110 COMPARISON OF ANDROMED®, BIOXCELL®, AND BOTU-BOV® EXTENDERS FOR CRYOPRESERVATION OF BULL SEXED SEMEN

2007 ◽  
Vol 19 (1) ◽  
pp. 172 ◽  
Author(s):  
M. Q. Braga ◽  
R. V. R. Franco ◽  
L. F. Rodrigues ◽  
G. Galeli ◽  
K. M. Oliveira ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Semen Quality ◽  
Membrane Damage ◽  
Optical Microscope ◽  
Hoechst 33342 ◽  
Tukey Test ◽  
Progressive Motility ◽  
Significant Difference ◽  
Sexed Semen ◽  
Fort Collins

Sexing semen has become a worldwide technology now available in many countries through the use of flow cytometry for sexing mammal sperms (Johnson and Welch 1999 Theriogenology 52, 1323–1341). Because straws containing sexed semen have a low concentration, any condition that either improves or decreases freezing capabilities will considerably change semen quality. During cryopreservation, spermatozoa have been described as undergoing many changes that lead to membrane damage, which may result in decreased fertility (Watson 2000 Reprod. Fertil. Dev. 6 (Suppl 1), 481). Since many cryoprotectants are available on the market, the objective of the present study was to compare 3 different extenders for freezing sex-sorted semen. For this study, 25 ejaculates were collected from 8 bulls of different breeds, diluted, then dyed with Hoechst 33342 (Schenk et al. 1999 Theriogenology 52, 1375–1391), and sexed by flow cytometry (SX MoFlo®; DaKoCytomation, Inc., Fort Collins, CO, USA). After being cooled at 4°C for 1 h and 30 min, the sexed semen was centrifuged and diluted in AndroMed® (Minitüb, Tiefenbach, Germany), Bioxcell® (IMV, Aigle, France), or Botu-Bov® (Biotech Botucatu, Ltda., Sao Paulo, Brazil); the semen was packaged at 3 million total sperm in 0.25-mL straws and frozen in an automatic freezer (Digit cool 5300® IMV). To evaluate the freezing quality, the straws were thawed and incubated at 35°C for 15 min. The progressive motility was observed through an optical microscope (Coleman 200T). The statistical analyses were done using the SAS program (SAS Institute, Inc., Cary, NC, USA) and the Tukey test (P ≤ 0.05). Results show that there was no statistical difference between Bioxcell and AndroMed extenders (P ≤ 0.05). However, Botu-Bov extender showed a significant difference when compared with Bioxcell and AndroMed (see Table 1). It is also important to point out that 40% of the samples frozen with AndroMed showed non-aligned movement. Even though few ejaculates were used for this study, preliminary results showed that Bioxcell seemed to be the most suitable for freezing bull sexed semen. Table 1. Percentage of progressively motile spermatozoa after thawing

scholarly journals Comparison of semen characteristics between indigenous and Amo breeds cockerel of Gombe State, Nigeria

2019 ◽  
Vol 15 (2-1) ◽  
pp. 303-306
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Ma’aruf Bashir Sani
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Indigenous Breed ◽  
Normal Sperm ◽  
Abnormal Sperm ◽  
Significant Difference ◽  
Semen Characteristics ◽  
Genetically Improved ◽  
Abdominal Massage

