scholarly journals Comparison of the effectiveness of manual cleaning, hydrogen peroxide vapor and ultraviolet-c in disinfection of hospital equipment

2020 ◽  
Vol 39 (3) ◽  
pp. 66-84 ◽  
Author(s):  
Yosra Sedaghat ◽  
Seifollah Gholampour ◽  
Farhad Tabatabai Ghomshe
Keyword(s):  
Hydrogen Peroxide ◽  
Complete Coverage ◽  
Hospital Beds ◽  
Before And After ◽  
Ultraviolet C ◽  
Pressure Meter ◽  
Uv C ◽  
Hospital Equipment ◽  
Hydrogen Peroxide Vapor ◽  
Number Of Bacteria

Hospitals are the focus of many infections which cause the intensification of the patients’ illness and even their death. The number of bacteria on various hospital equipment was counted before and after being disinfected with manual cleaning (MC), hydrogen peroxide vapour (HPV) and ultraviolet-C (UV-C). The effectiveness of disinfection of hospital beds in intensive care unit (ICU) using the three methods MC, HPV and UV-C has been 66.67%, 100% and 50%, respectively. Hence, the effectiveness of HPV method in the disinfection of this unit has been higher than others. The effectiveness of otoscope disinfection in the ICU and blood pressure meter in the operating room with three methods of MC, HPV and UV-C has been 0%, 50% and 100%, respectively. So, for disinfecting these small outfits, UV-C method has proven to be the best one. In case of programming, the correct move of portable UV-Cs for complete coverage of massive equipment, disinfection with UV-C method may be considered more effective than other methods.

scholarly journals Decontamination of Targeted Pathogens from Patient Rooms Using an Automated Ultraviolet-C-Emitting Device

2013 ◽  
Vol 34 (5) ◽  
pp. 466-471 ◽  
Author(s):  
Deverick J. Anderson ◽  
Maria F. Gergen ◽  
Emily Smathers ◽  
Daniel J. Sexton ◽  
Luke F. Chen ◽  
...  
Keyword(s):  
Tertiary Care ◽  
Environmental Services ◽  
Convenience Sample ◽  
Vancomycin Resistant Enterococci ◽  
Colony Forming Units ◽  
Before And After ◽  
Ultraviolet C ◽  
Vancomycin Resistant ◽  
Tertiary Care Hospitals ◽  
Uv C

Objective.To determine the effectiveness of an automated ultraviolet-C (UV-C) emitter against vancomycin-resistant enterococci (VRE),Clostridium difficile, andAcinetobacterspp. in patient rooms.Design.Prospective cohort study.Setting.Two tertiary care hospitals.Participants.Convenience sample of 39 patient rooms from which a patient infected or colonized with 1 of the 3 targeted pathogens had been discharged.Intervention.Environmental sites were cultured before and after use of an automated UV-C-emitting device in targeted rooms but before standard terminal room disinfection by environmental services.Results.In total, 142 samples were obtained from 27 rooms of patients who were colonized or infected with VRE, 77 samples were obtained from 10 rooms of patients withC. difficileinfection, and 10 samples were obtained from 2 rooms of patients with infections due toAcinetobacter. Use of an automated UV-C-emitting device led to a significant decrease in the total number of colony-forming units (CFUs) of any type of organism (1.07 log10reduction;P< .0001), CFUs of target pathogens (1.35 log10reduction;P< .0001), VRE CFUs (1.68 log10reduction;P< .0001), and C.difficileCFUs (1.16 log10reduction;P< .0001). CFUs ofAcinetobacteralso decreased (1.71 log10reduction), but the trend was not statistically significantP= .25). CFUs were reduced at all 9 of the environmental sites tested. Reductions similarly occurred in direct and indirect line of sight.Conclusions.Our data confirm that automated UV-C-emitting devices can decrease the bioburden of important pathogens in real-world settings such as hospital rooms.

scholarly journals Evaluation of a Pulsed Xenon Ultraviolet Disinfection System for Reduction of Healthcare-Associated Pathogens in Hospital Rooms

