Beauveria bassiana PSUB01 simultaneously displays biocontrol activity against Lipaphis erysimi (Kalt.) (Hemiptera: Aphididae) and promotes plant growth in Chinese kale under hydroponic growing conditions

Plant growth promoting rhizobacteria (PGPR) can be functional microbial fertilizers and/or biological control agents, contributing to an eco-spirit and safe solution for chemical replacement. Therefore, we have isolated rhizospheric arylsulfatase (ARS)-producing bacteria, belonging to Pseudomonas and Bacillus genus, from durum wheat crop grown on calcareous soil. These isolates harbouring plant growth promoting (PGP) traits were further evaluated in vitro for additional PGP traits, including indole compounds production and biocontrol activity against phytopathogens, limiting the group of multi-trait strains to eight. The selected bacterial strains were further evaluated for PGP attributes associated with biofilm formation, compatibility, salt tolerance ability and effect on plant growth. In vitro studies demonstrated that the multi-trait isolates, Bacillus (1.SG.7, 5.SG.3) and Pseudomonas (2.SG.20, 2.C.19) strains, enhanced the lateral roots abundance and shoots biomass, mitigated salinity stress, suggesting the utility of beneficial ARS-producing bacteria as potential microbial fertilizers. Furthermore, in vitro studies demonstrated that compatible combinations of multi-trait isolates, Bacillus sp. 1.SG.7 in a mixture coupled with 5.SG.3, and 2.C.19 with 5.SG.3 belonging to Bacillus and Pseudomonas, respectively, may enhance plant growth as compared to single inoculants.

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Sweet pepper seed responses to inoculation with microorganisms and coating with micronutrients, aminoacids and plant growth regulators

Scientia Agricola ◽

10.1590/s0103-90162009000300002 ◽

2009 ◽

Vol 66 (3) ◽

pp. 293-297 ◽

Cited By ~ 5

Author(s):

Kênia Almeida Diniz ◽

Paulo de Albuquerque Silva ◽

João Almir Oliveira ◽

José Renato Emiliorelli Evangelista

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Beauveria Bassiana ◽

Growth Regulators ◽

Metarhizium Anisopliae ◽

Trichoderma Viride ◽

Sweet Pepper ◽

Seedling Development ◽

Initial Development ◽

Antagonistic Microorganisms

Small sized seeds, such as the horticultural species, have limited quantities of reserves that can be balanced by coating then with essential nutrients for their initial development. In addition, inoculation of the seeds with microorganisms may protect the plants against phytopathogens, thus enhancing their growth. The present work had the objective of evaluate the physiological quality and seedling development of sweet pepper seeds and seedlings coated with several kind of films. Seeds were first coated with polymers and then with antagonistic microorganisms (Trichoderma viride, Trichoderma polysporhum, Trichoderma stromaticum, Beauveria bassiana, Metarhizium anisopliae), mycorrhizas, aminoacids, micronutrients and plant growth regulators. Evaluation was performed for percentage of germination and for seedling emergence, speed of emergence index, number of plants, dry mass of the aerial and root parts and height of the seedlings. Inoculation with Trichoderma viride increased the percentage and rate of the seedlings emergence Inoculation with Trichoderma viride, Metarhizium anisopliae and mycorrhizas promote better seedling development; seed microbiolization with microorganisms Trichoderma viride, T. polysporhum, T. stromaticum, Beauveria bassiana, Metarhizium anisopliae. Mycorrhizas mixture negatively affected seeds and seedling quality. Seed covering with plant growht regulator, at a 5 mL kg-1 dose increased the roots dry matter.

