-1 of incubated DM). The gas volume of non-fibrous carbohydrate fermentation and fibrous carbohydrate degradation rate exhibited a quadratic effect according increasing levels of CC (Pmax = 265.8 g kg-1 DM and Pmin = 376.3 g kg-1 DM, respectively). The lag time and final gas volume showed a decreasing linear effect with increasing levels of CC protein. The degradation rate of non-fibrous carbohydrates and the final volume of fibrous carbohydrates did not differ. Replacing soybean meal protein with CC protein at the level of 250 g kg-1 of dry matter in diets formulated for feedlot lambs leads to good profiles of ruminal fermentation kinetics with respect to the degradation of fibrous and non-fibrous carbohydrates.

scholarly journals Manipulation of In Vitro Ruminal Fermentation and Feed Digestibility as Influenced by Yeast Waste-Treated Cassava Pulp Substitute Soybean Meal and Different Roughage to Concentrate Ratio

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 196
Author(s):  
Gamonmas Dagaew ◽  
Anusorn Cherdthong ◽  
Sawitree Wongtangtintharn ◽  
Metha Wanapat ◽  
Chanon Suntara
Keyword(s):  
Soybean Meal ◽  
Bacterial Population ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Partial Replacement ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Cassava Pulp ◽  
Feed Digestibility

Cassava pulp (CS) is high in fiber and low in protein; hence, improving the nutritive value of CS is required to increase its contribution to enhancing ruminant production. The present work hypothesized that CS quality could be enhanced by fermentation with yeast waste (YW), which can be used to replace soybean meal (SBM), as well as lead to improved feed utilization in ruminants. Thus, evaluation of in vitro ruminal fermentation and feed digestibility, as influenced by YW-treated CS and different roughage (R) to concentrate (C) ratios, was elucidated. The design of the experiment was a 5 × 3 factorial arrangement in a completely randomized design. Each treatment contained three replications and three runs. The first factor was replacing SBM with CS fermented with YW (CSYW) in a concentrate ratio at 100:0, 75:25, 50:50, 25:75, and 0:100, respectively. The second factor was R:C ratios at 70:30, 50:50, and 30:70. The level of CSYW showed significantly higher (p < 0.01) gas production from the insoluble fraction (b), potential extent of gas production (a + b), and cumulative gas production at 96 h than the control group (p < 0.05). There were no interactions among the CSYW and R:C ratio on the in vitro digestibility (p > 0.05). Furthermore, increasing the amount of CSYW to replace SBM up to 75% had no negative effect on in vitro neutral detergent fiber degradability (IVNDFD) (p > 0.05) while replacing CSWY at 100% could reduce IVNDFD (p > 0.05). The bacterial population in the rumen was reduced by 25.05% when CSYW completely replaced SBM (p < 0.05); however, 75% of CSWY in the diet did not change the bacterial population (p > 0.05). The concentration of propionate (C3) decreased upon an increase in the CSYW level, which was lowest with the replacement of SBM by CSYW up to 75%. However, various R:C ratios did not influence total volatile fatty acids (VFAs), and the proportion of VFAs (p > 0.05), except the concentration of C3, increased when the proportion of a concentrate diet increased (p < 0.05). In conclusion, CSYW could be utilized as a partial replacement for SBM in concentrate diets up to 75% without affecting gas kinetics, ruminal parameters, or in vitro digestibility.

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