2015 ◽  
Vol 36 (2) ◽  
pp. 192-197 ◽  
Author(s):  
Michelle M. Nerandzic ◽  
Priyaleela Thota ◽  
Thriveen Sankar C. ◽  
Annette Jencson ◽  
Jennifer L. Cadnum ◽  
...  
Keyword(s):  
Uv Irradiation ◽  
Organic Load ◽  
Short Exposure ◽  
Glass Slides ◽  
Before And After ◽  
Ultraviolet C ◽  
Pathogen Recovery ◽  
Healthcare Associated ◽  
The Impact ◽  
Uv C

OBJECTIVETo determine the effectiveness of a pulsed xenon ultraviolet (PX-UV) disinfection device for reduction in recovery of healthcare-associated pathogens.SETTINGTwo acute-care hospitals.METHODSWe examined the effectiveness of PX-UV for killing ofClostridium difficilespores, methicillin-resistantStaphylococcus aureus(MRSA), and vancomycin-resistantEnterococcus(VRE) on glass carriers and evaluated the impact of pathogen concentration, distance from the device, organic load, and shading from the direct field of radiation on killing efficacy. We compared the effectiveness of PX-UV and ultraviolet-C (UV-C) irradiation, each delivered for 10 minutes at 4 feet. In hospital rooms, the frequency of native pathogen contamination on high-touch surfaces was assessed before and after 10 minutes of PX-UV irradiation.RESULTSOn carriers, irradiation delivered for 10 minutes at 4 feet from the PX-UV device reduced recovery ofC. difficilespores, MRSA, and VRE by 0.55±0.34, 1.85±0.49, and 0.6±0.25 log10colony-forming units (CFU)/cm2, respectively. Increasing distance from the PX-UV device dramatically reduced killing efficacy, whereas pathogen concentration, organic load, and shading did not. Continuous UV-C achieved significantly greater log10CFU reductions than PX-UV irradiation on glass carriers. On frequently touched surfaces, PX-UV significantly reduced the frequency of positiveC. difficile, VRE, and MRSA culture results.CONCLUSIONSThe PX-UV device reduced recovery of MRSA,C. difficile, and VRE on glass carriers and on frequently touched surfaces in hospital rooms with a 10-minute UV exposure time. PX-UV was not more effective than continuous UV-C in reducing pathogen recovery on glass slides, suggesting that both forms of UV have some effectiveness at relatively short exposure times.Infect Control Hosp Epidemiol 2014;00(0): 1–6

Comparison of the Microbiological Efficacy of Hydrogen Peroxide Vapor and Ultraviolet Light Processes for Room Decontamination

2012 ◽  
Vol 33 (5) ◽  
pp. 507-512 ◽  
Author(s):  
Nancy L. Havill ◽  
Brent A. Moore ◽  
John M. Boyce
Keyword(s):  
Hydrogen Peroxide ◽  
Prospective Observational Study ◽  
Biological Indicators ◽  
Line Of Sight ◽  
Geobacillus Stearothermophilus ◽  
Log Reduction ◽  
Before And After ◽  
The Mean ◽  
Mean Cycle ◽  
Hydrogen Peroxide Vapor

Objective.To compare the microbiological efficacy of hydrogen peroxide vapor (HPV) and ultraviolet radiation (UVC) for room decontamination.Design.Prospective observational study.Setting.500-bed teaching hospital.Methods.HPV and UVC processes were performed in 15 patient rooms. Five high-touch sites were sampled before and after the processes and aerobic colony counts (ACCs) were determined. Carrier disks with ~106Clostridium difficile (CD) spores and biological indicators (BIs) with 104 and 106Geobacillus stearothermophilus spores were placed in 5 sites before decontamination. After decontamination, CD log reductions were determined and BIs were recorded as growth or no growth.Results.93% of ACC samples that had growth before HPV did not have growth after HPV, whereas 52% of sites that had growth before UVC did not have growth after UVC (P < .0001). The mean CD log reduction was >6 for HPV and ~2 for UVC. After HPV 100% of the 104 BIs did not grow, and 22% did not grow after UVC, with a range of 7%–53% for the 5 sites. For the 106 BIs, 99% did not grow after HPV and 0% did not grow after UVC. Sites out of direct line of sight were significantly more likely to show growth after UVC than after HPV. Mean cycle time was 153 (range, 140–177) min for HPV and 73 (range, 39–100) min for UVC (P < .0001).Conclusion.Both HPV and UVC reduce bacterial contamination, including spores, in patient rooms, but HPV is significantly more effective. UVC is significantly less effective for sites that are out of direct line of sight.