The experiment was carried out to compare the semen characteristics of indigenous and Amo strains of cockerel at poultry unit of teaching and research farm of Federal University of Kashere, Gombe State, Nigeria. Semen samples were collected from nine indigenous and nine Amo breeds of cockerel at three days interval for two weeks using abdominal massage technique. Semen samples were examined macroscopically for semen colour, pH and ejaculation volume. Then, microscopic observation was carried for sperm concentration, mass motility, progressive motility, live and dead sperms percentage, normal and abnormal sperm, all for semen characteristics. The results showed a significant difference (P ≤ 0.05) between mass motility, progressive motility, sperm concentration and head defects of 4.85 ± 0.27 to 4.37 ± 0.19, 95.13 ± 0.43 to 81.63 ± 1.15%, 4.93 ± 1.84 to 3.40 ± 1.07×109/ml and 2.96 ± 0.17 to 3.44 ± 0.12% for indigenous and Amo breeds of cockerel, respectively. There were no significant differences observed as semen colour, ejaculate volume, semen pH, live / dead normal sperm neck (mid-piece), tail defects and sperm total abnormalities were found to be 2.85 ± 0.07 to 2.00 ± 0.090.21 ± 0.17 to 0.20 ± 0.02 /ml, 88.85 ± 0.58 to 72.70 ± 0.54% /ml, 11.14 ± 0.58 to 27.29 ± 0.54%, 81.00 ± 0.78 to 66.22 ± 0.61%,9.03 ± 0.42 to 13.96 ± 0.47%, 9.70 ±  to 13.00 ± 0.30 and 21.70 ± 0.59 to 30.40 ± 0.53% for the indigenous and Amo breed groups of cockerel, respectively. It was concluded that semen quality characteristics could be differed between genetically improved (Amo strain) and indigenous breed of cockerels.

scholarly journals 305 FIRST REPORT OF THE USE OF SEXED SEMEN BY FLOW CYTOMETRY IN BOS INDICUS

2005 ◽  
Vol 17 (2) ◽  
pp. 303
Author(s):  
L. Cattaneo ◽  
A. Galassi ◽  
R. Franco ◽  
A. Duarte ◽  
K. Mello ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Speed ◽  
Bos Indicus ◽  
Field Trials ◽  
The Body ◽  
Chi Square ◽  
Female Fetus ◽  
Mato Grosso ◽  
Significant Difference ◽  
Sexed Semen

The only method of sexing sperm that works in any practical sense is measuring DNA content of individual sperm by flow cytometry, and sorting them based on that information (Schenk and Seidel 2002 J. Anim. Sci. 80 (Suppl. 1), 188). This is, also, the first reference of the use of sexed semen in Brazil. The objective of this experiment was to compare the fertility of sexed and non-sexed conventional semen in Bos indicus purebreeds. Ejaculates from eight Nelore bulls were collected by artificial vagina, diluted, and stained with Hoeschst 33342 (Schenk et al. 1999 Theriogenology 52, 1375–1391). A high-speed flow cytometer (SX-MOFLO®, DakoCytomation, Inc., Fort Collins, CO, USA) was used for sorting X sperm. Sexed semen was then packaged at 3 million total sperm in 0.25-mL straws and frozen. The average estimated purity obtained by resort analysis of one straw per batch was 91.5%. An aliquot of each ejaculate was frozen unsexed at a concentration of 50 × 106 sperm/mL in 0.5-mL straws (control). A total of 283 Nelore heifers were synchronized with two doses of PGF2α 12 days apart and inseminated in the body of the uterus 12 h after heat detection. Field trials were carried out in four different states in Brazil: Minas Gerais (MG), Mato Grosso (MT), Mato grosso do Sul (MS), Rondônia (RO). Pregnancy diagnosis and fetal sex determination were performed by ultrasound 30 and 60 days post-AI, respectively. Data were analyzed by chi-square and Monte Carlo methods. There was no significant difference (P < 0.05) in pregnancy rate between sexed and non-sexed semen (see Table 1). At the time of abstract submission, of 41 pregnant heifers from sexed semen confirmed by ultrasound, 40 had a female fetus (97%). These results indicate that sexing semen by flow cytometry could be a very important tool to accelerate the genetic gain in Bos indicus cattle. Brazil has the largest commercial herd in the world and, therefore, a great potential to incorporate this technology that is now available in the country. Table 1.

scholarly journals Variance in Stallion Semen Quality among Equestrian Sporting Disciplines and Competition Levels

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 485
Author(s):  
Megan Wilson ◽  
Jess Williams ◽  
V. Tamara Montrose ◽  
Jane Williams
Keyword(s):  
Semen Quality ◽  
Well Being ◽  
Physiological Changes ◽  
Controversial Subject ◽  
Progressive Motility ◽  
Daily Exercise ◽  
Semen Volume ◽  
Significant Difference ◽  
Breeding Programmes ◽  
The Difference