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Evaluación de la actividad biocontroladora de Beauveria bassiana y Metarhizium anisopliae sobre larvas de Ancognatha scarabaeiodes (Coleoptera: Scarabaeidae)

Ciencia y Tecnología Agropecuaria ◽

10.21930/rcta.vol5_num1_art:23 ◽

2004 ◽

Vol 5 (1) ◽

pp. 43

Author(s):

Any Mercedes Lucero Mafla ◽

Luis Alberto Peña Villamil ◽

Tito Bacca Ibarra

Keyword(s):

Beauveria Bassiana ◽

Metarhizium Anisopliae ◽

Crop Yields ◽

Integrated Management ◽

White Grubs ◽

Promising Alternative ◽

Fungal Entomopathogens ◽

Biocontrol Activity ◽

Mortality Percentage ◽

Lethal Doses

Actualmente, en los municipios del departamento de Nariño (Colombia), en los cultivos de trigo y papa que son los principales sistemas de producción, se presentan altas infestaciones de poblaciones de chisas encontrándose hasta 350 larvas/m2, representando un factor limitante para la producción de los cultivos. Una alternativa promisoria para el manejo de esta plaga es la utilización de métodos biológicos que incluyen el uso de hongos entomopatógenos. El objetivo del presente trabajo fue el de evaluar el efecto de Beauveria bassiana, Metarhizium anisopliae sobre larvas de Ancognatha scarabaeoides en laboratorio e invernadero. Los aislamientos de hongos se obtuvieron de larvas infectadas en los municipios de Yacuanquer y Ospina. En el laboratorio, se seleccionaron 5 de 9 aislamientos de hongos: Mt1, Bb cosmo, Mt2, Bb4 y Bb10 que presentaron mortalidades superiores al 50%, éstas se evaluaron a concentraciones de 1x105 a 1x1010 esporas/ml. El porcentaje acumulado de mortalidad a los 18 días después de la inoculación fueron: del 100% para Bb cosmo, Mt1 y Mt2 en la concentración de 1x1010 esporas/ml y del 90% para Bb4 y Bb10 en la concentración de 1x109 esporas/ml, presentando concentraciones letales 90(CL90) de 1.1x1010, 7.3x109, 2.2x1010, 3.9x1010 y 4.3x1010 esporas/ml respectivamente. En invernadero se evaluaron los mismos microorganismos con sus respectivas concentraciones letales 50(CL50) y 90(CL90), encontrándose porcentajes de mortalidad del 76.00%, 67.50%, 63.50%, 59.00% y 53.50% para Mt1, Bb cosmo, Mt2, Bb4 y Bb10. Estos resultados indican la posibilidad de incluir estos biocontroladores nativos en el manejo integrado de las chisas en Nariño. Evaluation of the biocontrol activity of Beauveria bassiana, Metarhizium anisopliae, on larvae of Ancognatha scarabaeoides (Coleoptera: Scarabaeidae).High grub populations of coleopteran, frequently attack crops of wheat and potato in the Department of Nariño, Colombia. Reports indicate that infestations levels reach more than 350 larvae/ m2, which severely reduce crop yields. Use of biological control measurements as fungal entomopathogens seems to be a promising alternative for management of this pest. The present work aimed to evaluate the effect of Beauveria bassiana and Metarhizium anisopliae on larvae of Ancognatha scarabaeoides both in laboratory and greenhouse conditions. Isolates of entomopathogen fungus were obtained from infected larvae in the municipalities of Yacuanquer and Ospina. A sample of 5 isolates, which previously had shown to cause mortality rates higher than 50%, was selected in the laboratory: Mt1, Bb cosmo, Mt2, Bb4 and Bb10. Isolates concentrations from 1x105 to 1x1010 spores/ml were tested. The cumulative mortality percentage (at 18 days after the inoculation) ranged from 100% with Bb cosmo, Mt1 and Mt2 at concentrations of 1x1010 spores/ml to 90% with Bb4 and Bb10 at concentrations of 1x109 spores/ml. Lethal doses 90 (LD90) of 1.1x1010, 7.3x109, 2.2x1010, 3.9x1010 and 4.3x1010 spores/ml were calculated for each isolate, respectively. An evaluation of the biocontrol activity of the isolates was carried out under greenhouse conditions. Mortality percentages obtained reached 76%, 67.5%, 63.5%, 59% and 53.5% for Mt1, Bb cosmo, Mt2, Bb4 and Bb10, respectively. These results suggest that the use of native biocontrolers is a promising alternative to be included in the integrated management for white grubs in Nariño.