Comparison of UV C Light and Chemicals for Disinfection of Surfaces in Hospital Isolation Units

2006 ◽  
Vol 27 (7) ◽  
pp. 729-734 ◽  
Author(s):  
B. M. Andersen ◽  
H. Bånrud ◽  
E. Bøe ◽  
O. Bjordal ◽  
F. Drangsholt
Keyword(s):  
Bactericidal Effect ◽  
Patient Room ◽  
Environmental Cleaning ◽  
Before And After ◽  
Standard Contact ◽  
Uv C ◽  
Shadowed Area ◽  
Microbial Samples ◽  
Number Of Bacteria ◽  
Chemical Disinfection

Objective.To determine the bactericidal effect on surfaces of ceiling- and wall-mounted UV C (UVC) light (wavelength, 254 nm) in isolation units, compared with standard hospital environmental cleaning and chemical disinfection during final disinfection after patients are treated for infections.Design.Microbial samples were obtained from surfaces in isolation units (patient room, anteroom, and bathroom) before and after irradiation with UVC, chloramine disinfection, and standard hospital environmental cleaning. Samples were tested using standard contact plates.Setting.Four identical, negative air-pressure isolation units (patient room, anteroom, and bathroom) with a defined number of ceiling-and wall-mounted UVC light units. The UVC distribution was monitored in one isolation unit after irradiation for approximately 40 minutes, corresponding to doses ranging from 160 J/m2in a shadowed area to 19,230 J/m2at the mostly highly exposed site (which is high enough to inactivate most bacterial organisms, including spores).Results.UVC disinfection significantly reduced the number of bacteria on surfaces directly or indirectly exposed to UVC to a very low number, as did 5% chloramine disinfection alone (P<.001 for both). Completely shadowed areas in the isolation unit (eg, the bed rail, lockers, and mattresses) still required disinfection by chemicals.Conclusion.Disinfection with UVC light may significantly reduce environmental bacterial contamination and thereby protect the next patient housed in an isolation room. UVC disinfection may not be used alone but is a good addition to chemical disinfection.

scholarly journals Approach of using a household device in decontaminating respirators with  ultraviolet C during the scarcity in the COVID-19 pandemic

2020 ◽  
Author(s):  
Darunee Chotiprasitsakul ◽  
Taya Kitiyakara ◽  
Anan Jongkaewwattana ◽  
Pitak Santanirand ◽  
Atisak Jiaranaikulwanich ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bacterial Culture ◽  
Polymer Fibers ◽  
Baby Bottle ◽  
Before And After ◽  
Ultraviolet C ◽  
N95 Respirators ◽  
Uv C ◽  
Scanning Electron

Abstract Background Reusing N95 respirators with a practical and easy decontamination protocol might be a solution for the shortage of N95 respirators. We aimed to study the reliability and safety of household UV-C devices for the decontamination of N95 respirators. Methods We investigated a baby bottle ultraviolet C (UV-C) sterilizer box for N95 decontamination. Swine coronavirus (PEDV) was sprayed on the N95 and the virus was cultivated after UV-C sterilization. Bacterial culture from used N95 respirator was performed before and after UV-C exposure. Scanning electron Microscopy (SEM) was used to observe the structural change after UV-C exposure. The radiation was measured by radiometer. The fit test was performed on 3 participants before use, and after reuse 4 and 6 times. Results The PEDV sprayed on the N95 respirator was inactivated by the UV-C level of 0.930–0.932 mW/cm2. Nearly all bacterial colonies had disappeared after the 10 min of the UV-C exposure. No significant change to the structure of the N95 polymer fibers after 240 min of UV-C exposure was observed by SEM. Three and two participants passed the fit test after the fourth and sixth time of reuse, respectively. Conclusions During the COVID-19 pandemic crisis, UV-C products may be practical and safe options for decontaminating N95 respirators, if the energy of UV-C is in the range known to deactivate the virus.