Most stallions within breeding programmes are expected to breed and compete concurrently. The exercising of stallions with regards to training regimes during the breeding season is a controversial subject. Daily exercise at low intensities is important for the mental and reproductive well-being of the stallion, however higher intensities of exercise, as seen in competing stallions, may have detrimental effects on seminal quality. To calculate if competition does affect semen quality, this study investigated the effect that equestrian discipline and timing of competition had on a range of stallion semen characteristics. This was a retrospective study that evaluated the seminal data of 1130 stallion semen collections from two UK based stud farms between 2009 and 2016. Competing stallion semen quality was significantly lower with regards to concentration (p < 0.05) and progressive motility (p < 0.05) than non-competing stallions. Semen volume was higher in competing stallions (p < 0.05) than non-competing stallions. There was a significant difference in seminal attributes among disciplines and competition levels (p < 0.05). The difference in semen quality among competing and non-competing stallions, as well as the difference among disciplines suggests endocrinological and physiological changes occur in relation to training intensity and competition.

scholarly journals Effect of Fluoride-Treated Enamel on Indirect Cytotoxicity of a 16% Carbamide Peroxide Bleaching Gel to Pulp Cells

2013 ◽  
Vol 24 (2) ◽  
pp. 121-127 ◽  
Author(s):  
Diana Gabriela Soares ◽  
Ana Paula Dias Ribeiro ◽  
Adriano Fonseca Lima ◽  
Nancy Tomoko Sacono ◽  
Josimeri Hebling ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Membrane ◽  
Mtt Assay ◽  
Membrane Damage ◽  
Carbamide Peroxide ◽  
Cell Membrane Damage ◽  
Peroxide Bleaching ◽  
Alp Activity ◽  
Significant Difference ◽  
Pulp Cells

The aim of this study was to evaluate the possibility of fluoride solutions applied to enamel to protect pulp cells against the trans-enamel and transdentinal cytotoxicity of a 16% carbamide peroxide (CP) bleaching gel. The CP gel was applied to enamel/dentin discs adapted to aicial pulp chambers (8 h/day) during 1, 7 or 14 days, followed by fluoride (0.05% or 0.2%) application for 1 min. The extracts (culture medium in contact with dentin) were applied to MDPC-23 cells for 1 h, and cell metabolism (MTT assay), alkaline phosphatase (ALP) activity and cell membrane damage (flow cytometry) were analyzed. Knoop microhardness of enamel was also evaluated. Data were analyzed statistically by ANOVA and Kruskal-Wallis tests (α=0.05). For the MTT assay and ALP activity, significant reductions between the control and the bleached groups were observed (p<0.05). No statistically significant difference occurred among bleached groups (p>0.05), regardless of fluoride application or treatment days. Flow cytometry analysis demonstrated 30% of cell membrane damage in all bleached groups. After 14 days of treatment, the fluoride-treated enamel presented significantly higher microhardness values than the bleached-only group (p<0.05). It was concluded that, regardless of the increase in enamel hardness due to the application of fluoride solutions, the treated enamel surface did not prevent the toxic effects caused by the 16% CP gel to odontoblast-like cells.

scholarly journals Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2053
Author(s):  
Junsong Shi ◽  
Baohua Tan ◽  
Lvhua Luo ◽  
Zicong Li ◽  
Linjun Hong ◽  
...  
Keyword(s):  
Growth Rate ◽  
Body Size ◽  
Growth Performance ◽  
Reproductive Performance ◽  
Semen Quality ◽  
Small Body ◽  
Economic Benefits ◽  
Large Animal ◽  
Significant Difference ◽  
F1 Progeny