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Selection of Endophytic Beauveria bassiana as a Dual Biocontrol Agent of Tomato Pathogens and Pests

Pathogens ◽

10.3390/pathogens10101242 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1242

Author(s):

Martina Sinno ◽

Marta Ranesi ◽

Ilaria Di Lelio ◽

Giuseppina Iacomino ◽

Andrea Becchimanzi ◽

...

Keyword(s):

Plant Growth ◽

Beauveria Bassiana ◽

Growth Promotion ◽

Protective Efficacy ◽

Good Control ◽

Integrate Pest Management ◽

Macrosiphum Euphorbiae ◽

Control Activity ◽

Commercial Strain ◽

Selection Of

Endophytic fungi (EF) can enhance both plant growth and defense barriers against pests and pathogens, contributing to the reduction of chemical pesticides and fertilizers use in agriculture. Beauveria bassiana is an entomopathogenic fungus showing endophytism in several crops, often associated with a good capacity to limit the development of pests and disease agents. However, the diversity of the protective efficacy and plant response to different strains can be remarkable and needs to be carefully assessed for the successful and predictable use of these beneficial microorganisms. This study aims to select B. bassiana strains able to colonize tomato plants as endophytes as well as to control two important disease agents, Botrytis cinerea and Alternaria alternata, and the pest aphid, Macrosiphum euphorbiae. Nine wild-type isolates and one commercial strain were screened for endophytism, then further characterized for plant-growth promotion plus inhibition of disease development and pest infestation. Four isolates proved to have a good control activity against the biotic stressors tested, but only Bb716 was also able to promote plant growth. This work provides a simple workflow for the selection of beneficial EF, paving the way towards more effective use of B. bassiana in Integrate Pest Management (IPM) of tomato.

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Scanning electron microscopic studies of Beauveria bassiana against Lipaphis erysimi Kalt

Journal of Applied and Natural Science ◽

10.31018/jans.v9i1.1214 ◽

2017 ◽

Vol 9 (1) ◽

pp. 461-465

Author(s):

Tanvi Sharma ◽

Neelam Joshi ◽

Anu Kalia

Keyword(s):

Beauveria Bassiana ◽

Lipaphis Erysimi ◽

Electron Microscopic ◽

Time Intervals ◽

Mustard Aphid ◽

Commercial Formulation ◽

Fungal Isolates ◽

Scanning Electron Microscopic ◽

Microscopic Studies ◽

Scanning Electron

This work was aimed to identify the LC50 of the indigenous fungal isolates for controlling L. erysimi infesta-tion in mustard aphid besides to probe the mechanism of action of the local isolates and comparison of the efficacy with the reference culture and commercial formulation ‘Mycojaal’. Three isolates of entomopathogenic fungi Beauveria bassiana were tested for infection on nymph of Lipaphis erysimi Kalt. using scanning electron microscopy (SEM) to record any variation. The SEM revealed adhesion of spores of B.bassiana followed by penetration of L.erysimi nymph surface. It was observed that all Beauveria isolates showed little variation with respect to penetration and adhesion at different time intervals. Further, lethal concentration (LC50) values of B. bassiana isolates against L. erysimi was recorded and was lowest (0.05x107 spores/ml) in B. bassiana MTCC 4495 and highest (0.11.X107 spores/ml) was recorded in native isolate F10 after 120 hours of treatment. The study has established the need for the isolation and evaluation of the indigenous Beauveria isolate. Moreover, it also exhibited the efficacy of the reference and commercially available biocontrol agents.