APPLICATION OF THE ATP-BIOLUMINESCENCE METHOD TO ASSESS THE QUALITY OF SANITATION OF MILKING EQUIPMENT

2020 ◽  
Vol 1 (4) ◽  
pp. 468-472
Author(s):  
P.A. Popov ◽  
◽  
D.V. Gruznov ◽  
S.V. Tokarev ◽  
◽  
...  
Keyword(s):  
Dairy Farms ◽  
Laboratory Studies ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Microbial Number ◽  
Proportional Relationship ◽  
The Relationship ◽  
Number Of Bacteria ◽  
Bioluminescence Method

The article presents the results of determining the relationship between the total microbial number and microbial ATP on the surface of milking equipment before and after treatment with neutral anolyte ANK-SUPER. The possibility of using the ATP-bioluminescence method to control the quality of sanitation of milking equipment on dairy farms is shown. Laboratory studies revealed a proportional relationship between the level of bacterial ATP and the number of bacteria on the surface of milking equipment before and after sanitation.

Antimicrobial Effect of 940 nm Diode Laser based on Photolysis of Hydrogen Peroxide in the Treatment of Periodontal Disease

2018 ◽  
Vol 69 (8) ◽  
pp. 2081-2088 ◽  
Author(s):  
Alin Alexandru Odor ◽  
Edwin Sever Bechir ◽  
Deborah Violant ◽  
Victoria Badea
Keyword(s):  
Hydrogen Peroxide ◽  
Laser Therapy ◽  
Diode Laser ◽  
Antimicrobial Effect ◽  
The Other ◽  
Control Group ◽  
Group 4 ◽  
Periodontal Bacteria ◽  
Severe Periodontitis ◽  
Before And After

Moderate and severe periodontitis represents a challenge in the non-surgical periodontal therapy. Due to the lack of evidence regarding the antimicrobial effectiveness of 940 nm diode laser in periodontal treatment, this study aimed to evaluate the antimicrobial effect of hydrogen peroxide (H2O2) photolysis performed with 940 nm diode laser in the treatment of moderate and severe periodontitis. Twenty-five patients with 100 teeth were selected for this pilot study. The test teeth were randomly assigned to one of the four treatment groups: Group 1: scaling and root planning (SRP) (control group); and the following experimental groups: Group 2: H2O2; Group 3: 940 nm diode laser therapy; Group 4: 940 nm diode laser therapy and H2O2. Clinical examinations, like probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were performed before and after the treatment. The microbiological evaluation, effectuated before and after the treatment, included nine periodontal bacteria species and investigated by means of real-time PCR assay. The clinical and bacterial differences in the tested groups, was assessed between control group and the other three experimental groups, as well as between the experimental groups. The total bacteria load was reduced for all four studied groups. Group 4 (diode laser + H2O2) showed significant bacterial reduction of the major periodontal bacteria like Pg., Tf., Td., Pi., Pm., Fn (p[0.001) than the other 3 groups (p]0.001). Also the periodontal clinical parameters, like PD, CAL and BOP showed a significant reduction after the photolysis of H2O2 with the 940 nm diode laser (p[0.001). Differences between tested groups showed a significant beneficial results in regard to Group 4.It is suggested that the photoactivation of H2O2 with the 940 nm diode laser can be used successfully in adjunctive to the non-surgical periodontal treatment as a bactericidal tool.

scholarly journals Effectiveness of Ultraviolet-C Room Disinfection on Preventing Healthcare-Associated Clostridioides difficile Infection

2020 ◽  
Vol 41 (S1) ◽  
pp. s33-s33
Author(s):  
Michihiko Goto ◽  
Erin Balkenende ◽  
Gosia Clore ◽  
Rajeshwari Nair ◽  
Loretta Simbartl ◽  
...  
Keyword(s):  
Acute Care ◽  
Acute Care Hospitals ◽  
Incidence Rates ◽  
Positive Test ◽  
Negative Binomial Regression Model ◽  
Test Results ◽  
Clostridioides Difficile ◽  
Ultraviolet C ◽  
Healthcare Associated ◽  
Uv C

