scholarly journals Identification of bioactive compounds in leaves and fruits of Actinidia arguta accessions from northeastern China and assessment of their antioxidant activity with a radical-scavenging effect

2021 ◽  
Vol 35 (1) ◽  
pp. 593-607
Author(s):  
Changhua Tan ◽  
Zhigang Wang ◽  
Xiuli Feng ◽  
Muhammad Irfan ◽  
Liu Changjiang
2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Mayank Gangwar ◽  
Manish Kumar Gautam ◽  
Amit Kumar Sharma ◽  
Yamini B. Tripathi ◽  
R. K. Goel ◽  
...  

Mallotus philippinensisis an important source of molecules with strong antioxidant activity widely used medicinal plant. Previous studies have highlighted their anticestodal, antibacterial, wound healing activities, and so forth. So, present investigation was designed to evaluate the total antioxidant activity and radical scavenging effect of 50% ethanol fruit glandular hair extract (MPE) and its role on Human Erythrocytes. MPE was tested for phytochemical test followed by its HPLC analysis. Standard antioxidant assays like DPPH, ABTS, hydroxyl, superoxide radical, nitric oxide, and lipid peroxidation assay were determined along with total phenolic and flavonoids content. Results showed that MPE contains the presence of various phytochemicals, with high total phenolic and flavonoid content. HPLC analysis showed the presence of rottlerin, a polyphenolic compound in a very rich quantity. MPE exhibits significant strong scavenging activity on DPPH and ABTS assay. Reducing power showed dose dependent increase in concentration absorption compared to standard, Quercetin. Superoxide, hydroxyl radical, lipid peroxidation, nitric oxide assay showed a comparable scavenging activity compared to its standard. Our finding further provides evidence thatMallotusfruit extract is a potential natural source of antioxidants which have a protective role on human Erythrocytes exhibiting minimum hemolytic activity and this justified its uses in folklore medicines.


2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Sri Adi Sumiwi ◽  
Anas Subarnas ◽  
Supriyatna Supriyatna ◽  
Marline Abdassah Bratadiredja

Sintoc (Cinnamomum sintoc Bl.) is a plant which is used as medicine. This plant has been known to have an analgesic antiinflamatory activity, therefore it is predicted to have an antioxidant activity. An investigation on antioxidant activity of sintoc essential oils and ethanolic extract of its cortex using ascorbic acid as standard has been carried out. Essential oils and ethanol extract of sintoc cortex was tested using DPPH (1,1-diphenyl-2-pikril-hidrazil) by measuring absorbance using visible spectrophotometer at 518 nm. The methods of this research were distillation of essential oils and extraction of sintoc cortex, determination of the essential oil and extract concentrations required for 50% inhibition of DPPH radical scavenging effect (IC50) with ascorbic acid as the possitive control. The variation concentration  of essential oils are 15, 5, 1, 0.1, 0.5 ppm and 25, 20, 17, 15, 10 ppm for ethanolic extracts. The results showed that the essential oil showed antioxidant activity with IC50 value was 16.29 ppm (5 times lower than ascorbic acid) and then ethanolic extract showed IC50 value 38.89 ppm (11 times lower than ascorbic acid, IC50 of ascorbic acid was 3.35 ppm).


2020 ◽  
Vol 11 (SPL3) ◽  
pp. 757-759
Author(s):  
Nithyanandham Masilamani ◽  
Dhanraj Ganapathy

Chlorogenic Acid (CA) is the formation of an ester established among caffeic and quinic acid. CA contains hydroxyl based groups over an aromatic moiety and exhibits very effective anticancer, antimutational and antioxidant activities. This survey was performed for assessing the awareness about antioxidant activities of CA amongst dental students. A cross-sectional survey was performed with a pretested questionnaire with ten questions disseminated among 100 dental students. The questionnaire assessed the awareness about CA therapy, their dietary sources, medicinal uses, antioxidant activity, anti ischaemic activity and free radical scavenging effect of chlorogenic acid. The responses were recorded and analyzed. 7% were aware of dietary sources,5% were aware of medical uses, 5% were aware of mechanisms of antioxidant activity, 4 % were aware of the anti ischaemic activity, and 5% were aware of the free radical scavenging effect of chlorogenic acid. Awareness about the usage of CA therapy is very less among dental students. Increased awareness programs and sensitization programs should be conducted to improve the awareness levels.