How to maximize the use of the genetic merits of the high-ranking boars (also called superior ones) is a considerable question in the pig breeding industry, considering the money and time spent on selection. Somatic cell nuclear transfer (SCNT) is one of the potential ways to answer the question, which can be applied to produce clones with genetic resources of superior boar for the production of commercial pigs. For practical application, it is essential to investigate whether the clones and their progeny keep behaving better than the “normal boars”, considering that in vitro culture and transfer manipulation would cause a series of harmful effects to the development of clones. In this study, 59,061 cloned embryos were transferred into 250 recipient sows to produce the clones of superior Pietrain boars. The growth performance of 12 clones and 36 non-clones and the semen quality of 19 clones and 28 non-clones were compared. The reproductive performance of 21 clones and 25 non-clones were also tested. Furthermore, we made a comparison in the growth performance between 466 progeny of the clones and 822 progeny of the non-clones. Our results showed that no significant difference in semen quality and reproductive performance was observed between the clones and the non-clones, although the clones grew slower and exhibited smaller body size than the non-clones. The F1 progeny of the clones showed a greater growth rate than the non-clones. Our results demonstrated through the large animal population showed that SCNT manipulation resulted in a low growth rate and small body size, but the clones could normally produce F1 progeny with excellent growth traits to bring more economic benefits. Therefore, SCNT could be effective in enlarging the merit genetics of the superior boars and increasing the economic benefits in pig reproduction and breeding.

scholarly journals POS0369 ELEVATED EXPRESSION OF TIM-3 ON NEUTROPHILS CORRELATES WITH DISEASE ACTIVITY AND SEVERITY OF ANKYLOSING SPONDYLITIS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 414.2-415
Author(s):  
X. Huang ◽  
T. W. Li ◽  
J. Chen ◽  
Z. Huang ◽  
S. Chen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Ankylosing Spondylitis ◽  
Disease Activity ◽  
Immune Cells ◽  
Clinical Manifestations ◽  
Innate Immune ◽  
Innate Immune Cells ◽  
Bacterial Killing ◽  
Markers Of Inflammation ◽  
Significant Difference

Background:Ankylosing spondylitis (AS) is a type of common, chronic inflammatory disease that compromises the axial skeleton and sacroiliac joints, causing inflammatory low back pain and progressive spinal stiffness, over time some patients develop spinal immobility and ankylosis which can lead to a decrease in quality of life. The last few decades, evidence has clearly indicated that neutrophil also plays key roles in the progression of AS. However, the immunomodulatory roles and mechanisms of neutrophils in AS are poorly understood. T-cell immunoglobulin and mucin domain-containing protein 3 (Tim-3) has been reported as an important regulatory molecule, expressed and regulated on different innate immune cells, plays a pivotal role in several autoimmunity diseases. Recent study indicates that Tim3 is also expressed on neutrophils. However, the frequency and roles of Tim3-expressing neutrophils in AS was not clear.Objectives:In this study, we investigated the expression of Tim3 on neutrophils in AS patients and explored the correlation between the level of Tim3-expressing neutrophils and the disease activity and severity of AS.Methods:Patients with AS were recruited from Guangdong Second Provincial General Hospital (n=62). Age/sex-matched volunteers as Healthy controls (HC) (n=39). The medical history, clinical manifestations, physical examination, laboratory measurements were recorded. The expression of costimulatory molecules including programmed death 1 (PD-1), Tim-3 on neutrophils were determined by flow cytometry. The mRNA expression of PD-1 and Tim-3 was determined by real-time PCR. The levels of Tim3-expressing neutrophils in AS patients were further analyzed for their correlation with the markers of inflammation such as ESR,CRP,WBC and neutrophil count(NE), as well as disease activity and severity of AS. The expression of Tim3 on neutrophils was monitored during the course of treatment (4 weeks).Results:The expression of Tim3 on neutrophils in patients with AS was increased compared to the HC (Figure 1A). However, significant difference was observed in the frequency of PD-1-expressing neutrophils between AS patients and HC (Figure 1B). The expression analysis of Tim-3 mRNA, but not PD-1, confirmed the results obtained from flow cytometry (Figure 1C). The level of Tim3-expressing neutrophils in patients with AS showed an positive correlation with ESR, CRP and ASAS-endorsed disease activity score (ASDAS) (Figure 1D). Moreover, the frequency of Tim3-expressing neutrophils in active patients(ASDAS≥1.3) was increased as compare with the inactive patients (ASDAS<1.3) (Figure 1E). As shown in Figure 1F, the frequency of Tim3-expressing neutrophils decreased after the treatment.Conclusion:Increased Tim-3 expression on neutrophils may be a novel indicator to assess disease activity and severity in AS, which may serves as a negative feedback mechanism preventing potential tissue damage caused by excessive inflammatory responses in AS patients.References:[1]Han, G., Chen, G., Shen, B. & Li, Y., Tim-3: an activation marker and activation limiter of innate immune cells. FRONT IMMUNOL 4 449 (2013).[2]Vega-Carrascal, I. et al., Galectin-9 signaling through TIM-3 is involved in neutrophil-mediated Gram-negative bacterial killing: an effect abrogated within the cystic fibrosis lung. J IMMUNOL 192 2418 (2014).Figure 1.(A,B)The expression of Tim3 and PD-1 on neutrophils in AS and HC were determined by flow cytometry.(C) The expression of Tim3 and PD-1 on neutrophils in AS and HC were determined by RT-PCR.(D)The correction between Tim3-expressing neutrophils and ESR,CRP,ASDAS.(E) The expression of Tim3 on neutrophils in active and inactive patients.(F) Influence of treatment on the frequency of Tim3-expressing neutrophils.Disclosure of Interests:None declared

