Isolation and characterization of endophytic Streptomyces sp. S5 with herbicidal activity from tomato roots

2006 ◽  
Vol 3 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Qiu Zhi-Qi ◽  
Cao Li-Xiang ◽  
Tan Hong-Ming ◽  
Zhou Shi-Ning

AbstractFifty-eight actinomycetes were isolated from surface-sterilized tomato (Lycopersicon esculentum) roots and 43 isolates were screened for herbicidal activities. Isolate S5 was found to have potent herbicidal activity against germination of wheat (Triticum aestivum L.), mung bean (Phaseolus radiatus L.) and grass (Paspalum notatum and Cynodon dactylon) seeds. But the metabolites of isolate S5 showed no influence on the growth of wheat seedlings. The S5 strain was identified as Streptomyces lavendulae var. glaucescens based on its morphological characteristics and physiological properties. The highest herbicidal activity was observed when 2% inocula were applied into S medium (containing 1% glucose, 0.3% beef extract and pH 7.0) and incubated at 25°C on a rotary shaker (160 rpm).

2020 ◽  
Vol 9 (2) ◽  
pp. 86-90
Author(s):  
Maria V. Mantrova

The paper deals with ecological and physiological properties of four strains of the species Penicillium chrysogenum Thom 1910, taken on the territory of Surgut from soil, water and the surface of synthetic polymers, depending on the source of carbon nutrition and temperature. The author also considers phytotoxic properties of these strains in relation to several test objects. The cultural and morphological characteristics of the strains are similar to each other in places with sucrose, lactose, mannitol, starch and cellulose, and are variable in places with sorbitol and glycerol. The highest average values of the radial growth rate were recorded in places with alcohols sorbitol and glycerine. General cultural and morphological characteristics are typical for strains when cultivated in the same temperature regime; a low temperature of +5C contributes more to the growth of colonies than a high temperature of +37C, the optimal one is +25C; the obtained data confirm that P. chrysogenum belongs to mesophilic (psychrotolerant) species. All strains are toxic to radish seedlings; variably toxic to wheat seedlings, Canada water weed and duckweed and non-toxic to wheat and radish seeds. The obtained results can be used for studying the ecology of P. chrysogenum strains, as well as for identifying this species.


Author(s):  
J. E. M. Mordue

Abstract A description is provided for Sclerotinia homoeocarpa. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On turf grasses: Agrostis alba, A. canina, A. palustris, A. stolonifera, A. stolonifera subsp. compacta, A. tenuis, Avena, Cynodon dactylon, Digitaria didacryla, Festuca ovina, F. rubra, F. rubra subsp. comutata, F. rubra var. fallax, Holcus lanatus, Lolium, Paspalum notatum, Pennisetum clandestinum, Poa annua, P. pratensis, P. trivialis, Puccinellia maritima, Cyperus rotundus. By inoculation on radish, lettuce, beet, tomato, subterranean clover, pea, bean and wheat seedlings, barley, onion. DISEASE: Dollar spot of turf. The spots are about 5 cm diam. and approximately circular, though they sometimes coalesce to form irregular patches. They are brown at first, later becoming bleached to straw coloured. The fungus is present on leaves, stems and basal parts of the plants, which eventually die. GEOGRAPHICAL DISTIBUTION: Britain and Northern Ireland, Netherlands, Australia, New Zealand (57, 4372), USA. TRANSMISSION: No detailed studies made. The fungus is known to tee capable of overwintering in individual dollar spots (40, 689). Dissemination by infected leaf fragments has been reported (Smith, 1955).


2014 ◽  
Vol 955-959 ◽  
pp. 235-238 ◽  
Author(s):  
Dan Dan Li ◽  
Yong Ze Yuan ◽  
Yuan Lei Chen ◽  
Na Li ◽  
Jing Long Li ◽  
...  

In this study, three bacterium strains capable of degrading methamidophos (MAP) were isolated from the soil samples contaminated with organophosphorus pesticides (OPs). According to morphological characteristics, physiological properties, and 16S rDNA sequence analysis, the isolates HS-A32, HS-D36, and HS-D38 were identified asAcinetobacter sp.,Pseudomonas stutzeriandPseudomonas aeruginosa, respectively. They could utilize MAP as the sole carbon and nitrogen source, and the degradation ratio upon 500 mg/L MAP could reach 80% in 3 days under optimal conditions (pH 7.0, 30-35 °C). The strains could use glucose, fructose, ethanol, and galactose as carbon and energy sources, and the favorable nitrogen source included organic and inorganic nitrogen. Further studies showed that the three strains could degrade various OPs. The characterized strains with broad-spectrum OP-degrading activities could be useful for the biodegradation of MAP and the other OPs.


Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 144-151
Author(s):  
P. Vijayalakshmi ◽  
A. Rajani Chowdary ◽  
P. Vidyullatha ◽  
M. Sharon Sushma

The current study aimed to isolate bacteria that harbour various animal food products like meat, chicken and seafoods collected from the abattoir, butcher shops and local seafood market and to determine the antimicrobial resistance pattern of isolated pathogens which are responsible for various foodborne illnesses in human beings. A total of forty raw animal product samples were collected from the abattoir, butcher shops and local seafood market of Visakhapatnam. The samples selected for the study include raw chicken, meat, crab, prawns and different varieties of fish. A classic random sampling technique was employed to collect the study samples. All the samples were processed immediately using standard microbiological protocols. The bacteria isolation and characterization were done by studying morphological characteristics with staining methods, cultural characteristics by isolating and growing the pathogenic microorganisms in various selective and differential culture media. Antimicrobial susceptibility testing was performed by the Kirby -Bauer method by following Clinical and Laboratory Standards Institute (CLSI) guidelines. EDTA-Disc Potentiation Test and Imipenem-EDTA Double disc synergy test are used to detect the metallo beta-lactamase production of isolated pathogens. The highest number of isolates belong to Salmonella species (18), Pseudomonas aeruginosa (18) followed by Vibrio species (14) and few isolates belong to Enterobacter species (4). Majority of the microbial isolates obtained in the current study were multidrug resistant. The isolates from the abattoir environments, slaughterhouses, fish markets were found to exhibit variable resistance pattern to aminoglycosides, macrolides, β-lactams, cephalosporins, quinolone antibiotics used in the present study and at the same time most of them were sensitive to carbapenem antibiotic imipenem. Antimicrobial resistance (AMR) prevents the designing and assessment of effective interventions. If such a link can be established, then the tracking of antibiotic use and consumption data could be furthermore used as a surrogate indicator for the risk of potential antibiotic resistance (ABR) emergence.


2018 ◽  
Vol 6 (1) ◽  
pp. 12-16
Author(s):  
Chalinda Koshitha Beneragama ◽  
Ganege Don Kapila Kumara

Drought responses of turf-type Bermuda grass (Cynodon dactylon (L.) Pers.) and Bahia grass (Paspalum notatum Flugge) in relation to their growth, carbon allocation and accumulation of nonstructural carbohydrates were investigated. Seedlings were exposed to a 20 day terminal drought, followed by 20 day recovery with irrigation. Leaf dry matter was lower in water-stressed plants than control plants by the end of drought, but did not differ in roots. For both species, more carbon was allocated towards roots in response to drought. Total nonstructural carbohydrate (TNC) concentration in shoots was increased in drought-stressed plants of both species. However, root TNC concentrations in Bahia grass were decreased after drought. TNC pool sizes also showed a similar variation, hence correlated with TNC concentrations. Bahia grass maintained a better quality than Bermuda grass after the drought, having recorded a higher aesthetic score, higher relative water content and a lower electrolyte leakage. Results suggest that, both species are considerably tolerant to short-term drought though Bahia grass seems more promising.Int. J. Appl. Sci. Biotechnol. Vol 6(1): 12-16


Blood ◽  
1984 ◽  
Vol 63 (3) ◽  
pp. 510-517 ◽  
Author(s):  
IL Olsson ◽  
MG Sarngadharan ◽  
TR Breitman ◽  
RC Gallo

Abstract Mitogen-stimulated mononuclear blood cells produce differentiation inducing factors (DIFs) for the promyelocytic cell line HL-60. We report that DIF is produced constitutively by a malignant T lymphocyte line HUT-102. DIF was purified 7,000-fold from HUT-102 conditioned media by utilizing ion-exchange chromatography with DEAE-Sepharose, gel chromatography, Blue-Sepharose chromatography, and preparative SDS- polyacrylamide gel electrophoresis (SDS-PAGE). The final preparation is susceptible to protease treatment, has a molecular weight of 46,000, as determined by SDS-PAGE and approximately 55,000 by gel filtration, has an isoelectric point of approximately 5.2, does not adhere to lectin- Sepharose and is resistant to periodate oxidation, and is free of colony-stimulating factor. DIF induced maturation of HL-60 into phagocytizing nitro blue tetrazolium reducing cells with the morphological characteristics of myelomonocytic or monocyte-like cells. An activity, co-chromatographing with DIF, acts synergistically with retinoic acid to induce maturation not only of HL-60, but also of the monoblast-like cell line U-937 (measured as percentage of cells reducing NBT).