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Non rhizobial endophytic bacteria from chickpea (Cicer arietinum L.) tissues and their antagonistic traits

Journal of Applied and Natural Science ◽

10.31018/jans.v11i2.2056 ◽

2019 ◽

Vol 11 (2) ◽

pp. 346-351

Author(s):

Deepika Chhabra ◽

Poonam Sharma

Keyword(s):

Plant Growth ◽

Indole Acetic Acid ◽

Acc Deaminase ◽

Bacterial Isolates ◽

Cicer Arietinum L ◽

Fluorescent Pigment ◽

Biocontrol Activity ◽

Plant Growth Promoting ◽

Gibberelic Acid ◽

Growth Promoting

Bacteria that colonize plant tissues other than rhizobia and are beneficial for plant growth referred to non rhizobial plant growth-promoting endophytic bacteria (PGPEB). This study was designed to assay the biocontrol activity of plant growth promoting endophytic bacterial isolates those found positive for P. solubilization, ACC deaminase, Indole acetic acid and Gibberelic acid production. These bacterial isolates were obtained from chickpea (Cicer arietinum L.) tissues (roots and nodules). In a previous study a total of 263 non rhizobial endophytic bacterial isolates were isolated. Out of 263 isolates, 64.5% and 34.5% were Gram positive and negative, respectively. Further for biochemical characterization, catalase, oxidase, citrate utilization, nitrate reduction, methyl red and Voges Proskauer’s tests, were performed. On the basis of P solubilization, ACC deaminase, Indole acetic acid and Gibberelic acid production 75 potential isolates were selected and screened for their biocontrol activity viz. (production of cell wall degrading enzymes, production of HCN and fluorescent pigment). Out of 75 isolates, only 29 isolates produced cellulase, 64 isolates were able to produce protease and 28 were positive for both cellulose and protease. Of 75 endophytic isolates 12 isolates (7 from root tissue and 5 from nodules tissue, respectively) were positive for HCN production and 16 isolates were found to be fluorescent pigment producer under µv ligh. As chemical fertilizers and pesticides have detrimental effects on the environment. So these bacterial endophytic isolates will be used not only as a biofertilizer because of their plant growth promotional activities but also used as an alternative of synthetic chemicals for control of several plant diseases.

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The entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana promote systemic immunity and confer resistance to a broad range of pests and pathogens in tomato

Phytopathology ◽

10.1094/phyto-08-21-0343-r ◽

2021 ◽

Author(s):

Rupali Gupta ◽

Ravindran Keppanan ◽

Meirav Leibman-Markus ◽

Dalia Rav David ◽

Yigal Elad ◽

...

Keyword(s):

Disease Resistance ◽

Plant Growth ◽

Beauveria Bassiana ◽

Entomopathogenic Fungi ◽

Plant Pathogens ◽

Plant Immunity ◽

Systemic Immunity ◽

Metarhizium Brunneum ◽

Promote Plant Growth ◽

Arthropod Pests

Biocontrol agents can control pathogens by re-enforcing systemic plant resistance through systemic acquired resistance (SAR) or induced systemic resistance (ISR). Trichoderma spp. can activate the plant immune system through ISR, priming molecular mechanisms of defense against pathogens. Entomopathogenic fungi (EPF) can infect a wide range of arthropod pests, and play an important role in reducing pests' population. Here, we investigated the mechanisms by which EPF control plant diseases. We tested two well studied EPF, Metarhizium brunneum isolate Mb7 and Beauveria bassiana as the commercial product Velifer, for their ability to induce systemic immunity and disease resistance against several fungal and bacterial phytopathogens, and their ability to promote plant growth. We compared the activity of these EPF to an established biocontrol agent, T. harzianum T39, a known inducer of systemic plant immunity and broad disease resistance. The three fungal agents were effective against several fungal and bacterial plant pathogens and arthropod pests. Our results indicate that EPF induce systemic plant immunity and disease resistance by activating the plant host defense machinery, as evidenced by increases in reactive oxygen species (ROS) production and defense gene expression, and that EPF promote plant growth. EPF should be considered as control means for Tuta absoluta. We demonstrate that, with some exceptions, biocontrol in tomato can be equally potent by the tested EPF and T. harzianum T39, against both insect pests and plant pathogens. Taken together, our findings suggest that EPF may find use in broad-spectrum pest and disease management and as plant growth promoting agents.

