A shotgun proteomic approach reveals novel potential salivary protein biomarkers for asthma

The diagnosis of pediatric multiple sclerosis (MS) is challenging due to its low frequency and the overlap with other acquired childhood demyelinating disorders of the central nervous system. To identify potential protein biomarkers which could facilitate the diagnosis, we used two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry to identify proteins associated with pediatric MS. Plasma samples from nine children with MS and nine healthy subjects, matched in aggregate by age and gender, were analyzed for differences in their patterns of protein expression. We found 12 proteins that were significantly up regulated in the pediatric MS group: alpha-1-acid-glycoprotein 1, alpha-1-B-glycoprotein, transthyretin, apoliprotein-C-III, serum amyloid P component, complement factor-I, clusterin, gelsolin, hemopexin, kininogen-1, hCG1993037-isoform, and vitamin D-binding protein. These results show that 2-DE in combination with mass spectrometry is a highly sensitive technique for the identification of blood-based biomarkers. This proteomic approach could lead to a new panel of diagnostic and prognostic markers in pediatric MS.

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Identification of Salivary Protein Biomarkers ( IL-1RA and SLPI ) ; Matrix Metalloproteinase ( MMP-2 and MMP-9 ) and IL-6 Levels in Type 1 Diabetic Patients in Relation to HbA1c in Iraqi Population

International Journal for Sciences and Technology ◽

10.12816/0023799 ◽

2016 ◽

Vol 11 (1) ◽

pp. 29-34

Author(s):

Rehab F. Ahmed ◽

Raja H. A. Al-Jubouri ◽

Ahmed S. Alnuaimi

Keyword(s):

Matrix Metalloproteinase ◽

Salivary Protein ◽

Diabetic Patients ◽

Protein Biomarkers ◽

Type 1 Diabetic Patients

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A Proteomic Approach for the Diagnosis of‘Oketsu’(blood stasis), a Pathophysiologic Concept of Japanese Traditional (Kampo) Medicine

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem049 ◽

2008 ◽

Vol 5 (4) ◽

pp. 463-474 ◽

Cited By ~ 40

Author(s):

Chinami Matsumoto ◽

Tetsuko Kojima ◽

Kazuo Ogawa ◽

Satoshi Kamegai ◽

Takuya Oyama ◽

...

Keyword(s):

Decision Tree ◽

Hierarchical Clustering ◽

Blood Stasis ◽

Minor Component ◽

Principal Component ◽

Diagnostic Algorithm ◽

Protein Profiles ◽

Blood Proteins ◽

Protein Biomarkers ◽

Proteomic Approach

‘Oketsu’is a pathophysiologic concept in Japanese traditional (Kampo) medicine, primarily denoting blood stasis/stagnant syndrome. Here we have explored plasma protein biomarkers and/or diagnostic algorithms for‘Oketsu’. Sixteen rheumatoid arthritis (RA) patients were treated withkeishibukuryogan(KBG), a representativeKampomedicine for improving‘Oketsu’. Plasma samples were diagnosed as either having an‘Oketsu’(n= 19) or ‘non-Oketsu’ (n= 29) state according to Terasawa's‘Oketsu’scoring system. Protein profiles were obtained by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) and hierarchical clustering and decision tree analyses were performed. KBG treatment for 4 or 12 weeks decreased the‘Oketsu’scores significantly. SELDI protein profiles gave 266 protein peaks, whose expression was significantly different between the‘Oketsu’and ‘non-Oketsu’ states. Hierarchical clustering gave three major clusters (I, II, III). The majority (68.4%) of‘Oketsu’samples were clustered into one cluster as the principal component of cluster I. The remaining‘Oketsu’profiles constituted a minor component of cluster II and were all derived from patients cured of the‘Oketsu’state at 12 weeks. Construction of the decision tree addressed the possibility of developing a diagnostic algorithm for‘Oketsu’. A reduction in measurement/pre-processing conditions (from 55 to 16) gave a similar outcome in the clustering and decision tree analyses. The present study suggests that the pathophysiologic concept ofKampomedicine‘Oketsu’has a physical basis in terms of the profile of blood proteins. It may be possible to establish a set of objective criteria for diagnosing‘Oketsu’using a combination of proteomic and bioinformatics-based classification methods.

