Age estimation of pteropodid bats (Megachiroptera) from hard tissue parameters

1994 ◽  
Vol 21 (3) ◽  
pp. 353 ◽  
Author(s):  
SM Cool ◽  
MB Bennet ◽  
K Romaniuk

This investigation employed undecalcified thin (10 �m) and thick (100 �m) mid-root sections of surgically removed canine teeth, and thick sections of diaphyseal fore- and hind-limb bones from 14 fruit bats (Pteropus alecto and R poliocephalus) of known age, to attempt to establish a relationship between the chronological age of these animals and changes in the cross-sectional morphology of the hard tissues. Growth layers in bone, dentine and cementum were clearly visible in cross sections when viewed by Nomarski interference microscopy. The number of growth layers in the periosteal region of long bones and in dentine varied widely within a given section, making it impossible to estimate age from these tissues. Dental cementum most reliably reflected the age of fruit bats, with both growth layers and the radial thickness of cementum showing significant correlations with age (P<0.05). Linear regression was used to formulate equations for estimating age. The simplicity of this technique may provide investigators with a useful method for estimating the age of pteropodid fruit bats.

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
P Parsi-Pour ◽  
B M Kilbourne

Synopsis Locomotor habits in mammals are strongly tied to limb bones’ lengths, diameters, and proportions. By comparison, fewer studies have examined how limb bone cross-sectional traits relate to locomotor habit. Here, we tested whether climbing, digging, and swimming locomotor habits reflect biomechanically meaningful differences in three cross-sectional traits rendered dimensionless— cross-sectional area (CSA), second moments of area (SMA), and section modulus (MOD)—using femora, tibiae, and fibulae of 28 species of mustelid. CSA and SMA represent resistance to axial compression and bending, respectively, whereas MOD represents structural strength. Given the need to counteract buoyancy in aquatic environments and soil’s high density, we predicted that natatorial and fossorial mustelids have higher values of cross-sectional traits. For all three traits, we found that natatorial mustelids have the highest values, followed by fossorial mustelids, with both of these groups significantly differing from scansorial mustelids. However, phylogenetic relatedness strongly influences diversity in cross-sectional morphology, as locomotor habit strongly correlates with phylogeny. Testing whether hind limb bone cross-sectional traits have evolved adaptively, we fit Ornstein–Uhlenbeck (OU) and Brownian motion (BM) models of trait diversification to cross-sectional traits. The cross-sectional traits of the femur, tibia, and fibula appear to have, respectively, diversified under a multi-rate BM model, a single rate BM model, and a multi-optima OU model. In light of recent studies on mustelid body size and elongation, our findings suggest that the mustelid body plan—and perhaps that of other mammals—is likely the sum of a suite of traits evolving under different models of trait diversification.


1982 ◽  
Vol 98 (3) ◽  
pp. 629-632
Author(s):  
H. J. Swatland

SUMMARYHindlimbs of pigs were dissected at 4-week intervals from 9 to 29 weeks after birth. Allometric growth ratios of the gluteal muscles, biceps femoris and semitendinosus were calculated relative to gastrocnemius. Relative growth in weight of the proximal limb muscles was greater than that of the distal muscle, gastrocnemius. The greater relative growth of proximal muscles was not due to greater relative growth of muscle fibre diameters, nor to greater relative growth of muscle cross-sectional areas. In biceps femoris and gluteal muscles, growth in the area of musole cross sections did not keep pace with the radial growth of muscle fibres. The relative longitudinal growth of proximal limb bones exceeded that of distal bones, and the relative longitudinal growth of biceps femoris and semitendinosus exceeded that of any of the limb bones. New sarcomeres were added to muscle length at a faster rate in biceps femoris and semitendinosus than in the peroneus longus located distally in the limb.


Author(s):  
Elizabeth Weiss

Cross-sectional geometries are examined in chapter 2; long bones can be envisioned as beams that experience biomechanical stresses, such as bending, torsion, and compressive forces, to which they remodel to prevent from breaking. Using cross-sectional measures, such as moments of inertia that represent bone’s ability to resist bending and torsional stresses, researchers can determine the direction of stresses and, therefore, the activities that cause these stresses. One common activity examined is mobility, which leads to anteroposteriorly oriented femoral cross-sections due to bending stresses. Asymmetry studies on tennis players have helped confirm that cross-sectional geometries change in response to activities. Yet animal studies, such as those on baboons, have been key in helping bioarchaeologists understand the non-activity pressures, such as age, sex, and genes, on cross-sectional geometries.


