Comparison of UV C Light and Chemicals for Disinfection of Surfaces in Hospital Isolation Units

2006 ◽  
Vol 27 (7) ◽  
pp. 729-734 ◽  
Author(s):  
B. M. Andersen ◽  
H. Bånrud ◽  
E. Bøe ◽  
O. Bjordal ◽  
F. Drangsholt
Keyword(s):  
Bactericidal Effect ◽  
Patient Room ◽  
Environmental Cleaning ◽  
Before And After ◽  
Standard Contact ◽  
Uv C ◽  
Shadowed Area ◽  
Microbial Samples ◽  
Number Of Bacteria ◽  
Chemical Disinfection

Objective.To determine the bactericidal effect on surfaces of ceiling- and wall-mounted UV C (UVC) light (wavelength, 254 nm) in isolation units, compared with standard hospital environmental cleaning and chemical disinfection during final disinfection after patients are treated for infections.Design.Microbial samples were obtained from surfaces in isolation units (patient room, anteroom, and bathroom) before and after irradiation with UVC, chloramine disinfection, and standard hospital environmental cleaning. Samples were tested using standard contact plates.Setting.Four identical, negative air-pressure isolation units (patient room, anteroom, and bathroom) with a defined number of ceiling-and wall-mounted UVC light units. The UVC distribution was monitored in one isolation unit after irradiation for approximately 40 minutes, corresponding to doses ranging from 160 J/m2in a shadowed area to 19,230 J/m2at the mostly highly exposed site (which is high enough to inactivate most bacterial organisms, including spores).Results.UVC disinfection significantly reduced the number of bacteria on surfaces directly or indirectly exposed to UVC to a very low number, as did 5% chloramine disinfection alone (P<.001 for both). Completely shadowed areas in the isolation unit (eg, the bed rail, lockers, and mattresses) still required disinfection by chemicals.Conclusion.Disinfection with UVC light may significantly reduce environmental bacterial contamination and thereby protect the next patient housed in an isolation room. UVC disinfection may not be used alone but is a good addition to chemical disinfection.

scholarly journals Comparison of the effectiveness of manual cleaning, hydrogen peroxide vapor and ultraviolet-c in disinfection of hospital equipment

2020 ◽  
Vol 39 (3) ◽  
pp. 66-84 ◽  
Author(s):  
Yosra Sedaghat ◽  
Seifollah Gholampour ◽  
Farhad Tabatabai Ghomshe
Keyword(s):  
Hydrogen Peroxide ◽  
Complete Coverage ◽  
Hospital Beds ◽  
Before And After ◽  
Ultraviolet C ◽  
Pressure Meter ◽  
Uv C ◽  
Hospital Equipment ◽  
Hydrogen Peroxide Vapor ◽  
Number Of Bacteria

Hospitals are the focus of many infections which cause the intensification of the patients’ illness and even their death. The number of bacteria on various hospital equipment was counted before and after being disinfected with manual cleaning (MC), hydrogen peroxide vapour (HPV) and ultraviolet-C (UV-C). The effectiveness of disinfection of hospital beds in intensive care unit (ICU) using the three methods MC, HPV and UV-C has been 66.67%, 100% and 50%, respectively. Hence, the effectiveness of HPV method in the disinfection of this unit has been higher than others. The effectiveness of otoscope disinfection in the ICU and blood pressure meter in the operating room with three methods of MC, HPV and UV-C has been 0%, 50% and 100%, respectively. So, for disinfecting these small outfits, UV-C method has proven to be the best one. In case of programming, the correct move of portable UV-Cs for complete coverage of massive equipment, disinfection with UV-C method may be considered more effective than other methods.