2013 ◽  
Vol 14 (1) ◽  
pp. 87-94 ◽  
Author(s):  
Gan B Bajracharya ◽  
Rokesh Maharjan

This study was aimed to evaluate the cytotoxicity of methanolic extract (ME) and aqueous extract (AE) of the rhizomes of Bergenia purpurascens through the Brine-shrimp bioassay, which revealed that ME possessed cytotoxicity with LC50 = 181.97 ìg/ml value. The plant extracts were also evaluated in vitro for antioxidant activity using different redox [Folin-Ciocalteu (FC) and Ferric Reducing Antioxidant Power (FRAP)] and radical scavenging [(2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)] assays. Both ME and AE possessed antioxidant activity and ME displayed comparatively a greater potential. The total phenolic contents in ME and AE was 1.27 and 0.06 g gallic acid equivalent (GAE)/100 g dry mass (DM), respectively. The FRAP values determined for ME and AE were 15.82 and 2.58 ìmol Fe(II)/100 g DM, respectively. The DPPH radical scavenging effect (IC50) was for ME 19.86 ìg/ml and 295.91 ìg/ml for AE. At 100 ìg/ml concentration, up to 94% inhibition of ABTS+ was observed in 6 min incubation. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 87-94 DOI: http://dx.doi.org/10.3126/njst.v14i1.8927


2002 ◽  
Vol 57 (3-4) ◽  
pp. 395-402 ◽  
Author(s):  
Ahmed G. Hegazi ◽  
Faten K. Abd El Hady

The free radical scavenging effect of two propolis samples collected from reclaimed land, Egypt as well as of vitamin C and caffeic acid in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical system was determined. The antimicrobial (Staphylococcus aureus; Escherichia coli and Candida albicans) activity was also investigated. The results of the free radical scavenging effect of El-Saff and Ismailia propolis showed a concentration-dependent activity. The antioxidant activity was varied according to the examined material. It was obvious that caffeic acid and vitamin C showed the highest activity if compared with the propolis samples. El- Saff propolis had a higher antioxidant activity than Ismailia propolis, it showed a higher antibacterial activity against Staphylococcus aureus and a higher anti-fungal activity against Candida albicans. While the Ismailia propolis had a higher antibacterial activity against Escherichia coli, than El-Saff propolis. The chemical composition of propolis samples was investigated by GC/MS, where 75 compounds were identified, 22 being new for propolis. The Ismailia propolis was characterized by the presence of a highly significant amount of aromatic acid esters (47.3%) and triterpenoids (17.3%), while El-Saff propolis contained 3% and 1.9% respectively. The new esters belonged to 4-methoxyhydrocinnamic acid, hydroferulic acid and ferulic acid. El-Saff propolis had a very high significant amount (27%) of 2,6-bis-(pentanyloxy)-4-pentanylphenethanol, which is also a new compound for propolis


2019 ◽  
Vol 15 (2) ◽  
pp. 156-164 ◽  
Author(s):  
Reza Farahmandfar ◽  
Maryam Asnaashari ◽  
Yegane Asadi ◽  
Batool Beyranvand

Background: It is important to study about the use of natural antioxidants as alternatives to synthetic ones due to the possibility of carcinogenic effects of synthetic antioxidants. This study is comparing the effect of the ultrasound-assisted and maceration extraction methods on antioxidant activity of Matricaria recutita. Methods: Bioactive compounds including phenolic, tocopherol, flavonoid and tannins and antioxidant activity of the extracts were evaluated. Moreover, extracts obtained from ultrasound and maceration methods were added to sunflower oil without any antioxidants at level of 200, 500 and 800 ppm, after that samples were heated at 180°C. Oxidation of the samples were evaluated after 0, 4, 8, 12, 16, 20 and 24 hours by measuring Peroxide Value (PV), Conjugated Diene (CD), Iodine Value (IV), Carbonyl Value (CV), Total Polar Compounds (TPC), Oil Stability Index (OSI), Color Index (CI) and acid value (AV). Results: The result showed total phenol (42.90 mg gallic acid/g extract), tocopherol (120.46 µg α - tocopherol/ml extract), flavonoid (2.64 mg/100 g extract) and tannins (3.89 mg gallic acid/g extract) of ultrasound extracts were higher than maceration extracts. Antioxidant activity of the extract was evaluated by DPPH assay which indicated 800 ppm of the Matricaria recutita extracted by ultrasound was the highest radical scavenging ability. Conclusion: Result indicated both ultrasound and maceration extracts could increase the oil oxidative stability but could not increase compared to BHA. In most cases, the extract samples by ultrasound had a better effect on stabilizing of sunflower oil during frying.


2012 ◽  
Vol 28 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Md Atiar Rahman ◽  
Rumana Sharmin ◽  
Md Nazim Uddin ◽  
Md Sohel Rana ◽  
Nazim Uddin Ahmed

Antibacterial effect of Crinum asiaticum bulb extract (1mg/disc) was tested on four Gram- positive and six Gram-negative bacteria by disc diffusion method using kanamycin (30 ìg/disc) as standard antibiotic disc. The bulb extract (250-1000mg/disc) showed significant zone of inhibition against all Gram-positive and Gram-negative bacteria ranging from 12-14 mm in diameter. Antioxidant potential of the same extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The extract showed remarkable free radical scavenging effect (95.96%) providing the IC50 value of 5.62 for the bulb extract and 5.46 for ascorbic acid (standard antioxidant) at the concentration of 1000 ìg/ml. The bulb extract was found to be (LC50 value 94.06 ?g/ml) in Brine-Shrimp lethality test. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11801 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 1-5


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