Author(s):  
T.B. Ball ◽  
W.M. Hess

It has been demonstrated that cross sections of bundles of hair can be effectively studied using image analysis. These studies can help to elucidate morphological differences of hair from one region of the body to another. The purpose of the present investigation was to use image analysis to determine whether morphological differences could be demonstrated between male and female human Caucasian terminal scalp hair.Hair samples were taken from the back of the head from 18 caucasoid males and 13 caucasoid females (Figs. 1-2). Bundles of 50 hairs were processed for cross-sectional examination and then analyzed using Prism Image Analysis software on a Macintosh llci computer. Twenty morphological parameters of size and shape were evaluated for each hair cross-section. The size parameters evaluated were area, convex area, perimeter, convex perimeter, length, breadth, fiber length, width, equivalent diameter, and inscribed radius. The shape parameters considered were formfactor, roundness, convexity, solidity, compactness, aspect ratio, elongation, curl, and fractal dimension.


JKCD ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 9-11
Author(s):  
Sadaf Ambreen

Objectives: To compare Demirjian Dental scoring method with Greulich-Pyle (GP) Skeletal method of age estimation in pubertal children. Materials and Methods: Sample of the study included 267 male healthy subjects of 11-16 years of age group.. Demirjian Scoring system was utilized to evaluate the orthopantomograms to assess their Dental age and the Hand-Wrist radiographs were analyzed to calculate the skeletal age by utilizing GP atlas. Chronological age was obtained from the date of birth of the subject .Both methods were compared with one another and with the chronological age. It was a cross-sectional study and only healthy male subjects without any clinical abnormalities were included in the study. Results: A total of 267 male subjects of 11-16 years of age group were assessed by Demirjian and Greulich Pyle Methods. Both were compared with Chronological Age. Data obtained was statistically analyzed and the Student “t” test was applied in the study population. The mean difference between Chronolgical age and dental age was 0.69years and that of chronological age and skeletal age was 0.87 years. It was observed from dental age assessment that it does not differ much from the skeletal age. Conclusion: It was concluded that Demirjian method of Age Estimation is more precise than Greulich Pyle method of Age Estimation. Furthermore both methods can be used selectively in Medicolegal cases to access bone age which can be easily correlated to chronological age.


2012 ◽  
Vol 27 (2) ◽  
pp. 264-269 ◽  
Author(s):  
Christian Lorbach ◽  
Ulrich Hirn ◽  
Johannes Kritzinger ◽  
Wolfgang Bauer

Abstract We present a method for 3D measurement of fiber cross sectional morphology from handsheets. An automated procedure is used to acquire 3D datasets of fiber cross sectional images using an automated microtome and light microscopy. The fiber cross section geometry is extracted using digital image analysis. Simple sample preparation and highly automated image acquisition and image analysis are providing an efficient tool to analyze large samples. It is demonstrated that if fibers are tilted towards the image plane the images of fiber cross sections are always larger than the true fiber cross section geometry. In our analysis the tilting angles of the fibers to the image plane are measured. The resulting fiber cross sectional images are distorted to compensate the error due to fiber tilt, restoring the true fiber cross sectional shape. We use an approximated correction, the paper provides error estimates of the approximation. Measurement results for fiber wall thickness, fiber coarseness and fiber collapse are presented for one hardwood and one softwood pulp.


2017 ◽  
Vol 34 (1) ◽  
pp. 8-17 ◽  
Author(s):  
Stephanie Goldschmidt ◽  
Catherine Zimmerman ◽  
Caitlyn Collins ◽  
Scott Hetzel ◽  
Heidi-Lynn Ploeg ◽  
...  

Biomechanical studies of the elongated canine tooth of animals are few, and thus our understanding of mechanical and physical properties of animal teeth is limited. The objective of the present study was to evaluate the influence of force direction on fracture resistance and fracture pattern of canine teeth in an ex vivo dog cadaver model. Forty-five extracted canine teeth from laboratory beagle dogs were standardized by hard tissue volume and randomly distributed among 3 force direction groups. The teeth were secured within a universal testing machine and a load was applied at different directions based on testing group. The maximum force to fracture and the fracture pattern classification were recorded for each tooth. After correcting for hard tissue cross-sectional area in a multivariate analysis, no significant difference in the amount of force required for fracture was apparent between the different force direction groups. However, the influence of force direction on fracture pattern was significant. The results of this study may allow the clinician to educate clients on possible causal force directions in clinically fractured teeth and, thus, help prevent any contributing behavior in the future.


2006 ◽  
Vol 7 (3) ◽  
pp. 163-174 ◽  
Author(s):  
Myoung-Ae Choe ◽  
Gyeong Ju An ◽  
Yoon-Kyong Lee ◽  
Ji Hye Im ◽  
Smi Choi-Kwon ◽  
...  

