Impact of non-protein nitrogen supplements on nematode infected sheep

2003 ◽  
Vol 43 (12) ◽  
pp. 1463 ◽  
Author(s):  
M. R. Knox

Inadequate nutrition and gastrointestinal nematode parasitism are major constraints to livestock production. In livestock rearing enterprises that rely on low quality roughage for the provision of digestible carbohydrate, the most critical nutritional deficiency is often nitrogen. The provision of non-protein nitrogen in the diet can compensate for this deficiency and enable increased productivity from the available feed resource. Numerous studies have shown that increasing the supply of protein for intestinal absorption can alleviate the detrimental effects of nematode parasites on production and can also result in improved protective immunological responses to infection. In many situations, the practical implementation of such a strategy for improved nematode parasite control is hampered by the high cost and/or unavailability of high quality protein sources for use as ruminant livestock feed. Evidence is presented from studies with young sheep to demonstrate that supplementation with urea can achieve similar qualitative benefits in reducing the effects and level of infection with parasitic nematodes to that achieved with protein supplements. Urea–molasses blocks are a popular low-cost means of delivering non-protein nitrogen and experimental evidence shows that the use of urea–molasses blocks can have a beneficial impact on enhancing the resilience and resistance of sheep to infection with nematode parasites. Consistent with other nutritional studies, urea appeared to confer increased benefits proportional to increasing supplement intake as shown by reduced pathological effects, parasite numbers and faecal egg output. It is suggested that application of these findings in Australian pasture systems may require revision of current systems for the delivery of non-protein nitrogen supplements to increase the level of urea consumed.

2020 ◽  
Vol 3 (2) ◽  
pp. 7-11
Author(s):  
A. Saidi ◽  
R. Mimouni ◽  
F. Hamadi ◽  
W. Oubrou

Monitoring of gastrointestinal nematode parasites in ruminants (domestic and wild) is often based on fecal examination techniques, looking for excreted eggs and larval forms using morphological keys. These, are more available in domestic ruminants, in which helminths are widely studied, than in wild ruminants.  This study tried to provide certain morphological elements that will help to recognize the L3 larvae of Camelostrongylus mentulatus and Nematodirus spathiger that could parasite either domestic or wild ruminants. For that, we resorted first to the culture of L3 larvae from fecal samples taken from African antelopes, and second by the microscopic characterization of each isolated larval morphological pattern previously identified by sequencing of its internal transcribed spacer (ITS-2) regions of the ribosomal DNA. The results of different microscopic captured images showed that Camelostrongylus mentulatus larva is 16 intestinal cells that measuring approximately 820 µm length, ‎≈ 25 µm wide, and ‎≈ 47 µm for its sheath tail extension and by this be closer to Teladorsagia circumcincta characteristics.  For Nematodirus spathiger, it possesses 8 gut cells and measuring about 1020 µm long, ‎≈ 25 µm wide, and‎ ≈ 143 µm for its sheath tail extension with specific tail appendages. Have done this, we were able to get some clarifications on the morphology of the studied larvae, and we believe thus that this study will contribute to the establishment of morphological identification keys especially for parasitic nematodes of wild ruminants.


2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
C. Y. Lee ◽  
K. A. Munyard ◽  
K. Gregg ◽  
J. D. Wetherall ◽  
M. J. Stear ◽  
...  

Gastrointestinal nematode parasites in farmed animals are of particular importance due to their effects on production. In Australia, it is estimated that the direct and indirect effects of parasite infestation cost the animal production industries hundreds of millions of dollars each year. The main factors considered by immunologists when studying gastrointestinal nematode infections are the effects the host's response has on the parasite, which immunological components are responsible for these effects, genetic factors involved in controlling immunological responses, and the interactions between these forming an interconnecting multilevel relationship. In this paper, we describe the roles of immunoglobulins, in particular IgA and IgE, and the major histocompatibility complex in resistance to gastrointestinal parasites in sheep. We also draw evidence from other animal models to support the involvement of these immune components. Finally, we examine how IgA and IgE exert their influence and how methods may be developed to manage susceptible animals.


2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Amy H. Buck ◽  
Gillian Coakley ◽  
Fabio Simbari ◽  
Henry J. McSorley ◽  
Juan F. Quintana ◽  
...  

Abstract In mammalian systems RNA can move between cells via vesicles. Here we demonstrate that the gastrointestinal nematode Heligmosomoides polygyrus, which infects mice, secretes vesicles containing microRNAs (miRNAs) and Y RNAs as well as a nematode Argonaute protein. These vesicles are of intestinal origin and are enriched for homologues of mammalian exosome proteins. Administration of the nematode exosomes to mice suppresses Type 2 innate responses and eosinophilia induced by the allergen Alternaria. Microarray analysis of mouse cells incubated with nematode exosomes in vitro identifies Il33r and Dusp1 as suppressed genes, and Dusp1 can be repressed by nematode miRNAs based on a reporter assay. We further identify miRNAs from the filarial nematode Litomosoides sigmodontis in the serum of infected mice, suggesting that miRNA secretion into host tissues is conserved among parasitic nematodes. These results reveal exosomes as another mechanism by which helminths manipulate their hosts and provide a mechanistic framework for RNA transfer between animal species.


