Effect of forage: concentrate ratio on ruminal metabolism and duodenal flow of fatty acids in beef steers

2006 ◽  
Vol 82 (1) ◽  
pp. 31-40 ◽  
Author(s):  
M. R. F. Lee ◽  
J. K. S. Tweed ◽  
R. J. Dewhurst ◽  
N. D. Scollan
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Linseed Oil ◽  
Nitrogen Concentration ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Significant Shift ◽  
Beef Steers ◽  
Microbial Nitrogen ◽  
Cla Isomers

AbstractThe objective of this study was to determine the proportion of forage in the diet which would maximize duodenal flow of unsaturated fatty acids in beef steers supplemented with linseed oil. A second objective was to determine how diets differing in forage content, but equal in nitrogen (N), energy and lipid supply, would affect the duodenal flow of C18:1 and conjugated linoleic acid (CLA) isomers. Eight Hereford×Friesian steers (533±13·6 kg), prepared with rumen and duodenal cannulae were offered one of four forage:concentrate (F:C) ratios: F80C20; F60C40; F40C60 and F20C80 on a dry-matter (DM) basis. All diets were offered at 0·013 body weight and designed to be isonitrogenous and isoenergetic with total lipid made up to 0·06 DM intake with linseed oil. The experimental design was a replicated incomplete 4×4 Latin square with three periods. Increasing the concentrate component in the diet from 0·20 to 0·60 reduced rumen pH from 6·58 to 6·37 and caused a small but significant shift in volatile fatty acid molar proportions, decreasing the non-glucogenic ratio. Rumen ammonia-nitrogen concentration was also significantly reduced with increasing concentrate, from 156·8 to 101·0 mg N per l on F80C20 and F20C80, respectively. Microbial nitrogen (MN) and the efficiency of microbial protein synthesis was significantly elevated as forage level decreased from 51·6 to 72·4 g/day and 17·2 to 27·3 g MN per kg organic matter apparently digested in the rumen, respectively for F80C20 and F20C80. Intake and duodenal flow of C18:1n-9 and C18:2n-6 were significantly higher with increasing concentrate level in the diet whereas C18:3n-3 intake and flow was not different, averaging 143·6 and 6·37 g/day, respectively. There were no differences in the flows of total C18:1 trans or CLA (47·7 and 1·79 g/day, respectively) across the diets. However, although not significantly different in duodenal flow there were trends (P<0·1) for an increasing proportion of trans-10 and a decreasing proportion of trans-11 when increasing the concentrate in the diet. Biohydrogenation of C18:2n-6 decreased from 0·91 to 0·85 when increasing concentrate in the diet from 0·20 to 0·40 but further increases had no effect. F:C ratio had little effect on the flow of unsaturated fatty acids, C18:1 trans and CLA to the duodenum of beef steers, and this may relate to the ability of the rumen to buffer the large changes in concentrate intake.

scholarly journals Dietary linseed oil and selenate affect the concentration of fatty acids in selected tissues of sheep

2012 ◽  
Vol 57 (No. 9) ◽  
pp. 389-401 ◽  
Author(s):  
M. Czauderna ◽  
J. Kowalczyk ◽  
M. Marounek
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Linseed Oil ◽  
Subcutaneous Fat ◽  
Biceps Femoris ◽  
Mass Gain ◽  
Control Group ◽  
Feed Conversion ◽  
Adipose Tissues ◽  
Cla Isomers