2006 ◽  
Vol 46 (9) ◽  
pp. 1217 ◽  
Author(s):  
C. M. Menzel ◽  
P. Broomhall

The effects of fertilisers on 8 tropical turfgrasses growing in 100-L bags of sand were studied over winter in Murrumba Downs, just north of Brisbane in southern Queensland (latitude 27.4°S, longitude 153.1°E). The species used were: Axonopus compressus (broad-leaf carpetgrass), Cynodon dactylon (bermudagrass ‘Winter Green’) and C. dactylon × C. transvaalensis hybrid (‘Tifgreen’), Digitaria didactyla (Queensland blue couch), Paspalum notatum (bahiagrass ‘38824’), Stenotaphrum secundatum (buffalograss ‘Palmetto’), Eremochloa ophiuroides (centipedegrass ‘Centec’) and Zoysia japonica (zoysiagrass ‘ZT-11’). Control plots were fertilised with complete fertilisers every month from May to September (72 kg N/ha, 31 kg P/ha, 84 kg K/ha, 48 kg S/ha, 30 kg Ca/ha and 7.2 kg Mg/ha), and unfertilised plots received no fertiliser. Carpetgrass and standard bermudagrass were the most sensitive species to nutrient supply, with lower shoot dry weights in the unfertilised plots (shoots mowed to thatch level) compared with the fertilised plots in June. There were lower shoot dry weights in the unfertilised plots in July for all species, except for buffalograss, centipedegrass and zoysiagrass, and lower shoot dry weights in the unfertilised plots in August for all species, except for centipedegrass. At the end of the experiment in September, unfertilised plots were 11% of the shoot dry weights of fertilised plots, with all species affected. Mean shoot nitrogen concentrations fell from 3.2 to 1.7% in the unfertilised plots from May to August, below the sufficiency range for turfgrasses (2.8–3.5%). There were also declines in P (0.45–0.36%), K (2.4–1.5%), S (0.35–0.25%), Mg (0.24–0.18%) and B (9–6 mg/kg), which were all in the sufficiency range. The shoots in the control plots took up the following levels (kg/ha.month) of nutrients: N, 10.0–27.0; P, 1.6–4.0; K, 8.2–19.8; S, 1.0–4.2; Ca, 1.1–3.3; and Mg, 0.8–2.2, compared with applications (kg/ha.month) of: N, 72; P, 31; K, 84; S, 48; Ca, 30; and Mg, 7.2, indicating a recovery of 14–38% for N, 5–13% for P, 10–24% for K, 2–9% for S, 4–11% for Ca and 11–30% for Mg. These results suggest that buffalograss, centipedegrass and zoysiagrass are less sensitive to low nutrient supply than carpetgrass, bermudagrass, blue couch and bahiagrass. Data on nutrient uptake showed that the less sensitive species required only half or less of the nitrogen required to maintain the growth of the other grasses, indicating potential savings for turf managers in fertiliser costs and the environment in terms of nutrients entering waterways.


2017 ◽  
Vol 2 (47) ◽  
pp. 13-30
Author(s):  
Silvia Irene Boccanelli ◽  
Claudia Alzugaray ◽  
Eduardo Andrés Franceschi

El objetivo de este trabajo fue caracterizar las comunidades herbáceas espontáneas que constituyen los céspedes del parque J.F. Villarino (Provincia de Santa Fe, Argentina) y evaluar la vegetación emergente y el banco de semillas del suelo. Se utilizaron parcelas de inventario de 16 m 2 . Los datos fueron analizados con métodos multivariados. En la vegetación se observaron tres grupos: Grupos 1, 2 y 3 dominados por Cynodon dactylon, Paspalum notatum y Digitaria sanguinalis, respectivamente. En el banco de semillas se reconocieron dos grupos, que se diferenciaron por la importancia relativa de alguna de sus especies. El 62 % de las especies del banco presentó una distribución agregada (Índice de Morisita). La similitud entre la vegetación y el banco fue de 55,7% (Índice de Sörensen). El 47 % de las especies que en la vegetación se destacaron por su constancia y/o abundancia, también se encontraron en el banco, aunque pocas alcanzaron una densidad alta. En ambas situaciones predominaron las especies nativas (80,3 % en céspedes y 61,9 % en el banco del suelo) y perennes (73,8 % y 58,5 % respectivamente). Los resultados señalan la importancia de preservar los céspedes espontáneos, dado que contienen y actúan como refugio de especies nativas del pastizal pampeano extinto en la región. 


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