APPLICATION OF THE ATP-BIOLUMINESCENCE METHOD TO ASSESS THE QUALITY OF SANITATION OF MILKING EQUIPMENT

2020 ◽  
Vol 1 (4) ◽  
pp. 468-472
Author(s):  
P.A. Popov ◽  
◽  
D.V. Gruznov ◽  
S.V. Tokarev ◽  
◽  
...  
Keyword(s):  
Dairy Farms ◽  
Laboratory Studies ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Microbial Number ◽  
Proportional Relationship ◽  
The Relationship ◽  
Number Of Bacteria ◽  
Bioluminescence Method

The article presents the results of determining the relationship between the total microbial number and microbial ATP on the surface of milking equipment before and after treatment with neutral anolyte ANK-SUPER. The possibility of using the ATP-bioluminescence method to control the quality of sanitation of milking equipment on dairy farms is shown. Laboratory studies revealed a proportional relationship between the level of bacterial ATP and the number of bacteria on the surface of milking equipment before and after sanitation.

ON THE APPLICATION OF DISPERSION ANALYSIS FOR QUALITY ASSESSMENT OF A QUARTZ UV IRRADIATOR

2021 ◽  
pp. 52-57
Author(s):  
D. D. Balandin ◽  
O. L. Lyubimtseva
Keyword(s):  
Analysis Of Variance ◽  
Software Package ◽  
Bactericidal Effect ◽  
Dispersion Analysis ◽  
Anova Model ◽  
Urgent Task ◽  
Uv Lamp ◽  
Quality Of Products ◽  
Uv C

Ensuring and improving the quality of products is currently an urgent task implemented through product quality management. In connection with the spread of the COVID-19 virus, Rospotrebnadzor recommends the use of quartz lamps when disinfecting premises. The article discusses the issue of assessing the quality of a device for home use of a quartz ultraviolet irradiator OUFK-01. In accordance with the WHO recommendations, the irradiation indicator became the main one for the study. The study was carried out using an ANOVA model with several iterations. Purpose of the study: to study the variability of the irradiance index in different UV ranges depending on the source of UV radiation (UV lamp) of the ultraviolet irradiator OUFK-01. The main advantage of the analysis of variance was used – the ability to consider the interaction of various factors. The conclusions that were formulated based on the results of analysis of variance suggest that the quality of the OUFK-01 irradiator has changed. Data processing was carried out using the MS EXCEL software package. The article provides a rationale for what is meant by “quality improvement”, namely, in order not to violate the bactericidal effect, it is necessary to reduce the UV-A and UV-B ranges to acceptable values, since the effect of UV-C rays has not been fully studied, then the irradiance should be increased to the permissible wavelength range. A comparative analysis of successively modified products showed that the quality of the device increased with the replacement of the UV lamp.

scholarly journals Contamination of SARS-CoV-2 in patient surroundings and on personal protective equipment in a non-ICU isolation ward for COVID-19 patients with prolonged PCR positive status

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Li Wei ◽  
Wenzhi Huang ◽  
Xiaojun Lu ◽  
Yantong Wang ◽  
Linzhi Cheng ◽  
...  
Keyword(s):  
Healthcare Workers ◽  
Clinical Samples ◽  
Protective Equipment ◽  
Environmental Sampling ◽  
Personal Protection ◽  
Personal Protection Equipment ◽  
Environmental Cleaning ◽  
Air Samples ◽  
Before And After ◽  
Isolation Ward

Abstract Objectives We performed an environmental sampling study to investigate the environmental contamination of SARS-CoV-2 by COVID-19 patients with prolonged PCR positive status of clinical samples. Methods We sampled the air from rooms for nine COVID-19 patients with illness or positive PCR > 30 days, before and after nasopharyngeal/oropharyngeal swabbing and before and after nebulization treatment. We also sampled patients’ surroundings and healthcare workers’ personal protection equipment (PPE) in a non-ICU ward. SARS-CoV-2 was detected by PCR. Results Eighty-eight samples were collected from high-touch surfaces and floors in patient rooms and toilets, with only the bedsheets of two patients and one toilet positive for SARS-CoV-2. All air samples (n = 34) were negative for SARS-CoV-2. Fifty-five samples collected from PPE were all negative. Conclusion Contamination of near-patient surroundings was uncommon for COVID-19 patients with prolonged PCR positive status if environmental cleaning/disinfection were performed rigorously. Airborne transmission of SARS-CoV-2 was unlikely in these non-ICU settings.

scholarly journals Decontamination of Targeted Pathogens from Patient Rooms Using an Automated Ultraviolet-C-Emitting Device