The aim of the study was to determine the influence of the addition of 5% linseed oil (LO) and 2 &micro;g Se as selenate (SeVI) per g of diet on body mass gain (BMG), feed conversion efficiency (FCE), and concentrations of fatty acids (FA), especially conjugated linoleic acid (CLA) isomers, in the liver, heart, m.&nbsp;longissimus dorsi (MLD), m. biceps femoris (MBF), subcutaneous fat (SF), perirenal fat (PF), and intermuscular fat (IF) of sheep. Each group comprised 5 lambs with an average initial body weight of 25 &plusmn; 2 kg. For 35 days the sheep were fed the control or experimental diets enriched with 5% LO, SeVI, or LO and SeVI combined. The diets with LO, regardless of the presence of SeVI, increased BMG, FCE, and the mass of MBF, MLD, and the liver. The LO treatment increased the concentration of total CLA isomers in the liver, SF, PF, and MLD in comparison with the control. Dietary LO with or without SeVI resulted in negligible changes in the concentration of total CLA isomers in MBF. The diet enriched with LO, irrespective of the presence of SeVI, increased the concentrations of cis- monounsaturated fatty acids (cis-MUFA), polyunsaturated fatty acids (PUFA), as well as unsaturated fatty acids (UFA) in the liver, heart, and especially in SF and PF (i.e. energy storage tissues) compared with the control group and SeVI-fed sheep. Dietary SeVI more effectively increased the capacity of ∆9-desaturation in all adipose tissues as the concentration of cis9,trans11CLA increased in these tissues. The addition of SeVI to the diet decreased the yield of the catabolism of fatty acids in these adipose tissues and both muscles compared with the control sheep or other experimental groups. The diets enriched with LO with or without SeVI increased the nutritional value of MBF, MLD, and adipose tissues as the ratio of unsaturated FA/saturated FA increased. Our results of a short-term study provide useful information for nutritionists carrying out further investigations to improve the nutritional quality of feed for ruminants as well as humans. &nbsp;

scholarly journals Enhancing unsaturated fatty acids in ewe's milk by feeding rapeseed or linseed oil

2010 ◽  
Vol 55 (No. 1) ◽  
pp. 496-504 ◽  
Author(s):  
A. Cieslak ◽  
J. Kowalczyk ◽  
M. Czauderna ◽  
A. Potkanski ◽  
M. Szumacher-Strabel
Keyword(s):  
Fatty Acids ◽  
Milk Fat ◽  
Rapeseed Oil ◽  
Unsaturated Fatty Acids ◽  
Linseed Oil ◽  
Fatty Acid Content ◽  
Latin Square ◽  
Feed Additives ◽  
Control Group ◽  
Experimental Conditions

The aim of our research was to evaluate whether rapeseed and linseed oils used as feed additives to ewe's diets allow to achieve the higher secretion of unsaturated fatty acids into milk with extended emphasis on the conjugated linoleic acid concentration. Two experiments were carried out on 6 lactating ewes in two independent 3 &times; 3 Latin square designs. In each feeding cycle the animals were subjected to one of the treatments: (1) without oil supplementation, (2) with 3.5% of rapeseed oil (RS) or linseed oil (LS), (3) with 7.0% of rapeseed oil or linseed oil. Feeding diets rich in mono- and polyunsaturated fatty acids to lactating ewes under our experimental conditions resulted in demanded changes in milk fatty acid content without causing milk fat depression or modification of other milk constituents. Milk fat was improved in unsaturated fatty acids including C18:2 c9 t11 CLA isomer without changing the milk fat concentration. The C18:2 c9 t11 concentration increased from 0.03 (FAME, %) in the control group to 0.21 and 0.12 (FAME, %) in groups receiving 3.5 and 7% RS, respectively. The diet with 7% LS also elevated the C18:2 c9 t11 level in milk from 0.04 (FAME, %) in the control group to 0.11 (FAME, %). Hence a conclusion is drawn that plant oils may be an important source of monoenic and also polyenic fatty acids in milk, however, our research and analysis of literature data indicate that results are strictly dietary and supplement-dependent.

scholarly journals Enhancing unsaturated fatty acids in ewe's milk by feeding rapeseed or linseed oil

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 496-504 ◽  
Author(s):  
A. Cieslak ◽  
J. Kowalczyk ◽  
M. Czauderna ◽  
A. Potkanski ◽  
M. Szumacher-Strabel
Keyword(s):  
Fatty Acids ◽  
Milk Fat ◽  
Rapeseed Oil ◽  
Unsaturated Fatty Acids ◽  
Linseed Oil ◽  
Fatty Acid Content ◽  
Latin Square ◽  
Feed Additives ◽  
Control Group ◽  
Experimental Conditions