Parasitology ◽  
2007 ◽  
Vol 134 (8) ◽  
pp. 1087-1092 ◽  
Author(s):  
ROGER K. PRICHARD

SUMMARYBenzimidazole (BZ) resistance is widespread and appears to be readily selected in a variety of nematode parasites of animals. There have been reports of a lack of efficacy of BZ anthelmintics against soil transmitted nematode parasites of humans. However, resistance to BZs in nematodes of humans has not been confirmed. It is difficult to perform tests to confirm anthelmintic resistance in humans for a variety of technical and ethical reasons. The use of anthelmintic drugs for the control of helminth parasites in people is increasing massively as a result of numerous programmes to control gastrointestinal nematode parasites in children, the Global Program for the Elimination of Lymphatic Filariasis and other programmes. Many of these programmes are dependent on BZ anthelmintics and this will increase the pressure for resistance development to BZ anthelmintics in nematode parasites of people. We need to perform monitoring for anthelmintic resistance in these programmes and we need new tools to make that monitoring sensitive, inexpensive and practical. There is a real need for DNA-based markers for BZ resistance in nematode parasites of humans. We have a reasonable understanding of the molecular mechanisms and genetics of BZ resistance in some nematode parasites of animals and similar mechanisms are likely to prevail in nematodes of humans. Based on the likelihood that similar single nucleotide polymorphisms (SNPs) will be involved in BZ resistance in human, as in animal nematode parasites, rapid SNP assays have been developed for possible BZ resistance development in Wuchereria bancrofti.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yehe Liu ◽  
Andrew M. Rollins ◽  
Richard M. Levenson ◽  
Farzad Fereidouni ◽  
Michael W. Jenkins

AbstractSmartphone microscopes can be useful tools for a broad range of imaging applications. This manuscript demonstrates the first practical implementation of Microscopy with Ultraviolet Surface Excitation (MUSE) in a compact smartphone microscope called Pocket MUSE, resulting in a remarkably effective design. Fabricated with parts from consumer electronics that are readily available at low cost, the small optical module attaches directly over the rear lens in a smartphone. It enables high-quality multichannel fluorescence microscopy with submicron resolution over a 10× equivalent field of view. In addition to the novel optical configuration, Pocket MUSE is compatible with a series of simple, portable, and user-friendly sample preparation strategies that can be directly implemented for various microscopy applications for point-of-care diagnostics, at-home health monitoring, plant biology, STEM education, environmental studies, etc.


2003 ◽  
Vol 47 (3) ◽  
pp. 193-202 ◽  
Author(s):  
E Papadopoulos ◽  
G Arsenos ◽  
S Sotiraki ◽  
C Deligiannis ◽  
T Lainas ◽  
...  

2001 ◽  
Vol 79 (7) ◽  
pp. 1706 ◽  
Author(s):  
N Mandonnet ◽  
G Aumont ◽  
J Fleury ◽  
R Arquet ◽  
H Varo ◽  
...  

2020 ◽  
Vol 31 (3) ◽  
pp. 145-159
Author(s):  
Haladu Ali Gagman ◽  
Nik Ahmad Irwan Izzauddin Nik Him ◽  
Hamdan Ahmad ◽  
Shaida Fariza Sulaiman ◽  
Rahmad Zakaria ◽  
...  

Gastrointestinal nematode infections can cause great losses in revenue due to decrease livestock production and animal death. The use of anthelmintic to control gastrointestinal nematode put a selection pressure on nematode populations which led to emergence of anthelmintic resistance. Because of that, this study was carried out to investigate the efficacy of aqueous and methanol extract of Cassia siamea against the motility of C. elegans Bristol N2 and C. elegans DA1316. Caenorhabditis elegans Bristol N2 is a susceptible strain and C. elegans DA1316 is an ivermectin resistant strain. In vitro bioassay of various concentrations of (0.2, 0.6, 0.8, 1.0 and 2.0 mg mL–1) aqueous and methanol extracts of C. siamea was conducted against the motility of L4 larvae of C. elegans Bristol N2 and C. elegans DA1316. The L4 larvae were treated with 0.02 μg mL–1 of ivermectin served as positive control while those in M9 solution served as negative control. The activity of the extracts was observed after 24 h and 48 h. A significant difference was recorded in the extract performance compared to control at (P < 0.001) after 48 h against the motility of the larvae of both strains. The methanol extracts inhibited the motility of C. elegans Bristol N2 by 86.7% as well as DA1316 up to 84.9% at 2.0 mg mL–1 after 48 h. The methanol extract was more efficient than aqueous extract (P < 0.05) against the motility of both strains of C. elegans. Cassia siamea may be used as a natural source of lead compounds for the development of alternative anthelmintic against parasitic nematodes as well ivermectin resistant strains of nematodes.


Author(s):  
S. R. PATIL ◽  
SNEHAL SALUNKHE ◽  
NIKITA KULKARNI ◽  
PRIYANKA SAVANT

ample efforts have been taken in restaurant sector to intrigue the dining experience. Several information and communication technologies have been adopted earlier such as PDA; wireless communication technologies etc.These technologies provide a simple but powerful infrastructure. This paper highlights the limitations of the existing technologies and proposed the E-CONVERSE, which focuses on low cost touch-screen development to enhance the dining experience. In this paper we discuss the design and implementation of a low cost, customizable touch screen. To ensure the security of the system some security strategies are discussed. Basic level testing reveals that proposed system has potential for practical implementation and can overcome several drawbacks of existing system.


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