scholarly journals Nanotubular Oxide Layer Formed on Helix Surfaces of Dental Screw Implants

Coatings ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 115 ◽  
Author(s):  
Magdalena Jażdżewska ◽  
Michał Bartmański
Keyword(s):  
Oxide Layer ◽  
Cross Sections ◽  
Surface Coverage ◽  
Artificial Saliva ◽  
Corrosion Current ◽  
Optical Microscope ◽  
Significant Difference ◽  
Helix Surfaces ◽  
Future Work ◽  
Oxidation Parameters

Surface modification is used to extend the life of implants. To increase the corrosion resistance and improve the biocompatibility of metal implant materials, oxidation of the Ti-13Nb-13Zr titanium alloy was used. The samples used for the research had the shape of a helix with a metric thread, with their geometry imitating a dental implant. The oxide layer was produced by a standard electrochemical method in an environment of 1M H3PO4 + 0.3% HF for 20 min, at a constant voltage of 30 V. The oxidized samples were analyzed with a scanning electron microscope. Nanotubular oxide layers with internal diameters of 30–80 nm were found. An analysis of the surface topography was performed using an optical microscope, and the Sa parameter was determined for the top of the helix and for the bottom, where a significant difference in value was observed. The presence of the modification layer, visible at the bottom of the helix, was confirmed by analyzing the sample cross-sections using computed tomography. Corrosion tests performed in the artificial saliva solution demonstrated higher corrosion current and less noble corrosion potential due to incomplete surface coverage and pitting. Necessary improved oxidation parameters will be applied in future work.

scholarly journals Total Organic Carbon Assessment in Soils Cultivated with Agave tequilana Weber in Jalisco, Mexico

2020 ◽  
Vol 13 (1) ◽  
pp. 208
Author(s):  
Celia De La Mora-Orozco ◽  
José G. Flores-Garnica ◽  
Lucia M. Vega-Ramírez ◽  
Irma J. González-Acuña ◽  
Juan Nápoles-Armenta ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Total Organic Carbon ◽  
Management Practices ◽  
Water Saturation ◽  
Management Strategies ◽  
Information Criterion ◽  
Agave Tequilana ◽  
Tukey Test ◽  
Significant Difference ◽  
Agriculture Management