2013 ◽  
Vol 34 (5) ◽  
pp. 466-471 ◽  
Author(s):  
Deverick J. Anderson ◽  
Maria F. Gergen ◽  
Emily Smathers ◽  
Daniel J. Sexton ◽  
Luke F. Chen ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Environmental Services ◽  
Convenience Sample ◽  
Vancomycin Resistant Enterococci ◽  
Colony Forming Units ◽  
Before And After ◽  
Ultraviolet C ◽  
Vancomycin Resistant ◽  
Tertiary Care Hospitals ◽  
Uv C

Objective.To determine the effectiveness of an automated ultraviolet-C (UV-C) emitter against vancomycin-resistant enterococci (VRE),Clostridium difficile, andAcinetobacterspp. in patient rooms.Design.Prospective cohort study.Setting.Two tertiary care hospitals.Participants.Convenience sample of 39 patient rooms from which a patient infected or colonized with 1 of the 3 targeted pathogens had been discharged.Intervention.Environmental sites were cultured before and after use of an automated UV-C-emitting device in targeted rooms but before standard terminal room disinfection by environmental services.Results.In total, 142 samples were obtained from 27 rooms of patients who were colonized or infected with VRE, 77 samples were obtained from 10 rooms of patients withC. difficileinfection, and 10 samples were obtained from 2 rooms of patients with infections due toAcinetobacter. Use of an automated UV-C-emitting device led to a significant decrease in the total number of colony-forming units (CFUs) of any type of organism (1.07 log10reduction;P< .0001), CFUs of target pathogens (1.35 log10reduction;P< .0001), VRE CFUs (1.68 log10reduction;P< .0001), and C.difficileCFUs (1.16 log10reduction;P< .0001). CFUs ofAcinetobacteralso decreased (1.71 log10reduction), but the trend was not statistically significantP= .25). CFUs were reduced at all 9 of the environmental sites tested. Reductions similarly occurred in direct and indirect line of sight.Conclusions.Our data confirm that automated UV-C-emitting devices can decrease the bioburden of important pathogens in real-world settings such as hospital rooms.

Efficacy of Germicidal Hand Wash Agents in Hygienic Hand Disinfection1

1982 ◽  
Vol 45 (8) ◽  
pp. 713-720 ◽  
Author(s):  
A. Z. SHEENA ◽  
M. E. STILES
Keyword(s):  
Staphylococcus Epidermidis ◽  
Latin Square ◽  
Sampling Technique ◽  
Ammonium Compound ◽  
Food Handlers ◽  
Hand Wash ◽  
Before And After ◽  
Finger Tip ◽  
Number Of Bacteria ◽  
Better Than

The efficacy of hygienic hand wash procedures for food handlers using germicidal soaps and hand dips was studied by measuring changes in numbers of microorganisms released from hands before and after each of two successive 15-s treatments. Both hand rinse and finger tip imprint sampling techniques were used. The experiment consisted of two (6 × 6) Latin square designs, each including a non-germicidal soap control. Of the hand dip agents, including sodium hypochlorite (50 ppm available chlorine), iodophor (25 ppm available iodine) and a quaternary ammonium compound (QAC) (930 ppm benzalkonium chloride), only the QAC gave a statistically significant decrease in the number of bacteria released when tested by the finger imprint technique. This experiment included a bar soap containing 1.0% trichlorocarbanilide which gave results equivalent to the non-germicidal soap control. Of the hand wash agents, 4% chlorhexidine gluconate and iodophor (0.75% available iodine) resulted in significant decreases in numbers of bacteria released when tested by either sampling technique. Products containing Irgasan DP 300 (0.25% active ingredient at the use concentration), tribromosalicylanilide (0.5%) and para-chloro-meta-xylenol (0.325%) were no better than the non-germicidal soap control under the conditions of this experiment. Identification of 3,591 aerobic isolates from finger imprint plates indicated that Staphylococcus epidermidis and Micrococcus spp. were the predominating organisms (85.3%) released from the hands.

scholarly journals New Local Drug Delivery with Antibiotic in the Nonsurgical Treatment of Periodontitis—Pilot Study