The aim of our research was to evaluate whether rapeseed and linseed oils used as feed additives to ewe's diets allow to achieve the higher secretion of unsaturated fatty acids into milk with extended emphasis on the conjugated linoleic acid concentration. Two experiments were carried out on 6 lactating ewes in two independent 3 &times; 3 Latin square designs. In each feeding cycle the animals were subjected to one of the treatments: (1) without oil supplementation, (2) with 3.5% of rapeseed oil (RS) or linseed oil (LS), (3) with 7.0% of rapeseed oil or linseed oil. Feeding diets rich in mono- and polyunsaturated fatty acids to lactating ewes under our experimental conditions resulted in demanded changes in milk fatty acid content without causing milk fat depression or modification of other milk constituents. Milk fat was improved in unsaturated fatty acids including C18:2 c9 t11 CLA isomer without changing the milk fat concentration. The C18:2 c9 t11 concentration increased from 0.03 (FAME, %) in the control group to 0.21 and 0.12 (FAME, %) in groups receiving 3.5 and 7% RS, respectively. The diet with 7% LS also elevated the C18:2 c9 t11 level in milk from 0.04 (FAME, %) in the control group to 0.11 (FAME, %). Hence a conclusion is drawn that plant oils may be an important source of monoenic and also polyenic fatty acids in milk, however, our research and analysis of literature data indicate that results are strictly dietary and supplement-dependent.

scholarly journals Profile of ingested fatty acids and in the duodenal digest of steers fed different diets

2010 ◽  
Vol 39 (11) ◽  
pp. 2502-2511 ◽  
Author(s):  
Luís Fernando Glasenapp de Menezes ◽  
Gilberto Vilmar Kozloski ◽  
João Restle ◽  
Ivan Luiz Brondani ◽  
Raul Dirceu Pazdiora ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Latin Square ◽  
Tropical Pasture ◽  
Ruminal Fermentation ◽  
Omega 3 ◽  
Conventional Diet

It was evaluated in this study the effect of the type of the diet on duodenal flow of long-chain fatty acids in steers. The tested diets were the following: conventional (feedlot diet composed of 60% corn silage and 40% of concentrate); winter forage silage - rye grass (Lolium multiflorum, Lam); or tropical forage silage - association of millet (Pennisetum americanum, Leeke + alexander grass, Brachiaria plantaginea). Six Charolais × Nellore crossbred steers with cannulas in duodenum were used in a 3 × 3 double Latin square. Dry material intake was similar among the groups (mean of 4,037 g/day), but the intake of total fatty acids and saturated fatty acids were higher in the group fed tropical pasture silage. On the other hand, the animals which received the conventional diet consumed higher quantity of unsaturated fatty acids. Tropical pasture silage provided higher consumption of vacenic acid (C18:1 t-11) and the winter forage silage offered higher consumption of conjugated linoleic acid. The intake of omega-6 fatty acids was higher in the group fed conventional diet and for omega-3, intake was higher in the group fed tropical pasture diet. The total fatty acid flow in the duodenum was not affected by the diets, but in all treatments it was higher than the consumed one. The animals fed diet with concentrate show the greatest changes on the profile of fatty acids during the ruminal fermentation. Conventional diets provide the highest intake of unsaturated fatty acids and the highest availability of vacenic acid in the small intestine, but they do not increase the supply of intestinal conjugated linoleic acid.