Background: Enhanced terminal room cleaning with ultraviolet C (UVC) disinfection has become more commonly used as a strategy to reduce the transmission of important nosocomial pathogens, including Clostridioides difficile, but the real-world effectiveness remains unclear. Objectives: We aimed to assess the association of UVC disinfection during terminal cleaning with the incidence of healthcare-associated C. difficile infection and positive test results for C. difficile within the nationwide Veterans Health Administration (VHA) System. Methods: Using a nationwide survey of VHA system acute-care hospitals, information on UV-C system utilization and date of implementation was obtained. Hospital-level incidence rates of clinically confirmed hospital-onset C. difficile infection (HO-CDI) and positive test results with recent healthcare exposures (both hospital-onset [HO-LabID] and community-onset healthcare-associated [CO-HA-LabID]) at acute-care units between January 2010 and December 2018 were obtained through routine surveillance with bed days of care (BDOC) as the denominator. We analyzed the association of UVC disinfection with incidence rates of HO-CDI, HO-Lab-ID, and CO-HA-LabID using a nonrandomized, stepped-wedge design, using negative binomial regression model with hospital-specific random intercept, the presence or absence of UVC disinfection use for each month, with baseline trend and seasonality as explanatory variables. Results: Among 143 VHA acute-care hospitals, 129 hospitals (90.2%) responded to the survey and were included in the analysis. UVC use was reported from 42 hospitals with various implementation start dates (range, June 2010 through June 2017). We identified 23,021 positive C. difficile test results (HO-Lab ID: 5,014) with 16,213 HO-CDI and 24,083,252 BDOC from the 129 hospitals during the study period. There were declining baseline trends nationwide (mean, −0.6% per month) for HO-CDI. The use of UV-C had no statistically significant association with incidence rates of HO-CDI (incidence rate ratio [IRR], 1.032; 95% CI, 0.963–1.106; P = .65) or incidence rates of healthcare-associated positive C. difficile test results (HO-Lab). Conclusions: In this large quasi-experimental analysis within the VHA System, the enhanced terminal room cleaning with UVC disinfection was not associated with the change in incidence rates of clinically confirmed hospital-onset CDI or positive test results with recent healthcare exposure. Further research is needed to understand reasons for lack of effectiveness, such as understanding barriers to utilization.Funding: NoneDisclosures: None

scholarly journals Ultraviolet-C as a Viable Reprocessing Method for Disposable Masks and Filtering Facepiece Respirators

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 801
Author(s):  
Talita Nicolau ◽  
Núbio Gomes Filho ◽  
Andrea Zille
Keyword(s):  
Literature Review ◽  
Systematic Literature Review ◽  
Personal Protective Equipment ◽  
State Of The Art ◽  
Protective Equipment ◽  
Multiple Methods ◽  
Ultraviolet C ◽  
Single Use ◽  
Uv C ◽  
New Strategies

In normal conditions, discarding single-use personal protective equipment after use is the rule for its users due to the possibility of being infected, particularly for masks and filtering facepiece respirators. When the demand for these protective tools is not satisfied by the companies supplying them, a scenario of shortages occurs, and new strategies must arise. One possible approach regards the disinfection of these pieces of equipment, but there are multiple methods. Analyzing these methods, Ultraviolet-C (UV-C) becomes an exciting option, given its germicidal capability. This paper aims to describe the state-of-the-art for UV-C sterilization in masks and filtering facepiece respirators. To achieve this goal, we adopted a systematic literature review in multiple databases added to a snowball method to make our sample as robust as possible and encompass a more significant number of studies. We found that UV-C’s germicidal capability is just as good as other sterilization methods. Combining this characteristic with other advantages makes UV-C sterilization desirable compared to other methods, despite its possible disadvantages.

Sign in / Sign up

Export Citation Format

Share Document