2019 ◽  
Vol 9 (23) ◽  
pp. 5077
Author(s):  
Aleksandra Sender-Janeczek ◽  
Jacek Zborowski ◽  
Małgorzata Szulc ◽  
Tomasz Konopka
Keyword(s):  
Nonsurgical Treatment ◽  
Local Drug Delivery ◽  
Dental Arch ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Promising Alternative ◽  
Before And After ◽  
Preliminary Study ◽  
Number Of Bacteria

Combination of the classical subgingival instrumentation (scaling and root planing procedure, SRP) with an antibiotic administered to periodontal pockets in a suitable medium is a promising alternative protocol of nonsurgical periodontal treatment. It enables obtaining the long-term minimum drug concentration inhibiting the development of periopathogens. Objectives: Clinical and microbiological evaluation of periodontal pockets two months after single application of a gel containing piperacillin and tazobactam (Gelcide)® in relation to the nonsurgical treatment procedure (SRP). Materials and methods: Ten patients aged 24–56 years (mean 39.5) with chronic periodontitis, nonsmokers with acceptable oral hygiene and no classical exclusion criteria were qualified for treatment. In the maxilla area, SRP was performed and the assessed gel was inserted to two randomly selected adjacent periodontal pockets. Clinical evaluation included the assessment of bleeding on probing (BoP), pocket depth (PD), and clinical attachment loss (CAL) at six measurement points. A microbiological examination with the use of PET deluxe diagnostic kit in the drug-administered pockets and symmetrically in two pockets on the other side of the dental arch was performed. The examination was conducted before the treatment and two months later. Results: Two months after the treatment, a significant improvement in all analyzed clinical parameters was observed. However, the extent of this improvement did not differ significantly between the compared treatment methods. No statistically significant differences were found in the number of bacteria before and after the treatment, except for a significant decrease in the number of Micromonas micros (2957 vs. 589, p = 0.028) and a higher number of the green complex bacteria Capnocytophaga gingivalis (5439 vs. 2050, p = 0.041) after antibiotic had been used. Conclusion: No significant clinical and microbiological differences were found after additional administration of gel with piperacillin and tazobactam in relation to SRP in the preliminary study.

scholarly journals Hygienisation of Surplus Activated Sludge by Hydrodynamic Cavitation

2012 ◽  
Vol 19 (3) ◽  
pp. 415-421
Author(s):  
Alicja Machnicka ◽  
Klaudiusz Grűbel ◽  
Agnieszka Rusin
Keyword(s):  
Activated Sludge ◽  
Pathogenic Bacteria ◽  
Bacterial Culture ◽  
Hydrodynamic Cavitation ◽  
Chemical Methods ◽  
The Family ◽  
Before And After ◽  
Microbiological Analyses ◽  
Number Of Bacteria ◽  
Surrounding Liquid

Hygienisation of Surplus Activated Sludge by Hydrodynamic CavitationThe success of an effective process of disintegration is the application of physical or chemical methods of the destruction of the cell wall which consequently becomes fragmented and the intracellular matters are released into the surrounding liquid. The purpose of the conducted experiments was to demonstrate possibilities of application of the hydrodynamic in the bacteriological hygienisation of surplus activated sludge. The confirmation of the effectiveness of the bactericidal activity of hydrodynamic cavitation was the number of bacteria grown on agars before and after hygienisation process. Selective and basic beddings have been used in the research. The bacterial culture has lasted approximately 24 to 48 hours. After the period of incubation the colonies produced have been counted and initially identified. Based on the microbiological analyses a significant reduction in an overall number of bacteria and pathogenic bacteria belonging to the familyEnterobacteriaceaeand to the pathogenic speciesStaphylococcushas been noticed. The overall number of bacteria decrease about 80% after 45 minutes of disintegration of surplus activated sludge. Pathogenic bacteria belonging to the familyEnterobacteriaceae: lactose-positive and lactose-negative decrease about 93% and 92%, respectively. Reducing the number ofSalmonellabacteria was 100% after 45 minutes of disintegration processes. Reduction bacteria of speciesStaphylococcuswere about 93% for mannitolo-positive and 92% for mannitolo-negative.