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Alterations in Salivary Proteome following Single Twenty-Minute Session of Yogic Breathing

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/376029 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Sundaravadivel Balasubramanian ◽

Michael G. Janech ◽

Graham W. Warren

Keyword(s):

Mass Spectrometry ◽

Western Blotting ◽

Cognitive Processes ◽

Significant Interaction ◽

Molecular Level ◽

Salivary Protein ◽

Single Session ◽

Protein Biomarkers ◽

Yogic Breathing ◽

Minute Session

Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes. However, alterations after YB at the molecular level are not well established. Twenty healthy volunteers were randomized into two groups (N=10per group): YB or attention controls (AC). The YB group performed two YB exercises, each for ten minutes, for a total of twenty minutes in a single session. AC group read a text of their choice for 20 minutes. Saliva was collected at baseline and at 5, 10, 15, and 20 minutes. Using Mass Spectrometry (MS), we initially found that 22 proteins were differentially expressed and then validated deleted in malignant brain tumor-1 (DMBT1) and Ig lambda-2 chain C region (IGLC2) using Western Blotting. DMBT1 was elevated in 7 of YB group by 10-fold and 11-fold at 10 and 15 minutes, respectively, whereas it was undetectable in the time-matched AC group (P<0.05). There was a significant interaction between groups and time assessed by two-way ANOVA (P<0.001). IGLC2 also showed a significant increase in YB group as measured by Western Blotting. These data are the first to demonstrate the feasibility of stimulating and detecting salivary protein biomarkers in response to an acute Yoga exercise. This trial is registered with ClincalTrials.govNCT02108769.

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Discovery of potential protein biomarkers associated with sugarcane white leaf disease susceptibility using a comparative proteomic approach

PeerJ ◽

10.7717/peerj.12740 ◽

2022 ◽

Vol 10 ◽

pp. e12740

Author(s):

Kantinan Leetanasaksakul ◽

Sittiruk Roytrakul ◽

Narumon Phaonakrop ◽

Suthathip Kittisenachai ◽

Siriwan Thaisakun ◽

...

Keyword(s):

Metal Ion ◽

Defense Mechanism ◽

Potential Candidate ◽

Protein Biomarkers ◽

Sugarcane Varieties ◽

Leaf Disease ◽

Proteomic Approach ◽

White Leaf ◽

Sugarcane White Leaf ◽

White Leaf Disease

Sugarcane white leaf disease (SCWLD) is caused by phytoplasma, a serious sugarcane phytoplasma pathogen, which causes significant decreases in crop yield and sugar quality. The identification of proteins involved in the defense mechanism against SCWLD phytoplasma may help towards the development of varieties resistant to SCWLD. We investigated the proteomes of four sugarcane varieties with different levels of susceptibility to SCWLD phytoplasma infection, namely K88-92 and K95-84 (high), KK3 (moderate), and UT1 (low) by quantitative label-free nano-liquid chromatography-tandem mass spectrometry (nano LC-MS/MS). A total of 248 proteins were identified and compared among the four sugarcane varieties. Two potential candidate protein biomarkers for reduced susceptibility to SCWLD phytoplasma were identified as proteins detected only in UT1. The functions of these proteins are associated with protein folding, metal ion binding, and oxidoreductase. The candidate biomarkers could be useful for further study of the sugarcane defense mechanism against SCWLD phytoplasma, and in molecular and conventional breeding strategies for variety improvement.

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A Differential Proteomic Approach to Characterize the Cell Wall Adaptive Response to CO2 Overpressure during Sparkling Wine-Making Process

Microorganisms ◽

10.3390/microorganisms8081188 ◽

2020 ◽

Vol 8 (8) ◽

pp. 1188

Author(s):

Juan Antonio Porras-Agüera ◽

Juan Carlos Mauricio ◽

Jaime Moreno-García ◽

Juan Moreno ◽

Teresa García-Martínez

Keyword(s):