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Effect of triapenthenol plant-growth regulator on canola height, yield, side branches and pod density

Canadian Journal of Plant Science ◽

10.4141/cjps92-140 ◽

1992 ◽

Vol 72 (4) ◽

pp. 1153-1156 ◽

Cited By ~ 2

Author(s):

K. J. Kirkland

Keyword(s):

Brassica Napus ◽

Plant Growth ◽

Field Experiment ◽

Plant Height ◽

Growth Regulator ◽

Plant Growth Regulator ◽

Growth And Development ◽

Management Tool ◽

Brassica Napus L ◽

Growing Conditions

A 3-yr field experiment was conducted at the Scott Experimental Farm to determine the effect of the growth regulator, triapenthenol, on the growth and development of Argentine canola (Brassica napus L.). Triapenthenol reduced plant height 25–45 cm under optimal growing conditions. Increases in yield, branches and total pods were observed. Application at the bud stage was more effective than earlier treatment in the rosette. It was concluded that triapenthenol application to canola could be a useful management tool, particularly under optimal growing conditions.Key words: Canola, growth regulator, RSW-0411, lodging

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Effect of Biological Organic Fertilizer on Plant Growth and Yield of Chinese Kale

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.142.175 ◽

2011 ◽

Vol 142 ◽

pp. 175-179 ◽

Cited By ~ 1

Author(s):

Shi Wei Song ◽

Hai Da Li ◽

Ri Yuan Chen ◽

Guang Wen Sun ◽

Hou Cheng Liu

Keyword(s):

Plant Growth ◽

Organic Fertilizer ◽

Growth And Yield ◽

Brassica Alboglabra ◽

Chinese Kale ◽

Medium Level ◽

Use Efficiency ◽

High Level ◽

High Fertilization ◽

Level Medium

A substrate culture experiment was conducted to study the effect of different biological organic fertilizer levels (control: zero fertilization, low level, medium level and high level) on plant growth and yield of Chinese kale (Brassica alboglabra Bailey). Results showed that compared with control, treatments of medium and high fertilization increased the leaf number, plant height, stem diameter, yield and plant dry matter of Chinese kale. Thus they promoted plant growth. The fertilizer utilization ratio of Chinese kale was the lowest for low fertilization treatment, and it was the highest for medium fertilization treatment, while it was decreased for high fertilization treatment. The treatment of medium level fertilization (30g/plant) promoted plant growth and increased the yield of Chinese kale; also it had the highest fertilizer use efficiency. So it was the appropriate organic fertilization amount for Chinese kale production.

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Soil, Plant, and Canopy Responses To Carbonated Irrigation Water

HortTechnology ◽

10.21273/horttech.6.2.111 ◽

1996 ◽

Vol 6 (2) ◽

pp. 111-114 ◽

Cited By ~ 2

Author(s):

Craig A. Storlie ◽

Joseph R. Heckman

Keyword(s):

Plant Growth ◽

Irrigation Water ◽

Growth Response ◽

Plant Productivity ◽

Field Studies ◽

Stimulate Plant Growth ◽

Carbonated Water ◽

Growing Conditions ◽

Increase Plant Productivity ◽

Increase Crop Yield

Scientists have sought to stimulate plant growth using carbonated irrigation water for more than 100 years. The mechanisms by which carbonated water may increase plant productivity and the influence of environmental and cultural growing conditions on those mechanisms are not completely understood. Several greenhouse and field studies have demonstrated that carbonated irrigation water can increase crop yield significantly while others have shown that carbonated irrigation water does not influence plant productivity. It is unlikely that carbonated irrigation water will be recommended commercially until the conditions are delineated under which a positive and economically advantageous growth response is ensured.

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