This study examined the effects of daily low-intensity exercise following acute stroke on mass, Type I and II fiber cross-sectional area, and myofibrillar protein content of hind-limb muscles in a rat model. Adult male Sprague-Dawley rats were randomly assigned to 1 of 4 groups (n = 7-9 per group): stroke (occlusion of the right middle cerebral artery [RMCA]), control (sham RMCA procedure), exercise, and stroke-exercise. Beginning 48 hours post-stroke induction/sham operation, rats in the exercise group had 6 sessions of exercise in which they ran on a treadmill at grade 10 for 20 min/day at 10 m/min. At 8 days poststroke, all rats were anesthetized and soleus, plantaris, and gastrocnemius muscles were dissected from both the affected and unaffected sides. After 6 sessions of exercise following acute ischemic stroke, the stroke-exercise group showed the following significant (p < .05) increases compared to the stroke-only group: body weight and dietary intake, muscle weight of affected soleus and both affected and unaffected gastrocnemius muscle, Type I fiber cross-sectional area of affected soleus and both affected and unaffected gastrocnemius muscle, Type II fiber cross-sectional area of the unaffected soleus, both affected and unaffected plantaris and gastrocnemius muscle, Type II fiber distribution of affected gastrocnemius muscle, and myofibrillar protein content of both affected and unaffected soleus muscle. Daily low-intensity exercise following acute stroke attenuates hind-limb muscle atrophy in both affected and unaffected sides. The effects of exercise are more pronounced in the soleus and gastrocnemius as compared to the plantaris muscle.


2021 ◽  
Vol 13 (6) ◽  
pp. 3255
Author(s):  
Aizhao Zhou ◽  
Xianwen Huang ◽  
Wei Wang ◽  
Pengming Jiang ◽  
Xinwei Li

For reducing the initial GSHP investment, the heat transfer efficiency of the borehole heat exchange (BHE) system can be enhanced to reduce the number or depth of drilling. This paper proposes a novel and simple BHE design by changing the cross-sectional shape of the U-tube to increase the heat transfer efficiency of BHEs. Specifically, in this study, we (1) verified the reliability of the three-dimensional numerical model based on the thermal response test (TRT) and (2) compared the inlet and outlet temperatures of the different U-tubes at 48 h under the premise of constant leg distance and fluid area. Referent to the circular tube, the increases in the heat exchange efficiencies of the curved oval tube, flat oval tube, semicircle tube, and sector tube were 13.0%, 19.1%, 9.4%, and 14.8%, respectively. (3) The heat flux heterogeneity of the tubes on the inlet and outlet sides of the BHE, in decreasing order, is flat oval, semicircle, curved oval, sector, and circle shapes. (4) The temperature heterogeneity of the borehole wall in the BHE in decreasing order is circle, sector, curved oval, flat oval, and semicircle shapes. (5) Under the premise of maximum leg distance, referent to the heat resistance of the tube with a circle shape at 48 h, the heat exchange efficiency of the curved oval, flat oval, semicircle, and sector tubes increased 12.6%, 17.7%, 10.3%, and 7.8%, respectively. (6) We found that the adjustments of the leg distance and the tube shape affect the heat resistance by about 25% and 12%, respectively. (7) The flat-oval-shaped tube at the maximum leg distance was found to be the best tube design for BHEs.


2010 ◽  
Vol 638-642 ◽  
pp. 675-680 ◽  
Author(s):  
Martina Thomann ◽  
Nina von der Höh ◽  
Dirk Bormann ◽  
Dina Rittershaus ◽  
C. Krause ◽  
...  

Current research focuses on magnesium based alloys in the course of searching a resorbable osteosynthetic material which provides sufficient mechanical properties besides a good biocompatibility. Previous studies reported on a favorable biocompatibility of the alloys LAE442 and MgCa0.8. The present study compared the degradation process of cylindrical LAE442 and MgCa0.8 implants after 12 months implantation duration. Therefore, 10 extruded implants (2.5 x 25 mm, cross sectional area 4.9 mm²) of both alloys were implanted into the medullary cavity of both tibiae of rabbits for 12 months. After euthanization, the right bone-implant-compound was scanned in a µ-computed tomograph (µCT80, ScancoMedical) and nine uniformly distributed cross-sections of each implant were used to determine the residual implants´ cross sectional area (Software AxioVisionRelease 4.5, Zeiss). Left implants were taken out of the bone carefully. After weighing, a three-point bending test was carried out. LAE442 implants degraded obviously slower and more homogeneously than MgCa0.8. The mean residual cross sectional area of LAE442 implants was 4.7 ± 0.07 mm². MgCa0.8 showed an area of only 2.18 ± 1.03 mm². In contrast, the loss in volume of LAE442 pins was more obvious. They lost 64 % of their initial weight. The volume of MgCa0.8 reduced clearly to 54.4 % which corresponds to the cross sectional area results. Three point bending tests revealed that LAE442 showed a loss in strength of 71.2 % while MgCa0.8 lost 85.6 % of its initial strength. All results indicated that LAE442 implants degraded slowly, probably due to the formation of a very obvious degradation layer. Degradation of MgCa0.8 implants was far advanced.


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