Effect of Dietary Enrichment with Omega 3 and 6 Fatty Acids on Blood Metabolites, Hormone Concentration and Ovarian Function in Sahiwal Heifers

2021 ◽  
Author(s):  
Sushil Kumar ◽  
Sajjan Sihag ◽  
Zile Singh Sihag ◽  
Chandershekhar Santosh Patil ◽  
Surender Singh Dhaka ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Ovarian Function ◽  
Linseed Oil ◽  
Hdl Cholesterol ◽  
Blood Metabolites ◽  
Omega 3 ◽  
Positive Effects ◽  
Concentrate Mixture

Background: Supplementation of fat, especially those having unsaturated fatty acids has been proposed to carry positive effects on the reproductive organ, beside providing dietary energy to the animals. This experiment was designed to examine the effects of dietary ω-3 or ω-6 fatty acid (FA) rich oil supplementation on blood FA, metabolite and hormone concentrations; ovarian follicular growth and corpus luteum (CL) size in Sahiwal breed heifers.Methods: Eighteen heifers of 18.33±1.14 months of age and 194±4.16 kg of mean body weight were randomly assigned to 3 diets and individually fed as per ICAR (2013) diets. The diets include chopped wheat straw, green fodder and concentrate mixture containing either (i) no added PUFA rich oil but palm oil @ 3.5% (PO; n=6); (ii) 3.5% added soybean oil as ω-6 FA source (SO; n=6); or (iii) 3.5% added linseed oil as ω-3 FA source (LO; n=6).Result: SO increased (P less than 0.05) the plasma concentration of ω-6 FA while LO increased (P less than 0.05) the plasma ω-3 FA. Plasma glucose, triglyceride and non-esterified fatty acid (NEFA) concentrations was not affected due to different diets. Plasma total cholesterol and HDL-cholesterol were significantly higher (P less than 0.05) in SO and LO in comparison to PO. However, LDL-cholesterol was at par among all the treatments. Growth Hormone (GH) was not influenced due to different types of oil in heifers’ concentrate mixture. Insulin concentration increased (P less than 0.05) in LO compared to others. IGF-1 was statistically higher (P less than 0.05) in SO and LO as compared to CON, which among themselves also varied significantly. Plasma progesterone concentration at day 12 post estrous was higher (P less than 0.05) in LO. PUFA rich oil supplementation in the concentrate mixture of heifers (SO and LO) increased (P less than 0.05) the size of the ovulatory follicles as well as size of CL. It was inferred that feeding PUFA rich oil to pre-pubertal Sahiwal heifer’s results in advantageous changes in the blood metabolites, plasma hormones and ovarian functions.

The effect of lipid sources on intake, rumen fermentation parameters and microbial protein synthesis in Nellore steers supplemented with glycerol

2014 ◽  
Vol 54 (10) ◽  
pp. 1871 ◽  
Author(s):  
Arturo Samuel Gomez Insuasti ◽  
Yury Tatiana Granja Salcedo ◽  
Pablo de Souza Castagnino ◽  
Bruno Ramalho Vieira ◽  
Euclides Braga Malheiros ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Ether Extract ◽  
Microbial Protein ◽  
Molar Proportion ◽  
Fermentation Parameters

The effects of glycerol with fat sources as a feed alternative were investigated in the ration for Nellore steers (Bos indicus). Eight cannulated steers at 30 months of age with initial bodyweight of 554 ± 36.0 kg were used in a double 4 by 4 Latin square design with four consecutive 16-day periods. Treatments were three different diets with lipid sources (soybean, soybean oil and calcium salts of fatty acids) and one diet control without lipid sources. All diets formulated contained 10% crude glycerol and 5% ether extract with a forage:concentrate ratio of 35:65; corn silage was used as forage. The ruminal liquid was sampled for 24 h and ruminal fermentations were monitored by measuring pH, concentrations of ammonia nitrogenand volatile fatty acids in rumen fluid. Urine samples were obtained from the total collection for 24 h for estimation of rumen microbial protein supply using urinary purine derivatives. Our results showed that the use of lipid sources combined with glycerol did not induce significant changes in rumen pH, acetate molar proportion, ruminal microbial protein or dry matter intake. Although the acetate molar proportion was kept constant within normal parameters, the propionate molar proportion was increased by the diet containing lipid sources. Moreover, we found that there was a negative effect of lipid sources on crude protein and neutral detergent fibre corrected for ash and protein intake, and one positive effect on ether extract intake. We believe that association between fat and glycerol may affect rumen fermentation parameters through reducing fibre intake and increasing propionate production and ammonia nitrogen.