The Agave tequilana Weber is an important commercial crop in the State of Jalisco, Mexico. However, the agave cultivation generates significant soil loss. For that reason, knowledge about the implementation of the agriculture management practices, such as manure application and the combination of inorganic fertilizers and manure, are relevant. The objective of this research was to determine the effect of agricultural management practices on the total organic carbon (TOC) in the soil in three study locations: Arandas, Tepatitlán, and Acatic in the Altos Sur region of Jalisco. A random sampling was carried out in each study location, 12 samples were obtained for each location at 0–30 cm deep, and a total of 36 samples were analyzed. The evaluated parameters were the potential hydrogen (pH), electrical conductivity (EC), bulk density (BD), soil-water saturation (SWS), total nitrogen (TN), and total organic carbon (TOC). Basic statistics and correlations between parameters were generated. In addition, to estimate TOC from a multivariate analysis, models were developed based on the lowest Akaike information criterion (AIC) and of the classification and regression trees (CART). ANOVA and Tukey test were determined. Results demonstrated a significant difference in the TOC percentages between the study locations. The Tukey test showed that there is no difference in TOC content between the Tepatitlán and Arandas sites, but there is a difference between these two sites and the Acatic. The latter resulted with the lowest values of TOC. Long-term studies are recommended to develop crop management strategies.

scholarly journals Viability of Najawa carp (Cyprinus carpio L.) sperm at 4°C temperature storage

2020 ◽  
Vol 147 ◽  
pp. 01015
Author(s):  
Dimas Fendy Pradana ◽  
Ignatius Hardaningsih ◽  
Dini Wahyu Kartika Sari
Keyword(s):  
Low Temperature ◽  
Cyprinus Carpio ◽  
Sperm Viability ◽  
Low Temperature Storage ◽  
Progressive Motility ◽  
C Storage ◽  
Randomized Design ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Temperature Storage

The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose.

Semen quality and interval to sterility in tom cats treated with a 9.4 mg deslorelin implant

2016 ◽  
Vol 19 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Stefano Romagnoli ◽  
Anna Baldan ◽  
Camilla Righetti ◽  
Chiara Milani ◽  
Antonio Mollo ◽  
...  
Keyword(s):  
Intramuscular Injection ◽  
Healthy Adult ◽  
Semen Quality ◽  
Serum Testosterone ◽  
Reproductive Behaviour ◽  
Gonadotropin Releasing Hormone ◽  
Gnrh Agonists ◽  
Progressive Motility ◽  
Semen Collection ◽  
Very High

Objectives Gonadotropin-releasing hormone (GnRH) agonists like deslorelin are being increasingly used in tom cats for their efficacy in controlling reproductive behaviour and fertility. Deslorelin implants have been widely available in Europe since 2008. Little, if anything, is known about the interval between treatment and onset of sterility, as well as semen quality, after treatment in tom cats. The purpose of this study was to investigate semen quality and interval to sterility in tom cats treated with a 9.4 mg deslorelin implant. Methods Fifteen healthy adult tom cats were treated with a 9.4 mg deslorelin implant (Suprelorin 12). For each cat, semen collection and a GnRH stimulation test (intramuscular administration of 50 μg gonadorelin [Fertagyl], followed by blood sampling 1 h later, to assay serum testosterone) were performed on the first consultation and then repeated every 15 days until complete sterility was achieved. Semen collection was performed by introducing a 14 cm, open-end feline catheter (Argyle) 9 cm into the distal urethra 10 mins after sedation by intramuscular injection of 100 μg/kg medetomidine (Domitor). Results Semen collection was not successful in all cats at each attempt. In the first month after treatment, the semen of only four cats could be evaluated, while the semen of eight cats could be evaluated during the second and third months of the study. Semen quality (ejaculate volume, progressive motility and morphological abnormalities) improved slightly during the first 19–25 days in 2/4 cats, and in 1/4 cats motility was still very high (80%) 25 days post-treatment (PT), but we have no data regarding fertility prior to treatment in this cat. The last cat never produced spermatozoa. Subsequently, semen quality gradually worsened in all cats from 30 days onwards. At 70 days PT, one cat was still potentially fertile. After 72 days all cats were sterile. Conclusions and relevance Semen quality increased slightly in treated cats during the first month after treatment, and then gradually decreased over the following months. Complete sterility was reached within 40–72 days following implantation.

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