Cell Wall ◽

Adaptive Response ◽

Wine Yeast ◽

Sparkling Wine ◽

Cell Wall Proteins ◽

Protein Biomarkers ◽

Wine Making ◽

Proteomic Approach ◽

Flor Yeast ◽

Related Proteins

In this study, a first proteomic approach was carried out to characterize the adaptive response of cell wall-related proteins to endogenous CO2 overpressure, which is typical of second fermentation conditions, in two wine Saccharomyces cerevisiae strains (P29, a conventional second fermentation strain, and G1, a flor yeast strain implicated in sherry wine making). The results showed a high number of cell wall proteins in flor yeast G1 under pressure, highlighting content at the first month of aging. The cell wall proteomic response to pressure in flor yeast G1 was characterized by an increase in both the number and content of cell wall proteins involved in glucan remodeling and mannoproteins. On the other hand, cell wall proteins responsible for glucan assembly, cell adhesion, and lipid metabolism stood out in P29. Over-represented proteins under pressure were involved in cell wall integrity (Ecm33p and Pst1p), protein folding (Ssa1p and Ssa2p), and glucan remodeling (Exg2p and Scw4p). Flocculation-related proteins were not identified under pressure conditions. The use of flor yeasts for sparkling wine elaboration and improvement is proposed. Further research based on the genetic engineering of wine yeast using those genes from protein biomarkers under pressure alongside the second fermentation in bottle is required to achieve improvements.

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Identification of salivary protein biomarkers for orthodontically induced inflammatory root resorption

PROTEOMICS - CLINICAL APPLICATIONS ◽

10.1002/prca.201600119 ◽

2017 ◽

Vol 11 (9-10) ◽

pp. 1600119 ◽

Cited By ~ 7

Author(s):

Karolina Elżbieta Kaczor-Urbanowicz ◽

Omer Deutsch ◽

Batia Zaks ◽

Guy Krief ◽

Stella Chaushu ◽

...

Keyword(s):

Root Resorption ◽

Salivary Protein ◽

Protein Biomarkers

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Putative salivary protein biomarkers for the diagnosis of oral lichen planus: a case-control study

BMC Oral Health ◽

10.1186/s12903-018-0504-8 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 5

Author(s):

Sineepat Talungchit ◽

Waranun Buajeeb ◽

Chotima Lerdtripop ◽

Rudee Surarit ◽

Kongthawat Chairatvit ◽

...

Keyword(s):

Lichen Planus ◽

Oral Lichen Planus ◽

Case Control Study ◽

Case Control ◽

Salivary Protein ◽

Protein Biomarkers ◽

Oral Lichen ◽

Control Study

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Childhood Allergy Disease, Early Diagnosis, and the Potential of Salivary Protein Biomarkers

Mediators of Inflammation ◽

10.1155/2021/9198249 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

N. H. M. Zainal ◽

R. Abas ◽

S. F. Mohamad Asri

Keyword(s):

Allergic Disease ◽

Biological Samples ◽

Biological Fluid ◽

Genetic Material ◽

Food Challenge ◽

Salivary Protein ◽

Detection Methods ◽

Protein Biomarkers ◽

Prick Test ◽

Targeted Interventions

Allergic disease has risen to epidemic proportions since the last decade and is among the most common noncommunicable, chronic diseases in children and adolescents worldwide. Allergic disease usually occurs in early life; thus, early biomarkers of allergic susceptibility are required for preventive measures to high-risk infants which enable early interventions to decrease allergic severity. However, to date, there is no reliable general or specific allergy phenotype detection method that is easy and noninvasive for children. Most reported allergic phenotype detection methods are invasive, such as the skin prick test (SPT), oral food challenge (OFC), and blood test, and many involve not readily accessible biological samples, such as cord blood (CB), maternal blood, or newborn vernix. Saliva is a biological sample that has great potential as a biomarker measurement as it consists of an abundance of biomarkers, such as genetic material and proteins. It is easily accessible, noninvasive, collected via a painless procedure, and an easy bedside screening for real-time measurement of the ongoing human physiological system. All these advantages emphasise saliva as a very promising diagnostic candidate for the detection and monitoring of disease biomarkers, especially in children. Furthermore, protein biomarkers have the advantages as modifiable influencing factors rather than genetic and epigenetic factors that are mostly nonmodifiable factors for allergic disease susceptibility in childhood. Saliva has great potential to replace serum as a biological fluid biomarker in diagnosing clinical allergy. However, to date, saliva is not considered as an established medically acceptable biomarker. This review considers whether the saliva could be suitable biological samples for early detection of allergic risk. Such tools may be used as justification for targeted interventions in early childhood for disease prevention and assisting in reducing morbidity and mortality caused by childhood allergy.

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