Effect of supplementation with Yerba Mate (Ilex paraguariensis) and vitamin E on milk lipoperoxidation in cows receiving diets containing ground soybean seeds

2019 ◽  
Vol 86 (3) ◽  
pp. 279-282
Author(s):  
Fabio Seiji Santos ◽  
Lucia Maria Zeoula ◽  
Luciano Soares De Lima ◽  
Francilaine Eloise De Marchi ◽  
Luís Carlos Vinhas Ítavo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Unsaturated Fatty Acids ◽  
Control Diet ◽  
Latin Square ◽  
Reducing Power ◽  
Dietary Vitamin ◽  
Ilex Paraguariensis ◽  
Soybean Seeds ◽  
Yerba Mate

AbstractThis research communication addresses the hypothesis that the association of dietary vitamin E and Yerba Mate could help to prevent or decrease oxidation of milk enriched in unsaturated fatty acids (UFA). Four multiparous lactating Holstein cows were used in a 4 × 4 Latin square. Treatments were: (1) control diet with no Yerba Mate or vitamin E; (2) diet containing 375 IU/kg vitamin E; (3) diet containing 30 g/kg Yerba Mate; and (4) diet containing 375 IU/kg vitamin E and 30 g/kg Yerba Mate. To increase unsaturated fatty acids in milk, cows were fed 172 g/kg soybean seeds (on a dry matter basis). There was no interaction between vitamin E and Yerba Mate supplementation for milk antioxidant-related (polyphenols, reducing power, conjugated dienes, and TBARS) analyses. Milk reducing power was increased when cows were supplemented with Yerba Mate. Our results suggest that the association of dietary vitamin E and Yerba Mate does not help to prevent or decrease oxidation of milk in UFA.

Effect of amount of rumen degradable protein on the utilization of wheat straw by Dohne Merino wethers

2003 ◽  
Vol 76 (2) ◽  
pp. 319-326
Author(s):  
J. van E. Nolte ◽  
A.V. Ferreirat ◽  
H.H. Köster
Keyword(s):  
Organic Matter ◽  
Wheat Straw ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Microbial Nitrogen ◽  
Digestible Organic Matter ◽  
Microbial Efficiency ◽  
Forage Intake ◽  
Degradable Protein

AbstractA 5 ✕ 5 Latin square was conducted with ruminally and duodenally cannulated Dohne Merino wethers consuming wheat straw to determine the effect of different rumen degradable protein (RDP) levels on forage intake, fermentation characteristics, nutrient flow and digestion. The wethers had ad libitum access to water and wheat straw (32 g crude protein (CP) per kg dry matter (DM); 742 g neutral-detergent fibre (NDF) per kg DM) that was offered twice daily, immediately after intraruminal infusion of the supplements at 07:00 and 19:00 h. The supplemental RDP (calcium caseinate; 900 g CP per kg DM) levels were: 0, 40, 80, 120 and 160 g/day. Each period consisted of 14 days of adaptation and 6 days of sampling. Forage and total organic matter (OM) intakes increased in a linear manner (P < 0•01) with increasing supplemental RDP levels. Digestible organic matter intake (DOMI) displayed a quadratic increase with elevated amounts of RDP (P < 0•01). The effects of treatments on rumen and total tract digestion, as well as fluid dilution rate were minimal. Microbial nitrogen (MN) flow to the duodenum and microbial efficiency increased quadratically (P < 0•04) with increased RDP supplementation. Rumen ammonia nitrogen (NH3-N) concentrations increased linearly (P < 0•01) and total volatile fatty acids (VFA) tended to increase linearly (P = 0•07). In conclusion, RDP supplementation to Dohne Merino wethers consuming wheat straw generally enhanced rumen fermentation and forage intake. A total RDP intake (sources: calcium caseinate and wheat straw) of 3•30 g/kg M0•75 or 0•12 of DOM maximized DOMI.

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