scholarly journals Effect of a UV-C Automatic Last-Generation Mobile Robotic System on Multi-Drug Resistant Pathogens

2021 ◽  
Vol 18 (24) ◽  
pp. 13019
Author(s):  
Carla Russo ◽  
Desirée Bartolini ◽  
Cristina Corbucci ◽  
Anna Maria Stabile ◽  
Mario Rende ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Irradiation Effect ◽  
Healthcare Associated Infections ◽  
Drug Resistant ◽  
Mortality And Morbidity ◽  
Disinfection Methods ◽  
Healthcare Associated ◽  
Uv C ◽  
Number Of Bacteria

Background: Healthcare-associated infections caused by multi-drug resistant (MDR) pathogens are associated with increased mortality and morbidity among hospitalized patients. Inanimate surfaces, and in particular high-touch surfaces, have often been described as the source for outbreaks of nosocomial infections. The present work aimed to evaluate the efficacy of a last-generation mobile (robotic) irradiation UV-C light device R2S on MDR microorganisms in inanimate surfaces and its translation to hospital disinfection. Methods: The efficacy of R2S system was evaluated in environmental high-touch surfaces of two separate outpatient rooms of Perugia Hospital in Italy. The static UV-C irradiation effect was investigated on both the bacterial growth of S. aureus, MRSA, P. aeruginosa, and K. pneumoniae KPC and photoreactivation. The antimicrobial activity was also tested on different surfaces, including glass, steel, and plastic. Results: In the environmental tests, the R2S system decreased the number of bacteria, molds, and yeasts of each high-touch spot surface (HTSs) compared with manual sanitization. UV-C light irradiation significantly inhibits in vitro bacterial growth, also preventing photoreactivation. UV-C light bactericidal activity on MDR microorganisms is affected by the type of materials of inanimate surfaces. Conclusions: The last-generation mobile R2S system is a more reliable sanitizing procedure compared with its manual counterpart.

scholarly journals Investigations of influence of disinfection procedures on hygiene in private slaughterhouse

2012 ◽  
Vol 66 (3-4) ◽  
pp. 219-231
Author(s):  
Ljiljana Jankovic ◽  
Brana Radenkovic-Damnjanovic ◽  
Nedjeljko Karabasil ◽  
Milorad Mirilovic ◽  
Slobodan Maric
Keyword(s):  
Standard Method ◽  
Exposure Period ◽  
Colony Count ◽  
Sampling Techniques ◽  
Commission Decision ◽  
Skilled Professional ◽  
Before And After ◽  
Sodium Dichloroisocyanurate ◽  
Animal Feeding ◽  
Number Of Bacteria

The objective of this work was to establish, on the grounds of obtained results for the total number of bacteria before and after completed disinfection, whether there are differences in the efficiency of disinfection performed by a professional and disinfection carried out by an unqualified employee in a private slaughterhouse. The material used in these investigations were samples of wet-dry swabs taken over a course of five weeks, before and after disinfection carried out by an unqualified employee and the skilled professional, from the following: the knife used for evisceration, the floor in the evisceration area, from the table serving for bristle removal, and from the floor underneath the bristle removal surface. The wet-dry swabs were taken according to the procedure described in the standard method ISO 18593 (Microbiology of food and animal feeding stuffs - Horizontal methods for sampling techniques from surfaces using contact plates and swabs). Analyzing the taken samples, the total number of bacteria was determined using the standard method ISO 4833 (Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30?C). Disinfection was carried out using a chlorine preparation (sodium dichloroisocyanurate dihydrate) in a concentration of 0.02% and for an exposure period of 30 min. The results were interpreted on the grounds of the border values in evaluating the hygiene of the equipment, tools, and work surfaces, presented in Commission Decision 471/2001/ EC. The results of the investogations have shown that the disinfection performed by the skilled professional was more efficient than the disinfection performed by the unqualified person, as the total number of bacteria was significantly smaller (p<0.01) in the course of all 5 experimental weeks on the evisceration knife, the floor under the bristle removal surface, during weeks 1, 2 and 5, and on the floor in the evisceration area in weeks 1 and 5.

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